XIE Dan, WU Cheng, BI Yuanlin, et al. Study on the Correlation between Microbial Community Succession and Physicochemical Factors during the Storage of Maotai-flavor Liquor in Daqu at High Temperature[J]. Science and Technology of Food Industry, 2023, 44(15): 151−158. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090153.
Citation: XIE Dan, WU Cheng, BI Yuanlin, et al. Study on the Correlation between Microbial Community Succession and Physicochemical Factors during the Storage of Maotai-flavor Liquor in Daqu at High Temperature[J]. Science and Technology of Food Industry, 2023, 44(15): 151−158. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090153.

Study on the Correlation between Microbial Community Succession and Physicochemical Factors during the Storage of Maotai-flavor Liquor in Daqu at High Temperature

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  • Received Date: September 14, 2022
  • Available Online: May 29, 2023
  • Objective: Through analyzing the physicochemical properties and microbial changes of high temperature Daqu of Maotai-flavored during the storage (0~150 days), the paper aimed at comprehending the aging and maturity law of Daqu. Methods: Based on the traditional cultivable method and high-throughput sequencing technology, the research analyzed the succession law of microbial community structure during the storage of Daqu, and the relationships between physicochemical factors and microorganisms by combining the correlation. Results: The number of cultivable microorganisms showed a trend of first increased and then declined with the increased storage time during the storage. The temperature and saccharification index showed a trend of first increased and then declined, moisture and acidity showed a declined trend with increasing storage time. Meanwhile, the main leading bacteria genus during the storage were Kroppenstedtia, Lactobacillus, Staphylococcus and Enterobacter respectively, the main leading fungus genus were Thermomyces, Trichomonascus and Byssochlamys respectively. There was a complicated relationship between microbial community and physicochemical indicators, of which Bacillus, Kroppenstedtia, Aspergillus and Trichomonascus were positively correlated with temperature, Byssochlamys and Dipodascus were positively correlated with temperature, moisture, acidity and liquefaction power. Thermocycles was negatively correlated with temperature, moisture, and acidity. Conclusion: The research revealed the microbial community composition status and the relationships between physicochemical factors of high-temperature Daqu of Maotai-flavored during the storage, which provided a theoretical reference for further introducing the post ripeness mechanism of high-temperature Daqu of Maotai-flavored.
  • [1]
    李静心, 王艳丽, 何宏魁, 等. 基于高通量测序技术解析高温大曲和中高温大曲的真菌群落结构[J]. 食品与发酵工业,2018,44(12):52−59. [LI J X, WANG Y L, HE H K, et al. Analysis of fungal community structure of high temperature Daqu and medium high temperature Daqu based on high-throughput sequencing technology[J]. Food and Fermentation Industry,2018,44(12):52−59. doi: 10.13995/j.cnki.11-1802/ts.018043

    LI J X, WANG Y L, HE H K, et al. Analysis of fungal community structure of high temperature Daqu and medium high temperature Daqu based on high-throughput sequencing technology [J]. Food and Fermentation Industry, 2018, 44 (12): 52-59. doi: 10.13995/j.cnki.11-1802/ts.018043
    [2]
    WU Q, CHEN L, XU Y. Yeast community associated with the solid state fermentation of traditional Chinese Maotai-flavor liquor[J]. International Journal of Food Microbiology,2013,166(2):323−330. doi: 10.1016/j.ijfoodmicro.2013.07.003
    [3]
    ZHANG X, ZHAO J, DU X. Barcoded pyrosequencing analysis of the bacterial community of Daqu for light-flavour Chinese liquor[J]. Letters in Applied Microbiology,2014,58(6):549−55. doi: 10.1111/lam.12225
    [4]
    LIU P, ZHANG L, DU X, et al. Dynamic analysis of physicochemical and biochemical indices and microbial communities of light-flavor Daqu during storage[J]. Journal of the American Society of Brewing Chemists,2019,77(4):1−8.
    [5]
    刘鹏烩. 清香大曲贮存期的变化和大曲糖化酶功能菌及快速检测的相关研究[D]. 太原: 山西师范大学, 2018.

