Effects of <i>Semiliquidambar cathayensis</i> Root on Adjuvant Induced Arthritis in Rats and Analysis of Its n-Butanol Extract by UPLC-Q-TOF-MS

YIN Li; WEI Xiaomin; LU Chengshu; LU Haixiao; WANG Huakun

doi:10.13386/j.issn1002-0306.2022040259

Volume 43 Issue 22

Nov. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(22): 396-405. > DOI: 10.13386/j.issn1002-0306.2022040259

YIN Li, WEI Xiaomin, LU Chengshu, et al. Effects of Semiliquidambar cathayensis Root on Adjuvant Induced Arthritis in Rats and Analysis of Its n-Butanol Extract by UPLC-Q-TOF-MS[J]. Science and Technology of Food Industry, 2022, 43(22): 396−405. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022040259.

Citation:

PDF (19480 KB)

Effects of Semiliquidambar cathayensis Root on Adjuvant Induced Arthritis in Rats and Analysis of Its n-Butanol Extract by UPLC-Q-TOF-MS

YIN Li^{1, 2,},
WEI Xiaomin¹,
LU Chengshu¹,
LU Haixiao^{1, 2},
WANG Huakun^1, ,

1.
Yulin Normal University, Yulin 537000, China
2.
Bioengineering & Technology Center for Native Medicinal Resources Development, Yulin Normal University, Yulin 537000, China

More Information

Received Date: April 21, 2022
Available Online: September 07, 2022

Graphical Abstract

Abstract

Abstract

Objective: The effect of the Semiliquidambar cathayensis root on adjuvant-induced arthritis in rats was investigated, and the active extract and the chemical components in the root were determined. Methods: Rheumatoid arthritis model rats were divided randomly into five groups. Changes in the degree of swelling of the left hind feet and arthritis indexes were recorded. The thymus and spleen indexes of the rats were calculated after treatment. Changes in the COX-2 and 5-LOX levels in peripheral blood monocytes (PBMC) and serum IL-6, IL-1β, TNF-α, and IL-10 levels were measured. The pathomorphological changes to the rat ankle joint were observed by hematoxylin-eosin staining(HE staining). The effects of four different solvent fractions of an alcohol extract of the Semiliquidambar cathayensis root on changes to the left hind foot swelling volume and arthritis index in rats were investigated. The effective parts were analyzed qualitatively by UPLC-Q-TOF-MS. Results: The degree of foot swelling, arthritis indexes, and spleen indexes were reduced significantly in the positive drug group (1.6 g/kg) when compared with the model group. The COX-2 and 5-LOX levels in PBMC and IL-6, IL-1β, and TNF-α decreased significantly. The IL-10 serum level increased significantly. The infiltration of inflammatory cells in the joint tissue decreased, and morphology improved. The swelling volume of lateral feet and arthritis index in rats were reduced significantly in the n-butanol fraction from total alcohol extracts of the Semiliquidambar cathayensis root. One-hundred and four chemical components were identified from the n-butanol fraction, including organic acids, sugars and glycosides, flavonoids, quinones, and coumarins. Conclusion: The Semiliquidambar cathayensis root had an apparent protective effect on adjuvant arthritis-immunized rats. This root would have broad prospects in developing antiarthritic drugs and functional food.
- Semiliquidambar cathayensis root,
- rheumatoid arthritis,
- cell cytokines,
- inflammation factors,
- UPLC-Q-TOF-MS

FullText(HTML)

References (30)

