Analysis of Anthocyanin Accumulation and Gene Expression of Anthocyanin Synthesis Pathway during Fruit Ripening of 'Benihoppe' Strawberry

PENG Zhenzhen; ZHONG Chuanfei; WANG Baogang; LI Wensheng; ZHOU Jiahua; FU Daqi; CHANG Hong; WANG Yunxiang

doi:10.13386/j.issn1002-0306.2022090106

Volume 44 Issue 14

Jul. 2023

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Science and Technology of Food Industry > 2023 > 44(14): 346-354. > DOI: 10.13386/j.issn1002-0306.2022090106

PENG Zhenzhen, ZHONG Chuanfei, WANG Baogang, et al. Analysis of Anthocyanin Accumulation and Gene Expression of Anthocyanin Synthesis Pathway during Fruit Ripening of 'Benihoppe' Strawberry[J]. Science and Technology of Food Industry, 2023, 44(14): 346−354. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090106.

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Analysis of Anthocyanin Accumulation and Gene Expression of Anthocyanin Synthesis Pathway during Fruit Ripening of 'Benihoppe' Strawberry

PENG Zhenzhen^{1, 2,},
ZHONG Chuanfei³,
WANG Baogang^{1, 4, 5},
LI Wensheng^{1, 4, 5},
ZHOU Jiahua^{1, 4, 5},
FU Daqi²,
CHANG Hong^{1, 4, 5},
WANG Yunxiang^{1, 4, 5, ,}

1.
Institute of Agricultural Products Processing and Food Nutrition, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China
2.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
3.
Institute of Forestry and Fruit Trees, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100093, China
4.
Beijing Key Laboratory of Fruits and Vegetable Storage and Processing, Beijing 100097, China
5.
Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture and Rural Affairs, Beijing 100097, China

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Received Date: September 12, 2022
Available Online: May 18, 2023

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Abstract

Abstract

To explore the genes related to anthocyanin accumulation and synthesis during the ripening process of strawberry fruit, qualitative and quantitative analysis of anthocyanin substances was conducted using high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) on the fruits of four development stages of 'Benihoppe' strawberry: White (WF), initial ripening (IR), part ripening (PR), and full red fruits (FR). The expression level of structural genes in anthocyanin biosynthesis pathway in different development processes was determined to provide a theoretical basis for anthocyanin metabolism and color quality regulation of strawberry fruit. The results showed 24 anthocyanins and four proanthocyanidins in 'Benihoppe' strawberry. With the ripening of the fruit, the total anthocyanin and proanthocyanidin contents gradually increased and decreased, respectively. Pelargonidin and cyanidin were the main differential metabolites in different developmental stages. Among them, pelargonidin-3-O-glucoside, pelargonidin-3-O-rutinoside and cyanidin-3-O-glucoside were the main substances in anthocyanins, accounting for 79.7%, 14.1% and 3.56% of the total anthocyanins at the full maturity of 'Benihoppe' strawberry, respectively. Real-time PCR analysis showed that the transcription levels of the anthocyanin synthesis pathway genes FaPAL1, FaC4H, FaF3H, FaANS, FaUFGT, and FaMYB10 gradually increased with the fruit development. In summary, the anthocyanin content of 'Benihoppe' strawberry fruit gradually accumulates with fruit maturity, and the substances of anthocyanin differential metabolism include pelargonidin and cyanidin. The expression level of structural genes related to anthocyanin synthesis pathway affects the anthocyanin accumulation of fruit.
- 'Benihoppe' strawberry,
- anthocyanins,
- metabolites,
- genes

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References (39)

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