Study on the Mechanism of Whey Protein Isolate Masking Bitterness of <i>Zanthoxylum bungeanum</i> Maxim.

KE Jingxuan; LI Yongxiu; LI Jie; LI Mi; MA Yanli; ZHANG Zhiqing

doi:10.13386/j.issn1002-0306.2022050255

Volume 44 Issue 19

Oct. 2023

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Science and Technology of Food Industry > 2023 > 44(19): 10-16. > DOI: 10.13386/j.issn1002-0306.2022050255

KE Jingxuan, LI Yongxiu, LI Jie, et al. Study on the Mechanism of Whey Protein Isolate Masking Bitterness of Zanthoxylum bungeanum Maxim. DOI: 10.13386/j.issn1002-0306.2022050255

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KE Jingxuan, LI Yongxiu, LI Jie, et al. Study on the Mechanism of Whey Protein Isolate Masking Bitterness of Zanthoxylum bungeanum Maxim. DOI: 10.13386/j.issn1002-0306.2022050255

Citation:

KE Jingxuan, LI Yongxiu, LI Jie, et al. Study on the Mechanism of Whey Protein Isolate Masking Bitterness of Zanthoxylum bungeanum Maxim. DOI: 10.13386/j.issn1002-0306.2022050255

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Study on the Mechanism of Whey Protein Isolate Masking Bitterness of Zanthoxylum bungeanum Maxim.

KE Jingxuan^{1, 2,},
LI Yongxiu²,
LI Jie¹,
LI Mi¹,
MA Yanli¹,
ZHANG Zhiqing^2, ,

1.
Zhang Zhongjing School of Chinese Medicine, Nanyang Institute of Technology, Nanyang 473004, China
2.
College of Food Science, Sichuan Agricultural University, Ya'an 625014, China

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Received Date: May 22, 2022
Available Online: July 21, 2023

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Abstract

Abstract

Zanthoxylum bungeanum Maxim. is an essential spice and medicinal plant in China. Zanthoxylum bungeanum Maxim. has a distinct bitterness due to 7-methoxylcoumarin. The bitterness of Zanthoxylum bungeanum Maxim. restricted the development and utilization of its products. This study aimed to explore the masking effect and mechanism of whey protein isolate on the bitterness of Zanthoxylum bungeanum Maxim.. The masking mechanism of the bitterness of Zanthoxylum bungeanum Maxim. by whey protein isolate was investigated using the method of sensory evaluation, electronic tongue and molecular docking technique with Zanthoxylum bungeanum Maxim. oleoresin as the matrix. The results indicated that whey protein isolate could significantly mask the bitterness of Zanthoxylum bungeanum Maxim. oleoresin. Furthermore, there was a mass concentration-effect relationship between bitterness reduction values of Zanthoxylum bungeanum Maxim. oleoresin and the additive amount of whey protein isolate. When the dosage of whey protein isolate was 1.00% and the concentration of Zanthoxylum bungeanum oleoresin was 0.5%, the reduction value of ΔBI was 1.8, indicating that the bitterness masking effect was good. The molecular docking model showed that 7-methoxycoumarin and β-lactoglobulin had 20 docking conformations in cluster 2, and the lowest binding free energy was −5.90 kcal/mol. Hence, cluster 2 was the most likely binding region for their interaction. It could be concluded that whey protein isolate could mask the bitterness of Zanthoxylum bungeanum Maxim. oleoresin by binding to 7-methoxycoumarin and preventing it from binding to bitter receptors. The results may provide a reference for the taste correction of Zanthoxylum bungeanum Maxim. products.
- Zanthoxylum bungeanum Maxim.,
- bitterness masking,
- whey protein isolate,
- molecular docking

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References (33)

