Optimization of Ultrasonic-assisted Two-aqueous Extraction of Flavonoids from <i>Rosa roxburghii</i> Tratt and Its Inhibitory Effect on Xanthine Oxidase Activity

ZHUANG Qianfei; LIU Dandan; CHEN Zeyu; SHANG Zufei; LIU Xiaoyan; MA Lizhi

doi:10.13386/j.issn1002-0306.2022100316

Volume 44 Issue 17

Aug. 2023

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Science and Technology of Food Industry > 2023 > 44(17): 222-230. > DOI: 10.13386/j.issn1002-0306.2022100316

ZHUANG Qianfei, LIU Dandan, CHEN Zeyu, et al. Optimization of Ultrasonic-assisted Two-aqueous Extraction of Flavonoids from Rosa roxburghii Tratt and Its Inhibitory Effect on Xanthine Oxidase Activity[J]. Science and Technology of Food Industry, 2023, 44(17): 222−230. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100316.

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Optimization of Ultrasonic-assisted Two-aqueous Extraction of Flavonoids from Rosa roxburghii Tratt and Its Inhibitory Effect on Xanthine Oxidase Activity

ZHUANG Qianfei^1,,
LIU Dandan¹,
CHEN Zeyu¹,
SHANG Zufei¹,
LIU Xiaoyan^{1, 2, 3},
MA Lizhi^{1, 2, 3, ,}

1.
School of Food and Pharmaceutical Engineering, Guiyang University, Guiyang 550005, China
2.
Guizhou Fruit Processing Engineering Technology Research Center, Guiyang 550005, China
3.
Guizhou Collaborative Innovation Center of Fruit Processing, Storage and Safety Control, Guiyang 550005, China

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Received Date: November 01, 2022
Available Online: July 19, 2023

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Abstract

Abstract

In this study, ultrasound-assisted aqueous two-phase extraction (UA-ATPE) was adopted to extract flavonoids from Rosa roxburghii Tratt and investigated its inhibitory effect of this flavonoid on xanthine oxidase activity in vitro. In this experiment, flavonoids yield of Rosa roxburghii Tratt was taken as the index to verify the single factor and response surface optimization of flavonoids extraction process. The optimal extraction process was obtained as follows: Under the condition of 32% C₂H₅OH-20% (NH₄)₂SO₄ two-phase aqueous extraction system, the yield of flavonoids was 140.57±1.78 mg/g when the solid-liquid ratio was 1:59 (g/g), the ultrasonic time was 30 min, the temperature was 50 ℃, and the power was 320 W. Under these conditions, the IC₅₀ value of xanthine oxidase was 12.72 mg/mL in vitro, indicating a good inhibitory activity. The experiment showed that the optimized technology of UA-ATPE extraction of flavonoids from Rosa roxburghii Tratt effectively improved the yield of flavonoids from Rosa roxburghii Tratt. And the flavonoids extracted from Rosa roxburghii Tratt had a good effect of inhibiting the activity of xanthine oxidase and could be used as a natural xanthine oxidase inhibitor.
- Rosa roxburghii flavonoids,
- aqueous two-phase extraction,
- ultrasonic,
- xanthine oxidase

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