WANG Rongrong, QIAN Yilin, TANG Zhixin, et al. Physicochemical Characterization and in Vivo Hypolipidemic Effect of Chitosan-Coated Nuciferine Liposomes[J]. Science and Technology of Food Industry, 2023, 44(7): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090226.
Citation: WANG Rongrong, QIAN Yilin, TANG Zhixin, et al. Physicochemical Characterization and in Vivo Hypolipidemic Effect of Chitosan-Coated Nuciferine Liposomes[J]. Science and Technology of Food Industry, 2023, 44(7): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090226.

Physicochemical Characterization and in Vivo Hypolipidemic Effect of Chitosan-Coated Nuciferine Liposomes

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  • Received Date: September 21, 2022
  • Available Online: February 03, 2023
  • Objective: Chitosan-coated nuciferine liposomes (CS-LP) were prepared and their hypolipidemic effects in vivo were investigated. Methods: The CS-LP were prepared by thin film dispersion and pH gradient methods, and their particle size and potential distribution, encapsulation rate, microscopic morphology and in vitro release curves were determined, and their hypolipidemic effects on obese mice were investigated. Results: The CS-LP was produced in a homogeneous spherical structure with a particle size of 253.54±4.25 nm, a zeta potential of 32.50±3.44 mV. The encapsulation rate of CS-LP was 83.46%. Compared with nuciferine liposomes (LP), the stability of CS-LP in the simulated digestive solution was improved after adding the chitosan. Compared with the high-fat group, mice gavaged with CS-LP for 11 weeks showed a 12.91% and 41.03% significant reduction in body weight and adipose tissue weight, respectively (P<0.05), a 32.40% and 14.49% significant reduction in serum triglyceride and LDL cholesterol mass concentrations, respectively (P<0.05), and a normalization of lipid levels and a reduction in adipocyte volume. High-throughput sequencing analysis showed that CS-LP significantly increased the diversity of intestinal flora in mice (P<0.05). At the phylum level, the relative abundance of the thick-walled phylum and the relative abundance of the bacillus phylum increased significantly, and the ratio of the campylobacter and thick-walled phylum to the relative abundance of the mimic phylum also significantly decreased (P<0.05). Conclusion: The chitosan-coated nuciferine liposomes with high encapsulation rate and slow release could effectively improve obesity and intestinal flora disorders in mice caused by high-fat diet, and express the broad application prospects in the development of lipid-lowering formulations.
  • [1]
    ZHENG H, HAN L, SHI W, et al. Research advances in lotus leaf as chinese dietary herbal medicine[J]. The American Journal of Chinese Medicine,2022,50(6):1423−1445. doi: 10.1142/S0192415X22500616
    [2]
    YU Y, LU J, SUN L, et al. Akkermansia muciniphila: A potential novel mechanism of nuciferine to improve hyperlipidemia[J]. Biomedicine & Pharmacotherapy,2021:133.
    [3]
    ZHANG L N, GAO J H, TANG P, et al. Nuciferine inhibits LPS-induced inflammatory response in BV2 cells by activating PPAR-gamma[J]. International Immunopharmacology,2018,63:9−13. doi: 10.1016/j.intimp.2018.07.015
    [4]
    QI Q, LI R, LI H Y, et al. Identification of the anti-tumor activity and mechanisms of nuciferine through a network pharmacology approach[J]. Acta Pharmacologica Sinica,2016,37(7):963−972. doi: 10.1038/aps.2016.53
    [5]
    CHEN H W, YANG M Y, HUNG T W, et al. Nelumbo nucifera leaves extract attenuate the pathological progression of diabetic nephropathy in high-fat diet-fed and streptozotocin-induced diabetic rats[J]. Journal of Food and Drug Analysis,2019,27(3):736−748. doi: 10.1016/j.jfda.2018.12.009
    [6]
    MA C, LI G, HE Y, et al. Pronuciferine and nuciferine inhibit lipogenesis in 3T3-L1 adipocytes by activating the AMPK signaling pathway[J]. Life Sciences,2015,136:120−125. doi: 10.1016/j.lfs.2015.07.001
    [7]
    XU H, LU X, GUO X, et al. Distinct AMPK-mediated FAS/HSL pathway is implicated in the alleviating effect of nuciferine on obesity and hepatic steatosis in hfd-fed mice[J]. Nutrients,2022,14(9):25−46.
    [8]
    PAN S Z. Study on effect of alkaloid salt from lotus leaf on preventing of diet-induced obesity and modulation of gut microbiota in mice [D]. Zhejiang: Zhejiang University, 2019.
    [9]
    WANG Y X, LIU B, SHI R B, et al. Determination of nuciferine in Beagle dog by RP-HPLC and research on its pharmacokinetics[J]. Chinese Journal of Pharmaceutical Analysis,2008,28(9):1418−1421.
    [10]
    WANG F G, CAO J, HOU X Q, et al. Pharmacokinetics, tissue distribution, bioavailability, and excretion of nuciferine, an alkaloid from lotus, in rats by LC/MS/MS[J]. Drug Development and Industrial Pharmacy,2018,44(9):1557−1562. doi: 10.1080/03639045.2018.1483399
    [11]