References

[1]	课净璇, 瞿瑗, 黎杉珊, 等. 基于GC-MS建立花椒挥发油指纹图谱及在汉源红花椒鉴定中的应用[J]. 中国粮油学报,2018,33(11):117−125. [KE J X, QU Y, LI S S, et al. Establishment of GC-MS fingerprint based on essential oil components in Zanthoxylum and application on hanyaun Zanthoxylum bungeanum[J]. Journal of the Chinese Cereals and Oils,2018,33(11):117−125. KE J X, QU Y, LI S S, et al. Establishment of GC-MS fingerprint based on essential oil components in Zanthoxylum and application on hanyaun Zanthoxylum bungeanum[J]. Journal of the Chinese Cereals and Oils, 2018, 33(11): 117-125.
[2]	QU Y, HOU X, KE J, et al. Mechanistic investigation on binding interaction of konjac glucomannan with Zanthoxylum armatum DC. seed glutelin in solution[J]. International Journal of Biological Macromolecules,2019,123:308−313. doi: 10.1016/j.ijbiomac.2018.11.094
[3]	课净璇, 黎杉珊, 申光辉, 等. 基于双指标分析法和聚类分析法的花椒红外指纹图谱研究[J]. 食品与机械,2017,33(3):55−61. [KE J X, LI S S, SHEN G H, et al. Infrared fingerprint analysis of Zanthoxylum based on sequential dual-indexes and cluster analysis method[J]. Food and Machinery,2017,33(3):55−61. KE J X, LI S S, SHEN G H, et al. Infrared fingerprint analysis of Zanthoxylum based on sequential dual-indexes and cluster analysis method[J]. Food and Machinery, 2017, 33(3): 55-61.
[4]	KE J X, QU Y, LI S S, et al. Application of HPLC fingerprint based on acid amide components in Chinese prickly ash (Zanthoxylum)[J]. Industrial Crops and Products,2018,119:267−276. doi: 10.1016/j.indcrop.2018.04.018
[5]	KE J X, RAO L, ZHOU L, et al. Non-destructive determination of volatile oil and moisture content and discrimination of geographical origins of Zanthoxylum bungeanum Maxim. by hyperspectral imaging[J]. Infrared Physics & Technology,2020,105:103185.
[6]	KE J X, CHENG J, LUO Q, et al. Identification of two bitter components in Zanthoxylum bungeanum Maxim. and exploration of their bitter taste mechanism through receptor hTAS2R14[J]. Food Chemistry,2021,338:127816. doi: 10.1016/j.foodchem.2020.127816
[7]	冯宝龙, 任海斌, 段佳慧, 等. 天然苦味分子识别及苦味阈值预测模型[J]. 食品工业科技,2022,43(4):24−32. [FENG B L, REN H B, DUAN X H, et al. Molecular recognition and threshold prediction model of bitterness in natural compounds[J]. Science and Technology of Food Industry,2022,43(4):24−32. FENG B L, REN H B, DUAN X H, et al. Molecular recognition and threshold prediction model of bitterness in natural compounds[J]. Science and Technology of Food Industry, 2022, 43(4): 24-32.
[8]	WISE P M, DAMANI S, BRESLIN P A S. Sodium, but not potassium, blocks bitterness in simple model chicken broths[J]. Journal of Food Science and Technology,2019,56(6):3151−3156. doi: 10.1007/s13197-019-03770-1
[9]	BECK T K, JENSEN S, BJOERN G K, et al. The masking effect of sucrose on perception of bitter compounds in brassica vegetables[J]. Journal of Sensory Studies,2014,29(3):190−200. doi: 10.1111/joss.12094
[10]	ZHANG L, PETERSON D G. Identification of bitter compounds in extruded corn puffed products[J]. Food Chemistry,2018,254:185−192. doi: 10.1016/j.foodchem.2018.01.161
[11]	KOLA O, KAYA C, DURAN H, et al. Removal of limonin bitterness by treatment of ion exchange and adsorbent resins[J]. Food Science and Biotechnology,2010,19(2):411−416. doi: 10.1007/s10068-010-0058-2
[12]	娄潇雨, 童群义. 超声波辅助碱液-酶解法对柚皮脱苦效果的研究[J]. 食品工业科技,2017,38(13):206−211. [LOU X Y, TONG Q Y. Study on effect of debittering shaddock peel by ultrasound-assisted alkaline solution-enzymolysis method[J]. Science and Technology of Food Industry,2017,38(13):206−211. LOU X Y, TONG Q Y. Study on effect of debittering shaddock peel by ultrasound-assisted alkaline solution-enzymolysis method[J]. Science and Technology of Food Industry, 2017, 38(13): 206-211.
