Enzyme Catalytic Synthesis of Phosphatidylserine in Aqueous Phase through Immobilization of Phosphatidylcholine

ZHU Shuai; HUANG Ao; LIU Xiazhong; LIU Yun

doi:10.13386/j.issn1002-0306.2022060070

Volume 44 Issue 3

Jan. 2023

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Science and Technology of Food Industry > 2023 > 44(3): 248-254. > DOI: 10.13386/j.issn1002-0306.2022060070

ZHU Shuai, HUANG Ao, LIU Xiazhong, et al. Enzyme Catalytic Synthesis of Phosphatidylserine in Aqueous Phase through Immobilization of Phosphatidylcholine[J]. Science and Technology of Food Industry, 2023, 44(3): 248−254. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022060070.

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Enzyme Catalytic Synthesis of Phosphatidylserine in Aqueous Phase through Immobilization of Phosphatidylcholine

1.
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
2.
Hebei Meiyesiwei Biotechnology Ltd. Co., Baoding 072650, China

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Received Date: June 09, 2022
Available Online: November 28, 2022

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Abstract

Abstract

This work reported a novel technology on bioconversion of phosphatidylcholine (PC) into phosphatidylserine (PS) by phospholipase D (PLD) in an aqueous phase catalytic system through immobilization of substrate phosphatidylcholine (PC). The major factors affecting PC adsorption efficiency were investigated to obtain the optimal parameters, such as the kinds of precipitant reagents, carrier types, and substrate loading concentration. Furthermore, the time kinetics of PS catalytic synthesis by PLD through immobilization of substrate PC and the recycling utilization of enzyme PLD were also examined. Results showed that the yield of PS achieved 98.74% after 24 h reaction, and PLD had an excellent recycling utilization. When it was reused for 10 batches, PLD could still yield more than 60% of PS. In the end, the efficiency of PS catalytic synthesis by PLD was also compared between through immobilization of substrate PC and in two-liquid phase system. It was found that higher yield of PS and more excellent stability of PLD were achieved through immobilization of substrate PC than in two-liquid phase system. These findings would provide a novel catalytic system for PC bioconversion into PS in the future.
- phosphatidylcholine (PC),
- phosphatidylserine (PS),
- enzymatic modification,
- immobilization of substrate,
- two-liquid phase system

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References

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