References

[1]	ZULFIQAR A A, NIAZI R, PENNAFORTE J L, et al. Late-onset rheumatoid arthritis: clinical, biological, and therapeutic features about a retrospective study[J]. Geriatrie et Psychologie Neuropsychiatrie de Vieillissement,2019,17(1):51−62.
[2]	PANDEY P K, MAHESHWARI R, RAVAL N, et al. Nanogold-core multifunctional dendrimer for pulsatile chemo-, photothermal- and photodynamic-therapy of rheumatoid arthritis[J]. Journal of Colloid & Interface Science,2019,15(544):61−77.
[3]	KRZYSZTOF K, MAłGORZATA W, TACJANA B, et al. Pharmacoeconomic evaluation of costs of rheumatoid arthritis therapy with selected biological treatment[J]. Reumatologia,2018,56(6):340−345. doi: 10.5114/reum.2018.80710
[4]	张晓燕. 甘草制祖师麻炮制工艺及其抗类风湿性关节炎作用机制研究[D]. 晋中: 山西中医药大学, 2021. ZHANG X Y. Research on the processing crafts of daphne giraldii nitsche with liqueorice and its anti-rheumatoid arthritis’mechanism[D]. Jinzhong: Shanxi University of Chinese Medicine, 2021.
[5]	陈世杰, 劳文艳, 周艳丽, 等. 骨关节炎动物模型建立及在功能食品研究中的应用[J]. 中国组织工程研究,2017,21(28):4536−4542. [CHEN S J, LAO W Y, ZHOU Y L, et al. Establishment of an animal model of osteoarthritis and its application in functional food research[J]. Chinese Journal of Tissue Engineering Research,2017,21(28):4536−4542. doi: 10.3969/j.issn.2095-4344.2017.28.018
[6]	王华坤, 肖方静, 宾万娟, 等. 半枫荷调控RA模型的非靶向代谢组学研究[J]. 广西植物: 1−25 [2022-06-09]. http://kns.cnki.net/kcms/detail/45.1134.Q.20220317.0932.002.html WANG H K, XIAO F J, BIN W J, et al. Untargeted metabonomics study of semiliquidambar cathayensis in treatment of rheumatoid arthritis [J]. Guihaia: 1−25 [2022-06-09]. http://kns. cnki.net/kcms/detail/45.1134.Q.20220317.0932.002.html.
[7]	徐润, 刘少轩, 蔡卫东, 等. 半枫荷化学成分及药理活性研究进展[J]. 绿色科技,2017(1):3−4,8. [XU R, LIU S X, CAI W D, et al. Advance in chemical constituents and pharmachogical activity of semiliquidambar cathayensis[J]. Journal of Green Science and Technology,2017(1):3−4,8. doi: 10.16663/j.cnki.lskj.2017.01.003
[8]	谢石杨, 姚凯霖, 吴先基, 等. 半枫荷药学研究概况[J]. 福建林业科技,2018,45(4):122−127. [XIE S Y, YAO K L, WU X J, et al. Overview of pharmacological research on Semiliquidambar cathayensis H. T. Chang[J]. Journal of Fujian Forestry Science and Technology,2018,45(4):122−127. doi: 10.13428/j.cnki.fjlk.2018.04.024
[9]	叶兴状, 文国卫, 张明珠, 等. 珍稀濒危植物半枫荷的遗传多样性及遗传结构[J]. 植物科学学报,2021,39(4):415−423. [YE X Z, WEN G W, ZHANG M Z, et al. Genetic diversity and genetic structure of a rare and endangered speciesSemiliquidambar cathayensis Hung T. Chang[J]. Journal of Plant Science,2021,39(4):415−423. doi: 10.11913/PSJ.2095-0837.2021.40415
[10]	黄丽华, 陈秋婷, 肖雨沙, 等. 半枫荷ISSR-PCR体系优化及引物筛选[J]. 分子植物育种,2021,19(20):6782−6789. [HUANG L H, CHEN Q T, XIAO Y S, et al. Optimization and primers screening of ISSR-PCR reaction system for Semiliquidambar cathayensis Chang[J]. Molecular Plant Breeding,2021,19(20):6782−6789. doi: 10.13271/j.mpb.019.006782
[11]	卢海啸, 吕思颖, 姚水莲, 等. 金缕半枫荷甲醇部位化学成分研究[J]. 中药材,2022(3):606−610. [LU H X, LV S Y, YAO S L, et al. Chemical constituents of methanol extracts from roots of Semiliquidambar cathayensis[J]. Journal of Chinese Medicinal Materials,2022(3):606−610.
[12]	田晓明, 颜立红, 蒋利媛, 等. 基于UHPLC-QTOF/MS代谢组学技术比较分析半枫荷不同组织化学成分[J]. 植物生理学报,2021,57(6):1311−1318. [TIAO X M, YAN L H, JIANG L Y, et al. Chemical comparison of different tissues of semiliquidambar cathayensis chang by UHPLC-QTOF/MS–based metabonomics[J]. Journal of Plant Physiology,2021,57(6):1311−1318. doi: 10.13592/j.cnki.ppj.2020.0558
[13]	田晓明, 颜立红, 蒋利媛, 等. 基于UPLC/Q-TOF-MS代谢组学技术的半枫荷根化学成分分析[J]. 中国农学通报,2018,34(12):76−83. [TIAO X M, YAN L H, JIANG L Y, et al. Chemical constituents of Semiliquidambar cathayensis Chang root based on UPLC/Q-TOF/MS metabonomics technology[J]. Chinese Agricultural Science Bullet,2018,34(12):76−83. doi: 10.11924/j.issn.1000-6850.casb17120056
[14]	蒋杨, 丘晓花, 陈博, 等. 半枫荷水、乙醇提取物对小鼠的镇痛抗炎作用[J]. 广东医科大学学报,2021,39(6):672−675. [JIANG Y, QIU X H, CHEN B, et al. Analgesic and anti-inflammatory role of water and ethanol extracts from cathayensis in mice[J]. Journal of Guangdong Medical University,2021,39(6):672−675. doi: 10.3969/j.issn.1005-4057.2021.06.002
