LIU Weiqi, DENG Yuanyuan, WEI Zhencheng, et al. Optimization of Rice Bran Protein Extraction Processing by Response Surface Methodology with Natural Deep Eutectic Solvents[J]. Science and Technology of Food Industry, 2023, 44(17): 194−201. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100049.
Citation: LIU Weiqi, DENG Yuanyuan, WEI Zhencheng, et al. Optimization of Rice Bran Protein Extraction Processing by Response Surface Methodology with Natural Deep Eutectic Solvents[J]. Science and Technology of Food Industry, 2023, 44(17): 194−201. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100049.

Optimization of Rice Bran Protein Extraction Processing by Response Surface Methodology with Natural Deep Eutectic Solvents

More Information
  • Received Date: October 08, 2022
  • Available Online: July 04, 2023
  • In this text, rice bran protein (RBP) was extracted from rice bran using natural deep eutectic solvents (NADES), as well as the extraction processing of parameters were systemically optimized. RBP was characterized by fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Results showed that, the optimization extraction of RBP parameters were carried out as follows: Proline-glycerol (molar ratio 2:5), 4.7% of water in system, solid-liquid ratio of 9:30 g/g for rice bran to NADES, reaction temperature at 65 ℃ for 3.0 h. The maximum extraction efficiency of RBP was 82.69% under the aforementioned conditions. The new chemical compound in the extraction processing of RBP were not able to be observed in terms of FTIR. The micrograph structure of rice bran was effectively damaged as shown in SEM, which might be a reasonable answer for the better extraction efficiency of RBP in NADES. The results in the present work guided for extraction of plant-based proteins with a designed NADES.
  • [1]
    KIM J S, GODBER J S, KING J M, et al. Inhibition of cholesterol autoxidation by the nonsaponifiable fraction in rice bran in an aqueous model system[J]. Journal of the American Oil Chemists' Society,2001,78(7):685−689. doi: 10.1007/s11746-001-0326-8
    [2]
    王永斌. 米糠中功能性成分的研究现状与发展趋势[J]. 中国食物与营养,2006(5):17−20. [WANG Y B. Research status and development trend of functional components in rice bran[J]. Food and Nutrition in China,2006(5):17−20. doi: 10.3969/j.issn.1006-9577.2006.05.005

    WANG Y B. Research status and development trend of functional components in rice bran[J]. Food and Nutrition in China, 2006, (5): 17-20. doi: 10.3969/j.issn.1006-9577.2006.05.005
    [3]
    WU W, LI F, WU X J. Effects of rice bran rancidity on oxidation, structural characteristics and interfacial properties of rice bran globulin[J]. Food Hydrocolloids,2021,110:1−8.
    [4]
    陈正行, 周彤. 米糠: 一种潜在的健康食品优质原料[J]. 粮食与饲料工业,1999(10):40−43. [CHEN Z X, ZHOU T. Rice bran: A potential and healthful foodstuff[J]. Cereal & Feed Industry,1999(10):40−43.

    CHEN Z X, ZHOU T. Rice bran: a potential and healthful foodstuff[J]. Cereal & Feed Industry, 1999, (10): 40-43.
    [5]
    TANG S H, HETTIARACHCHY N S, SHELLHAMMER T H. Protein extraction from heat-stabilized defatted rice bran. 1. Physical processing and enzyme treatments[J]. Journal of Agricultural and Food Chemistry,2002,50(25):7444−7448. doi: 10.1021/jf025771w
    [6]
    SUN L H, LV S W, HE L Y. Comparison of different physical technique-assisted alkali methods for the extraction of rice bran protein and its characterizations[J]. International Journal of Food Engineering,2017,13(10):70−71.
    [7]
    谢宇霞, 吴家乾, 黄玉, 等. 碱法提取米糠中粗蛋白的几个影响因素探讨[J]. 粮食加工,2022,47(3):34−36. [XIE Y X, WU J Q, HUANG Y, et al. Discussion on several influencing factors of alkaline extraction of crud protein from rice bran[J]. Grain Processing,2022,47(3):34−36. doi: 10.3969/j.issn.1007-6395.2022.3.xblykj202203010

