Research on the Suitability of Teas Made from New Tea Line of Kucha No.6 with High Theacrine

QIN Dandan; CHEN Junli; LI Bo; PAN Chendong; WANG Qiushuang; FANG Kaixing; LI Hongjian; NI Erdong; JIANG Xiaohui; WANG Qing; WU Hualing

doi:10.13386/j.issn1002-0306.2022110038

Volume 44 Issue 17

Aug. 2023

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Science and Technology of Food Industry > 2023 > 44(17): 316-323. > DOI: 10.13386/j.issn1002-0306.2022110038

QIN Dandan, CHEN Junli, LI Bo, et al. Research on the Suitability of Teas Made from New Tea Line of Kucha No.6 with High Theacrine[J]. Science and Technology of Food Industry, 2023, 44(17): 316−323. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022110038.

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Research on the Suitability of Teas Made from New Tea Line of Kucha No.6 with High Theacrine

Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation and Utilization, Guangzhou 510640, China

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Received Date: November 06, 2022
Available Online: July 05, 2023

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Abstract

Abstract

The fresh leaves of Kucha No.6 with high theacrine as raw materials in different seasons were processed into green tea, white tea and black tea. The tea suitability of Kucha No.6 was comprehensively analyzed by sensory evaluation and main quality components. The sensory evaluation results showed that three types of Kucha No.6 tea all had different degrees of bitterness (green tea>white tea>black tea), and the bitterness of spring samples was greater than that of other two seasons. Among of them, green tea had a floral and bean fragrance, a mellow and bitter taste. White tea had a medical and floral fragrance, a mellow taste, and black tea had sweet and rich fragrance, mellow and refresh taste. Additionally, the results of main quality components analysis showed that the contents of some components related to bitterness, such as tea polyphenols, total catechins, EGCG, and EGC, were significantly higher in green and white tea than those in black tea. Furthermore, the contents of theacrine in three types of Kucha No.6 tea in spring were significantly higher than those in other two seasons. According to this study, Kucha No.6 containing high theacrine was more suitable for making black tea, not only because the high contents of theacrine, but also of the characteristics of sweet and rich fragrance, mellow and refresh taste. Although green tea and white tea made by Kucha No.6 had strong bitter taste, they were more suitable for promoting as further processed products because of their high contents of healthcare functional ingredients such as theacrine, EGCG, etc. This study provides more theoretical and practical basis for the further development and utilization of Kucha resources.
- Kucha,
- theacrine,
- processing suitability,
- sensory evaluation,
- quality components

