Morphological Characterization and Identification of Five Pathogenic Fungi from Apricot Fruit during Postharvest Storage

WANG Ke; YAO Ting; ZHANG Yan; HE Xinmeng; WANG Yousheng

doi:10.13386/j.issn1002-0306.2022090204

Volume 44 Issue 17

Aug. 2023

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Science and Technology of Food Industry > 2023 > 44(17): 121-128. > DOI: 10.13386/j.issn1002-0306.2022090204

WANG Ke, YAO Ting, ZHANG Yan, et al. Morphological Characterization and Identification of Five Pathogenic Fungi from Apricot Fruit during Postharvest Storage[J]. Science and Technology of Food Industry, 2023, 44(17): 121−128. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090204.

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Morphological Characterization and Identification of Five Pathogenic Fungi from Apricot Fruit during Postharvest Storage

WANG Ke^{1, 2,},
YAO Ting^{1, 2},
ZHANG Yan¹,
HE Xinmeng¹,
WANG Yousheng^{1, 2, ,}

1.
Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Technology & Business University (BTBU), Beijing 100048, China
2.
Rizhao HUAWEI Institute of Comprehensive Health Industries, Shandong KEEPFIT Biotech Co., Ltd., Rizhao 276801, China

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Received Date: September 21, 2022
Available Online: July 03, 2023

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Abstract

Abstract

Apricot fruit is highly susceptible to infection by pathogenic fungi. In this study, the pathogenic fungi in the diseased parts of the fruits of ‘Golden Sun’ and ‘Red almond’ apricot (Prunus armeniaca L.) during postharvest storage were isolated and purified, and the purified strains were inoculated back to the healthy fruits of corresponding varieties, showing the same disease symptoms as the diseased fruits. Five fungi isolates were identified based on morphological characteristics and internal transcribed spacer (ITS) sequence analysis combined with construction of phylogenetic tree. Three isolates from ‘Golden Sun’ apricot were identified as Diaporthe eres (325^#), Botryosphaeria dothidea (327^#) and Aureobasidium pullulans (328^#). Two isolates from ‘Red almond’ apricot were identified as Botrytis elliptica (326^#) and Botrytis cinerea (331^#). D. eres, B. dothidea, A. pullulans and B. elliptica were pathogenic fungi that had not been reported in the literature on apricot fruits, whereas B. cinerea had not been reported on the infection of ‘Red almond’ apricot. The present results provided references for the biological control measures of apricot fruits.
- apricot fruit,
- postharvest diseases,
- pathogenic fungi,
- rDNA ITS sequence analysis