    XIE Y X, WU J Q, HUANG Y, et al. Discussion on several influencing factors of alkaline extraction of crud protein from rice bran[J]. Grain Processing, 2022, 47(3): 34-36. doi: 10.3969/j.issn.1007-6395.2022.3.xblykj202203010
    [8]
    RAJCHMAN M, GUTIERREZ M B, CURUTCHET A, et al. Green extraction of rice bran proteins and full revaluation of the remaining by-products[J]. Cereal Chemistry,2021,99(1):184−193.
    [9]
    付珊珊, 周显青, 张玉荣, 等. 超声辅助酶法和碱法对米糠蛋白提取率的影响研究[J]. 食品科技,2021,46(9):149−155. [FU S S, ZHOU X Q, ZHANG Y R, et al. Study on the effect of ultrasound-assisted enzymatic and alkaline methods on the extraction rate of rice bran protein[J]. Food Science and Technology,2021,46(9):149−155. doi: 10.13684/j.cnki.spkj.2021.09.025

    FU S S, ZHOU X Q, ZHANG Y R, et al. Study on the effect of ultrasound-assisted enzymatic and alkaline methods on the extraction rate of rice bran protein[J]. Food Science and Technology, 2021, 46(9): 149-155. doi: 10.13684/j.cnki.spkj.2021.09.025
    [10]
    SUN L H, YU F, WANG Y Y, et al. Effects of ultrasound extraction on the physicochemical and emulsifying properties of rice bran protein[J]. International Journal of Food Engineering,2021,17(5):327−335. doi: 10.1515/ijfe-2019-0115
    [11]
    BEDIN S, ZANELLA K, BRAGAGNOLO N, et al. Implication of microwaves on the extraction process of rice bran protein[J]. Brazilian Journal of Chemical Engineering,2019,36(4):1653−1665. doi: 10.1590/0104-6632.20190364s20180599
    [12]
    易佳, 刘昆仑. 超微联合超声波优化提取米糠蛋白及其对米糠蛋白溶解性的影响[J]. 食品研究与开发,2022,43(19):117−123. [YI J, LIU K L. Optimization of extraction process of rice bran protein by ultrafine grinding combined with ultrasound and effect of extraction on protein solubility[J]. Food Research and Development,2022,43(19):117−123.

    YI J, LIU K L. Optimization of extraction process of rice bran protein by ultrafine grinding combined with ultrasound and effect of extraction on protein solubility[J] Food Research and Development, 2022: 1-11.
    [13]
    曹慧英, 李航, 段庆松, 等. 米糠蛋白提取工艺研究进展[J]. 食品工业,2021,42(7):280−283. [CAO H Y, LI H, DUAN Q S, et al. Research progress on extraction technology of rice bran protein[J]. The Food Industry,2021,42(7):280−283.

    CAO H Y, LI H, DUAN Q S, et al. Research progress on extraction technology of rice bran protein[J]. The Food Industry, 2021, 42(7): 280-283.
    [14]
    张琴秋, 秦文, 胡欣洁. 米糠蛋白提取和利用的研究进展[J]. 食品与发酵工业,2021,47(19):307−313. [ZHANG Q Q, QIN W, HU X J. Research progress on extraction and utilization of rice bran protein[J]. Food and Fermentation Industries,2021,47(19):307−313.

