Nutritional Composition, Antioxidant Capacity and Protective Activity against DNA Damage of Yaowang Tea (<i>Potentilla glabra</i> var.<i> mandshurica</i>) Endemic to Qinling Mountain

YE Tong; NIE Congyi; LI Xiao; ZHANG Zhanli; ZHANG Huafeng

doi:10.13386/j.issn1002-0306.2022040002

Volume 44 Issue 3

Jan. 2023

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Science and Technology of Food Industry > 2023 > 44(3): 398-405. > DOI: 10.13386/j.issn1002-0306.2022040002

YE Tong, NIE Congyi, LI Xiao, et al. Nutritional Composition, Antioxidant Capacity and Protective Activity against DNA Damage of Yaowang Tea (Potentilla glabra var. mandshurica) Endemic to Qinling Mountain[J]. Science and Technology of Food Industry, 2023, 44(3): 398−405. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022040002.

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Nutritional Composition, Antioxidant Capacity and Protective Activity against DNA Damage of Yaowang Tea (Potentilla glabra var. mandshurica) Endemic to Qinling Mountain

YE Tong^{1, 2,},
NIE Congyi^{1, 3,},
LI Xiao¹,
ZHANG Zhanli⁴,
ZHANG Huafeng^1, ,

1.
Science and Technology Innovation Experiment Demonstration Station of Se-enriched Foods in Hanyin County of Shaanxi Province, International Joint Research Center of Shaanxi Province for Food and Health Sciences, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710119, China
2.
College of Food and Wine Science, Ningxia University, Yinchuan 750021, China
3.
College of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China
4.
Shaanxi Province Plant Protection Station, Xi’an 710003, China

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Received Date: April 01, 2022
Available Online: November 28, 2022

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Abstract

Objective: In order to provide evidence to scientific exploitation of Yaowang tea endemic to Qinling mountain, nutritional value, health benefits and their material basis of Yaowang tea were investigated. Methods: Chemical composition as well as nutrients and bioactive constituents profile of Yaowang tea was analyzed by high performance liquid chromatography and plasma emission spectroscopy, etc. Antioxidant activity was determined by free radical scavenging experiment, and protective effect on DNA damage was studied by agarose gel eletrophoresis. Results: Quercitrin, quercetin and gallic acid were determined in Yaowang tea, and their contents were 0.17%±0.00%, 0.29%±0.01% and 0.28%±0.01%, respectively. Content of essential amino acids was 65.65±1.97 mg/g. Contents of manganese, selenium and magnesium were 1.19±0.04, 0.43±0.02 and 2.80±0.05 mg/kg, respectively, while heavy metals (e.g., arsenic, cadmium, mercury, lead) did not exceed the national permissible limit. Yaowang tea extract exhibited the ability to scavenge DPPH and ABTS radicals with EC₅₀ values of 0.0704 and 0.3810 mg/mL, respectively. Protective activity of Yaowang tea extract against DNA damage was in dose-dependent manner. Protective effect of 1×10⁻² mg/mL Yaowang tea extract against DNA damage was higher than that of ascorbic acid. Conclusion: Yaowang tea contains several bioactive constituents, and it is safe in respect to heavy metals. Yaowang tea possesses in vitro antioxidant activity and protective effect against DNA damage.
- Yaowang tea,
- nutritional value,
- heavy metal,
- antioxidant activity,
- DNA damage