    LIU P H. Dynamic changes of light-flavor Daqu during storage period and researches on the glucohydrolace functional bacteria and rapid detection[D]. Taiyuan: Shanxi Normal University, 2018.
    [6]
    王晓丹, 雷安亮, 班世栋等. 酱香型大曲细菌的多样性[J]. 食品与发酵工业,2017,43(5):70−75. [WANG X D, LEI A L, BAN S D, et al. Bacterial diversity of Maotai flavor Daqu[J]. Food and Fermentation Industry,2017,43(5):70−75. doi: 10.13995/j.cnki.11-1802/ts.201705012

    WANG X D, LEI A L, BAN S D, et al. Bacterial diversity of Maotai flavor Daqu[J]. Food and Fermentation Industry, 2017, 43 (5): 70-75. doi: 10.13995/j.cnki.11-1802/ts.201705012
    [7]
    张丽, 卢红梅, 陈莉, 等. 酱香型大曲贮存过程中发酵性能变化的研究[J]. 现代食品科技,2017,33(9):231−237. [ZHANG L, LU H M, CHEN L, et al. Study on changes in the biochemical properties of Maotai-flavor Daqu during storage[J]. Modern Food Science and Technology,2017,33(9):231−237. doi: 10.13982/j.mfst.1673-9078.2017.9.034

    ZHANG L, LU H M, CHEN L, et al. Study on changes in the biochemical properties of Maotai-flavor Daqu during storage[J]. Modern Food Science and Technology, 2017, 33(9): 231-237. doi: 10.13982/j.mfst.1673-9078.2017.9.034
    [8]
    刘建文, 吴生文, 朱力红, 等. 特香型大曲中纤维素酶、半纤维素酶、蛋白酶、酯化酶活力变化规律的研究[J]. 酿酒,2013,40(5):40−42. [LIU J W, WU S W, ZHU L H, et al. Study on the change law of cellulase, hemicellulase, protease and esterase activities in special flavor Daqu[J]. Liquor Making,2013,40(5):40−42. doi: 10.3969/j.issn.1002-8110.2013.05.012

    LIU J W, WU S W, ZHU L H, et al. Study on the change law of cellulase, hemicellulase, protease and esterase activities in special flavor Daqu[J]. Liquor Making, 2013, 40(5): 40-42. doi: 10.3969/j.issn.1002-8110.2013.05.012
    [9]
    梁丽文, 缪礼鸿, 杨团元, 等. 浓酱兼香型白酒不同储存期的高温大曲微生物群落结构与发酵特征分析[J]. 酿酒科技,2016(7):37−41. [LIANG L W, MIAO L H, YANG T Y, et al. Analysis of microbial community structure and fermentation characteristics of high temperature Daqu in different storage periods of thick sauce and flavor Baijiu[J]. Liquor-Making Science & Technology,2016(7):37−41. doi: 10.13746/j.njkj.2016084

    LIANG L W, MIAO L H, YANG T Y, et al. Analysis of microbial community structure and fermentation characteristics of high temperature Daqu in different storage periods of thick sauce and flavor Baijiu [J]. Liquor-Making Science & Technology, 2016(7): 37-41 doi: 10.13746/j.njkj.2016084
    [10]
    梁晨, 杜海, 徐岩. 大曲贮存过程中原核微生物群落结构及风味成分演替规律[J]. 微生物学通报,2017,44(2):384−393. [LIANG C, DU H, XU Y. The succession of procaryotic microbial community and the flavor components in the storage process of Daqu[J]. Journal of Microbiology,2017,44(2):384−393. doi: 10.13344/j.microbiol.china.160163

    LIANG C, DU H, XU Y. The succession of procaryotic microbial community and the flavor components in the storage process of Daqu[J]. Journal of Microbiology, 2017, 44(2): 384-393. doi: 10.13344/j.microbiol.china.160163
    [11]
    陈晓茹, 黄钧, 周荣清, 等. 强化曲贮存过程中群落与代谢组分的变化规律[J]. 食品与发酵工业,2022,48(3):63−69. [CHENG X R, HUANG J, ZHOU Q R, et al. Changes of community and metabolic components during the storage of fortified Daqu[J]. Food and Fermentation Industry,2022,48(3):63−69. doi: 10.13995/j.cnki.11-1802/ts.028640

    CHENG X R, HUANG J, ZHOU Q R, et al. Changes of community and metabolic components during the storage of fortified Daqu[J]. Food and Fermentation Industry, 2022, 48(3): 63-69. doi: 10.13995/j.cnki.11-1802/ts.028640
    [12]
    QB/T 4257-2011, 酿酒大曲通用分析方法[S]. 中华人民共和国轻工行业标准, 2011.