    ZHANG Q Q, QIN W, HU X J. Research progress on extraction and utilization of rice bran protein[J]. Food and Fermentation Industries, 2021, 47(19): 307-313.
    [15]
    王乔, 侯同刚, 咸漠, 等. 两步酶解提取米糠蛋白的工艺研究及优化[J]. 氨基酸和生物资源,2016,38(4):77−83. [WANG Q, HOU T G, JIAN M, et al. Study and optimization on the extraction of rice bran protein by Two-Step enzymatic hydrolysis[J]. Biotic Resources,2016,38(4):77−83. doi: 10.14188/j.ajsh.2016.04.014

    WANG Q, HOU T G, JIAN M, et al. Study and optimization on the extraction of rice bran protein by Two-Step enzymatic hydrolysis[J]. Biotic Resources, 2016, 38(4): 77-83. doi: 10.14188/j.ajsh.2016.04.014
    [16]
    HANSEN B B, SPITTLE S, CHEN B, et al. Deep eutectic solvents: A review of fundamentals and applications[J]. Chemical Reviews, 2020: 9-14.
    [17]
    MAGDALENA P, SASCHA S, SELIN K, et al. Deep eutectic solvents as efficient solvents in biocatalysis[J]. Trends in Biotechnology,2019,37(9):943−959. doi: 10.1016/j.tibtech.2019.03.007
    [18]
    SVIGELJ R, DOSSI N, GRAZIOLI C, et al. Deep eutectic solvents (DESs) and their application in biosensor development[J]. Sensors (Basel, Switzerland),2021,21(13):4263−4263. doi: 10.3390/s21134263
    [19]
    POPOVIC B M, MICIC N, POTKONJAK A, et al. Novel extraction of polyphenols from sour cherry pomace using natural deep eutectic solvents-ultrafast microwave-assisted NADES preparation and extraction[J]. Food Chemistry,2022,366:130562. doi: 10.1016/j.foodchem.2021.130562
    [20]
    ZANNOU O, PASHAZADEH H, IBRAHIM S A, et al. Green and highly extraction of phenolic compounds and antioxidant capacity from kinkeliba (Combretum micranthum G. Don) by natural deep eutectic solvents (NADESs) using maceration, ultrasound-assisted extraction and homogenate-assisted extraction[J]. Arabian Journal of Chemistry,2022,15(5):1−14.
    [21]
    ZULLAIKAH S, WIBOWO N D, WAHYUDI I M G E, et al. Deacidification of crude rice bran oil (CRBO) to remove FFA and preserve γ-oryzanol using deep eutectic solvents (DES)[J]. Materials Science Forum,2019,964:109−114. doi: 10.4028/www.scientific.net/MSF.964.109
    [22]
    MULIA K, NASIKIN M, KRISANTI E A, et al. Deacidification of palm oil using betaine monohydrate-carboxylic acid deep eutectic solvents: Combined extraction and simple solvent recovery[J]. Processes,2020,8(5):543−543. doi: 10.3390/pr8050543
    [23]
    LIN J J, XIANG H, D, SUN D X, et al. Deep eutectic solvents and alkaline extraction of protein from seabuckthorn seed meal: A comparison study[J]. Food Science and Human Wellness,2022,11(4):1028−1035. doi: 10.1016/j.fshw.2022.03.019
    [24]
    ESTER H C, NADIA B, MARÍA L M, et al. High voltage electrical discharges followed by deep eutectic solvents extraction for the valorization of pomegranate seeds (Punica granatum L.)[J]. Innovative Food Science and Emerging Technologies,2022,79:103055−103055. doi: 10.1016/j.ifset.2022.103055
    [25]
    PANG J Y, SHA X F, CHAO Y T, et al. Green aqueous biphasic systems containing deep eutectic solvents and sodium salts for the extraction of protein[J]. RSC Advances,2017,7(78):49361−49367. doi: 10.1039/C7RA07315A
    [26]
    ZhOU Y H, WU W, ZHANG N, et al. Deep eutectic solvents as new media for green extraction of food proteins: Opportunity and challenges[J]. Food Research International,2022,161:111842−11842. doi: 10.1016/j.foodres.2022.111842
    [27]
    ZHOU P F, LI J B, YAN T, et al. Selectivity of deproteinization and demineralization using natural deep eutectic solvents for production of insect chitin (Hermetia illucens)[J]. Carbohydrate Polymers,2019,225:115255−115256. doi: 10.1016/j.carbpol.2019.115255
    [28]
    李淑婷, 邓媛元, 魏振承, 等. 天然低共熔溶剂提取米糠酯酶工艺优化及其纯化与酶学性质研究[J]. 食品工业科技,2023,44(6):210−219. doi: 10.13386/j.issn1002-0306.2022050332
    [29]
    BRADOFRD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry,1976,72(1−2):248−254. doi: 10.1016/0003-2697(76)90527-3
    [30]
    ZhOU P, WANG X, YANG B, et al. Chemoenzymatic epoxidation of alkenes with Candida antarctica lipase B and hydrogen peroxide in deep eutectic solvents[J]. RSC Advances,2017,7(21):12518−12523. doi: 10.1039/C7RA00805H
    [31]
    LIU X, WANG M, ZHANG X, et al. The correlation between the physicochemical properties of water-based deep eutectic solvents and catalytic activity of lipase Novozym 435[J]. Journal of Molecular Liquids, 2020: 115200-115200.
    [32]
    YUN T, GEERT-JAN W, ROBRET V, YOUNG H. Tailoring properties of natural deep eutectic solvents with water to facilitate their applications[J]. Food Chemistry, 2015, 187−188.
    [33]
    SHI L, ZHENG L, ZHAO C, et al. Effects of deacidification methods on high free fatty acid containing oils obtained from sea buckthron (Hippophaër hamnoides L.) berry[J]. Industrial Crops and Products,2018,124:797−805. doi: 10.1016/j.indcrop.2018.08.059
  • Cited by

