Determination of 3-Methoxytyramine and Adrenaline in Dairy Products by Isotopic Dilution-Assisted Liquid Chromatography-Tandem Mass Spectrometry

BAI Yanmei; ZHANG Lijia; WANG Yang; LIU Lijun; XIE Ruilong; FAN Min; LI Cuizhi

doi:10.13386/j.issn1002-0306.2022110234

Volume 44 Issue 19

Oct. 2023

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Science and Technology of Food Industry > 2023 > 44(19): 305-312. > DOI: 10.13386/j.issn1002-0306.2022110234

BAI Yanmei, ZHANG Lijia, WANG Yang, et al. Determination of 3-Methoxytyramine and Adrenaline in Dairy Products by Isotopic Dilution-Assisted Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2023, 44(19): 305−312. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022110234.

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Determination of 3-Methoxytyramine and Adrenaline in Dairy Products by Isotopic Dilution-Assisted Liquid Chromatography-Tandem Mass Spectrometry

Inner Mongolia Yili Industrial Group Co., Ltd., Hohhot 010110, China

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Received Date: November 23, 2022
Available Online: July 26, 2023

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Abstract

Abstract

A method was established for the simultaneous determination of 3-methoxytyramine and adrenaline in dairy products by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) compled with isotope dilution. Sample was extracted by perchloric acid solution, cleaned by weak cationic solid phase extraction column. The analyte was separated by ACQUITY UPLC BEH C₁₈ (100 mm×2.1 mm, 1.7 μm) column and agradient elution of mobile phase with 0.1% formic acid aqueous solution-methanol. The detection was achieved by HPLC-MS/MS with electrospray ion source and positive ion scanning mode as well as multiple reaction monitoring (MRM) mode. The quantification was accomplished by the internal standard method. It was demonstrated that the linear relationships of 3-methoxytyramine and adrenaline were good in the range of 0.5~12.0 ng/mL and 50~1200 ng/mL (r>0.99), respectively. The detection limits and the limit of quantification of 3-methoxytyramine were 0.2 and 0.5 μg/kg, respectively. At the concentrations of 0.5, 1.0 and 3.0 μg/kg, the recoveries of the 3-methoxytyramine ranged from 76.97% to 119.20%, with the corresponding precision (n=6) of 1.53%~9.61%. While the recoveries of adrenaline at 50, 100 and 300 μg/kg were 90.65%~108.55% and the relative standard deviations were in the range of 1.29%~3.95%. The method would be accurate in results and good in repeatability, and it can be an important measure to monitor 3-methoxytyramine and adrenaline in dairy products.
- liquid chromatography-tandem mass spectrometry,
- dairy product,
- 3-methoxytyramine,
- adrenaline,
- isotope dilution

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