Identification and Screening of Bacteriocin-producing <i>Lactobacillus plantarum</i> and Its Effects on Preservation of Large Yellow Croaker

XU Huadi; GAO Tengqi; CUI Zihang; LIU Chunsheng; PAN Saikun; YANG Jie

doi:10.13386/j.issn1002-0306.2022050162

Volume 43 Issue 21

Oct. 2022

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Science and Technology of Food Industry > 2022 > 43(21): 9-19. > DOI: 10.13386/j.issn1002-0306.2022050162

XU Huadi, GAO Tengqi, CUI Zihang, et al. Identification and Screening of Bacteriocin-producing Lactobacillus plantarum and Its Effects on Preservation of Large Yellow Croaker[J]. Science and Technology of Food Industry, 2022, 43(21): 9−19. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022050162.

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Identification and Screening of Bacteriocin-producing Lactobacillus plantarum and Its Effects on Preservation of Large Yellow Croaker

College of Food Science and Engineering, Jiangsu Ocean University, Lianyungang 222005, China

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Received Date: May 16, 2022
Available Online: August 25, 2022

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Abstract

Abstract

In this study, the screening of lactic acid bacteria from traditional fermented food was carried out by enrichment culture, dilution separation, and agar plate diffusion experiments. Physiological and biochemical and 16S rDNA gene sequence homology analysis were used to identify the lactic acid bacteria. Then, the main antibacterial substances produced by the lactic acid bacteria were determined by acid excretion, hydrogen peroxide exclusion, and protease hydrolysis. Finally, the bacteriocin crude extract was obtained by alcohol precipitation and Sephadex LH-20 chromatography, and then was applied to the preserving the whole large yellow croaker. To evaluate the preservation effect of the bacteriocin crude extract on the large yellow croaker, sensory evaluation, pH determination, viable cell counts, TVB-N determination and texture profile analysis were systematically carried out every 3 days. The experimental results showed that the lactic acid bacteria isolated from shrimp paste was Lactobacillus plantarum and named MMB-11. Meanwhile, the bacteriocin produced by MMB-11 was sensitive to protease and had notable acid tolerance and thermal stability. With the application of the bacteriocin on the preservation of large yellow croaker, the viable cell counts in MMB-11 group, control group and Nisin-treated group after 12 d of storage were 3.50×10⁴, 1.10×10⁵ and 4.50×10⁴ CFU/g, respectively. Furthermore, after 12 d of storage, the TVB-N treated with MMB-11 group, control group and Nisin group were 20.91±0.66、31.37±0.21 and 21.44±0.45 mg/100 g, respectively. Overall, the results showed that the crude bacteriocin extract of MMB-11 had a better preservation effect on large yellow croaker than the Nisin-treated group and control group. This study provides a fundamental knowledge for developing safe and harmless biological preservatives for large yellow croaker.
- Lactobacillus plantarum,
- isolation and identification,
- bacteriocin,
- large yellow croaker,
- preservation

