Optimization of Extraction Process and Analysis of Monosaccharide Composition of <i>β</i>-1,3-xylan from <i>Caulerpa lentillifera</i>

LIU Qian; JIN Wenhui; JIAO Haotian; XIE Quanling; ZHANG Yiping; HONG Zhuan; ZHAO Yuanhui

doi:10.13386/j.issn1002-0306.2022100086

Volume 44 Issue 16

Aug. 2023

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Science and Technology of Food Industry > 2023 > 44(16): 210-217. > DOI: 10.13386/j.issn1002-0306.2022100086

LIU Qian, JIN Wenhui, JIAO Haotian, et al. Optimization of Extraction Process and Analysis of Monosaccharide Composition of β-1,3-xylan from Caulerpa lentillifera[J]. Science and Technology of Food Industry, 2023, 44(16): 210−217. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100086.

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Optimization of Extraction Process and Analysis of Monosaccharide Composition of β-1,3-xylan from Caulerpa lentillifera

LIU Qian^{1, 2,},
JIN Wenhui^{1, 3, ,},
JIAO Haotian^{1, 2},
XIE Quanling^{1, 3},
ZHANG Yiping^{1, 3},
HONG Zhuan^{1, 3},
ZHAO Yuanhui^2, ,

1.
Technical Innovation Center for Utilization of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China
2.
Sanya Institute of Oceanography, Ocean University of China, Sanya 572024, China
3.
Xiamen Ocean Vocational College, Xiamen 361002, China

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Received Date: October 11, 2022
Available Online: June 22, 2023

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Abstract

Abstract

This study screened several common economic algae to optimize the extraction process of β-1,3-xylan and analyze its monosaccharide composition. The extraction process of crude xylan was optimized by alkaline extraction method, and the monosaccharide composition of crude xylan was identified by a specific enzymatic method using Caulerpa lentillifera as the main raw material. The effect of extraction time, solid-liquid ratio and NaOH concentration on the yield of crude xylan was optimized with a single factor tests followed by three-three level orthogonal experiment to study the optimal extraction conditions of crude xylan. The results showed that the extraction rate of crude xylan was influenced by the following factors: Extraction time>material-to-liquid ratio>NaOH concentration. The optimum extraction process was: Extraction time of 3 h, NaOH concentration of 2.5 mol·L⁻¹, feed-to-liquid ratio of 1:100 g·mL⁻¹, and the yield of crude xylan was 28.1%±0.6%. The enzymatic products were determined by thin-layer chromatography, which showed that the crude xylan extracted from Caulerpa lentillifera was β-1,3-xylan and the monosaccharide fraction was β-1,3-xylose.
- algael,
- Caulerpa lentillifera,
- β-1,3-xylan,
- alkali extraction,
- extraction process,
- β-1,3-xylanase (Xyl3088)

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References

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Science and Technology of Food Industry

Optimization of Extraction Process and Analysis of Monosaccharide Composition of β-1,3-xylan from Caulerpa lentillifera

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