Simultaneous Determination of 17 <i>β</i>-Receptor Agonists in Meat by QuEChERS EMR-Lipid with Isotope Dilution-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

MAO Rui; LIN Hao; LIU Chuan; YAO Jing; XIAO Quanwei; DAI Qin

doi:10.13386/j.issn1002-0306.2022090195

Volume 44 Issue 15

Jul. 2023

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Science and Technology of Food Industry > 2023 > 44(15): 320-328. > DOI: 10.13386/j.issn1002-0306.2022090195

MAO Rui, LIN Hao, LIU Chuan, et al. Simultaneous Determination of 17 β-Receptor Agonists in Meat by QuEChERS EMR-Lipid with Isotope Dilution-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2023, 44(15): 320−328. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090195.

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Simultaneous Determination of 17 β-Receptor Agonists in Meat by QuEChERS EMR-Lipid with Isotope Dilution-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

Key Laboratory of Chemical Metrology and Applications on Nutrition and Health for State Market Regulation, Chengdu Institute of Food Inspection, Chengdu 611130, China

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Received Date: September 18, 2022
Available Online: June 05, 2023

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Abstract

Objective: A method for simultaneous determination of 17 β-receptor agonists in meat was established by QuEChERS EMR-Lipid with isotope dilution-ultra performance liquid chromatography-tandem mass spectrometry. Methods: The samples were added to ammonium acetate buffer solution (pH5.2) and enzymatic hydrolyzed by β-glucuronidase and arysulfatase. The hydrolysates were extracted by acetonitrile containing 5% formic acid, and purified by QuEChERS EMR-Lipid. The analyses were separated by CORTECS™ UPLC^® C₁₈ chromatographic column with gradient elution using 0.1% formic acid and methanol as the mobile phase. The target compounds were monitored in scheduled MRM mode. Then the internal standard method was used for quantitative analysis. Results: The linear regression correlation coefficients for the 17 β-receptor agonists were all higher than 0.99 in the range from 0 ng/mL to 20 ng/mL. The limits of detection were 0.01~0.09 μg/kg and the limits of quantification were 0.05~0.31 μg/kg. The average recoveries at three spiked levels of 0.5, 2.0 and 5.0 μg/kg ranged from 81.7% to 111.8% with the relative standard deviations of 0.8%~10.3% (n=6). No β-receptor agonists were detected in 100 batches of fresh meat. Conclusion: The method has the advantages of simple pretreatment steps and good purification effect. It shortened enzymolysis time, improved sample detection efficiency. The internal standard method was accurate and reliable. It was suitable for simultaneous determination of β-receptor agonists in a large number of meat samples.

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