Effect of Different Processing Methods on the Quality Indexes and Enzyme Activities of <i>Rosa</i> Crimson Glory Tea and Correlation Analysis

ZHENG Xinyan; YI Runmei; XU Dongyue; ZUO Weigang; LI Yuchuan; LI Yanqiong; LIU He

doi:10.13386/j.issn1002-0306.2022090328

Volume 44 Issue 15

Jul. 2023

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Science and Technology of Food Industry > 2023 > 44(15): 337-345. > DOI: 10.13386/j.issn1002-0306.2022090328

ZHENG Xinyan, YI Runmei, XU Dongyue, et al. Effect of Different Processing Methods on the Quality Indexes and Enzyme Activities of Rosa Crimson Glory Tea and Correlation Analysis[J]. Science and Technology of Food Industry, 2023, 44(15): 337−345. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090328.

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Effect of Different Processing Methods on the Quality Indexes and Enzyme Activities of Rosa Crimson Glory Tea and Correlation Analysis

1.
College of Agricultural and Life Science, Kunming University, Kunming 650214, China
2.
College of Agricultural, Honghe Vocational and Technical College, Honghe 661199, China

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Received Date: October 07, 2022
Available Online: June 05, 2023

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Abstract

Abstract

At present, there are some problems in rose tea on the market, such as single processing method, large loss of aroma after brewing, etc. In this study, with reference to tea processing, fresh rose (Rosa crimson glory) petals were processed into scented tea by four methods: Room temperature-drying, withering-fermentation-drying, fixation-drying and fixation-fermentation-drying respectively. The quality indexes of scented tea such as sensory quality, aroma composition, and content of active substances (total phenols, total flavonoids, and anthocyanins) were compared and analyzed. In addition, the enzymatic activities of polyphenol oxidase and peroxidase in the scented tea were determined. Besides, the correlations between the quality indexes and the activities of the two enzymes were analyzed. The results showed that the total sensory evaluation score of rose tea processed by fixation-fermentation-drying treatment was 75.00±8.30, significantly higher than that of rose tea processed by other methods (P<0.05) and it performed better in all factors of sensory quality. The content of the main aroma components, such as citronellol content, in thr rose tea produced by the fixation-drying and fixation-fermentation-drying methods were 20838.61±63.06 and 28218.71±14.95 μg/kg respectively, which was significantly higher than those obtained by withering-fermentation and room-temperature drying methods (P<0.05). In addition, the content of total phenols (184.17±2.04 mg GAE/100 g DW) and flavonoids (92.63±2.48 mg rutin equivalent/100 g DW) in rose tea processed by fixation-fermentation-drying method was significantly increased compared to other processing methods (P<0.05). However, it was unfavorable to the retention of anthocyanins when processed by withering-fermentation-drying, fixation-drying and fixation-fermentation-drying methods. The correlation between the quality indexes and the enzyme activities of the two enzymes showed that there was a negative correlation between the peroxidase enzyme activity and the aroma components of esters, aldehydes, alcohols, acids and alkanes, while there was a positive correlation between the polyphenol oxidase enzyme and the aldehyde aroma components (r=0.602). There was a negative correlation between the polyphenol oxidase enzyme activity and the content of active substances as total phenols, total flavonoids, anthocyanins, while the peroxidase enzyme was negatively correlated with the content of anthocyanins (r=0.202). The enzyme activity of the two enzymes, especially polyphenol oxygenase activity, could be effectively inhibited by fixation processing. In general, the rose tea produced by the method of fixation possessed a higher score and higher main aroma component contents. The rose tea produced by the method of fixation-fermentation-drying method had the highest content of total phenols and flavonoids. The correlation analysis between the enzyme activities and the quality indexes of rose tea showed that the quality of rose tea could be improved by regulating the enzyme activities through processing methods.
- withering,
- fixation,
- rose tea,
- aroma,
- active substances contents