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[1]	刘家富. 大黄鱼养殖与生物学[M]. 厦门大学出版社, 2013: 1−8 LIU Jiafu. Culture and biology of large yellow croaker[M]. Xiamen University Press, 2013: 1−8.
[2]	郭全友, 邢晓亮, 姜朝军, 等. 野生和养殖大黄鱼(Larimchthys crocea)品质特征与差异性探究[J]. 现代食品科技,2019,35(10):92−101. [GUO Quanyou, XING Xiaoliang, JIANG Chaojun, et al. Quality characteristics and differences between wild and cultured large yellow croaker (Larimchthys crocea)[J]. Modern Food Science and Technology,2019,35(10):92−101.
[3]	FU L L, WANG C, RUAN X M, et al. Preservation of large yellow croaker (Pseudosciaena crocea) by Coagulin L1208, a novel bacteriocin produced by Bacillus coagulans L1208[J]. International Journal of Food Microbiology,2018,266:60−68. doi: 10.1016/j.ijfoodmicro.2017.11.012
[4]	郭本恒. 益生菌[M]. 北京: 化学工业出版社, 2003: 3−5 GUO Benheng. Probiotics[M]. Beijing: Chemical Industry Press, 2003: 3−5.
[5]	马欢欢. 海鱼肠道中拮抗性乳酸菌筛选及在大菱鲆保鲜中的应用[D]. 锦州: 渤海大学, 2017 MA Huanhuan. Screening antagonistic lactic acid bacteria from intestinal tract of Marine fish and its application in turbot preservation[D]. Jinzhou: Bohai University, 2017.
[6]	GALVEZ A, ABRIOUEL H, LOPEZ R L, et al. Bacteriocin-based strategies for food biopreservation[J]. International Jurnal of Food Microbiology,2007,120(1-2):51−70. doi: 10.1016/j.ijfoodmicro.2007.06.001
[7]	黄晓英, 李启明, 吴华星, 等. 传统发酵食品中具有抑菌活性乳酸菌筛选及其代谢产物稳定性分析[J]. 现代食品科技,2021,37(11):68−78. [HUANG Xiaoying, LI Qiming, WU Huaxing, et al. Screening of lactic acid bacteria with antibacterial activity and stability analysis of their metabolites in traditional fermented food[J]. Modern Food Science and Technology,2021,37(11):68−78. doi: 10.13982/j.mfst.1673-9078.2021.11.0423
[8]	万倩, 李启明, 吴华星, 等. 传统发酵食品中乳酸菌的安全性评估[J]. 现代食品科技,2021,37(6):276−286. [WAN Qian, LI Qiming, WU Huaxing, et al. Safety evaluation of lactic acid bacteria in traditional fermented food[J]. Modern Food Science and Technology,2021,37(6):276−286. doi: 10.13982/j.mfst.1673-9078.2021.6.1057
[9]	JITPAKDEE J, KANTACHOTE D, KANZAKI H, et al. Selected probiotic lactic acid bacteria isolated from fermented foods for functional milk production: Lower cholesterol with more beneficial compounds[J]. LWT,2021,135:110061. doi: 10.1016/j.lwt.2020.110061
[10]	聂紫玉, 吴艳阳, 王增光, 等. 植物源益生乳酸菌的筛选及其特性[J/OL]. 食品科学: 1−14. http://kns.cnki.net/kcms/detail/11.2206.TS.20211221.1221.012.html. NIE Ziyu, WU Yanyang, WANG Zengguang, et al. Screening and characterization of plant-derived probiotic lactic acid bacteria[J]. Food Science: 1−14.
[11]	赵圣明, 赵岩岩, 马汉军. 植物乳杆菌JLA-9产细菌素的分离纯化[J]. 食品与发酵工业,2017,43(6):60−65. [ZHAO Shengming, ZHAO Yanyan, MA Hanjun. Isolation and purification of bacteriocin from Lactobacillus plantarum JLA-9[J]. Food and Fermentation Industries,2017,43(6):60−65. doi: 10.13995/j.cnki.11-1802/ts.201706010
