Optimization of Decolorization and Deproteinization of Balanophora japonica Makino Polysaccharide by Response Surface Methodology

To study the decolorization and deproteinization conditions of Balanophora japonica polysaccharide by hydrogen peroxide and Sevag method, and to optimize the process. Based on single factor experiments, the response surface methodology was used to design a three factor and three level experiments to optimize the decolorization conditions of Balanophora japonica polysaccharides using the decolorization rate of polysaccharides as an index. Using protein removal rate and polysaccharide retention rate as indicators, a response surface experiment was designed at the factor level of Sevag reagent ratio (chloroform:n-butanol), sample solution: Sevag reagent and oscillation time to obtain the optimal process conditions. Results showed that, under the condition of pH8, the optimal decolorization process was as follows: The dosage of hydrogen peroxide was 20%, the decolorization time was 46 min, and the decolorization temperature was 60 ℃. Under this condition, the decolorization rate of polysaccharides was 84.21%. The optimal deproteinization conditions were chloroform:n-butanol 5:1, sample solution:Sevag reagent 4:1, and shock time 15 min, under this condition, the polysaccharide retention rate was 81.03%, and the protein removal rate was 40.44%. The decolorization and deproteinization of Balanophora japonica polysaccharide by hydrogen peroxide and Sevag method was stable, feasible and simple, and would be suitable for decolorization and deproteinization of Balanophora japonica polysaccharide.