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References

[1]	夏乐晗, 陈玉玲, 冯义彬, 等. 不同品种杏果实发育过程中类黄酮、总酚和三萜酸含量及抗氧化性研究[J]. 果树学报,2016,33(4):425−435. [XIA L H, CHEN Y L, FENG Y B, et al. Changes in flavonoids, total phenolics, triterpenoidic acids and antioxidant capacity during fruit development of different cultivars of apricot[J]. Journal of Fruit Science,2016,33(4):425−435. doi: 10.13925/j.cnki.gsxb.20150395 XIA L H, CHEN Y L, FENG Y B, et al. Changes in flavonoids, total phenolics, triterpenoidic acids and antioxidant capacity during fruit development of different cultivars of apricot[J]. Journal of Fruit Science, 2016, 33(4): 425-435. doi: 10.13925/j.cnki.gsxb.20150395
[2]	张俊环, 杨丽, 孙浩元, 等. 不同品种杏果实发育进程中多酚与类黄酮物质含量的变化[J]. 北方园艺,2012,24:1−5. [ZHANG J H, YANG L, SUN H Y, et al. Changes on the total phenols and flavonoids in apricot peel and pulp of different cultivars during fruit development[J]. Northern Horticulture,2012,24:1−5. ZHANG J H, YANG L, SUN H Y, et al. Changes on the total phenols and flavonoids in apricot peel and pulp of different cultivars during fruit development[J]. Northern Horticulture, 2012, 24: 1-5
[3]	张瑾, 徐秉良, 梁巧兰, 等. 杏采后病害病原菌鉴定及室内药剂筛选[J]. 植物保护,2011,37(5):118−123. [ZHANG J, XU B L, LIANG Q L, et al. Identification of the pathogens of postharvest apricot fruit diseases and indoor screening of fungicides[J]. Plant Protection,2011,37(5):118−123. ZHANG J, XU B L, LIANG Q L, et al. Identification of the pathogens of postharvest apricot fruit diseases and indoor screening of fungicides[J]. Plant Protection, 2011, 37(5): 118-123.
[4]	阿衣木古丽·艾赛提. 杏采后病原菌鉴定及采前壳寡糖和水杨酸处理对杏品质和病害的影响[D]. 乌鲁木齐: 新疆农业大学, 2014: 1−30. AYIMUGULI A S T. Identification of the pathogenic fungus from post-harvest apricot fruit and effect of preharvest oligochitosan, salicylic acid treatment on storage quality and diseases of apricot fruit[D]. Urumqi: Xinjiang Agricultural University, 2014.
[5]	郑琪, 徐秉良, 薛应钰, 等. 杏采后病害病原拮抗菌的分离筛选及鉴定[J]. 植物保护,2013,39(4):34−39, 71. [ZHENG Q, XU B L, QUE Y Y, et al. Isolation, screening and identification of antagonistic strains against apricot postharvest diseases[J]. Plant Protection,2013,39(4):34−39, 71. doi: 10.3969/j.issn.0529-1542.2013.04.008 ZHENG Q, XU B L, QUE Y Y, et al. Isolation, screening and identification of antagonistic strains against apricot postharvest diseases[J]. Plant Protection, 2013, 39(4): 34-39, 71. doi: 10.3969/j.issn.0529-1542.2013.04.008
[6]	任向峰, 姚婷, 张萌, 等. 4株水果采后链格孢属真菌的rDNA ITS区序列与碳源代谢指纹图谱差异性分析[J]. 食品科学技术学报,2018,36(1):72−78, 94. [REN X F, YAO T, ZHANG M. Comparison of rDNA ITS Sequence andcarbon metabolic fingerprinting of four Alternaria Nees isolated from fruits[J]. Journal of Food Science and Technology,2018,36(1):72−78, 94. REN X F, YAO T, ZHANG M, Comparison of rDNA ITS Sequence andcarbon metabolic fingerprinting of four Alternaria Nees isolated from fruits [J]. Journal of Food Science and Technology, 2018, 36(1): 72-78, 94.
[7]	韩盛, 玉山江·麦麦提, 潘俨, 等. 鲜杏采后病原菌鉴定及室内药剂筛选研究[J]. 新疆农业科学,2016,53(5):866−876. [HAN S, YUSHANJIANG M M T, PAN Y, et al. Identification of postharvest pathogens and screening of fungicides for fresh apricot in Xinjiang[J]. Xinjiang Agricultural Sciences,2016,53(5):866−876. HAN S, YUSHANJIANG M M T, PAN Y, et al. Identification of postharvest pathogens and screening of fungicides for fresh apricot in Xinjiang[J]. Xinjiang Agricultural Sciences, 2016, 53(5): 866-876.