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References

[1]	崔友文, 王作宾, 付坤俊, 等. 秦岭植物志(第一卷)[M]. 北京: 科学出版社, 北京, 1964: 547-549 CUI Y W, WANG Z B, FU K J, et al. Flora of Qinling mountain (Volume I)[M]. Beijing: Science Press, 1964: 547-549.
[2]	张华峰. 药食同源相关术语问题与对策[J]. 中国科技术语,2019,21(4):65−71. [ZHANG H F. Terms about homology between drug and food: Problems and counter measures[J]. China Terminology,2019,21(4):65−71. doi: 10.3969/j.issn.1673-8578.2019.04.012
[3]	白吉庆, 王小平, 王媚, 等. 药王茶总黄酮含量测定分析[J]. 陕西农业科学,2012,58(2):18−19. [BAI J Q, WANG X P, WANG M, et al. Determination and analysis of total flavonoids in Yaowang tea[J]. Shaanxi Agricultural Science,2012,58(2):18−19. doi: 10.3969/j.issn.0488-5368.2012.02.007
[4]	郑麟枫, 张烊烊, 刘婷, 等. 响应面法优化药王茶总黄酮提取工艺[J]. 陕西中医药大学学报,2019,42(3):51−56. [ZHENG L F, ZHANG Y Y, LIU T, et al. Optimization of extraction process of total flavonoids from Yaowang tea by response surface analysis[J]. Journal of Shaanxi University of Chinese Medicine,2019,42(3):51−56.
[5]	崔小敏, 万兆新, 任慧, 等. 基于UPLC-Q-Orbitrap HRMS技术的白毛银露梅化学成分研究[J]. 中药材,2020,43(8):1901−1906. [CUI X M, WAN Z X, REN H, et al. Study on chemical constituents of Potentilla glabra var. mandshurica Maxim based on UPLC-Q-Orbitrap HRMS technology[J]. Traditional Chinese Medicine,2020,43(8):1901−1906.
[6]	JACKSON S P, BARTEK J. The DNA-damage response in human biology and disease[J]. Nature,2009,461(7267):1071−1078. doi: 10.1038/nature08467
[7]	TANG Z Z, LIN W J, YANG J, et al. Ultrasound-assisted extraction of Cordyceps cicadae polyphenols: Optimization, LC-MS characterization, antioxidant and DNA damage protection activity evaluation[J]. Arabian Journal of Chemistry,2022,15(8):1−17.
[8]	DAS N, PARVIN M S, HASAN M, et al. A flavone from the ethyl acetate extract of Leea rubra leaves with DNA damage protection and antineoplastic activity[J]. Biochemistry and Biophysics Reports,2022,30:1−7.
[9]	YANG J, ZHANG H F, CAO X Y, et al. Enzymatic water extraction of polysaccharides from Epimedium brevicornu and their antioxidant activity and protective effect against DNA damage[J]. Journal of Food Biochemistry,2017,41(1):1−5.
[10]	国家食品药品监督管理总局, 国家卫生和计划生育委员会. GB 5009.5—2016 食品安全国家标准食品中蛋白质的测定[S]. 北京: 中国标准出版社, 2016: 3−5 China Food and Drug Administration, National Health and Family Planning Commission of the People’s Republic of China. GB 5009.5—2016 National food safety standard-Determination of protein in foods[S]. Beijing: Standards Press of China, 2016: 3−5.
[11]	罗钦, 李冬梅, 钟茂生, 等. 澳洲龙纹斑雌、雄亲鱼营养组成比较分析[J]. 福建农业学报,2020,35(1):51−58. [LUO Q, LI D M, ZHONG M S, et al. Nutritional compositions of female and male murray cod filets[J]. Fujian Journal of Agricultural Sciences,2020,35(1):51−58. doi: 10.19303/j.issn.1008-0384.2020.01.008
[12]	LI J H, HUANG J, LI C L, et al. Evaluation of the nutritional quality of edible tissues (muscle and hepatopancreas) of cultivated Procambarus clarkii using biofloc technology[J]. Aquaculture Reports,2021,19:100586. doi: 10.1016/j.aqrep.2021.100586
[13]	孙娟娟, 阿拉木斯, 赵金梅, 等. 6个紫花苜蓿品种氨基酸组成分析及营养价值评价[J]. 中国农业科学,2019,52(13):2359−2367. [SUN J J, A L M S, ZHAO J M, et al. Analysis of amino acid composition and six native alfalfa cultivars[J]. Scientia Agricultura Sinica,2019,52(13):2359−2367. doi: 10.3864/j.issn.0578-1752.2019.13.014
[14]	CHEN L, HUANG G L. Antioxidant activities of sulfated pumpkin polysaccharide[J]. International Journal of Biological Macromolecules,2019,126:743−746. doi: 10.1016/j.ijbiomac.2018.12.261
[15]	国家市场监督管理总局, 国家标准化管理委员会. GB/T 8313—2018 茶叶中茶多酚和儿茶素类含量的检测方法[S]. 北京: 中国标准出版社, 2018: 4−5 State Administration for Market Regulation, Standardization Administration of the People’s Republic of China. GB/T 8313—2018 Determination of total polyphenols and catechins content in tea[S]. Beijing: Standards Press of China, 2018: 4−5.