    XIE Y L, WANG Y Y, TAN Y X, et al. Study on absolute bioavailability of nuciferine in rats[J]. Journal of Guangdong Pharmaceutical University,2010,26(01):17−19.
    [12]
    COLLIER M A, BACHELDER E M, AINSLIE K M. Electrosprayed myocet-like liposomes: An alternative to traditional liposome production[J]. Pharmaceutical Research,2017,34(2):419−426. doi: 10.1007/s11095-016-2072-4
    [13]
    SEBAALY C, HAYDAR S, GREIGE-GERGES H. Eugenol encapsulation into conventional liposomes and chitosan-coated liposomes: A comparative study[J]. Journal of Drug Delivery Science and Technology,2022:67.
    [14]
    HE H S, LU Y, QI J P, et al. Adapting liposomes for oral drug delivery[J]. Acta Pharmaceutica Sinica B,2019,9(1):36−48. doi: 10.1016/j.apsb.2018.06.005
    [15]
    TAI K D, RAPPOLT M, MAO L K, et al. The stabilization and release performances of curcumin-loaded liposomes coated by high and low molecular weight chitosan[J]. Food Hydrocolloids,2020:99.
    [16]
    TIAN M, HAN J, YE A, et al. Structural characterization and biological fate of lactoferrin-loaded liposomes during simulated infant digestion[J]. Journal of the Science of Food and Agriculture,2019,99(6):2677−2684. doi: 10.1002/jsfa.9435
    [17]
    WANG W, TAN X M. Study on the determination of total alkaloid content of lotus leaves[J]. Journal of Chinese Medicinal Materials,2004(1):50−51.
    [18]
    BONECHI C, TAMASI G, DONATI A, et al. Physicochemical characterization of hyaluronic acid and chitosan liposome coatings[J]. Applied Sciences-Basel, 2021, 11(24).
    [19]
    EFIMOVA A A, MULASHKIN F D, RUDENSKAYA G N, et al. Biodegradable electrostatic complexes of chitosan cationic microparticles and anionic liposomes[J]. Polymer Science, Series B,2018,60(1):84−90. doi: 10.1134/S1560090418010037
    [20]
    MANCONI M, MANCA M L, VALENTI D, et al. Chitosan and hyaluronan coated liposomes for pulmonary administration of curcumin[J]. International Journal of Pharmaceutics,2017,525(1):203−210. doi: 10.1016/j.ijpharm.2017.04.044
    [21]
    KHOERUNNISA F, NURHAYATI M, DARA F, et al. Physicochemical properties of tpp-crosslinked chitosan nanoparticles as potential antibacterial agents[J]. Fibers and Polymers,2021,22(11):2954−2964. doi: 10.1007/s12221-021-0397-z
    [22]
    LIU W L, HOU Y Y, JIN Y Y, et al. Research progress on liposomes: Application in food, digestion behavior and absorption mechanism[J]. Trends in Food Science & Technology,2020,104:177−189.
    [23]
    WANG Y, ZHANG W, REIMER B, et al. The effect of feedback on attitudes toward cellular phone use while driving: A comparison between novice and experienced drivers[J]. Traffic Injury Prevention,2010,11(5):471−477. doi: 10.1080/15389588.2010.495761
    [24]
    CHO Y A, KIM J. Effect of probiotics on blood lipid concentrations a meta-analysis of randomized controlled trials[J]. Medicine,2015,94(43):6371−6384.
    [25]
    BACKHED F, MANCHESTER J K, SEMENKOVICH C F, et al. Mechanisms underlying the resistance to diet-induced obesity in germ-free mice[J]. Proceedings of the National Academy of Sciences of the United States of America,2007,104(3):979−984. doi: 10.1073/pnas.0605374104
    [26]
    CURAT C A, MIRANVILLE A, SENGENÈS C, et al. From blood monocytes to adipose tissue-resident macrophages: Induction of diapedesis by human mature adipocytes[J]. Diabetes,2004,53(5):1285−1292. doi: 10.2337/diabetes.53.5.1285
    [27]
    BALLANTYNE C M, OLSSON A G, COOK T J, et al. Influence of low high-density lipoprotein cholesterol and elevated triglyceride on coronary heart disease events and response to simvastatin therapy in 4S[J]. Circulation,2001,104(25):3046−3051. doi: 10.1161/hc5001.100624
    [28]
    SMITH B J, MILLER R A, SCHMIDT T M. Muribaculaceae genomes assembled from metagenomes suggest genetic drivers of differential response to acarbose treatment in mice[J]. Msphere,2021,6(6):78−92.
    [29]
    SCHOELER M, CAESAR R. Dietary lipids, gut microbiota and lipid metabolism[J]. Reviews in Endocrine and Metabolic Disorders,2019,20(4):461−472. doi: 10.1007/s11154-019-09512-0
    [30]
    JUST S, MONDOT S, ECKER J, et al. The gut microbiota drives the impact of bile acids and fat source in diet on mouse metabolism[J]. Microbiome,2018,6(1):134. doi: 10.1186/s40168-018-0510-8
    [31]
    GAO X, JIA R, XIE L, et al. A study of the correlation between obesity and intestinal flora in school-age children[J]. Scientific Reports,2018,8(1):14511. doi: 10.1038/s41598-018-32730-6
    [32]
    TURNBAUGH P J, LEY R E, MAHOWALD M A, et al. An obesity-associated gut microbiome with increased capacity for energy harvest[J]. Nature,2006,444(7122):1027−1031. doi: 10.1038/nature05414
    [33]
    LI Y, LI N, LIU J, et al. Gegen qinlian decoction alleviates experimental colitis and concurrent lung inflammation by inhibiting the recruitment of inflammatory myeloid cells and restoring microbial balance[J]. J Inflamm Res,2022,15:1273−1291. doi: 10.2147/JIR.S352706

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