    QB/T 4257-2011, General analysis method of liquor making Daqu[S]. Light Industry Standards of the People's Republic of China, 2011.
    [13]
    赵金松, 张良, 孙啸涛, 等. 酱香型大曲微生物群落特征与理化指标的相关性研究[J]. 基因组学与应用生物学,2019,38(1):199−204. [ZHAO J S, ZHANG L, SUN X T, et al. Study on the correlation between microbial community characteristics and physical and chemical indicators of Maotai flavor Daqu[J]. Genomics and Applied Biology,2019,38(1):199−204. doi: 10.13417/j.gab.038.000199

    ZHAO J S, ZHANG L, SUN X T, et al. Study on the correlation between microbial community characteristics and physical and chemical indicators of Maotai flavor Daqu [J]. Genomics and Applied Biology, 2019, 38(1): 199-204. doi: 10.13417/j.gab.038.000199
    [14]
    吴成, 程平言, 谢丹, 等. 酱香型白酒四轮次堆积发酵理化因子、风味物质与微生物群落相关性研究[J/OL]. 食品科学: 1−12 [2022-9-13]. http://kns.cnki.net/kcms/detail/11.2206.TS.20220622.0835.002.html

    WU C, CHENG P Y, XIE D, et al. Relationship analysis among physicochemical parameters, flavor compounds and microbial community during fourth round heap fermentation of Jiang-flavored Baijiu[J/OL]. Food Science: 1−12 [2022-9-13]. http://kns.cnki.net/kcms/detail/11.2206.TS.20220622.0835.002.html
    [15]
    WANG C, WU C, QIU S. Yeast diversity investigation of Vitis davidii Föex during spontaneous fermentations using culture-dependent and high-throughput sequencing approaches[J]. Food Research International,2019,126:108582. doi: 10.1016/j.foodres.2019.108582
    [16]
    王颖, 邱勇, 王隆, 等. 不同产区酱香型高温大曲黑、白、黄曲的理化、挥发性成分差异性分析[J]. 中国调味品,2022,47(6):155−159. [WANG Y, QIU Y, WANG L, et al. Analysis on the difference of physicochemical indexes and volatile components of black, white and yellow koji at hight temperature with Maotai flavor in different production areas[J]. Chinese Condiments,2022,47(6):155−159. doi: 10.3969/j.issn.1000-9973.2022.06.029

    WANG Y, QIU Y, WANG L, et al. Analysis on the difference of physicochemical indexes and volatile components of black, white and yellow koji at hight temperature with Maotai Flavor in different production areas [J]. Chinese Condiments, 2022, 47 (6): 155-159. doi: 10.3969/j.issn.1000-9973.2022.06.029
    [17]
    ZHANG W X, WU Z Y, ZHANG Q S, et al. Combination of newly developed high quality Fuqu with traditional Daqu for Luzhou-flavor liquor brewing[J]. World Journal of Microbiology & Biotechnology,2009,25(10):1721−1726.
    [18]
    章肇敏, 吴生文, 李科发. 特香型白酒酿造过程中物质变化规律研究[J]. 食品工业科技,2012,33(9):108−110, 113. [ZHANG Z M, WU S W, LI K F. Study on the change rules of chemical composition of site-flavor fermented grains in fermentation process[J]. Food Industry Science and Technology,2012,33(9):108−110, 113. doi: 10.13386/j.issn1002-0306.2012.09.082

    ZHANG Z M, WU S W, LI K F. Study on the change rules of chemical composition of site-flavor fermented grains in fermentation process[J]. Food Industry Science and Technology, 2012, 33(9): 108-110, 113. doi: 10.13386/j.issn1002-0306.2012.09.082
    [19]
    范文来, 徐岩. 大曲酶系研究的回顾与展望[J]. 酿酒,2000(3):35−40. [FAN W L, XU Y. Review and prospect of Daqu enzyme system research[J]. Liquor Making,2000(3):35−40.