[16]	国家质量监督检验检疫总局, 国家标准化管理委员会. GB/T 20574—2006蜂胶中总黄酮含量的测定方法分光光度比色法[S]. 北京: 中国标准出版社, 2006: 1−2 State General Administration of the People’s Republic of China for Quality Supervision and Inspection and Quarantine, Standardization Administration of the People’s Republic of China. GB/T 20574—2006 Method for determination of total flavonoids in propolis-Colorimetric method[S]. Beijing: Standards Press of China, 2006: 1−2.
[17]	何晶晶, 杜江. 景天三七中槲皮苷、槲皮素及山萘酚含量的高效液相色谱法同时测定[J]. 时珍国医国药,2016,27(3):524−526. [HE J J, DU J. Simultaneous determinate the content of quercetin, quercitroside and kaempferol in Sedum aizoon L. by HPLC[J]. Lishizhen Medicine and Materia Medica Research,2016,27(3):524−526.
[18]	国家药典委员会. 中华人民共和国药典[M]. 北京: 中国医药科技出版社, 2020: 673 Chinese Pharmacopoeia Commission. Chinese pharmacopoeia[M]. Beijing: China Medical Science and Technology Press, 2020, 673.
[19]	国家标准化管理委员会, 国家质量监督检验检疫总局. GB/T 35871—2018粮油检验谷物及其制品中钙、钾、镁、钠、铁、磷、锌、铜、锰、硼、钡、钼、钴、铬、锂、锶、镍、硫、钒、硒、铷含量的测定电感耦合等离子体发射光谱法[S]. 北京: 中国标准出版社, 2018: 1−7 Standardization Administration of the People’s Republic of China, State General Administration of the People’s Republic of China for Quality Supervision and Inspection and Quarantine. GB/T 35871—2018 Inspection of grain and oils-Determination of calcium, potassium, magnesium, sodium, iron, phosphorus, zinc, copper, manganese, boron, barium, molybdenum, cobalt, chromium, lithium, strontium, nickel, sulfur, vanadium, selenium, rubidium contents in cereals and derived products-Inductively coupled plasma optical emission spectrometric method[S]. Beijing: Standards Press of China, 2018: 1−7.
[20]	国家卫生和计划生育委员会. GB 5009.11—2014食品安全国家标准食品中总砷及无机砷的测定[S]. 北京: 中国标准出版社, 2014: 1−3 National Health and Family Planning Commission of the People’s Republic of China. GB 5009.11—2014 National food safety standard-Determination of total arsenic and inorganic arsenic in food[S]. Beijing: Standards Press of China, 2014: 1−3.
[21]	国家卫生和计划生育委员会. GB 5009.15—2014食品安全国家标准食品中镉的测定[S]. 北京: 中国标准出版社, 2014: 1−2 National Health and Family Planning Commission of the People’s Republic of China. GB 5009.15—2014 National food safety standard-Determination of cadmium in food[S]. Beijing: Standards Press of China, 2014: 1−2.
[22]	SRIDHAR K, CHARLES A L. In vitro antioxidant activity of Kyoho grape extracts in DPPH· and ABTS· assays: Estimation methods for EC₅₀ using advanced statistical programs[J]. Food Chemistry,2019,275:41−49. doi: 10.1016/j.foodchem.2018.09.040
[23]	张昊, 魏跃伟, 姬小明, 等. 烟叶多糖硒化衍生物制备及其体外抗氧化活性研究[J]. 生物技术通报,2019,35(10):89−94. [ZHANG H, WEI Y W, JI X M, et al. Preparation of selenized derivatives from tobacco leaf polysaccharides and its antioxidant activity in vitro[J]. Biotechnology Bulletin,2019,35(10):89−94. doi: 10.13560/j.cnki.biotech.bull.1985.2019-0323
[24]	张瑞娟, 柯莉娜, 郑静, 等. 鲍鱼内脏多糖的提取、纯化及其抗氧化和抑菌活性[J]. 厦门大学学报(自然科学版),2018,57(1):58−64. [ZHANG R J, KE L N, ZHENG J, et al. Extraction, purification and antioxidant and antibacterial activity analysis of polysaccharides from abalone viscera[J]. Journal of Xiamen University (Natural Science),2018,57(1):58−64.
[25]	邓文亚, 徐婧婷, 郭顺堂, 等. 火麻仁蛋白与大豆蛋白的营养评价及比较[J]. 食品工业科技,2021,42(23):273−279. [DENG W Y, XU J T, GUO S T, et al. Nutritional evaluation and comparison of hemp seed protein and soybean protein[J]. Food Industry Technology,2021,42(23):273−279. doi: 10.13386/j.issn1002-0306.2021030019
[26]	LOVEGROVE A, EDWARDS C H, NONI I D, et al. Role of polysaccharides in food, digestion, and health[J]. Critical Reviews in Food Science and Nutrition,2017,57(2):237−253. doi: 10.1080/10408398.2014.939263
