Analysis of Differential Metabolites between NFC and FC Bayberry Juice Based on UPLC-QTOF-MS

SUN Ruiyang; XUAN Xiaoting; CUI Yan; LIN Xudong; SHAO Xingfeng; DENG Wenyi; LING Jiangang

doi:10.13386/j.issn1002-0306.2022080132

Volume 44 Issue 15

Jul. 2023

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Science and Technology of Food Industry > 2023 > 44(15): 275-282. > DOI: 10.13386/j.issn1002-0306.2022080132

SUN Ruiyang, XUAN Xiaoting, CUI Yan, et al. Analysis of Differential Metabolites between NFC and FC Bayberry Juice Based on UPLC-QTOF-MS[J]. Science and Technology of Food Industry, 2023, 44(15): 275−282. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080132.

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Analysis of Differential Metabolites between NFC and FC Bayberry Juice Based on UPLC-QTOF-MS

SUN Ruiyang^1,,
XUAN Xiaoting^{2, 3},
CUI Yan^{2, 3},
LIN Xudong^{2, 3},
SHAO Xingfeng¹,
DENG Wenyi⁴,
LING Jiangang^{2, 3, ,}

1.
College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315000, China
2.
Institute of Agricultural Products Processing, Ningbo Academy of Agricultural Sciences, Ningbo 315000, China
3.
National Vegetable Processing Technology Research and Development Center, Ningbo 315000, China
4.
Hunan Yipindongfang Biotechnology Co., Ltd., Changsha 410000, China

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Received Date: August 14, 2022
Available Online: June 05, 2023

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Abstract

Abstract

This study analyzed the differential composition between from concentrate (FC) and not from concentrate (NFC) bayberry juices by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). NFC and FC bayberry juice were distinguished from each other by orthogonal partial least squares discriminant analysis, and 9 compounds that significantly differed in abundance between them were selected and identified according to the variable importance in the projection and the fold-change. By comparison, the content of antioxidant active components such as 4-sulfobenzoate, 5-methyltetrahydrofolic acid, cyanidin, daphnoretin, xanthotoxol and isoxanthopterin in FC bayberry juice were relatively low, whereas the bitter substances (neohesperidin, hesperidin and quercetin-3-O-neohesperidin) were relatively high. Metabolic pathways were analyzed on account of differential metabolites and five metabolic pathways with great differences were identified, among which phenylalanine metabolism, biosynthesis of flavonoids and flavonols were the key metabolic pathways of different metabolites in NFC and FC bayberry juice. This study revealed the difference of metabolites between NFC and FC bayberry juice, which provided theoretical reference for the processing and identification of NFC bayberry juice.

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