[8]	程元, 淮稳霞, 姚艳霞, 等. 新疆巩留县杏果实斑点病病原菌鉴定[J]. 林业科学研究,2019,32(2):117−122. [CHENG Y, HUAI W X, YAO Y X, et al. The pathogen identification of apricot fruit spots disease in Gongliu County, Xinjiang[J]. Forest Research,2019,32(2):117−122. CHENG Y, HUAI W X, YAO Y X, et al. The pathogen identification of apricot fruit spots disease in Gongliu County, Xinjiang[J]. Forest Research, 2019, 32(2): 117-122.
[9]	吴思雅, 阿衣木古丽·艾赛提, 郑灿龙, 等. 新疆赛买提杏采后主要病原真菌的分离及鉴定[J]. 食品工业科技,2016,37(1):149−152. [WU S Y, AYIMUGULI A S T, ZHENG S L, et al. Identification of main pathogenic fungus from post-harvest “Saimaiti” apricot fruits[J]. Science and Technology of Food Industry,2016,37(1):149−152. WU S Y, AYIMUGULI A S T, ZHENG S L, et al. Identification of main pathogenic fungus from post-harvest “Saimaiti” apricot fruits[J]. Science and Technology of Food Industry, 2016, 37(1): 149-152.
[10]	WU F, LI S C, MA Q L, et al. First report of Fusarium oxysporum causing fruit rot on apricot (Prunus armeniaca) in China[J]. Plant Disease,2022,106(8):2261−2261.
[11]	贾盼盼, 刘晓丹, 吝晨晨, 等. 壳寡糖对杏果实采后主要病原菌抑菌作用的研究[J]. 新疆农业科学,2012,49(2):290−295. [JIA P P, LIU X D, LIN C C, et al. Study on influencing factors of the antifungal activity on the main postharvest disease-producing fungus of apricot fruit by oligochitosan[J]. Xinjiang Agricultural Sciences,2012,49(2):290−295. JIA P P, LIU X D, LIN C C, et al. Study on influencing factors of the antifungal activity on the main postharvest disease-producing fungus of apricot fruit by oligochitosan[J]. Xinjiang Agricultural Sciences, 2012, 49(2): 290-295.
[12]	CHAN Y, GUO Q, WEI J, et al. Inhibitory effect and mechanism of nitric oxide (NO) fumigation on fungal disease in Xinjiang Saimaiti dried apricots[J]. LWT-Food Science and Technology,2019,116:108507. doi: 10.1016/j.lwt.2019.108507
[13]	LI Y C, MA Y Y, ZHANG T T, et al. Exogenous polyamines enhance resistance to Alternaria alternata by modulating redox homeostasis in apricot fruit[J]. Food Chemistry,2019,301:125303. doi: 10.1016/j.foodchem.2019.125303
[14]	SCHOCH C L, SEIFERT K A, HUHNDORF S. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi[J]. Proceedings of the National Academy of Sciences of the United States of America,2012,109(16):6241−6246. doi: 10.1073/pnas.1117018109
[15]	DURAIRAJ K, VELMURUGAN P, VEDHANAYAKISRI KA, et al. Molecular and phenotypic characterization of pathogenic fungal strains isolated from ginseng root rot[J]. Physiological and Molecular Plant Pathology,2019,105:28−33. doi: 10.1016/j.pmpp.2018.11.005
[16]	SOLAIRAJ D, LEGRAND N N G, YANG Q Y, et al. Isolation of pathogenic fungi causing postharvest decay in table grapes and in vivo biocontrol activity of selected yeasts against them[J]. Physiological and Molecular Plant Pathology,2020,110:101478. doi: 10.1016/j.pmpp.2020.101478
[17]	黄津津, 姚婷, 王友升. 10株葡萄源丝状真菌分离鉴定及其致病力分析[J]. 食品科学技术学报,2018,36(4):46−54. [HUANG J J, YAO T, WANG Y S. Internal transcribed spacer region sequencing and phylogenetic analysis of 10 strains of Beauveria spp[J]. Journal of Food Science and Technology,2018,36(4):46−54. doi: 10.3969/j.issn.2095-6002.2018.04.007 HUANG J J, YAO T, WANG Y S. Internal transcribed spacer region sequencing and phylogenetic analysis of 10 strains of Beauveria spp[J]. Journal of Food Science and Technology, 2018, 36(4): 46-54. doi: 10.3969/j.issn.2095-6002.2018.04.007