[27]	LUCA S V, MACOVEIA I, BUJOR A, et al. Bioactivity of dietary polyphenols: The role of metabolites[J]. Critical Reviews in Food Science and Nutrition,2020,60(4):626−659. doi: 10.1080/10408398.2018.1546669
[28]	ZHAO C Y, WANG F, LIAN Y H, et al. Biosynthesis of citrus flavonoids and their health effects[J]. Critical Reviews in Food Science and Nutrition,2020,60(4):566−583. doi: 10.1080/10408398.2018.1544885
[29]	ROMERO M, VERA B, GALISTEO M, et al. Protective vascular effects of quercitrin in acute TNBS-colitis in rats: The role of nitric oxide[J]. Food & Function,2017,8:2702−2711.
[30]	KAWABATA K, MUKAIB R, ISHISAKA A. Quercetin and related polyphenols: New insights and implications for their bioactivity and bioavailability[J]. Food & Function,2015,6(5):1399−1417.
[31]	BATIHA G E, BESHBISHY A M, IKRAM M, et al. The pharmacological activity, biochemical properties, and pharmacokinetics of the major natural polyphenolic flavonoid: Quercetin[J]. Foods,2020,9(3):374−390. doi: 10.3390/foods9030374
[32]	KAHKESHANI N, FARZAEI F, FOTOUHI M, et al. Pharmacological effects of gallic acid in health and diseases: A mechanistic review[J]. Iranian Journal of Basic Medical Sciences,2019,22:225−237.
[33]	国家卫生和计划生育委员会, 国家食品药品监督管理总局. GB 2762—2017食品安全国家标准食品中污染物限量(含第1号修改单)[S]. 北京: 中国标准出版社, 2017: 1−22 National Health and Family Planning Commission of the People’s Republic of China. GB 2762—2017 National food safety standard-Limits of contaminants in food (Including amendment No. 1)[S]. Beijing: Standards Press of China, 2017: 1−22.
[34]	BELAIDI A A, BUSH A I. Iron neurochemistry in Alzheimer’s disease and Parkinson’s disease: Targets for therapeutics[J]. Journal of Neurochemistry,2016,139(1):179−197.
[35]	邓绍林. 广西甜茶叶片的营养成分及开发利用价值研究[J]. 中国林副特产,2000,3(54):18−19. [DENG S L. Study on nutritional components and development and utilization value of Guangxi sweet tea leaves[J]. Quarterly of Forest By-Product and Speciality in China,2000,3(54):18−19. doi: 10.3969/j.issn.1001-6902.2000.03.012
[36]	LUZA E P C G, CHAGASB B S D, ALMEIDA N T D, et al. Resorbable bacterial cellulose membranes with strontium release for guided bone regeneration[J]. Materials Science & Engineering C,2020,116:1−14.
[37]	XIA X J, ZHANG X L, LIU M C, et al. Toward improved human health: Efficacy of dietary selenium on immunity at the cellular level[J]. Food & Function,2021,12(3):976−989.
[38]	RAYMAN M P. Selenium and human health[J]. Lancet,2012,379(5):1256−1268.
[39]	MOGHADDAM A, HELLER R A, SUN Q, et al. Selenium deficiency is associated with mortality risk from COVID-19[J]. Nutrients, 2020, 12(7) 2098: 1−13.
[40]	NRIAGU J. Zinc deficiency in human health[J]. Encyclopedia of Environmental Health,2011,1(1):789−800.
[41]	HAMMARSTEN J F, ALLGOOD M, SMITH W O. Effects of magnesium sulfate on renal function, electrolyte excretion and clearance of magnesium[J]. Journal of Applied Physiology,1957,10(3):476−478. doi: 10.1152/jappl.1957.10.3.476
[42]	SILVA L F D, AMBROSIO F R. A review of the action of magnesium on several processes involved in the modulation of hematopoiesis[J]. International Journal of Molecular Sciences, 2020, 21(19): 7084.
[43]	LIMMONGKON A, PANKAM J, SOMBOON T, et al. Evaluation of the DNA damage protective activity of the germinated peanut (Arachis hypogaea) in relation to antioxidant and anti-inflammatory activity[J]. LWT,2019,101(C):259−268.
[44]	ADET J, DAVIES K J A. Oxidative DNA damage & repair: An introduction[J]. Free Radical Biology and Medicine,2017,107(6):6−12.
[45]	PENA-BAUTISTA C, TIRLE T, LOPEZ-NOGUEROLES M, et al. Oxidative damage of DNA as early marker of Alzheimer’s disease[J]. International Journal of Molecular Sciences,2019,20(24):1−13.