[13]	李学林, 仇继玺, 刘瑞新, 等. 苦味抑制剂的研究进展[J]. 中国实验方剂学杂志,2012,18(21):335−338. [LI X L, CHOU J X, LIU R X, et al. Recent advances of bitterness suppressants[J]. Chinese Journal of Experimental Traditional Medical Formulae,2012,18(21):335−338. LI X L, CHOU J X, LIU R X, et al. Recent advances of bitterness suppressants[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18(21): 335-338.
[14]	晏敏, 周宇, 贺肖寒, 等. 柑橘籽中柠檬苦素及类似物的生物活性研究进展[J]. 食品与发酵工业,2018,44(2):290−296. [YAN M, ZHOU Y, HE X H, et al. Research progress on the bioactivity of limonin and its analogues in citrus[J]. Food and Fermentation Industries,2018,44(2):290−296. YAN M, ZHOU Y, HE X H, et al. Research progress on the bioactivity of limonin and its analogues in citrus[J]. Food and Fermentation Industries, 2018, 44( 2): 290-296.
[15]	任建敏, 吴四维. 功能食品苦味功能因子生理活性及其掩苦技术的研究进展[J]. 食品工业科技,2013,3:396−400. [REN J M, WU S W. Research progress in bioactivities of the bitter compounds in functional foods and their bitter taste masking technology[J]. Science and Technology of Food Industry,2013,3:396−400. REN J M, WU S W. Research progress in bioactivities of the bitter compounds in functional foods and their bitter taste masking technology[J]. Science and Technology of Food Industry, 2013, 3: 396-400.
[16]	FONTOIN H, SAUCIER C, TEISSEDRE P L, et al. Effect of pH, ethanol and acidity on astringency and bitterness of grape seed tannin oligomers in model wine solution[J]. Food Quality and Preference,2008,19(3):286−291. doi: 10.1016/j.foodqual.2007.08.004
[17]	SITI RASHIMA R, MAIZURA M, KANG W M, et al. Influence of sodium chloride treatment and polysaccharides as debittering agent on the physicochemical properties, antioxidant capacity and sensory characteristics of bitter gourd (Momordica charantia) juice[J]. Journal of Food Science and Technology,2017,54(1):228−235. doi: 10.1007/s13197-016-2454-y
[18]	王知非, 林璐, 孙伟峰, 等. 苦味肽和苦味受体研究进展[J]. 中国调味品,2016,41(9):152−156. [WANG Z F, LIN L, SUN W F, et al. Research progress of bitter peptides and bitter receptors[J]. China Condiment,2016,41(9):152−156. WANG Z F, LIN L, SUN W F, et al. Research progress of bitter peptides and bitter receptors[J]. China Condiment, 2016, 41(9): 152-156.
[19]	胡德美, 郑霏艳, 尹文凤, 等. 矫味剂在掩盖中药苦味中的应用[J]. 云南中医中药杂志,2017,38(10):86−88. [HU D M, ZHENG F Y, YIN W F, et al. Application of flavoring agent in masking the bitterness of Traditional Chinese medicine[J]. Yunnan Journal of Traditional Chinese Medicine,2017,38(10):86−88. HU D M, ZHENG F Y, YIN W F, et al. Application of flavoring agent in masking the bitterness of Traditional Chinese medicine[J]. Yunnan Journal of Traditional Chinese Medicine, 2017, 38(10): 86-88.
[20]	SHARAFI M, HAYES J E, DUFFY V B. Masking vegetable bitterness to improve palatability depends on vegetable type and taste phenotype[J]. Chemosensory Perception,2013,6(1):8−19. doi: 10.1007/s12078-012-9137-5
[21]	FENG Y, YUAN D, CAO C, et al. Changes of in vitro digestion rate and antioxidant activity of digestion products of ethanol-modified whey protein isolates[J]. Food Hydrocolloids,2022,131:107756. doi: 10.1016/j.foodhyd.2022.107756
[22]	王伟江, 郑建仙. 天然苦味抑制剂的研究与应用[J]. 中国调味品,2006,2:13−16. [WANG W J, ZHENG J X. Research and application of natural bitterness Inhibitors[J]. China Condiment,2006,2:13−16. WANG W J, ZHENG J X. Research and application of natural bitterness Inhibitors[J]. China Condiment, 2006, 2: 13-16.