[12]	马国涵, 马欢欢, 吕欣然, 等. 大菱鲆肠道中广谱拮抗活性乳酸菌的筛选及其细菌素鉴定[J]. 食品科学,2019,40(6):159−165. [MA Guohan, MA Huanhuan, LÜ Xinran, et al. Screening for broad-spectrum antagonistic lactic acid bacteria from intestine of turbot and identification of bacteriocin produced by it[J]. Food Science,2019,40(6):159−165. doi: 10.7506/spkx1002-6630-20180325-332
[13]	曾维友, 周於强, 池浩. 泡菜中乳酸菌的分离鉴定及抗性筛选[J]. 中国酿造,2021,40(10):163−167. [ZENG Weiyou, ZHOU Yuqiang, CHI Hao. Isolation, identification and resistance screening of lactic acid bacteria from pickles[J]. China Brewing,2021,40(10):163−167. doi: 10.11882/j.issn.0254-5071.2021.10.027
[14]	DING W R, SHI C, CHEN M, et al. Screening for lactic acid bacteria in traditional fermented Tibetan yak milk and evaluating their probiotic and cholesterol-lowering potentials in rats fed a high-cholesterol diet[J]. Journal of Functional Foods,2017,32:324−332. doi: 10.1016/j.jff.2017.03.021
[15]	罗林根, 穰杰, 夏立秋. 遗传改造乳酸链球菌合成的研究进展[J]. 湖南师范大学自然科学学报,2021,44(5):102−107. [LUO Lingen, RANG Jie, XIA Liqiu. Synthesis of Nisin by genetic modification of Streptococcus lactis[J]. Journal of Natural Science of Hunan Normal University,2021,44(5):102−107.
[16]	ONG J S, TAYLOR T D, WONG C B, et al. Extracellular transglycosylase and glyceraldehyde-3-phosphate dehydrogenase attributed to the anti-staphylococcal activity of Lactobacillus plantarum USM8613[J]. Journal of Biotechnology,2019,300:20−31. doi: 10.1016/j.jbiotec.2019.05.006
[17]	PEI J J, JIN W G, ABD EL-ATY A M, et al. Isolation, purification, and structural identification of a new bacteriocin made by Lactobacillus plantarum found in conventional kombucha[J]. Food Control,2020,110:106923. doi: 10.1016/j.foodcont.2019.106923
[18]	SUMA K, MISRA M C, VARADARAJ M C. Plantaricin LP84, a broad spectrum heat-stable bacteriocin of Lactobacillus plantarum NCIM 2084 produced in a simple glucose broth medium[J]. International Journal of Food Microbiology,1998,40(1-2):17−25. doi: 10.1016/S0168-1605(98)00010-5
[19]	GOLNESHIN A, GOR M C, WILLIAMSON N, et al. Discovery and characterisation of circular Bacteriocin plantacyclin B21AG from Lactiplantibacillus plantarum B21[J]. Heliyon,2020,6(8):e04715. doi: 10.1016/j.heliyon.2020.e04715
[20]	MUHAMMAD Z, RAMZAN R, ABDELAZEZ A, et al. Assessment of the antimicrobial potentiality and functionality of Lactobacillus plantarum strains isolated from the conventional inner mongolian fermented cheese against foodborne pathogens[J]. Pathogens,2019,8(2):2−20.
[21]	LIM S M, LEE N K, PAIK H D. Antibacterial and anticavity activity of probiotic Lactobacillus plantarum 200661 isolated from fermented foods against Streptococcus mutans[J]. LWT,2020,118:108840. doi: 10.1016/j.lwt.2019.108840
[22]	WANG N, YUAN L, FAIZAN A S, et al. Inhibitory effect of Lactobacillus plantarum metabolites against biofilm formation by bacillus licheniformis isolated from milk powder products[J]. Food Control,2019,106:106721. doi: 10.1016/j.foodcont.2019.106721