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[1]	秦丹丹, 王秋霜, 李红建, 等. 苦茶及其特异性成分苦茶碱研究进展[J]. 食品科学,2021,42(13):353−359. [QIN D D, WANG Q S, LI H J, et al. Progress in research on Camellia kucha (Chang et Wang) Chang and its special constituent theacrine[J]. Food Science,2021,42(13):353−359. doi: 10.7506/spkx1002-6630-20200617-231 QIN D D, WANG Q S, LI H J, et al. Progress in research on Camellia kucha (Chang et Wang) Chang and its special constituent theacrine[J]. Food Science, 2021, 42(13): 353-359. doi: 10.7506/spkx1002-6630-20200617-231
[2]	王新超, 姚明哲, 马春雷, 等. 我国苦茶资源主要生化成分的鉴定评价[J]. 中国农学通报,2008(6):65−69. [WANG X C, YAO M Z, MA C L, et al. Analysis and evalation of biochemical compenents in bitter tea plant germplasms[J]. Agricultural Science & Technology,2008(6):65−69. WANG X C, YAO M Z, MA C L, et al. Analysis and evalation of biochemical compenents in bitter tea plant germplasms[J]. Agricultural Science & Technology, 2008 (6): 65-69.
[3]	杨超, 马存强, 周斌星, 等. 苦茶特征性成分研究[J]. 昆明学院学报,2014,36(3):75−78. [YANG C, MA C C, ZHOU B X, et al. Characteristic components research in bitter tea[J]. Journal of Kunming University,2014,36(3):75−78. YANG C, MA C C, ZHOU B X, et al. Characteristic components research in bitter tea [J]. Journal of Kunming University, 2014, 36(3): 75-78.
[4]	唐琴, 孙威江, 陈志丹, 等. 尤溪苦茶资源苦涩味物质测定与分析[J]. 食品科学,2019,40(18):242−247. [TANG Q, SUN W J, CHEN Z D, et al. Determination and analysis of bitter and astringent substances in Youxi bitter tea resources[J]. Food Science,2019,40(18):242−247. TANG Q, SUN W J, CHEN Z D, et al. Determination and analysis of bitter and astringent substances in Youxi bitter tea resources[J]. Food Science, 2019, 40(18): 242-247.
[5]	SHENG Y Y, XIANG J, WANG Z S, et al. Theacrine from Camellia kucha and its health beneficial effects[J]. Frontiers in Nutrition,2020,7:596823. doi: 10.3389/fnut.2020.596823
[6]	尚卫琼, 李友勇, 刘悦, 等. 基于EST-SSR标记的西双版纳苦茶资源遗传多样性分析[J]. 山西农业科学,2020,48(2):167−171. [SHANG W Q, LI Y Y, LIU Y, et al. Genetic diversity analysis of bitter tea germplasm resource in Xishuangbanna based on EST-SSR markers[J]. Journal of Shanxi Agricultural Sciences,2020,48(2):167−171. doi: 10.3969/j.issn.1002-2481.2020.02.09 SHANG W Q, LI Y Y, LIU Y, et al. Genetic diversity analysis of bitter tea germplasm resource in Xishuangbanna based on EST-SSR markers[J]. Journal of Shanxi Agricultural Sciences, 2020, 48(2): 167-171. doi: 10.3969/j.issn.1002-2481.2020.02.09
[7]	成杨, 刘振, 赵洋, 等. 江华苦茶的亲缘关系与遗传多样性研究[J]. 茶叶通讯,2019,46(2):141−148. [HENG Y, LIU Z, ZHAO Y, et al. Study on the genetic relationship and genetic diversity of Jianghua bitter tea[J]. Journal of Tea Communication,2019,46(2):141−148. HENG Y, LIU Z, ZHAO Y, et al. Study on the genetic relationship and genetic diversity of Jianghua bitter tea[J]. Journal of Tea Communication, 2019, 46(2): 141-148.
[8]	杨培迪, 刘振, 赵洋, 等. 基于农艺性状和SSR标记亲缘关系分析的江华苦茶品系评价[J]. 分子植物育种,2021,19(7):2402−2409. [YANG P D, LIU Z, ZHAO Y, et al. Evaluation of Jianghua kucha tea strains based on agronomic and SSR molecular marker relationship analysis[J]. Molecular Plant Breeding,2021,19(7):2402−2409. YANG P D, LIU Z, ZHAO Y, et al. Evaluation of Jianghua kucha tea strains based on agronomic and SSR molecular marker relationship analysis[J]. Molecular Plant Breeding, 2021, 19(7): 2402-2409.