[15]	田晓明, 颜立红, 蒋利媛, 等. 半枫荷抗炎镇痛活性部位筛选及其成分分析[J]. 陕西中医药大学学报,2021,44(5):63−68. [TIAN X M, YAN L H, JIANG L Y, et al. Screening and component analysis of anti-inflammatory and analgesic active parts of banfenghe[J]. Journal of Shaanxi University of Chinese Medicine,2021,44(5):63−68.
[16]	梁伟红, 卢海啸, 曾孟. 半枫荷提取物对血瘀模型大鼠的影响[J]. 中药材,2015,38(2):366−369. [LIANG W H, LU H X, ZENG M. Effect of Semiliquidambar cathayensis Chang extract on blood stasis model rats[J]. Journal of Chinese Medicinal Materials,2015,38(2):366−369. doi: 10.13863/j.issn1001-4454.2015.02.039
[17]	卢海啸, 吴卓玲, 梁伟江, 等. 半枫荷根的化学成分研究[J]. 中药材,2015,38(12):2543−2546. [LU H X, WU Z L, LIANG W J, et al. Chemical constituents from Semiliquidambar cathayensis roots[J]. Journal of Chinese Medicinal Materials,2015,38(12):2543−2546. doi: 10.13863/j.issn1001-4454.2015.12.022
[18]	李爱民, 李晓娟, 李瑞生. 类风湿性关节炎大小鼠动物模型的研究进展[J]. 中国比较医学杂志,2021,31(1):151−156. [LI A M, LI X J, LI R S. Research progress of small animal models of rheumatoid arthritis[J]. Chinese Journal of Comparative Medicine,2021,31(1):151−156.
[19]	林兵. 豆豉姜抗类风湿性关节炎物质基础及作用机制研究[D]. 上海: 第二军医大学, 2014. LIN B. Anti-rheumatoid arthritis constituents and action mechanism of Litsea cubeba (Lour.) Pers root[D]. Shanghai: Naval Medical University, 2014.
[20]	LIU X G, ZHANG Y, JU W F, et al. MiR-21 relieves rheumatoid arthritis in rats via targeting Wnt signaling pathway[J]. European Review for Medical and Pharmacological Sciences,2019,23(3):96−103.
[21]	WANG G Y, XIE X X, YUAN L L, et al. Resveratrol ameliorates rheumatoid arthritis via activation of SIRT1-Nrf2 signaling pathway[J]. Biofactors,2020,46(3):441−453. doi: 10.1002/biof.1599
[22]	LIU K, ZHANG Y G, LIU L, et al. miR-125 regulates PI3K/Akt/mTOR signaling pathway in rheumatoid arthritis rats via PARP2[J]. Bioscience Reports,2019,39(1):BSR20180890. doi: 10.1042/BSR20180890
[23]	方慧, 熊罗节, 孙妮娜, 等. 不同灸法对免疫抑制兔脾脏、胸腺影响的组织学研究[J]. 世界科学技术-中医药现代化,2021,23(7):2523−2530. [FANG H, XIONG L J, SUN N N, et al. Effects of different moxibustion methods on spleen and thymus in immunosuppressive rabbits: A morphology study[J]. Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology,2021,23(7):2523−2530.
[24]	WANG B Q, HUANG X X, LIN J Y. Serum COX-2 and FOXO3a in patients with rheumatoid arthritis and correlation with disease activity[J]. Experiment and Therapeutic Medicine,2020,20(2):910−916. doi: 10.3892/etm.2020.8779
[25]	ZHANG X Y, DONG Y F, DONG H Y, et al. Investigation of the effect of phlomisoside F on complete Freund's adjuvant-induced arthritis[J]. Experiment and Therapeutic Medicine,2017,13(2):710−716. doi: 10.3892/etm.2016.3995
[26]	HUANG C C, CHIOU C H, LIU S C, et al. Melatonin attenuates TNF-α and IL-1β expression in synovial fibroblasts and diminishes cartilage degradation: implications for the treatment of rheumatoid arthritis[J]. Journal of Pineal Research,2019,66(3):e12560. doi: 10.1111/jpi.12560
[27]	张引红, 李美宁, 王春芳, 等. 补骨脂素对类风湿性关节炎小鼠模型的免疫调节作用[J]. 中国实验动物学报,2017,25(2):207−210. [ZHANG Y H, LI M N, WANG C F, et al. Immunoregulatory effect of psoralen on collagen-induced arthritis in mice[J]. Acta Laboratorium Animals Scientia Sinica,2017,25(2):207−210. doi: 10.3969/j.issn.1005-4847.2017.02.017
[28]	刘春红, 林丽, 肖茹雪, 等. 植物乳杆菌HG20对大鼠类风湿关节炎的改善作用[J]. 现代食品科技,2021,37(3):1−6,52. [LIU C H, LIN L, XIAO R X, et al. Improvement of rheumatoid arthritis withLactobacillus plantarum HG20 in rats[J]. Modern Food Science and Technology,2021,37(3):1−6,52. doi: 10.13982/j.mfst.1673-9078.2021.3.0711
[29]	AN Q Y, YAN W K, ZHAO Y, et al. Enhanced neutrophil autophagy and increased concentrations of IL-6, IL-8, IL-10 and MCP-1 in rheumatoid arthritis[J]. International Immunopharmacology,2018,65:119−128. doi: 10.1016/j.intimp.2018.09.011
[30]	王健, 王萍. 毛酸浆果提取物对RAW264.7细胞免疫调节作用[J]. 现代食品科技,2022,38(9):1−11. [WANG J, WANG P. Immunomodulatory effect of Physalis pubescens L. fruits extract in RAW264.7 cells[J]. Modern Food Science and Technology,2022,38(9):1−11. doi: 10.13982/j.mfst.1673-9078.2022.9.1106