[18]	王友升, 张燕, 陈玉娟. 5株桃、李果实采后褐腐病菌鉴定、rDNA ITS序列与碳源代谢指纹图谱分析[J]. 食品科学,2012,33(16):246−250. [WANG Y S, ZHANG Y, CHEN Y J, et al. Identification, rDNA ITS analysis and carbon metabolic fingerprinting of five Monilinia fructicola strains isolated from postharvest peach and plum fruits[J]. Food Science,2012,33(16):246−250. WANG Y S, ZHANG Y, CHEN Y J, et al. Identification, rDNA ITS analysis and carbon metabolic fingerprinting of five Monilinia fructicola strains isolated from postharvest peach and plum fruits [J]. Food Science, 2012(16): 246-250.
[19]	王友升, 郭晓敏, 姚子鹏, 等. 1株桃果实采后病原真菌的鉴定以及碳源代谢指纹图谱分析[J]. 北京工商大学学报(自然科学版),2011,29(1):47−53. [WANG Y S, GUO X M, YAO Z P, et al. Identification and carbon metabolic fingerprinting analysis of a pathogen isolated from postharvest peach fruit[J]. Journal of Food Science and Technology,2011,29(1):47−53. WANG Y S, GUO X M, YAO Z P, et al. Identification and carbon metabolic fingerprinting analysis of a pathogen isolated from postharvest peach fruit [J]. Journal of Food Science and Technology, 2011, 29(1): 47-53.
[20]	魏景超. 真菌鉴定手册 [M]. 上海: 上海科技出版社, 1979: 60−501. WEI J C. Fungal identification manual[M]. Shanghai Scientific & Technical Publishers, 1979: 60−501.
[21]	王宇, 郭良栋. 内生真菌EPICOCCUM NIGRUM的形态与分子鉴定[J]. 菌物学报,2004,23(4):474−479. [WANG Y, GUO L D. Morphological and molecular identification of an endophytic fungus EPICOCCUM NIGRUM[J]. Mycosystema,2004,23(4):474−479. doi: 10.3969/j.issn.1672-6472.2004.04.005 WANG Y, GUO L D. Morphological and molecular identification of an endophytic fungus EPICOCCUM NIGRUM[J]. Mycosystema, 2004, 23(4): 474-479. doi: 10.3969/j.issn.1672-6472.2004.04.005
[22]	CHAISIRI C, LIU X Y, LIN Y, et al. Phylogenetic and haplotype network analyses of Diaporthe eres species in China based on sequences of multiple loci[J]. Biology-Basel,2021,10(3):179.
[23]	GOS H, BRYK H, MICHALECKA M, et al. First report of Diaporthe eres a new pathogen causing rot of apples during storage period in Poland[J]. Journal of Plant Pathology,2021,103(6):393−394.
[24]	XIAO Y S, HUO G H, LIU L L, et al. First report of postharvest fruit rot disease of yellow peach caused by Diaporthe eres in China[J]. Plant Disease,2021,106(7):1983−1983.
[25]	BERTETTI D, GUARNACCIA V, SPADARO D, et al. First report of fruit rot in European pear caused by Diaporthe eres in Italy[J]. Plant Disease,2018,102(12):2651−2651.
[26]	LORENZINI M, ZAPPAROLI G. Identification of Pestalotiopsis bicilita, Diplodia seriata and Diaporthe eres causing fruit rot in withered grapes in Italy[J]. European Journal of Plant Pathology,2018,151(4):1089−1093. doi: 10.1007/s10658-017-1416-1
[27]	YAMIN D U, WANG X, GUO Y, et al. Biological and molecular characterization of seven Diaporthe species associated with kiwifruit shoot blight and leaf spot in China[J]. Phytopathologia Mediterranea,2021,60(2):177−198. doi: 10.36253/phyto-12013
[28]	严雪瑞, 王旭, 胡梦琼, 等. 蓝莓间座壳芽枯病病原菌鉴定及其生物学特性[J]. 植物病理学报,2015,45(5):556−560. [YAN X R, WANG X, HU M Q, et al. Identification and biological characteristic of blueberry Diaporthe bud blight pathogen[J]. Acta Phytopathologica Sinica,2015,45(5):556−560. YAN X R, WANG X, HU M Q, et al. Identification and biological characteristic of blueberry Diaporthe bud blight pathogen[J]. Acta Phytopathologica Sinica, 2015, 45(5): 556-560.
[29]	翟立峰. 梨轮纹病与干腐病的病原关系及轮纹病菌携带真菌病毒多样性研究[D]. 武汉: 华中农业大学, 2016. ZHAI L F. Relations among four Botryosphaeria species isolated from pear stem wart and stem canker and diversity of mycoviruses from Botryosphaeria dothidea[D]. Wuhan: Huazhong Agricultural University, 2016.