[9]	刘振, 杨培迪, 成杨, 等. 江华苦茶新品系红茶发酵性研究[J]. 茶叶通讯,2019,46(4):419−423, 494. [LIU Z, YANG P D, CHENG Y, et al. Study on the fermentability of black tea produced from new strains of Jianghua Kucha[J]. Journal of Tea Communication,2019,46(4):419−423, 494. LIU Z, YANG P D, CHENG Y, et al. Study on the fermentability of black tea produced from new strains of Jianghua kucha[J]. Journal of Tea Communication, 2019, 46(4): 419-423, 494.
[10]	刘玉飞, 庞丹丹, 李友勇, 等. 苦茶碱代谢关键转录因子基因的筛选鉴定[J]. 茶叶科学,2022,42(1):41−50. [LIU Y F, PANG D D, LI Y Y, et al. The screening and identification of key transcription factor genes for theacrine metabolism[J]. Journal of Tea Science,2022,42(1):41−50. LIU Y F, PANG D D, LI Y Y, et al. The screening and identification of key transcription factor genes for theacrine metabolism[J]. Journal of Tea Science, 2022, 42(1): 41-50.
[11]	陈潇敏, 王鹏杰, 王淑燕, 等. 基于转录组挖掘蕉城苦茶苦茶碱合成相关基因[J]. 应用与环境生物学报,2021,27(5):1382−1389. [CHEN X M, WANG P J, WANG S Y, et al. Excavation of genes involved in theacrine biosynthesis of Jiaocheng Kucha based on transcriptome[J]. Chinese Journal of Applied and Environmental Biology,2021,27(5):1382−1389. CHEN X M, WANG P J, WANG S Y, et al. Excavation of genes involved in theacrine biosynthesis of Jiaochengkucha based on transcriptome[J]. Chinese Journal of Applied and Environmental Biology, 2021, 27(5): 1382-1389.
[12]	李红建, 秦丹丹, 姜晓辉, 等. 广东苦茶资源嘌呤生物碱含量分析与评价[J]. 茶叶科学,2021,41(1):71−79. [LI H J, QIN D D, JIANG X H, et al. Analysis and evaluation of purine alkaloid contents in bitter tea germplasm resources from Guangdong[J]. Journal of Tea Science,2021,41(1):71−79. LI H J, QIN D D, JIANG X H, et al. Analysis and evaluation of purine alkaloid contents in bitter tea germplasm resources from Guangdong [J]. Journal of Tea Science, 2021, 41(1): 71-79.
[13]	QIN D, WANG Q, LI H, et al. Identification of key metabolites based on non-targeted metabolomics and chemometrics analyses provides insights into bitterness in Kucha [Camellia kucha (Chang et Wang) Chang][J]. Food Research International,2020,138(Pt B):109789.
[14]	WU W, LU M, PENG J, et al. Nontargeted and targeted metabolomics analysis provides novel insight into nonvolatile metabolites in Jianghua Kucha tea germplasm (Camellia sinensis var. Assamica cv. Jianghua)[J]. Food Chemistry:X,2022,13:100270. doi: 10.1016/j.fochx.2022.100270
[15]	WANG S, CHEN J, MA J, et al. Novel insight into theacrine metabolism revealed by transcriptome analysis in bitter tea (Kucha, Camellia sinensis)[J]. Science Reports,2020,10(1):6286. doi: 10.1038/s41598-020-62859-2
[16]	LI H, FANG K, QIN D, et al. Comparative transcriptome analysis reveals putative genes responsible for high theacrine content in kucha (Camellia kucha (Chang et Wang) Chang)[J]. Tropical Plant Biology,2021,14(1):82−92. doi: 10.1007/s12042-020-09280-1
[17]	ZHANG Y H, LI Y F, WANG Y, et al. Identification and characterization of N9-methyltransferase involved in converting caffeine into non-stimulatory theacrine in tea[J]. Nature Communication,2020,11(1):1473. doi: 10.1038/s41467-020-15324-7