Supplements (0)

Cited By

Cited by

Periodical cited type(9)

1.	吴军林，徐梅珍，蒋桂丽，杨华. 食品微生物学课程教学创新性探索及思考. 食品工业. 2025(02): 142-146 .
2.	李向梅，朱月，柳春红，徐振林，雷红涛，钟青萍. 课程思政视域下“食品卫生学”案例教学改革与育人效果评价. 食品工业. 2025(02): 237-242 .
3.	王丽娜，罗志. 土壤环境监测课程思政元素的挖掘与教学实施探索. 现代商贸工业. 2024(12): 261-263 .
4.	隋晓楠，张妍，黄国，付昳丹，梁香玉，赵尚清，王思琦，杨爱峥，霍俊伟，江连洲. 省一流专业引领下“粮油加工副产物综合利用”课程创新创业元素的设计与实践. 食品工业科技. 2024(13): 308-314 . 本站查看
5.	关海宁，刁小琴，刘登勇，励建荣，李学鹏，仪淑敏，范金波，赵志南. “乡村振兴”背景下食品类专业型硕士“实践驱动、多维融合、创新引领”协同培养机制的探讨. 食品工业科技. 2024(14): 371-377 . 本站查看
6.	林鹏，宋姗姗，范金波，李学鹏，励建荣. 基于OBE教学理念对《食品免疫学》课程教学改革与实践. 食品工业科技. 2024(14): 361-370 . 本站查看
7.	张妍，杨景旭，隋晓楠. “园艺产品采后生理及贮运加工”课程建设与创新. 食品工业. 2024(07): 221-225 .
8.	张晶，施行健，郁舒兰. 交互设计类课程中的思政元素挖掘与融合路径探索. 设计. 2024(17): 74-77 .
9.	解文丽，郭俊南，王晖，温清苹. 课程思政的实践探索——以《海水利用技术》为例. 广东化工. 2023(19): 170-173+151 .