[23]	HOMMA R, YAMASHITA H, FUNAKI J, et al. Identification of bitterness-masking compounds from cheese[J]. Journal of Agricultural and Food Chemistry,2012,60(18):4492−4499. doi: 10.1021/jf300563n
[24]	TENNEY K, HAYES J, EUSTON S, et al. Binding of caffeine and quinine by whey protein and the effect on bitterness[J]. Journal of Food Science,2017,82(2):509−516. doi: 10.1111/1750-3841.13588
[25]	ALEXANDER L, DE BEER D, MULLER M, et al. Bitter profiling of phenolic fractions of green Cyclopia genistoides herbal tea[J]. Food Chemistry,2019,276:626−635. doi: 10.1016/j.foodchem.2018.10.030
[26]	OMUR-OZBEK P, DIETRICH A M. Developing hexanal as an odor reference standard for sensory analysis of drinking water[J]. Water Research,2008,42(10−11):2598−2604. doi: 10.1016/j.watres.2008.01.010
[27]	李学林, 田亮玉, 张耀, 等. 4种苦味抑制剂对3种苦味成分的掩味效果[J]. 中成药,2018,40(8):1741−1747. [LI X L, TIAN L Y, ZHANG Y, et al. Taste-masking effects of four bitterness inhibitors on three bitter constituents[J]. Chinese Traditional Patent Medicine,2018,40(8):1741−1747. LI X L, TIAN L Y, ZHANG Y, et al. Taste-masking effects of four bitterness inhibitors on three bitter constituents[J]. Chinese Traditional Patent Medicine, 2018, 40(8): 1741-1747.
[28]	ITO M, IKEHAMA K, YOSHIDA K, et al. Bitterness prediction of H1-antihistamines and prediction of masking effects of artificial sweeteners using an electronic tongue[J]. International Journal of Pharmaceutics,2013,441(1−2):121−127. doi: 10.1016/j.ijpharm.2012.11.047
[29]	LI X, FENG C, HONG H, et al. Novel ACE inhibitory peptides derived from whey protein hydrolysates: Identification and molecular docking analysis[J]. Food Bioscience,2022,48:101737. doi: 10.1016/j.fbio.2022.101737
[30]	包敏, 钮成拓, 陈玙捷, 等. 分子对接和定点突变提高内切菊粉酶的催化活力[J]. 食品与发酵工业,2021,47(19):57−62. [BAO M, NIU C T, CHEN Y J, et al. Molecular docking and site-directed mutagenesis of endo-inulinase to improve catalytic activity[J]. Food and Fermentation Industries,2021,47(19):57−62. BAO M, NIU C T, CHEN Y J, et al. Molecular docking and site-directed mutagenesis of endo-inulinase to improve catalytic activity[J]. Food and Fermentation Industries, 2021, 47(19): 57-62.
[31]	阮仕洋, 陈慧, 曾凡丽, 等. 基于网络药理学和分子对接探讨桑枝改善高尿酸血症的作用机制[J]. 食品工业科技,2022,43(19):23−30. [RUN S Y, CHEN H, ZENG F L, et al. Study on the mechanism of mulberry twig improving hyperuricemia based onnetwork pharmacology and molecular docking[J]. Science and Technology of Food Industry,2022,43(19):23−30. RUN S Y, CHEN H, ZENG F L, et al. Study on the mechanism of mulberry twig improving hyperuricemia based onnetwork pharmacology and molecular docking[J]. Science and Technology of Food Industry, 2022, 43(19): 23-30.
[32]	赵志恒, 毕经会, 叶诗洁, 等. 基于网络药理学和分子对接探究黄芪-女贞子治疗免疫缺陷病的作用机制[J]. 食品工业科技,2022,43(3):374−383. [ZHAO Z H, BI J H, YE S J, et al. Mechanism of Astragalus-Ligustrum lucidum in the treatment of immunodeficiency diseases based on network pharmacology and molecular docking[J]. Science and Technology of Food Industry,2022,43(3):374−383. ZHAO Z H, BI J H, YE S J, et al. Mechanism of Astragalus-Ligustrum lucidum in the treatment of immunodeficiency diseases based on network pharmacology and molecular docking[J]. Science and Technology of Food Industry, 2022, 43(3): 374-383.
[33]	BINELLO A, CRAVOTTO G, NANO G M, et al. Synthesis of chitosan-cyclodextrin adducts and evaluation of their bitter-masking properties[J]. Flavour and Fragrance Journal,2004,19(5):394−400. doi: 10.1002/ffj.1434

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