[23]	CHEN L, GU Q, LI P, et al. Purification and characterization of plantaricin ZJ316, a novel bacteriocin against Listeria monocytogenes from Lactobacillus plantarum ZJ316[J]. Journal of Food Protection,2018,81(12):1929−1935. doi: 10.4315/0362-028X.JFP-18-306
[24]	张艳霞, 谢成民, 周纷, 等. 大黄鱼保鲜技术的研究进展[J]. 食品工业科技,2019,40(13):299−303. [ZHANG Yanxia, XIE Chengmin, ZHOU Fen, et al. Research progress of preservation technology of large yellow croaker[J]. Science and Technology of Food Industry,2019,40(13):299−303. doi: 10.13386/j.issn1002-0306.2019.13.050
[25]	李婷婷. 大黄鱼生物保鲜技术及新鲜度指示蛋白研究[D]. 杭州: 浙江工商大学, 2013 LI Tingting. Study on biological preservation technology and freshness indicator protein of large yellow croaker[D]. Hangzhou: Zhejiang Gongshang University, 2013.
[26]	蓝蔚青, 胡潇予, 王倩, 等. 冰鲜大黄鱼植物源复合保鲜液的优化研究[J]. 中国食品学报,2019,19(10):206−211. [LAN Weiqing, HU Xiaoyu, WANG Qian, et al. Optimization of plant-derived compound fresh-keeping solution for chilled large yellow croaker[J]. Chinese Journal of Food Science,2019,19(10):206−211. doi: 10.16429/j.1009-7848.2019.10.025
[27]	张旭光, 李婷婷, 朱军莉, 等. 茶多酚处理对冷藏养殖大黄鱼品质的影响[J]. 茶叶科学,2011,31(2):105−111. [ZHANG Xuguang, LI Tingting, ZHU Junli, et al. Effects of tea polyphenol treatment on quality of large yellow croaker cultured in cold storage[J]. Tea science,2011,31(2):105−111. doi: 10.3969/j.issn.1000-369X.2011.02.005
[28]	王玉婷. 复合生物保鲜剂在大黄鱼保鲜中的应用研究[D]. 济南: 山东轻工业学院, 2011 WANG Yuting. Application of compound biological preservative in preservation of large yellow croaker[D]. Jinan: Shandong University of Light Industry, 2011.
[29]	陈丽娇, 郑明锋. 大黄鱼海藻酸钠涂膜保鲜效果研究[J]. 农业工程学报,2003(4):209−211. [CHEN Lijiao, ZHENG Mingfeng. Effect of sodium alginate coating on preservation of large yellow croaker[J]. Transactions of the Chinese Society of Agricultural Engineering,2003(4):209−211. doi: 10.3321/j.issn:1002-6819.2003.04.051
[30]	侯春宇, 买尔孜亚·买买提, 周纷, 等. 复配香辛料保鲜剂对大黄鱼贮藏期间品质的影响[J]. 食品工业科技,2019,40(13):221−225, 230. [HOU Chunyu, MAIERZIYA Maimaiti, ZHOU Fen, et al. Effects of compound spices preservatives on quality of large yellow croaker during storage[J]. Science and Technology of Food Industry,2019,40(13):221−225, 230. doi: 10.13386/j.issn1002-0306.2019.13.036
[31]	王蒙, 蓝蔚青, 邱泽慧, 等. 苹果多酚处理后冰鲜大黄鱼贮藏期间品质与水分迁移的变化[J]. 食品与发酵工业,2019,45(21):93−101. [WANG Meng, LAN Weiqing, QIU Zehui, et al. Changes in quality and water migration of chilled large yellow croaker after apple polyphenol treatment during storage[J]. Food and Fermentation Industries,2019,45(21):93−101. doi: 10.13995/j.cnki.11-1802/ts.021339
[32]	孟玉霞. 天然植物精油对冷藏海水养殖鱼类特定腐败菌的抑制作用及保鲜效果研究[D]. 大连: 大连海洋大学, 2018 MENG Yuxia. Inhibition and preservation effect of natural plant essential oils on specific spoilage bacteria of refrigerated mariculture fish[D]. Dalian: Dalian Ocean University, 2018.
