Optimization of Extraction Process of Polysaccharides from<i> Olea europaea</i> L. Leaves and Its Physicochemical Properties and Antioxidant Activity

REN Yijie; ZHAO Xiaoliang; WANG Baozhong; XIANG Zijun; YANG Chaofu; WANG Haili; MA Junyi; ZHANG Weijie

doi:10.13386/j.issn1002-0306.2022030302

Volume 43 Issue 23

Nov. 2022

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Science and Technology of Food Industry > 2022 > 43(23): 245-251. > DOI: 10.13386/j.issn1002-0306.2022030302

REN Yijie, ZHAO Xiaoliang, WANG Baozhong, et al. Optimization of Extraction Process of Polysaccharides from Olea europaea L. Leaves and Its Physicochemical Properties and Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(23): 245−251. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030302.

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Optimization of Extraction Process of Polysaccharides from Olea europaea L. Leaves and Its Physicochemical Properties and Antioxidant Activity

1.
Gansu Longshen Rongfa Pharmaceutical Industry Co., Ltd., Lanzhou 730102, China
2.
Gansu Provincial Key Laboratory of Solid Dispersible Preparations for Traditional Chinese Medicine, Lanzhou 730102, China
3.
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China
4.
College of Life Sciences, Northwest Normal University, Lanzhou 730070, China

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Received Date: March 23, 2022
Available Online: October 09, 2022

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Abstract

Abstract

In order to increase the development and utilization value of polysaccharides from Olea europaea L. leaves, the extraction process of polysaccharides from Olea europaea L. leaves (OLP) was optimized by orthogonal experiment, the molecular weight was determined by high performance gel permeation chromatography combined with multi-angle laser light scattering (HPGPC-MALLS), and the monosaccharide composition of OLP was analyzed by PMP pre-column derivatization and its antioxidant activity was evaluated. The results showed that the optimal extraction conditions were material-liquid ratio of 1:27.5 g/mL, extraction temperature of 95 ℃ and extraction time of 3.5 h. Under these conditions, the yield of OLP was 2.75%. The average weight molecular weight (Mw) of OLP was 25.36 kDa, the average number molecular weight (Mn) was 19.32 kDa, and the polydispersity was 1.313. OLP was mainly composed of glucose (Glc), galactose (Gal) and galactose amino (GalN), but also contains rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man) and glucosamine (GlcN), and the relative molar ratio of monosaccharide was 56.2:15.9:10.3:8.3:5.9:2.6:0.5:0.3. The results showed that OLP had good antioxidant activity, with IC₅₀ of 0.422, 0.302 and 0.268 mg/mL for hydroxyl free radical, superoxide anion free radical and DPPH free radical, respectively. The optimum technology of OLP is simple, the yield is high, and the antioxidant activity is good, which provides an essential reference for the further research, development and utilization of OLP.
- Olea europaea L. leaves,
- polysaccharide,
- orthogonal test,
- physicochemical properties,
- antioxidant activity

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