Inhibition of Myristic Acid on Suilysin and the Molecular Mechanism

WANG Guizhen; LIU Hongtao; YANG Xiubai; JIANG Yuqing; YANG Lili; QIU Jiazhang

doi:10.13386/j.issn1002-0306.2022080344

Volume 44 Issue 15

Jul. 2023

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Science and Technology of Food Industry > 2023 > 44(15): 62-68. > DOI: 10.13386/j.issn1002-0306.2022080344

WANG Guizhen, LIU Hongtao, YANG Xiubai, et al. Inhibition of Myristic Acid on Suilysin and the Molecular Mechanism[J]. Science and Technology of Food Industry, 2023, 44(15): 62−68. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080344.

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Inhibition of Myristic Acid on Suilysin and the Molecular Mechanism

1.
College of Veterinary Medicine, Jilin University, Changchun 130062, China
2.
College of Biological and Food Engineering, Jilin Engineering Normal University, Changchun 130052, China

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Received Date: September 04, 2022
Available Online: June 05, 2023

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Abstract

Abstract

To explore the inhibitory effect of myristic acid against suilysin biological activity and the interactive mechanism, pore-forming assay, oligomerization assay, molecular docking, molecular dynamics simulation, minimal inhibitory concentration assay and lactate dehydrogenase activity assays were performed. The results showed that the formation of suilysin oligomer significant decreased when the concentration of myristic acid was 16 μg/mL, thereby inhibiting the pore-forming activity of suilysin, the release of hemoglobin was 8.88% of the control group, and the inhibition rate reached 91.12%. The results of molecular docking and molecular dynamics simulation showed that myristic bound to the junction of domain one, two and three, and 82Asn, 83Asn, 84Ser, 87Ile, 88Ala, 90Ile, 193Phe, 194Gly, 275Phe, 372Ile, 373Leu, 374Ser contributed higher energy which were the critical residues during the binding. Myristic did not showed anti-Streptococcus suis character (MIC values was 128 μg/mL), and the release of lactic dehydrogenase in suilysin or Streptococcus suis treatment group was 67.84% and 45.72% of the control group when the cells received 16 μg/mL myristic treatment. Taken together, myristic inhibited the pore-forming of suilysin by affecting the formation of its oligomer based on a direct binding and protected cells from suilysin or Streptococcus suis cytotoxicity which promised it a candidate for developing anti-Streptococcus suis infection drug.
- suilysin,
- molecular simulation,
- myristic acid,
- cytotoxicity

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References (34)

References

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