[18]	ZHENG X Q, YE C X, KATO M, et al. Theacrine (1,3,7,9-tetramethyluric acid) synthesis in leaves of a Chinese tea, Kucha (Camellia assamica var. kucha)[J]. Phytochemistry,2002,60(2):129−134. doi: 10.1016/S0031-9422(02)00086-9
[19]	GB/T 23776-2018, 茶叶感官审评方法[S]. 北京: 中国标准出版社, 2018. GB/T 23776-2018, Methodology for sensory evaluation of tea[S]. Beijing: China Quality and Standards Publishing & Media Co., Ltd., 2018.
[20]	王秋霜, 曹冰冰, 王青, 等红紫芽品种的茶类适制性研究[J]. 食品工业科技, 2022, 43(15): 279-288. WANG Q S, CAO B B, WAGN Q, et al. Research on the suitability of teas made from fresh tea leaves of purple tea[J]. Science and Technology of Food Industry, 2022, 43(15): 279-288.
[21]	方开星, 姜晓辉, 秦丹丹, 等. 高氨基酸和高茶氨酸茶树资源筛选[J]. 核农学报,2019,33(9):1724−1733. [FANG K X, JIANG X H, QIN D D, et al. Selection of tea germplasm with high contents of amino acid and theanine[J]. Journal of Nuclear Agricultural Sciences,2019,33(9):1724−1733. FANG K X, JIANG X H, QIN D D, et al. Selection of tea germplasm with high contents of amino acid and theanine [J]. Journal of Nuclear Agricultural Sciences, 2019, 33(9): 1724-1733.
[22]	时鸿迪, 王邦政, 李乾, 等. 不同加工工艺下‘紫娟’茶品质的比较[J]. 中国农学通报,2020,36(34):142−147. [SHI H D, WANG B Z, LI Q, et al. The quality of ‘Zijuan’ tea under different processing techniques: A comparative analysis[J]. Chinese Agricultural Science Bulletin,2020,36(34):142−147. SHI H D, WANG B Z, LI Q, et al. The quality of ‘Zijuan’ tea under different processing techniques: A comparative analysis[J]. Chinese Agricultural Science Bulletin, 2020, 36(34): 142-147.
[23]	杨双旭, 周炎花, 朱元培, 等. 漳州野生茶不同茶类加工工艺及品质分析[J]. 热带农业科学,2020,40(5):83−87. [YANG S X, ZHOU Y H, ZHU Y P, et al. Analysis of processing technology and quality of wild tea in Zhangzhou[J]. Chinese Journal of Tropical Agriculture,2020,40(5):83−87. YANG S X, ZHOU Y H, ZHU Y P, et al. Analysis of processing technology and quality of wild tea in Zhangzhou[J]. Chinese Journal of Tropical Agriculture, 2020, 40(5): 83-87.
[24]	ROBICHAUD J, NOBLE A. Astringency and bitterness of selected phenolics in wine[J]. Journal of the Science of Food and Agriculture,1990,53:343−353. doi: 10.1002/jsfa.2740530307
[25]	吴小清. 茶树品种‘栗峰’的生化成分和加工品质特性研究[D]. 杭州: 浙江大学, 2020. WU X B. Biochemical and quality characters of ‘Lifeng’ cultivar using different tea processing methods[D]. Hangzhou: Zhejiang University, 2020.
[26]	张燕红. 典型酚酸类化合物的呈味特性及对绿茶茶汤苦涩味的影响[D]. 合肥: 安徽农业大学, 2019. ZHANG Y H. The taste characteristics of typical phenolic acids and their effects on the bitterness and astringency of green tea infusion[D]. Hefei: Anhui Agricultural University, 2019.
[27]	叶创兴, 林永成, 苏建业, 等. 苦茶Camellia assamica var kucha Chang et Wang的嘌呤生物碱[J]. 中山大学学报(自然科学版),1999(5):82−86. [YE C X, LIN Y C, SU J Y, et al. Purine alkaloids in Camellia assamica var. kucha Chang et Wang[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni,1999(5):82−86. YE C X, LIN Y C, SU J Y, et al. Purine alkaloids in Camellia assamica var. kucha Chang et Wang [J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 1999(5): 82-86.
[28]	ZHANG Y N, YIN J F, CHEN J X, et al. Improving the sweet aftertaste of green tea infusion with tannase[J]. Food Chemistry,2016,192:470−476. doi: 10.1016/j.foodchem.2015.07.046
