Study of <i>Lacticaseibacillus rhamnosus</i> MP108 on Alleviating Bacterial Diarrhea

ZHAO Yueming; HOU Yanmei; ZHAO Yiqing

doi:10.13386/j.issn1002-0306.2022030306

Volume 43 Issue 21

Oct. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(21): 20-27. > DOI: 10.13386/j.issn1002-0306.2022030306

ZHAO Yueming, HOU Yanmei, ZHAO Yiqing. Study of Lacticaseibacillus rhamnosus MP108 on Alleviating Bacterial Diarrhea[J]. Science and Technology of Food Industry, 2022, 43(21): 20−27. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030306.

Citation:

PDF (9134 KB)

Study of Lacticaseibacillus rhamnosus MP108 on Alleviating Bacterial Diarrhea

ZHAO Yueming^{1, 2,},
HOU Yanmei^{2, 3},
ZHAO Yiqing^2, ,

1.
Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin 150030, China
2.
Hyproca Nutrition Co., Ltd., Changsha 410011, China
3.
Hunan Provincial Key Laboratory of Food Science and Biotechnology, Changsha 410125, China

More Information

Received Date: March 24, 2022
Available Online: August 27, 2022

Graphical Abstract

Abstract

Abstract

The alleviation effect of Lacticaseibacillus rhamnosus MP108-containing products on diarrhea caused by Enterotoxic Escherichia coli was evaluated. Six-week-old male BALB/c mice were randomly divided into blank group, model group, MP108 probiotic product group, and high-dose MP108 probiotic product group, and probiotic interventions lasted for two weeks. After the experiment, the fecal water content, serum diarrhea-related proteins, inflammatory factors, pathological changes of jejunum tissue, and gut microbial alterations were evaluated. The results showed that both the MP108 product group and the high-dose MP108 product group relieved diarrhea symptoms caused by Enterotoxic Escherichia coli (ETEC) via reducing jejunum damage, decreasing the levels of serum inflammatory factors, suppressing the expressions of serum ferritin (ST) and Toll-like receptor 4 (TLR4), and increasing aquaporin 3 (AQP3) levels. Intestinal flora analysis showed that L. rhamnosus MP108 product restored the intestinal microecology and increased the proportions of short-chain fatty acid-producing bacteria including Akkermansia, Ruminococcaceae, Lachnospiraceae. Collectively, L. rhamnosus MP108 probiotic products relieve diarrhea by regulating the expression of diarrhea-related proteins and inflammatory factors and modulating gut microbiota.
- Lacticaseibacillus rhamnosus,
- Escherichia coli,
- diarrhea,
- inflammatory factors,
- gut microbiota

FullText(HTML)

References (33)

