Screening of <i>Fusarium oxysporum</i> for Ethanol Production by Simultaneous Saccharification and Fermentation of Bagasse and Its Ethanol Production Performance

ZHAN Jiajia; ZHOU Quan; XIONG Xingyao; ZHANG Zhixu; HU Yaping; LI Bi; CHEN Zongrui; GONG Yihang; HE Runan; QIN Dan; ZENG Lu

doi:10.13386/j.issn1002-0306.2022110054

Volume 44 Issue 19

Oct. 2023

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Science and Technology of Food Industry > 2023 > 44(19): 108-116. > DOI: 10.13386/j.issn1002-0306.2022110054

ZHAN Jiajia, ZHOU Quan, XIONG Xingyao, et al. Screening of Fusarium oxysporum for Ethanol Production by Simultaneous Saccharification and Fermentation of Bagasse and Its Ethanol Production Performance[J]. Science and Technology of Food Industry, 2023, 44(19): 108−116. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022110054.

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Screening of Fusarium oxysporum for Ethanol Production by Simultaneous Saccharification and Fermentation of Bagasse and Its Ethanol Production Performance

ZHAN Jiajia^{1, 2,},
ZHOU Quan^{1, +,},
XIONG Xingyao³,
ZHANG Zhixu^{4, 5},
HU Yaping¹,
LI Bi¹,
CHEN Zongrui¹,
GONG Yihang¹,
HE Runan¹,
QIN Dan^1, ,,
ZENG Lu^1, ,

1.
College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
2.
Medical & Technology School of Zunyi Medical University, Zunyi 563000, China
3.
Agricultural Genome Research Institute, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
4.
College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China
5.
Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding, Ministry of Education, Hunan Agricultural University, Changsha 410128, China

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Received Date: November 06, 2022
Available Online: August 04, 2023

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Abstract

Abstract

In order to obtain an excellent Fusarium oxysporum strain for simultaneous saccharification and fermentation (SSF), the cellulase activity of the 14 strains of Fusarium oxysporum was determined, and Congo red plate staining and paper disintegrating tests were carried out to screen the strains, the ethanol production experiment of SSF was carried out to investigate its cellulose degradation and sugar conversion performance. The results showed that strain mh2 had strong cellulose degradation ability, its cellulase activity was 14.28 U/mL, the diameter of transparent circle was 4.5 cm, which could completely degrade the filter paper into paste. The yield of ethanol could reach 98 g/kg and 41.7 g/kg, respectively, and the conversion of ethanol could reach up to 33.36%. In addition, the activities of filter paper enzyme, endo-glucanase, β-glucanase and exo-glucanase in mh2 cellulase system were 25.63, 15.69, 19.61 and 22.44 U/mL, respectively, the degradation rate of bagasse cellulose was 25.6%, and the ethanol conversion rate of glucose and xylose was 0.443 and 0.213 g/g, respectively, the biodegradability of mh2 was 1.58, 1.17 and 3.8 times higher than that of Cs20, respectively, indicating that mh2 had good biodegradability and sugar conversion during SSF. This study can provide data for the study of SSF to enrich the resources of SSF strains and single strain to produce ethanol from lignocellulose.
- bagasse,
- Fusarium oxysporum,
- simultaneous saccharification and fermentation,
- cellulose ethanol,
- xylose

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