[33]	赵钰. 凝结芽孢杆菌抑菌物质分离鉴定及其对大黄鱼保鲜效果的研究[D]. 杭州: 浙江工商大学, 2015 ZHAO Yu. Isolation and identification of bacteriostatic substances of Bacillus coagulans and their preservation effect on large yellow croaker[D]. Hangzhou: Zhejiang Gongshang University, 2015.
[34]	孙杰, 姜杰, 冯彬斌, 等. 一株产细菌素乳酸菌的筛选及其细菌素特性研究[J]. 食品与发酵工业,2018,44(9):48−52. [SUN Jie, JIANG Jie, FENG Binbin, et al. Screening and characterization of bacteriocin-producing lactic acid bacteria[J]. Food and Fermentation Industries,2018,44(9):48−52. doi: 10.13995/j.cnki.11-1802/ts.017321
[35]	高鹏. 广谱抗菌乳酸菌的分离鉴定及细菌素的提取、纯化及结构鉴定[D]. 南京: 南京农业大学, 2016 GAO Peng. Isolation and identification of broad-spectrum antibacterial lactic acid bacteria and extraction, purification and structure identification of bacteriocin[D]. Nanjing: Nanjing Agricultural University, 2016.
[36]	BUDANAN R E, GIBBONR N E. 伯杰细菌鉴定手册[M]. 北京: 科学出版社, 1984: 477−478 BUDANAN R E, GIBBONR N E. Berger handbook of bacterial identification[M]. Beijing: Science Press, 1984: 477−478.
[37]	凌代文, 东秀珠. 乳酸细菌分类鉴定及实验方法[M]. 北京: 中国轻工业出版社, 1999 LING Daiwen, DONG Xiuzhu. Classification, identification and experimental methods of lactic acid bacteria[M]. Beijing: China Light Industry Press, 1999.
[38]	刘苗, 于筛成, 张虹, 等. 中药醇沉工艺及设备浅析[J]. 中成药杂志,2007,29(8):1202−1204. [LIU Miao, YU Shaicheng, ZHANG Hong, et al. Alcohol precipitation technology and equipment of traditional Chinese medicine[J]. Chinese Journal of Patent Medicine,2007,29(8):1202−1204.
[39]	REN D Y, ZHU J W, GONG S J, et al. Antimicrobial characteristics of lactic acid bacteria isolated from homemade fermented foods[J]. BioMed Research International,2018,2018(8):1−9.
[40]	DIGAITIENE A, HANSEN Å S, JUODEIKIENE G, et al. Lactic acid bacteria isolated from rye sourdoughs produce bacteriocin-like inhibitory substances active against Bacillus subtilis and fungi[J]. Journal of Applied Microbiology,2012,112(4):732−742. doi: 10.1111/j.1365-2672.2012.05249.x
[41]	郭儒岳, 凌建刚, 叶宇飞, 等. 流化冰超冷却对养殖大黄鱼贮藏保鲜效果的影响[J]. 食品工业科技,2016,5(8):307−312. [GUO Ruyue, LING Jiangang, YE Yufei, et al. Fluidization ice super cooling effect on cultured large yellow croaker fresh-keeping effect[J]. Journal of Food Science and Technology,2016,5(8):307−312. doi: 10.13386/j.issn1002-0306.2016.08.056
[42]	陈培基, 李刘冬, 黄珂. 自动定氮仪测定水产品中的挥发性盐基氮[J]. 南方水产,2005(6):50−54. [CHEN Peiji, LI Liudong, HUANG Ke. Determination of volatile base nitrogen in aquatic products by automatic nitrogen determination apparatus[J]. Southern Fisheries,2005(6):50−54.
[43]	CHEN L H, ZENG W H, RONG Y Z, et al. Compositions, nutritional and texture quality of wild-caught andcage-cultured small yellow croaker[J]. Journal of Food Composition and Analysis,2022,107:104370. doi: 10.1016/j.jfca.2021.104370
[44]	刘佳秀, 查振兴, 于文溪, 等. 海洋源乳酸菌在黄花鱼肉保鲜中的应用研究[J]. 食品科学技术学报,2018,36(6):58−65. [LIU Jiaxiu, ZHA ZhenXing, YU Wenxi, et al. Application of marine lactic acid bacteria in the preservation of yellow flower fish[J]. Journal of Food Science and Technology,2018,36(6):58−65. doi: 10.3969/j.issn.2095-6002.2018.06.009