References

[1]	LANATA C F, FISCHER-WALKER C L, OLASCOAGA A C, et al. Global causes of diarrheal disease mortality in children <5 years of age: A systematic review[J]. PloS One,2013,8(9):e72788. doi: 10.1371/journal.pone.0072788
[2]	WANG H, ZHONG Z, LUO Y, et al. Heat-stable enterotoxins of enterotoxigenic Escherichia coli and their impact on host immunity[J]. Toxins,2019,11(1):24. doi: 10.3390/toxins11010024
[3]	VIRK A, MANDREKAR J, BERBARI E F, et al. A randomized, double blind, placebo-controlled trial of an oral synbiotic (AKSB) for prevention of travelers' diarrhea[J]. Journal of Travel Medicine,2013,20(2):88−94. doi: 10.1111/jtm.12008
[4]	XU H, ZHAO F, HOU Q, et al. Metagenomic analysis revealed beneficial effects of probiotics in improving the composition and function of the gut microbiota in dogs with diarrhoea[J]. Food & Function,2019,10(5):2618−2629.
[5]	YANG B, YUE Y, CHEN Y, et al. Lactobacillus plantarum CCFM1143 alleviates chronic diarrhea via inflammation regulation and gut microbiota modulation: A double-blind, randomized, placebo-controlled study[J]. Frontiers in Immunology,2021,12:746585. doi: 10.3389/fimmu.2021.746585
[6]	WANG J, JI H, WANG S, et al. Probiotic Lactobacillus plantarum promotesintestinal barrier function by strengthening the epithelium and modulating gut microbiota[J]. Frontiers in Microbiology,2018,9:1953. doi: 10.3389/fmicb.2018.01953
[7]	LIU T Y, SONG X L, AN Y P, et al. Lactobacillus rhamnosus GG colonization in early life ameliorates inflammaging of offspring by activating SIRT1/AMPK/PGC-1 alpha pathway[J]. Oxidative Medicine and Cellular Longevity,2021,21(27):3328505.
[8]	ROSELLI M, FINAMORE A, BRITTI M S, et al. Probiotic bacteria Bifidobacterium animalis MB5 and Lactobacillus rhamnosus GG protect intestinal Caco-2 cells from the inflammation-associated response induced by enterotoxigenic Escherichia coli K88[J]. The British Journal of Nutrition,2006,95(6):1177−1184. doi: 10.1079/BJN20051681
[9]	YANG B, HUANG Z, He Z, et al. Protective effect of Bifidobacterium bifidum FSDJN7O5 and Bifidobacterium breve FHNFQ23M3 on diarrhea caused by enterotoxigenic Escherichia coli[J]. Food & Function,2021,12(16):7271−7282.
[10]	靳志强, 王延祥, 李平兰, 等. 植物乳杆菌耐酸耐胆盐的体外试验及其降胆固醇作用[J]. 中国食品学报,2009,9(5):24−28. [XING Z Q, WANG Y X, LI P L, et al. Studies on the acid and bile salt resistant ability of Lactobacillus plantarum and its effect of cholesterol reducing in vitro[J]. Journal of Chinese Institute of Food Science and Technology,2009,9(5):24−28. doi: 10.3969/j.issn.1009-7848.2009.05.004
[11]	LEE H, KIM J, JEUNG H, et al. Effects of Ficuscarica paste on loperamide-induced constipation in rats[J]. Food and Chemical Toxicology,2012,50(3-4):895−902. doi: 10.1016/j.fct.2011.12.001
[12]	BOLYEN E, RIDOUT J R, DILLON M R, et al. Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2[J]. Nature Biotechnology,2019,37(8):852−857. doi: 10.1038/s41587-019-0209-9
[13]	NOVAL R M, BURTON O T, WISE P, et al. A microbiota signature associated with experimental food allergy promotes allergic sensitization and anaphylaxis[J]. Journal of Allergy and Clinical Immunology,2013,131(1):201−212. doi: 10.1016/j.jaci.2012.10.026
[14]	SEGATA N, IZARD J, WALDRON L, et al. Metagenomic biomarker discovery and explanation[J]. Genome Biology,2011,12(6):R60. doi: 10.1186/gb-2011-12-6-r60
[15]	王春荣, 刘学飞, 程永刚. 肠功能恢复汤对感染性多器官功能障碍综合征大鼠肠黏膜损伤的保护作用[J]. 郑州大学学报(医学版),2008(6):1107−1111. [[WANG C R, LIU X F, CHENG Y G, et al. Protection of intestine function recovery decoction on intestinal mucosa injury for multiple organ dysfunction syndrom rats caused by infection[J]. Joumal of Zhengzhou University (Medircal Sciences),2008(6):1107−1111. doi: 10.13705/j.issn.1671-6825.2008.06.015
[16]	赵凯科, 韩军艳, 郑立, 等. 葎草醇提物对小鼠细菌性腹泻的治疗作用[J]. 中国兽医学报,2015,35(11):1814−1818. [ZHAO K K, HAN J Y, ZHENG L, et al. The rapeutic effect on Humulus scandens alcohol extract on bacterial diarrhea of mice[J]. Chinese Journal of Veterinary Science,2015,35(11):1814−1818. doi: 10.16303/j.cnki.1005-4545.2015.11.17
[17]	YANG K M, JIANG Z Y, ZHENG C T, et al. Effect of Lactobacillus plantarum on diarrhea and intestinal barrier function of young piglets challenged with enterotoxigenic Escherichia coli K88[J]. Journal of Animal Science,2014,92(4):1496−1503. doi: 10.2527/jas.2013-6619
