Determination of Thymol in Honey by Ultra Performance Liquid Chromatography with Fluorescence Detector

QIAN Zhenjie; ZENG Xi; CHEN Jing; ZHAO Yue; YOU Shuzhu; HE Yongxin; FENG Jiawang

doi:10.13386/j.issn1002-0306.2022070302

Volume 43 Issue 23

Nov. 2022

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Science and Technology of Food Industry > 2022 > 43(23): 328-333. > DOI: 10.13386/j.issn1002-0306.2022070302

QIAN Zhenjie, ZENG Xi, CHEN Jing, et al. Determination of Thymol in Honey by Ultra Performance Liquid Chromatography with Fluorescence Detector[J]. Science and Technology of Food Industry, 2022, 43(23): 328−333. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070302.

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Determination of Thymol in Honey by Ultra Performance Liquid Chromatography with Fluorescence Detector

1.
Guangzhou Institute for Food Inspection, Guangzhou 511400, China
2.
Gongbei Customs Technology Center, Zhuhai 519000, China

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Received Date: July 31, 2022
Available Online: October 08, 2022

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Abstract

Abstract

The presented method was developed to determine the concentration of thymol residues in honey by ultra performance liquid chromatography with a fluorescence detector. The thymol in honey was extracted by 40% acetonitrile-water solution. After centrifugation and filtration, the separation of thymol was performed on an Accucore aQ liquid chromatography column (150 mm×2.1 mm, 2.6 μm) with the gradient elution of acetonitrile and water (containing 0.1% formic acid) as mobile phases, and quantitated by external standard method with fluoresce detector (excitation wavelength 274 nm, emission wavelength 297 nm). A good linear relationship (R²=0.9999) was observed with concentration ranging from 0.01 μg/mL to 3.00 μg/mL. The average recovery of thymol was from 87.4% to 106.9%, the coefficient of variation was between 0.7% and 7.3%, and the limit of quantification was 0.10 mg/kg. The developed method was applied to determine residual thymol in 48 commercial honey samples, and the thylmol was only detected in one sample. The presented method is simple, quick, reliable, and suitable to be used for batch screening and quantitative determination of residual thymol in honey.
- honey,
- thymol,
- ultra performance liquid chromatography,
- fluorescence method,
- residue

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References (33)

References

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