WANG Yuhui, ZHANG Jinfeng, LI Haokun, et al. Effects of pH on the Physicochemical Properties of Common Vetch (Vicia sativa L) Albumin Protein and Globulin Protein[J]. Science and Technology of Food Industry, 2023, 44(17): 76−83. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022110019.
Citation: WANG Yuhui, ZHANG Jinfeng, LI Haokun, et al. Effects of pH on the Physicochemical Properties of Common Vetch (Vicia sativa L) Albumin Protein and Globulin Protein[J]. Science and Technology of Food Industry, 2023, 44(17): 76−83. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022110019.

Effects of pH on the Physicochemical Properties of Common Vetch (Vicia sativa L) Albumin Protein and Globulin Protein

More Information
  • Received Date: November 01, 2022
  • Available Online: July 03, 2023
  • This study focused on the physicochemical properties of the common vetch albumin protein (CVAP) and globulin protein (CVGP) from CV seeds under different pH (2.0~13.0) conditions. The molecular weight composition, surface hydrophobicity, Zeta-potential, solubility, emulsifying and foaming properties were studied. Results revealed that the secondary structure, the surface hydrophobicity and Zeta-potential of proteins experienced dynamic changes. These changes were possibly related to the different compositions of proteins as shown by the SDS-PAGE profiles. Meanwhile, the solubility, emulsifying and foaming properties of CVAP and CVGP were significantly changed under different pH treatments, respectively. A lower solubility in CVAP under pH<5.0 and a higher solubility in CVAP under pH>7.0 than that in CVGP were observed, respectively. The emulsifying and foaming properties of CVAP and CVGP under pH11.0~12.0 treatments were superior to those under other pH. In conclusion, the physicochemical properties of common vetch protein can be changed by different pH treatments. Moreover, a better emulsifying and foaming properties of CVAP and CVGP can be obtained under pH11.0~12.0 treatments.
  • [1]
    付丽平. 箭筈豌豆淀粉和蛋白质性质的研究[D]. 青岛: 青岛科技大学, 2020

    FU Liping. Studies on the properties of starches and proteins from common vetches[D]. Qingdao: Qingdao University of Science and Technology, 2020.
    [2]
    RIBEIRO A C, TEIXEIRA A A R, FERREIRA R B. Characterization of globulins from common vetch (Vicia sativa L.)[J]. Journal of Agricultural and Food Chemistry,2004,52(15):4913−4920. doi: 10.1021/jf049833p
    [3]
    CHEN W, WANG Y, LÜ X, et al. Physicochemical, structural and functional properties of protein isolates and major protein fractions from common vetch (Vicia sativa L.)[J]. International Journal of Biological Macromolecules,2022,216(9):487−497.
    [4]
    陈旺. 箭筈豌豆蛋白理化功能特性及其水解肽螯合钙机制的研究[D]. 青岛: 青岛科技大学, 2022

    CHEN Wang. Studies on the physicochemical and functional properties of common vetch protein and the mechanism of its calcium-binding peptide of combination[D]. Qingdao: Qingdao University of Science and Technology, 2022.
    [5]
    AMAGLIANI L O, REGAN J, KELLY A L, et al. The composition, extraction, functionality and applications of rice proteins: A review[J]. Trends in Food Science and Technology,2017,64(6):1−12.
    [6]
    吴晓娟, 王晓婵, 张佳妮, 等. pH值碱性偏移结合热处理对米糠蛋白结构和功能性质的影响[J]. 食品科学,2021,42(4):23−30. [WU Xiaojuan, WANG Xiaochan, ZHANG Jiani, et al. Effect of alkaline pH-shifting combined with heat treatment on the structural and functional properties of rice bran protein[J]. Food Science,2021,42(4):23−30.