[30]	刘娜, 谢国芳, 袁孟孟, 等. 猕猴桃软腐病发病过程内生真菌多样性分析[J]. 食品科技,2020,45(4):31−36. [LIU N, XIE G F, YUAN M M, et al. Diversity of endophytic fungal in the pathogenesis of soft rot in kiwifruit[J]. Food Science and Technology,2020,45(4):31−36. doi: 10.13684/j.cnki.spkj.2020.04.006 LIU N, XIE G F, YUAN M M, et al. Diversity of endophytic fungal in the pathogenesis of soft rot in kiwifruit[J]. Food Science and Technology, 2020, 45(4): 31-36. doi: 10.13684/j.cnki.spkj.2020.04.006
[31]	孙秋玲, 杨芝霓, 唐利华, 等. 芒果蒂腐病病原葡萄座腔菌科真菌种类鉴定[J]. 植物病理学报,2022,52(6):1009−1012. [SUN Q, YANG Z N, TANG L H, et al. Identification of pathogenic Botryosphaeriaceae species from mango stem-end rot[J]. Acta Phytopathologica Sinica,2022,52(6):1009−1012. SUN Q, YANG Z N, TANG L H, et al. Identification of pathogenic Botryosphaeriaceae species from mango stem-end rot[J]. Acta Phytopathologica Sinica, 2022, 52(6): 1009-1012.
[32]	姚子鹏, 王友升, 郭晓敏, 等. 桃果实贮藏期间病原真菌的ITS rDNA序列分析与鉴定[J]. 中国食品学报,2011,11(4):172−178. [YAO Z P, WANG Y S, GUO X M, et al. ITS rDNA sequence analysis and identification of pathogens for postharvest diseases of peach fruit[J]. Journal of Chinese Institute of Food Science and Technology,2011,11(4):172−178. doi: 10.3969/j.issn.1009-7848.2011.04.026 YAO Z P, WANG Y S, GUO X M, et al. ITS rDNA sequence analysis and identification of pathogens for postharvest diseases of peach fruit[J]. Journal of Chinese Institute of Food Science and Technology, 2011, 11(4): 172-178. doi: 10.3969/j.issn.1009-7848.2011.04.026
[33]	姚婷, 丁凤兰, 王珂, 等. 5株枣果实采后病原真菌的分离鉴定及ITS区序列分析[J]. 食品科学技术学报,2022,40(5):62−70. [YAO T, DING F L, WANG K, et al. Morphological characterization and rDNA ITS sequence analysis of five pathogenic fungi isolated from infected jujube fruit[J]. Journal of Food Science and Technology,2022,40(5):62−70. doi: 10.12301/spxb202100734 YAO T, DING F L, WANG K, et al. Morphological characterization and rDNA ITS sequence analysis of five pathogenic fungi isolated from infected jujube fruit[J]. Journal of Food Science and Technology, 2022, 40(5): 62-70. doi: 10.12301/spxb202100734
[34]	LORENZINI M, ZAPPAROLI G. Occurrence and infection of Cladosporium, Fusarium, Epicoccum and Aureobasidium in withered rotten grapes during post-harvest dehydration[J]. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology,2015,108(5):1171−1180. doi: 10.1007/s10482-015-0570-8
[35]	任苗苗, 闫思远, 李嘉泓, 等. 宁夏‘灵武长枣’采后病原真菌的分离与鉴定[J]. 中国果树,2020(5):93−97, 102, 142. [REN M M, YAN S Y, LI J H, et al. Isolation and identification of postharvest pathogenic fungi from ‘Lingwuchan’ jujube in Ningxia[J]. China Fruits,2020(5):93−97, 102, 142. doi: 10.16626/j.cnki.issn1000-8047.2020.05.017 REN M M, YAN S Y, LI J H, et al. Isolation and identification of postharvest pathogenic fungi from ‘Lingwuchan’ jujube in Ningxia[J]. China Fruits, 2020(5): 93-97, 102, 142. doi: 10.16626/j.cnki.issn1000-8047.2020.05.017
[36]	GAO X, CUI Q, CAO Q Z, et al. Transcriptome-wide analysis of Botrytis elliptica responsive microRNAs and their targets in Lilium Regale Wilson by high-throughput sequencing and degradome analysis[J]. Frontiers in Plant Science,2017,8:753. doi: 10.3389/fpls.2017.00753
[37]	姚婷, 陈其葳, 张燕, 等. 3株果实采后葡萄孢属真菌ITS区rDNA序列与碳源代谢指纹图谱分析[J]. 食品科学技术学报,2017,35(4):49−55. [YAO T, CHEN Q W, ZHANG Y, et al. Analysis of rDNA ITS and carbon metabolic fingerprinting of Botrytis Nees isolated from three fruits[J]. Journal of Food Science and Technology,2017,35(4):49−55. doi: 10.3969/j.issn.2095-6002.2017.04.007 YAO T, CHEN Q W, ZHANG Y, et al. Analysis of rDNA ITS and carbon metabolic fingerprinting of Botrytis Nees isolated from three fruits [J]. Journal of Food Science and Technology, 2017, 35(4): 49-55. doi: 10.3969/j.issn.2095-6002.2017.04.007