    Periodical cited type(9)

    1. 袁求松,张玉,付湘晋,向延菊. 市售22种梅干菜滋味品质与理化指标相关性分析. 食品安全质量检测学报. 2025(06): 316-322 .
    2. 刘仁杰,卞方圆,黄志远,伊奎鑫. 添加毛竹笋混合发酵对梅干菜主要功能性物质及营养成分变化的影响. 竹子学报. 2024(01): 11-18 .
    3. 李珊慧,林登峰,赵洪雷,李学鹏,励建荣,徐永霞. 双响应值联合优化超声波辅助熬制雪菜大黄鱼汤工艺及其滋味评价. 中国调味品. 2024(06): 43-49+76 .
    4. 李艺,童秀子,徐伟程,郭斯统,陈育如,郭宇星,孔晓雪,罗海波. 宁波地区传统梅干菜中优势发酵菌株分离鉴定及酶活分析. 食品工业科技. 2023(03): 154-162 . 本站查看
    5. 史学敏,刘珊娜,杜茜. 鄂尔多斯地区酸粥细菌多样性分析及乳酸菌的分离鉴定. 食品工业科技. 2023(09): 153-159 . 本站查看
    6. 任锡亮,高天一,莫旺成,王洁,孟秋峰. 不同盐度和腌制时间对雪菜的影响. 中国果菜. 2023(10): 25-28 .
    7. 田丽君,荊永锋,郑卜凡,彭新辉,龚嘉蕾,吴涛,李正风,李振杰,田祥珅,周启运,龚嘉,黎娟. 浏阳洞库陈化烟叶微生物多样性分析. 中国烟草学报. 2022(01): 98-107 .
    8. 庞春霞,李艺,虞任莹,甘甜,郭斯统,陈育如,罗海波. 基于Illumina Miseq技术比较不同地区传统发酵大豆制品细菌多样性. 食品工业科技. 2022(08): 133-140 . 本站查看
    9. 赵野,刘艳,周晓洁,钱鑫,张家铭,周文化. 腌制技术对芥菜品质影响的研究进展. 粮食与油脂. 2022(09): 22-26 .

    Other cited types(0)

Catalog

    Article Metrics

    Article views (196) PDF downloads (34) Cited by(9)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return