[29]	李鑫磊, 俞晓敏, 龚智宏, 等. 绿茶、红茶、乌龙茶和白茶中主要代谢产物的差异[J]. 福建农林大学学报(自然科学版),2019,48(5):559−566. [LI X L, YU X M, GONG Z H, et al. Difference in main metabolites contents in green tea, black tea, oolong tea and white tea[J]. Journal of Fujian Agriculture and Forestry University (Natural Science Edition),2019,48(5):559−566. LI X L, YU X M, GONG Z H, et al. Difference in main metabolites contents in green tea, black tea, oolong tea and white tea[J]. Journal of Fujian Agriculture and Forestry University(Natural Science Edition) , 2019, 48(5): 559-566.
[30]	卢莉, 程曦, 叶国盛, 等. 4种乌龙茶树鲜叶适制绿茶、黄茶、白茶、红茶可行性研究[J]. 食品工业科技,2020,41(2):33−38. [LU L, CHENG X, YE G, et al. Feasibility of green tea, yellow tea, white tea and black tea made from the fresh leaves of four oolong tea varieties[J]. Science and Technology of Food Industry,2020,41(2):33−38. LU L, CHENG X, YE G, et al. Feasibility of green tea, yellow tea, white tea and black tea made from the fresh leaves of four oolong tea varieties[J]. Science and Technology of Food Industry, 2020, 41(2): 33-38.
[31]	魏沙沙, 彭静, 陈志丹, 等. 尤溪苦茶苦味相关物质检测及与苦味的关联分析[J]. 茶叶科学,2021,41(3):337−349. [WEI S S, PENG J, CHEN Z D, et al. Detection of bitterness-related substances in Youxi bitter tea and correlation analysis with bitterness[J]. Journal of Tea Science,2021,41(3):337−349. doi: 10.3969/j.issn.1000-369X.2021.03.005 WEI S S, PENG J, CHEN Z D, et al. Detection of bitterness-related substances in Youxi bitter tea and correlation analysis with bitterness[J]. Journal of Tea Science, 2021, 41(3): 337-349. doi: 10.3969/j.issn.1000-369X.2021.03.005
[32]	GUO X, SONG C, HO C T, et al. Contribution of l-theanine to the formation of 2, 5-dimethylpyrazine, a key roasted peanutty flavor in Oolong tea during manufacturing processes[J]. Food Chemistry,2018,263:18−28. doi: 10.1016/j.foodchem.2018.04.117
[33]	谢关华. 不同加工方式对茶叶风味物质和体外生物活性的影响研究[D]. 重庆: 西南大学, 2021. XIE G H. Study on the effects of different processing methods on tea flavor compounds and bioactivity in vitro[D]. Chongqing: Southwest University, 2021.
[34]	乔小燕, 吴华玲, 韩雪文, 等. 仁化白毛茶生化成分与成品白茶品质的相关性研究[J]. 核农学报,2015,29(12):2327−2333. [QIAO X Y, WU H L, HAN X W, et al. Correlation on biochemical components and made-tea quality of Renhua Baimaocha[J]. Journal of Nuclear Agricultural Sciences,2015,29(12):2327−2333. doi: 10.11869/j.issn.100-8551.2015.12.2327 QIAO X Y, WU H L, HAN X W, et al. Correlation on biochemical components and made-tea quality of Renhua baimaocha[J]. Journal of Nuclear Agricultural Sciences, 2015, 29(12): 2327-2333. doi: 10.11869/j.issn.100-8551.2015.12.2327
[35]	方奇挺, 金晶, 朱雨梦, 等. 覆盖遮荫及生产季节对蒸青绿茶滋味和香气物质的影响[J]. 茶叶,2021,47(3):131−137. [FNAG Q T, JIN J, ZHU Y M, et al. Effect of shade treatment and harvest season on the taste and aroma-related components in steamed green tea[J]. Journal of Tea,2021,47(3):131−137. doi: 10.3969/j.issn.0577-8921.2021.03.001 FNAG Q T, JIN J, ZHU Y M, et al. Effect of shade treatment and harvest season on the taste and aroma-related components in steamed green tea [J]. Journal of Tea, 2021, 47(3): 131-137. doi: 10.3969/j.issn.0577-8921.2021.03.001

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