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References

[1]	中国科学院植物志委员会. 中国植物志[M]. 北京: 科学出版社, 1985 Flora Committee of Chinese Academy of Sciences. Flora of China[M]. Beijing: Science Press, 1985.
[2]	宁忻, 方伟, 董海燕, 等. 云南墨红玫瑰主要营养成分分析[J]. 现代食品,2021,18:226−228. [NING X, FANG W, DONG H Y, et al. Analysis and evaluation of the nutritional components in Rosa Crimson Glory[J]. Modern Food,2021,18:226−228. NING X, FANG W, DONG H Y, et al. Analysis and evaluation of the nutritional components in Rosa Crimson Glory[J]. Modern Food, 2021, 18: 226-228.
[3]	刘真, 汤晓娟, 王彬, 等. 基于风味成分分析的不同干燥方式玫瑰花茶鉴别技术研究[J]. 食品安全质量检测学报,2022,13(12):3842−3849. [LIU Z, TANG X J, WANG B, et al. Study on identification technology of rose tea with different drying methods based on flavor compounds analysis[J]. Journal of Food Safety and Quality,2022,13(12):3842−3849. LIU Z, TANG X J, WANG B, et al. Study on identification technology of rose tea with different drying methods based on flavor compounds analysis[J]. Journal of Food Safety and Quality, 2022, 13(12): 3842-3849.
[4]	王海燕, 吴普侠, 王海鹰, 等. 大马士革有机玫瑰红茶的窨制研究[J]. 中国农业文摘-农业工程,2021,33(2):33−35. [WANG H Y, WU P X, WANG H Y, et al. Research on the cellaring of Damascus organic rose black tea[J]. China Agricultural Digest-Agricultural Engineering,2021,33(2):33−35. WANG H Y, WU P X, WANG H Y, et al. Research on the cellaring of Damascus organic rose black tea[J]. China Agricultural Digest-Agricultural Engineering, 2021, 33(2): 33-35.
[5]	李晓哲, 杨春花, 林琳, 等. 微波消解-ICP-MS法测定5种玫瑰花茶矿物质元素含量及溶出度的研究[J]. 食品科技,2020,45(11):269−274. [LI X Z, YANG C H, LIN L, et al. Determination of minerals and dissolution of five rose tea by microwave digestion and ICP-MS[J]. Food Science and Technology,2020,45(11):269−274. LI X Z, YANG C H, LIN L, et al. Determination of minerals and dissolution of five rose tea by microwave digestion and ICP-MS[J]. Food Science and Technology, 2020, 45(11): 269-274.
[6]	郝瑞杰, 程福慧, 杨姝婷. 干制对玫瑰花芳香品质的影响研究[J]. 西北林学院学报,2022,37(2):127−132. [HAO R J, CHENG F H, YANG S T, et al. Effect of drying on the aroma quality of Rosa rugose flowers[J]. Journal of Northwest Forestry University,2022,37(2):127−132. HAO R J, CHENG F H, YANG S T, et al. Effect of drying on the aroma quality of Rosa rugose flowers[J]. Journal of Northwest Forestry University, 2022, 37(2): 127-132.
[7]	KUMAR A, NADDA G, SHANKER A. Determination of chlorpyrifos 20% EC (Dursban 20 EC) in scented rose and its products[J]. Journal of Chromatography A,2004,1050(2):193−199.
[8]	张莲莉, 戴伟锋, 覃豪, 等. 三种玫瑰花茶中黄酮类成分的分析及其抗氧化活性研究[J]. 昆明理工大学学报(自然科学版),2019,44(5):84−90. [ZHANG L L, DAI W F, QIN H, et al. Flavonoids content analysis of three rose tea infusions and their antioxidant activities[J]. Journal of Kunming University of Science and Technology (Natural Science),2019,44(5):84−90. ZHANG L L, DAI W F, QIN H, et al. Flavonoids content analysis of three rose tea infusions and their antioxidant activities[J]. Journal of Kunming University of Science and Technology (Natural Science), 2019, 44(5): 84-90.