    FAN W L, XU Y. Review and prospect of Daqu enzyme system research[J]. Liquor Making, 2000(3): 35-40.
    [20]
    李洁, 武轩. 传统凤型大曲后熟期成熟度评价及最佳贮藏期研究[J]. 食品与发酵工业,2020,46(24):97−102. [LI J, WU X. Maturity evaluation and optimum storage time of traditional Feng-type Daqu in post-maturity period[J]. Food and Fermentation Industries,2020,46(24):97−102. doi: 10.13995/j.cnki.11-1802/ts.024568

    LI J, WU X. Maturity evaluation and optimum storage time of traditional Feng-type Daqu in post- maturity period[J]. Food and Fermentation Industries, 2020, 46(24): 97-102. doi: 10.13995/j.cnki.11-1802/ts.024568
    [21]
    左乾程, 黄永光, 郭敏, 等. 酱香型白酒机械化制曲发酵细菌群落的演替[J]. 食品科学,2021,42(18):150−156. [ZUO Q C, HUANG Y G, GUO M, et al. The succession of bacterial community in Maotai flavor Baijiu mechanized koji fermentation[J]. Food Science,2021,42(18):150−156. doi: 10.7506/spkx1002-6630-20200705-059

    ZUO Q C, HUANG Y G, GUO M, et al. The succession of bacterial community in Maotai flavor Baijiu mechanized koji fermentation [J]. Food Science, 2021, 42 (18): 150-156. doi: 10.7506/spkx1002-6630-20200705-059
    [22]
    王晓丹, 班世栋, 周鸿翔, 等. 贵州省遵义地区3个酱香型大曲细菌群落的比较分析[J]. 食品科学,2016,37(7):110−116. [WANG X D, BAN S D, ZHOU H X, et al. Comparative analysis of bacterial communities of three Maotai flavor Daqu in Zunyi area, Guizhou Province[J]. Food Science,2016,37(7):110−116.

    WANG X D, BAN S D, ZHOU H X, et al. Comparative analysis of bacterial communities of three Maotai flavor Daqu in Zunyi area, Guizhou Province[J]. Food Science, 2016, 37(7): 110-116.
    [23]
    张立强, 冉茂芳, 王松涛, 等. 泸州老窖大曲中高产纤溶酶菌株的分离及鉴定[J]. 酿酒科技,2021(3):17−24. [ZHANG L Q, RAN M F, WANG S T, et al. Isolation and identification of high fibrinolytic enzyme producing strains from Luzhou Laojiao Daqu[J]. Liquor-Making Science & Technology,2021(3):17−24. doi: 10.13746/j.njkj.2021033

    ZHANG L Q, RAN M F, WANG S T, et al. Isolation and identification of high fibrinolytic enzyme producing strains from Luzhou Laojiao Daqu[J]. Liquor-Making Science & Technology, 2021(3): 17-24. doi: 10.13746/j.njkj.2021033
    [24]
    李子健, 黄丹, 张曼, 等. 中高温大曲中两株耐热霉菌的分离鉴定及产酶特性比较[J]. 食品与发酵科技,2020,56(3):1−7. [LI Z J, HUANG D, ZHANG M, et al. Isolation and Identification of two thermostable fungi from medium and high temperature Daqu and comparison of their enzymatic characteristics[J]. Food and Fermentation Technology,2020,56(3):1−7.

    LI Z J, HUANG D, ZHANG M, et al. Isolation and Identification of two thermostable fungi from medium and high temperature Daqu and comparison of their enzymatic characteristics[J]. Food and Fermentation Technology, 2020, 56(3): 1-7.
    [25]
    施思, 彭智辅, 乔宗伟, 等. 浓香型大曲贮藏过程中糖化力发酵力变化及真菌多样性分析[J]. 食品与发酵工业,2017,43(5):76−79. [SHI S, PENG Z F, QIAO Z W, et al. Changes of saccharifying and fermenting power of Luzhou flavor Daqu during storage and analysis of fungal diversity[J]. Food and Fermentation Industries,2017,43(5):76−79. doi: 10.13995/j.cnki.11-1802/ts.201705013

    SHI S, PENG Z F, QIAO Z W, et al. Changes of saccharifying and fermenting power of Luzhou flavor Daqu during storage and analysis of fungal diversity[J]. Food and Fermentation Industries, 2017, 43(5): 76-79. doi: 10.13995/j.cnki.11-1802/ts.201705013
    [26]
    董大伟, 王晓慧, 苏葛, 等. 中高温大曲存储后的感官质量研究[J]. 酿酒科技,2018(12):39−44. [DONG D W, WANG X H, SU G, et al. Research on sensory quality of medium high-temperature Daqu after storage[J]. Liquor-Making Science & Technology,2018(12):39−44. doi: 10.13746/j.njkj.2018131