    WU Xiaojuan, WANG Xiaochan, ZHANG Jiani, et al. Effect of alkaline pH-shifting combined with heat treatment on the structural and functional properties of rice bran protein[J]. Food Science, 2021, 42(4): 23-30.
    [7]
    魏冬旭, 江连洲, 王辰, 等. pH值对大豆11S球蛋白结构和表面疏水性的影响[J]. 食品科学,2015,36(11):1−5. [WEI Dongxu, JIANG Lianzhou, WANG Chen, et al. Influence of pH on structure and surface hydrophobicity of glycinin[J]. Food Science,2015,36(11):1−5. doi: 10.7506/spkx1002-6630-201511001

    WEI Dongxu, JIANG Lianzhou WANG Chen, et al. Influence of pH on structure and surface hydrophobicity of glycinin[J]. Food Science, 2015, 36(11): 1-5. doi: 10.7506/spkx1002-6630-201511001
    [8]
    曾琪, 胡淼, 王欢, 等. pH值处理对黑豆分离蛋白结构, 流变特性及乳化性能的影响[J]. 食品科学,2020,41(22):15−21. [ZENG Qi, HU Miao, WANG Huan, et al. Effect of pH treatment on structure, rheological properties and emulsifying properties of black bean protein isolate[J]. Food Science,2020,41(22):15−21. doi: 10.7506/spkx1002-6630-20190906-080

    ZENG Qi, HU Miao, WANG Huan, et al. Effect of pH treatment on structure, rheological properties and emulsifying properties of black bean protein isolate [J]. Food Science, 2020, 41(22): 15-21. doi: 10.7506/spkx1002-6630-20190906-080
    [9]
    孙媛. 改良Osborne法分级分离四种小麦蛋白的研究[D]. 广州: 华南理工大学, 2015

    SUN Yuan. Modification of Osborne method for fractionation of four wheat bran proteins[D]. Guangzhou: South China University of Technology, 2015.
    [10]
    杨珊, 蔡秀琴, 刘雨晗, 等. 液体物质红外光谱的简便测试方法研究[J]. 光谱学与光谱分析,2022,42(7):2143−2147. [YANG Shan, CAI Xiuqin, LIU Yuhan, et al. A simple method for measuring infrared spectra of liquid substances[J]. Spectroscopy and Spectral Analysis,2022,42(7):2143−2147.

    YANG Shan, CAI Xiuqin, LIU Yuhan, et al. A simple method for measuring infrared spectra of liquid substances[J]. Spectroscopy and Spectral Analysis, 2022, 42(7): 2143-2147.
    [11]
    刘爱成, 李墨翰, 张正翰, 等. 牛乳, 驴乳乳清蛋白二级结构及其功能对比研究[J]. 乳业科学与技术,2021,44(3):6−11. [LIU Aicheng, LI Mohan, ZHANG Zhenghan, et al. Comparative study on secondary structure and function of whey protein in bovine milk and donkey milk[J]. Journal of Dairy Science and Technology,2021,44(3):6−11.

    LIU Aicheng, LI Mohan, ZHANG Zhenghan, et al. Comparative study on secondary structure and function of whey protein in bovine milk and donkey milk[J]. Journal of Dairy Science and Technology, 2021, 44(3): 6-11.
    [12]
    LAEMMLI U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J]. Nature,1970,227(5259):680−685. doi: 10.1038/227680a0
    [13]
    KATO A, NAKAI S. Hydrophobicity determination by a fluorescence probe method and its correlation with surface properties of proteins[J]. Biochimica et Biophysica Acta,1980,624(1):13−20. doi: 10.1016/0005-2795(80)90220-2
    [14]
    DENG Y J, HUANG L X, ZHANG H C, et al. Physicochemical and functional properties of Chinese quince seed protein isolate[J]. Food Chemistry,2019,283(6):539−548.
    [15]
    DMITRII U, VERA S, ANDREY K, et al. FTIR spectroscopy study of the secondary structure changes in human serum albumin and trypsin under neutral salts[J]. Biomolecules,2020,10(4):606−606. doi: 10.3390/biom10040606
    [16]
    FEN N F, SINGH B R, DEOLIVEIRA D B, et al. Secondary structure estimation of proteins using the amide III region of fourier transform infrared spectroscopy: Application to analyze calcium-binding-induced structural changes in calsequestrin[J]. Applied Spectroscopy,1994,48(11):1432−1441. doi: 10.1366/0003702944028065
    [17]
    刘锞琳, 何悦珊, 王钊, 等. 傅里叶红外与拉曼光谱法测定蛋白质二级结构研究进展[J/OL]. 食品与发酵工业. 2022: 1−8. https://doi.org/10.13995/j.cnki.11-1802/ts.031883.