[9]	丁健. 茶叶加工过程对咖啡叶化学成分和生物活性影响[D]. 镇江: 江苏大学, 2021 DING J. Study on the effect of tea processing on the chemical components and bioactivities in coffee leaves[D]. Zhenjiang: Jiangsu University, 2021.
[10]	施兆鹏. 茶叶审评与检验(第四版)[M]. 北京: 中国农业出版社, 2010: 141−146 SHI Z P. Tea qualification and inspection (Fourth edition)[M]. Beijing: China Agricultural Press, 2010: 141−146.
[11]	ZHAO C Y, XUE J, CAI X D, et al. Assessment of the key aroma compounds in rose-based products[J]. Journal of Food and Drug Analysis,2016,24:471−476. doi: 10.1016/j.jfda.2016.02.013
[12]	熊汝琴, 李平, 王锐. Folin-Ciocalteu比色法测定昭通青花椒总酚含量[J]. 中国调味品,2019,44(10):140−143. [XIONG R Q, LI P, WANG R. Determination of total phenols in Zhaotong zanthoxylum schinifolium by Folin-Ciocalteu colorimetry[J]. China Condiment,2019,44(10):140−143. XIONG R Q, LI P, WANG R. Determination of total phenols in Zhaotong zanthoxylum schinifolium by Folin-Ciocalteu colorimetry[J]. China Condiment, 2019, 44(10): 140-143.
[13]	JIA Z S, TANG M C, WU J M. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals[J]. Food Chemistry,1999,64(4):555−559. doi: 10.1016/S0308-8146(98)00102-2
[14]	樊婷, 何建军, 陈学玲, 等. 柠檬酸—乙醇法提取紫甘薯花青素工艺的优化[J]. 湖北农业科学,2012,51(23):5448−5451. [FAN T, HE J J, CHEN X L, et al. Optimization of extraction technology of anthocanin from purple sweet potato by citric acid-ethanol method[J]. Hubei Agricultural Sciences,2012,51(23):5448−5451. doi: 10.3969/j.issn.0439-8114.2012.23.057 FAN T, HE J J, CHEN X L, et al. Optimization of extraction technology of anthocanin from purple sweet potato by citric acid-ethanol method[J]. Hubei Agricultural Sciences, 2012, 51(23): 5448-5451. doi: 10.3969/j.issn.0439-8114.2012.23.057
[15]	吴咏, 金建, 付秀丽, 等. 超声波、紫外线、超声波联合紫外线对鲜切香菇多酚氧化酶与过氧化物酶的钝化及其机理研究[J]. 食品工业科技,2020,41(20):20−25, 32. [WU Y, JIN J, FU X L, et al. Effects of ultrasound, ultraviolet, ultrasound synchronized ultraviolet on the inactivation of polyphenol oxidase and peroxidase of fresh-cut Lentinus edodes and its mechanisms[J]. Science and Technology of Food Industry,2020,41(20):20−25, 32. WU Y, JIN J, FU X L, et al. Effects of ultrasound, ultraviolet, ultrasound synchronized ultraviolet on the inactivation of polyphenol oxidase and peroxidase of fresh-cut Lentinus edodes and its mechanisms[J]. Science and Technology of Food Industry, 2020, 41(20): 20-25, 32.
[16]	GONG Z, OUYANG J, WU X, et al. Dark tea extracts: Chemical constituents and modulatory effect on gastrointestinal function[J]. Biomedicine & Pharmacotherapy,2020,130:110514.
[17]	YAO Y, WU M, HUANG Y, et al. Appropriately raising fermentation temperature beneficial to the increase of antioxidant activity and gallic acid content inEurotium cristatum-fermented loose tea[J]. Food Science & Technology,2017,82:248−254.
