A Review of Methods and Progress in Highly Efficient Screening of Antimicrobial Peptides from Natural Products

YANG Hongyan; SHAN Zixuan; LAI Liang; DUAN Jun; WANG Jincai; HUO Jiayin; GUO Jialiang

doi:10.13386/j.issn1002-0306.2022030395

Volume 43 Issue 21

Oct. 2022

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Science and Technology of Food Industry > 2022 > 43(21): 28-35. > DOI: 10.13386/j.issn1002-0306.2022030395

YANG Hongyan, SHAN Zixuan, LAI Liang, et al. A Review of Methods and Progress in Highly Efficient Screening of Antimicrobial Peptides from Natural Products[J]. Science and Technology of Food Industry, 2022, 43(21): 28−35. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030395.

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A Review of Methods and Progress in Highly Efficient Screening of Antimicrobial Peptides from Natural Products

1.
School of Medicine, Foshan University, Foshan 528000, China
2.
Pharmacy College, Jinan University, Guangzhou 510632, China
3.
Sinopharm Group Fengliaoxing (Foshan) Pharmaceutical Co., Ltd., Foshan 528000, China

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Received Date: March 31, 2022
Available Online: August 25, 2022

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Abstract

Abstract

Natural antimicrobial peptides (AMPs) are promising candidates for developing a generation of new antimicrobials to meet the challenge of antibiotic-resistant pathogens, and they are extensively applied in medicine, food, agriculture and other fields. However, the rapid screening of AMPs from natural products still has many difficulties and challenges, such as low efficiency and high consumption. This paper firstly introduces the action mechanism of AMPs (including membrane action mechanism and non-membrane action mechanism); then, the highly efficient screening methods of AMP are systematically reviewed, including bacterial adsorption, cell membrane chromatography, phospholipid membrane chromatography, capillary electrophoresis, colorimetry, thin layer chromatography, fluorescence screening, high-throughput sequencing, and mining databases screening. In addition, the development direction of efficient exploration of AMP is also prospected. This paper provides a scientific basis for the discovery of AMPs from complex natural product systems.
- antimicrobial peptides,
- natural products,
- high-throughput screening,
- mechanism of membrane action

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References

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