    LIU Kelin, HE Yueshan, WANG Zhao, et al. Progress in determination of protein secondary structure by Fourier infrared spectroscopy and Raman spectroscopy[J/OL]. Food and Fermentation Industries. 2022: 1−8. https://doi.org/10.13995/j.cnki.11-1802/ts.031883.
    [18]
    张唯唯, 何振东, 马天怡, 等. 极端酸碱pH偏移改善白果蛋白溶解性和乳化性[J]. 精细化工,2021,38(6):1204−1211. [ZHANG Weiwei, HE Zhengdong, MA Tianyi, et al. Extreme acid and alkaline pH-shifting processes improving the solubility and emulsifying properties of Ginkgo seed protein isolate[J]. Fine Chemicals,2021,38(6):1204−1211. doi: 10.13550/j.jxhg.20201155

    ZHANG Weiwei, HE Zhengdong, MA Tianyi, et al. Extreme acid and alkaline pH-shifting processes improving the solubility and emulsifying properties of Ginkgo seed protein isolate [J]. Fine Chemicals, 2021, 38(6): 1204-1211. doi: 10.13550/j.jxhg.20201155
    [19]
    GODBEY W T. An introduction to biotechnology applications: The science, technology and medical applications[M]. London: Academic Press, 2015: 9−33.
    [20]
    ZHANG A Q, CHEN S, WANG Y Y, et al. Effect of different homogenization pressure on soy protein isolate-vitamin D3 complex[J]. Process Biochemistry,2019,87(12):145−150.
    [21]
    YAO F, CHEN F, DU Y, et al. Functional and structural properties of soy 11S globulin: Influence of reverse micelle extraction[J]. Journal of Food Science,2021,86(7/9):3403−3412.
    [22]
    巨倩. 不同比例大豆7S, 11S球蛋白水分散体系性质的研究[D]. 咸阳: 西北农林科技大学, 2019

    JU Qian. Study on the properties of water dispersion system of soybean 7S and 11S globulin in different ratios[D]. Xianyang: Northwest Agriculture & Forestry University, 2019.
    [23]
    CHEUNG L, WANASUNDARA J, NICKERSON M T. Effect of pH and NaCl on the emulsifying properties of a napin protein isolate[J]. Food Biophysics,2015,10(1):1−9. doi: 10.1007/s11483-014-9382-z
    [24]
    LIN S, LIN Y, CHEN H. Low molecular weight chitosan prepared with the aid of cellulase, lysozyme and chitinase: Characterisation and antibacterial activity[J]. Food Chemistry,2009,116(1):47−53. doi: 10.1016/j.foodchem.2009.02.002
    [25]
    王琳, 周国卫, 于志超, 等. pH值偏移处理对油莎豆蛋白结构及乳化性质的影响[J]. 食品科学,2020,41(22):34−41. [WANG L, ZHOU G W, YU Z C, et al. Effect of pH-shifting treatment on the structure and emulsifying properties of Cyperus esculentus L. protein[J]. Food Science,2020,41(22):34−41. doi: 10.7506/spkx1002-6630-20191014-113

    WANG L, ZHOU G W, YU Z C, et al. Effect of pH-shifting treatment on the structure and emulsifying properties of Cyperus esculentus L. protein[J]. Food Science, 2020, 41(22): 34-41. doi: 10.7506/spkx1002-6630-20191014-113
    [26]
    TIAN T, TAHA A, ZHANG P, et al. Effects of protein concentration, pH, and NaCl concentration on the physicochemical, interfacial, and emulsifying properties of β-conglycinin[J]. Food Hydrocolloids,2021,118(9):106784.1−106784.8.
    [27]
    李儒仁, 杨鹏, 荣良燕, 等. Chaotropic离子对肌球蛋白乳化特性影响的研究进展[J]. 肉类研究,2018,32(9):47−54. [LI R R, YANG P, RONG L Y, et al. Recent advances in understanding the effect of Chaotropic iron on emulsifying properties of myofibrillar proteins[J]. Meat Research,2018,32(9):47−54.