[18]	潘顺顺, 李家贤, 孙世利, 等. 特色品种绿茶成分与茶汤色泽的相关性研究[J]. 华南农业大学学报,2012,33(3):307−310. [PAN S S, LI J X, SUN S L, er al. Study on correlation between contituents and liquor color of a distinctive green tea[J]. Journal of South China Agricultural University,2012,33(3):307−310. doi: 10.7671/j.issn.1001-411X.2012.03.007 PAN S S, LI J X, SUN S L, er al. Study on correlation between contituents and liquor color of a distinctive green tea[J]. Journal of South China Agricultural University, 2012, 33(3): 307-310. doi: 10.7671/j.issn.1001-411X.2012.03.007
[19]	刘金仙, 卢莉, 傅仙玉, 等. 奇兰秋季红茶加工过程主要生化成分变化及相关酶基因表达[J]. 热带作物学报,2022,43(11):2383−2393. [LIU J X, LU L, FU X Y, et al. Changes of main biochemical components and expression of related enzyme genes of oilan autumn black tea during processing[J]. Chinese Journal of Tropical Crops,2022,43(11):2383−2393. LIU J X, LU L, FU X Y, et al. Changes of main biochemical components and expression of related enzyme genes of oilan autumn black tea during processing[J]. Chinese Journal of Tropical Crops, 2022, 43(11): 2383-2393.
[20]	陈红艳, 廖蓉苏, 杨今朝, 等. 玫瑰花挥发性化学成分的分析研究[J]. 食品科技,2011,36(11):186−190,196. [CHEN H Y, LIAO R S, YANG J Z, et al. Analysis of volatile chemical compounds from the ﬂ ower of rosa rugosa[J]. Food Science and Technology,2011,36(11):186−190,196. CHEN H Y, LIAO R S, YANG J Z, et al. Analysis of volatile chemical compounds from the ﬂ ower of rosa rugosa[J]. Food Science and Technology, 2011, 36(11): 186-190, 196.
[21]	JIN J, WU N, ZHU L, et al. Determination of volatiles in Rosa Rugosa by knitting aromatic polymer-polydopamine (KAP@PDA) solid-phase microextraction (SPME) and multidimensional gas chromatography-mass spectrometry (MDGC-MS)[J]. Analytical Letters,2022,55(2):246−255. doi: 10.1080/00032719.2021.1922433
[22]	张玉玉, 宋弋, 李全宏. 食品中糠醛和5-羟甲基糠醛的产生机理、含量检测及安全性评价研究进展[J]. 食品科学,2021,33(5):275−280. [ZHANG Y Y, SONG Y, LI Q H. A Review on formation mechanism, determination and safety assessment of furfural and 5-hydroxymethylfurfural (HMF) in foods[J]. Food Science,2021,33(5):275−280. ZHANG Y Y, SONG Y, LI Q H. A Review on formation mechanism, determination and safety assessment of furfural and 5-hydroxymethylfurfural (HMF) in foods[J]. Food Science, 2021, 33(5): 275-280.
[23]	巩敏. 镇江香醋中糠醛的形成途径及调控研究[D]. 无锡: 江南大学, 2021 GONG M. Study on the formation pathways and control of furfural in Zhenjiang aromatic vinegar[D]. Wuxi: Jiangnan University, 2021.
[24]	苗潇潇. 玫瑰花露香气成分分析及其抑菌作用初探[D]. 太原: 山西大学, 2017 MIAO X X. Research on aroma components and antimicrobial activity of rose hydrolat[D]. Taiyuan: Shanxi University, 2017.
[25]	WANG X H, CAI L L. Navigation and control based on integral-uncertainty observer for unmanned jet aircraft[J]. IEEE Transactions on Aerospace and Electronic Systems,2017,53(3):1230−1249. doi: 10.1109/TAES.2017.2668078
[26]	QIN H, DENG X Q, LI B C, et al. Volatiles, polysaccharides and total polyphenols in Chinese rose tea infusions and their antioxidant activities[J]. Journal of Food Processing and Preservation,2017,42(1):e13323.
[27]	杨涵雨. 黄茶闷黄工序及微生物对黄茶品质的影响研究[D]. 长沙: 湖南农业大学, 2014 YANG H Y. Studies on the yellowing with piling process and the effect of microorganisms on the yellow tea[D]. Changsha: Hunan Agricultural University, 2014.