[18]	XINGTAO Z, DUODUO Z, WUCHENG Q, et al. Exopolysaccharides from Lactobacillus plantarum NCU116 facilitate intestinal homeostasis by modulating intestinal epithelial regeneration and microbiota[J]. Journal of Agricultural and Food Chemistry,2021,69(28):7863−7873. doi: 10.1021/acs.jafc.1c01898
[19]	IKARASHI N, KON R, SUGIYAMA K. Aquaporins in the colon as a new therapeutic target in diarrhea and constipation[J]. International Journal of Molecular Sciences,2016,17(7):1172. doi: 10.3390/ijms17071172
[20]	PERDIGÓN G, VINTIÑI E, ALVAREZ S, et al. Study of the possible mechanisms involved in the mucosal immune system activation by lactic acid bacteria[J]. Journal of Dairy Science,1999,82(6):1108−1114. doi: 10.3168/jds.S0022-0302(99)75333-6
[21]	SOOMRO S, VENKATESWARAN S, VANARSA K, et al. Predicting disease course in ulcerative colitis using stool proteins identified through an aptamer-based screen[J]. Nature Communications,2021,12(1):3989. doi: 10.1038/s41467-021-24235-0
[22]	ALVESTEGUI A, OLIVARES-MORALES M, MUÑOZ E, et al. TLR4 participates in the inflammatory response induced by the AAF/II fimbriae from Enteroaggregative Escherichia coli on intestinal epithelial cells[J]. Front Cell Infect Microbiol,2019,9:143. doi: 10.3389/fcimb.2019.00143
[23]	岳月, 杨波, 赵建新, 等. 植物乳杆菌通过肠道菌群调节缓解产肠毒素大肠杆菌导致的腹泻[C]. 第十五届益生菌与健康国际研讨会, 2020: 2. [YUE Y, YANG B, ZHAO J X, et al. Lactobacillus plantarum alleviates diarrhea caused by Enterotoxogenic Escherichia coli by regulating gut microbiota[C]. The 15th International Symposium on Probiotics and Health, 2020: 2.
[24]	张睦清, 彪雅宁, 李易水, 等. 车前子对腹泻大鼠小肠组织AQP3基因及蛋白表达的影响[J]. 新中医,2021,53(17):1−5. [[ZHANG M Q, BIAO Y N, LI Y S, et al. Effect of semen plantaginis on expression of AQP3 gene and protein in small intestine of diarrhea rats[J]. New Chinese Medicine,2021,53(17):1−5. doi: 10.13457/j.cnki.jncm.2021.17.001
[25]	丁晓洁, 孙喜灵, 于晓飞, 等. 乌梅丸对腹泻型肠易激综合征模型大鼠肠道菌群和炎症因子的影响[J]. 辽宁中医杂志,2019,46(6):1296−1299. [DING X J, SUN X L, YU X F, et al. Effect of Wumei pill on intestinal flora and inflammatory factors in rats with diarrhea-type irritable bowel syndrome[J]. Liaoning Traditional Chinese Medicine Journal,2019,46(6):1296−1299. doi: 10.13192/j.issn.1000-1719.2019.06.056
[26]	CASTRO-MEJIA J L, O'FERRALL S, KRYCHA L, et al. Restitution of gut microbiota in Ugandan children administered with probiotics (Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis BB-12) during treatment for severe acute malnutrition[J]. Gut Microbes,2020,11(4):13.
[27]	BIN P, TANG Z, LIU S, et al. Intestinal microbiota mediates enterotoxigenic Escherichia coli-induced diarrhea in piglets[J]. BMC Veterinary Research,2018,14(1):385. doi: 10.1186/s12917-018-1704-9
[28]	LIU S M, LI E Y, SUN Z Y, et al. Altered gut microbiota and short chain fatty acids in Chinese children with autism spectrum disorder[J]. Scientific Reports,2019,9(1):287. doi: 10.1038/s41598-018-36430-z
[29]	LIU C, LIANG X, WEI X, et al. Gegen Qinlian decoction treats diarrhea in piglets by modulating gut microbiota and short-chain fatty acids[J]. Frontiers in Microbiology,2019,10:825. doi: 10.3389/fmicb.2019.00825
[30]	YU Y, LU J, SUN L, et al. Akkermansia muciniphila: A potential novel mechanism of nuciferine to improve hyperlipidemia[J]. Biomedicine Pharmacotherapy,2021,133:111014. doi: 10.1016/j.biopha.2020.111014
[31]	LÓPEZ-COLOM P, CASTILLEJOS L, RODRIGUEZ-SORRENTO A, et al. Impact of in-feed sodium butyrate or sodium heptanoate protected with medium-chain fatty acids on gut health in weaned piglets challenged with Escherichia coli F4⁺[J]. Archives of Animal Nutrition,2020,74(4):271−295. doi: 10.1080/1745039X.2020.1726719
[32]	WANG Q, HE Y, LI X, et al. Lactobacillus reuteri CCFM8631 alleviates hypercholesterolaemia caused by the Paigen atherogenic diet by regulating the gut microbiota[J]. Nutrients,2022,14(6):1272. doi: 10.3390/nu14061272
[33]	MOORTHY M, WIE CC, MARIÑO E, et al. The prebiotic potential of geraniin and geraniin-enriched extract against high-fat-diet-induced metabolic syndrome in spraguedawleyrats[J]. Antioxidants (Basel),2022,11(4):632. doi: 10.3390/antiox11040632

Supplements (0)

Cited By

Cited by

Periodical cited type(4)

1.	孙怀竹，梁金玲，许天阳，于澎. 干燥对不同药用部位中草药化学成分影响的研究进展. 中华中医药学刊. 2025(02): 246-250 .
2.	王慧，杨馥源，王玉龙，邢普，闫艳，杜晨晖. 基于多指标综合分析酸枣仁最佳采收期的质量标志物研究. 中国野生植物资源. 2024(04): 1-9+21 .
3.	刘天悦，滕晓祎，田永涛，么亚妹，王琰博，王文蜀. 灯笼果叶片、宿萼和果实化学成分分析及抗氧化活性评价. 食品与发酵工业. 2024(20): 243-251 .
4.	谢玉蓉，邵大畏，贺建红. 扶正益气方对乳腺癌术后化疗患者不良反应的影响及改善生活质量的状况. 辽宁中医杂志. 2024(11): 138-141 .