    [LI R R, YANG P, RONG L Y, et al. Recent advances in understanding the effect of chaotropic iron on emulsifying properties of myofibrillar proteins[J]. Meat Research, 2018, 32(9): 47-54.
    [28]
    DING L, XIA M, ZENG Q, et al. Foaming properties and aggregation mechanism of egg white protein with different physical treatments[J]. LWT-Food Science and Technology,2022,153(2):112505−112515.
    [29]
    LI J, YANG X, SWALLAH M S, et al. Soy protein isolate: An overview on foaming properties and air-liquid interface[J]. International Journal of Food Science and Technology,2022,57(1):188−200. doi: 10.1111/ijfs.15390
    [30]
    DACHMANN E, NOBIS V, KULOZIK U, et al. Surface and foaming properties of potato proteins: Impact of protein concentration, pH value and ionic strength[J]. Food Hydrocolloids,2020,107(10):105981−105993.
  • Cited by

    Periodical cited type(10)

    1. 舒丽枝,时苗苗,张牧焓,卞欢,徐为民,王道营. 卟啉类化合物和游离铁对鸡胸肉肌原纤维蛋白理化特性的影响. 江苏农业学报. 2024(10): 1952-1961 .
    2. 王晓芸,高霞,尤娟,尹涛,刘茹. 超声预处理对鲜湿鱼粉品质的影响及其作用机制. 食品科学. 2024(23): 213-220 .
    3. 韩馨蕊,李颖,刘苗苗,范鑫,冯莉,曹云刚. 安石榴苷与焦磷酸钠对肌原纤维蛋白氧化稳定性及凝胶性能的影响. 食品科学. 2022(08): 15-21 .
    4. 莫玲,香庆文,李晶晶,叶玉萍,赵超超. 孕哺期摄入氧化乳蛋白对子代小鼠机体氧化还原状态的影响. 食品科学技术学报. 2021(03): 122-128 .
    5. 梁恽红,卢涵,张香美. 蛋白二、三级结构对鱼糜凝胶质构和持水力的影响及其测定方法研究进展. 东北农业大学学报. 2021(10): 87-96 .
    6. 谢晨,熊泽语,李慧,金素莱曼,陈百科,包海蓉. 金针菇多糖对三文鱼片冻藏期间品质的影响. 食品与发酵工业. 2021(22): 178-183 .
    7. 刘芳芳,林婉玲,李来好,吴燕燕,杨少玲,黄卉,杨贤庆,林织. 海鲈鱼糜加工及凝胶形成过程中蛋白质的变化机理. 食品科学. 2020(14): 15-22 .
    8. 冯程,Manonose Tariro Upenyu,李志豪,王萍,余雄伟,付琴利,李述刚. 丙烯醛对籽瓜种仁蛋白质结构及凝胶特性影响研究. 食品科技. 2019(09): 66-71 .
    9. 刁小琴,关海宁,李杨,刘丽美. 高压均质对肌原纤维蛋白乳化特性及结构的影响. 食品与发酵工业. 2019(18): 107-112 .
    10. 郭兆斌,马纪兵,张丽,陈骋,陈立业,刘勇,韩玲,余群力. 传统风干牦牛肉加工过程中肌原纤维蛋白氧化对氨基酸的影响. 食品与发酵工业. 2019(22): 202-207+212 .

    Other cited types(4)

Catalog

    Article Metrics

    Article views (143) PDF downloads (28) Cited by(14)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return