[28]	赵越. 加工工艺对不同品种绿茶苦涩味影响的研究[D]. 杭州: 浙江大学, 2015 ZHAO Y. Effect of processing on bitterness and astringency of green tea of various cultivars[D]. Hangzhou: Zhejiang University, 2015.
[29]	YU X, LI Y, HE C, et al. Nonvolatile metabolism in postharvest tea (Camellia sinensis L.) leaves: Effects of different withering treatments on nonvolatile metabolites, gene expression levels, and enzyme activity[J]. Food Chemistry,2020,327:126992. doi: 10.1016/j.foodchem.2020.126992
[30]	解东超. 紫娟茶中花青素及其在加工过程中变化规律研究[D]. 北京: 中国农业科学院, 2017 XIE D C. Research on anthocyanins of zijuan and their varying patterns during the process[D]. Beijing: Chinese Academy of Agricultural Sciences, 2017.
[31]	DARAVINGAS G, CAIN R F. Thermal degradation of black raspberry anthocyanin pigments in model systems[J]. Journal of Food Science,2006,33(2):138−142.
[32]	梅菊芬, 徐德良, 汤茶琴, 等. 茶树花青素及其种质资源的研究和利用进展[J]. 热带农业工程,2013,37(1):42−46. [MEI J F, XU D L, TANG C Q, et al. Advances on research and utilization of tea tree (Camellia sinensis) anthocyanins and Its germplasm resources[J]. Tropical Agricultural Engineering,2013,37(1):42−46. [MEI J F, XU D L, TANG C Q, et al. Advances on research and utilization of tea tree (Camellia sinensis) anthocyanins and Its germplasm resources [J]. Tropical Agricultural Engineering, 2013, 37(1): 42-46.
[33]	丰金玉, 刘昆言, 秦昱, 等. 红茶加工中多酚氧化酶、过氧化物酶和β-葡萄糖苷酶活性变化[J]. 农学学报,2014,4(11):96−99. [FENG J Y, LIU K Y, QIN Y, et al. Activity changes of polyphenol oxidase, peroxidase and β-glycosidase in black tea processing[J]. Journal of Agriculture,2014,4(11):96−99. FENG J Y, LIU K Y, QIN Y, et al. Activity changes of polyphenol oxidase, peroxidase and β-glycosidase in black tea processing[J]. Journal of Agriculture, 2014, 4(11): 96-99.
[34]	VILLARREAL-SOTO S A, BEAUFORT S, BOUAJILA J, et al. Impact of fermentation conditions on the production of bioactive compounds with anticancer, anti-inflammatory and antioxidant properties in kombucha tea extracts[J]. Process Biochemistry,2019,83:44−54. doi: 10.1016/j.procbio.2019.05.004
[35]	谭和平, 周李华, 钱杉杉, 等. 茶叶发酵中的酶学研究进展[J]. 中国测试,2009,35(1):19−23. [TAN H P, ZHOU L H, QIAN S S, et al. Research progress of enzyme in tea fermentation[J]. China Measurement & Test,2009,35(1):19−23. TAN H P, ZHOU L H, QIAN S S, er al. Research progress of enzyme in tea fermentation[J]. China Measurement & Test, 2009, 35(1): 19-23.
[36]	林珠稻, 陈韵杨. 不同萎凋方式对福鼎白茶品质的影响[J]. 福建茶业,2022,3:20−24. [LIN Z T, CHEN Y Y. Effect of different withering methods on the quality of Fuding white tea[J]. Fujian Tea Industry,2022,3:20−24. LIN Z T, CHEN Y Y. Effect of different withering methods on the quality of Fuding white tea[J]. Fujian Tea Industry, 2022, 3: 20-24.
[37]	汪蓓. 杀青与提香温度对绿茶香型及其特征香气影响的研究[D]. 重庆: 西南大学, 2020 WANG B. Study on the effects of pan-firing and baking temperature on the aroma type and characteristic of green tea[D]. Chongqing: Southwest University, 2020.

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