    DONG D W, WANG X H, SU G, et al. Research on sensory quality of medium high-temperature Daqu after storage [J]. Liquor-Making Science & Technology, 2018(12): 39-44. doi: 10.13746/j.njkj.2018131
    [27]
    NURSTEN H E. The Maillard reaction[electronic resource]: Chemistry, biochemistry, and implications/. 2005.
    [28]
    MARCHAND S, REVELl G D, VERCAUTEREN J, et al. Possible mechanism for involvement of cysteine in aroma production in wine[J]. Journal of Agricultural & Food Chemistry,2002,50(21):6160−6164.
    [29]
    CHENG L, XU Y, WU Q, et al. Improving flavor metabolism of Saccharomyces cerevisiae by mixed culture with Bacillus licheniformis for Chinese Maotai-flavor liquor making[J]. J Ind Microbiol Biotechnol,2015,42(12):1601−1608. doi: 10.1007/s10295-015-1647-0
    [30]
    郭敏. 基于高通量测序对酱香大曲制曲微生态多样性的研究[D]. 贵阳: 贵州大学, 2018.

    GUO M. Study on microecological diversity of Maotai flavor Daqu based on high-throughput sequencing[D]. Guiyang: Guizhou University, 2018.
    [31]
    童炳丽, 刘济明, 陈敬忠, 等. 米槁根际土壤真菌多样性及其与果实药用活性成分含量的相关性分析[J]. 菌物学报,2019,38(7):1058−1070. [TONG B L, LIU J M, CHEN J Z, et al. Fungal diversity in rhizosphere soil of Migao and its correlation with the content of medicinal active components in fruit[J]. Acta mycologica Sinica,2019,38(7):1058−1070. doi: 10.13346/j.mycosystema.190062

    TONG B L, LIU J M, CHEN J Z, et al. Fungal diversity in rhizosphere soil of Migao and its correlation with the content of medicinal active components in fruit [J]. Acta mycologica Sinica, 2019, 38(7): 1058-1070. doi: 10.13346/j.mycosystema.190062
    [32]
    朱治宇, 黄永光. 茅台镇酱香白酒不同轮次主酿区可培养霉菌种群结构多样性[J]. 食品科学,2020,41(22):184−192. [ZHU Z Y, HUANG Y G. Structure and diversity of culturable mold communities in different fermentation rounds in main Maotai-flavored liquor-brewing areas of Maotai Town[J]. Food Science,2020,41(22):184−192. doi: 10.7506/spkx1002-6630-20190918-226

    ZHU Z Y, HUANG Y G. Structure and diversity of culturable mold communities in different fermentation rounds in main Maotai-flavored liquor-brewing areas of Maotai town[J]. Food Science, 2020, 41(22): 184-192. doi: 10.7506/spkx1002-6630-20190918-226
    [33]
    唐小飞. 西藏热泉嗜热真菌分离鉴定、产酶及生物活性初步研究[D]. 汉中: 陕西理工大学, 2019.

    TANG X F. Isolation, identification, enzyme production and biological activity of thermophilic fungi from hot springs in Tibet[D]. Hanzhong: Shaanxi University of Technology, 2019.
    [34]
    张芸曌. 中高温大曲主发酵期微生物群落与环境因子及理化性质的关联性研究[D]. 自贡: 四川轻化工大学, 2019.

    ZHANG Y Z. Study on the correlation between microbial community and environmental factors and physicochemical properties during the main fermentation period of medium-high temperature Daqu[D]. Zigong: Sichuan University of Science &Engineering, 2019.
    [35]
    杜向军, 明红梅, 马浩, 等. 机制与人工大曲曲块部位间微生物群落结构差异及关键影响因素分析[J/OL]. 食品与发酵工业: 1−10 [2022-7-22]. DOI: 10.13995/j.cnki.11-1802/ts.031775.

    DU X J, MING H M, MA H, et al. Analysis of microbial community structure differences and key influencing factors among different parts of mechanical Daqu and artificial Daqu[J/OL]. Food and Fermentation Industries: 1−10 [2022-7-22]. DOI: 10.13995/j.cnki.11-1802/ts.031775.
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