[45]	OLATUNDE O O, BENJAKUL S, VONGKAMJAN K. Comparative study on nitrogen and argon-based modified atmosphere packaging on microbiological, chemical, and sensory attributes as well as on microbial diversity of Asian sea bass[J]. Food Packaging and Shelf Life,2019,22:100404. doi: 10.1016/j.fpsl.2019.100404
[46]	唐智鹏, 陈晨伟, 谢晶, 等. 聚乙烯醇活性薄膜对大黄鱼保鲜效果及品质动态监控[J]. 食品工业科技,2019,40(10):290−296. [TANG Zhipeng, CHEN Chenwei, XIE Jing, et al. Preservation effect and quality dynamic monitoring of polyvinyl alcohol active film on large yellow croaker[J]. Science and Technology of Food Industry,2019,40(10):290−296. doi: 10.13386/j.issn1002-0306.2019.10.048
[47]	许萍, 黄敏, 廖涛, 等. 大蒜素复配保鲜剂对大口黑鲈保鲜效果的影响[J]. 食品科学技术学报,2021,39(4):148−155. [XU Ping, HUANG Min, LIAO Tao, et al. Effects of allicin compound preservative on preservation effect of largemouth bass[J]. Journal of Food Science and Technology,2021,39(4):148−155.
[48]	高永悦, 吕欣然, 杜宏, 等. 降解温和气单胞菌AHLs乳酸菌的筛选及在三文鱼保鲜中的应用[J]. 食品科学: 1−10 [2022-06-19]. http://kns.cnki.net/kcms/detail/11.2206.ts.20211015.2110.058.html GAO Yongyue, LU Xinran, DU Hong, et al. Screening and application of AHLs lactic acid bacteria in salmon preservation[J]. Food Science:1−10 [2022-06-19].
[49]	罗晶. Nisin抑菌稳定性研究与应用[D]. 天津: 天津科技大学, 2015 LUO Jing. Study and application of antibacterial stability of Nisin[D]. Tianjin: Tianjin University of Science and Technology, 2015.
[50]	许柳. 乳清蛋白酶解物抗氧化活性及其在冷却鸭肉保鲜中的应用研究[D]. 雅安: 四川农业大学, 2010 XU Liu. Study on antioxidant activity of whey protease hydrolysates and its application in chilled duck meat preservation[D]. Yaan: Sichuan Agricultural University, 2010.
[51]	史国萃, 常晶, 宣晓婷, 等. 海水鱼肠道低温乳酸菌的分离及其在南美白对虾保鲜中的应用研究[J]. 食品科技,2019,44(7):37−42. [SHI Guocui, CHANG Jing, XUAN Xiaoting, et al. Isolation of low-temperature lactic acid bacteria from seawater fish intestine and its application in preservation of Penaeus vannamei[J]. Food Science and Technology,2019,44(7):37−42. doi: 10.13684/j.cnki.spkj.2019.07.006
[52]	陶文斌. 低钠盐轻腌对养殖大黄鱼品质及风味影响研究[D]. 上海: 上海海洋大学, 2019 TAO Wenbing. Effects of low-sodium salt and light salt on the quality and flavor of cultured large yellow croaker[D]. Shanghai: Shanghai Ocean University, 2019.
[53]	吴迪迪, 李勇勇, 史咏梅, 等. 冰藏联用ClO₂保鲜对大黄鱼肌肉优势腐败菌及品质的影响[J]. 食品工业科技,2019,40(13):236−241. [WU Didi, LI Yongyong, SHI Yongmei, et al. Effects of ClO₂ preservation combined with ice storage on dominant spoilage bacteria and quality of muscle of large yellow croaker[J]. Science and Technology of Food Industry,2019,40(13):236−241.
[54]	JIAO L, TU C H, MAO J L, et al. Impact of theaflavin soaking pretreatment on oxidative stabilities and physicochemical properties of semi-dried large yellow croaker (Pseudosciaena crocea) fillets during storage[J]. Food Packaging and Shelf Life,2022,32:100852. doi: 10.1016/j.fpsl.2022.100852

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