Extraction Process Optimization，Purification and Characterization Analysis of Glycoprotein from the Mucus of Chinese Giant Salamander

TANG Jiacheng; BAO Jianqiang; CHEN Yanjie; SHAN Qianyi; HUANG Kecheng; GONG Xuan

doi:10.13386/j.issn1002-0306.2022030284

Volume 43 Issue 21

Oct. 2022

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Science and Technology of Food Industry > 2022 > 43(21): 252-261. > DOI: 10.13386/j.issn1002-0306.2022030284

TANG Jiacheng, BAO Jianqiang, CHEN Yanjie, et al. Extraction Process Optimization，Purification and Characterization Analysis of Glycoprotein from the Mucus of Chinese Giant Salamander[J]. Science and Technology of Food Industry, 2022, 43(21): 252−261. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030284.

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Extraction Process Optimization，Purification and Characterization Analysis of Glycoprotein from the Mucus of Chinese Giant Salamander

1.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
2.
Shanghai Engineering Research Center of Aquatic Product Processing and Preservation, Shanghai 201306, China
3.
Ministry of Agriculture Laboratory for Quality, Safety and Risk Assessment of Storage and Preservation of Aquatic Product (Shanghai), Shanghai 201306, China
4.
Shanghai Jing'an District Market Supervision Administration, Shanghai 200072, China

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Received Date: March 22, 2022
Available Online: August 28, 2022

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Abstract

Abstract

The extraction process of crude glycoprotein from Chinese giant salamander (MGP) mucus cultured in Lishui was optimized. The crude glycoprotein was further purified, of which characterization was analysed. Based on single factor experiment, the extraction process was optimized by response surface methodology with solid-liquid ratio, temperature, extraction concentration, extraction time as factors, and glycoprotein yield as index. The results showed that the optimal extraction conditions were solid-liquid ratio of 1:30.5 g/mL, extraction temperature of 51 ℃, extraction concentration of 0.32 mol/L, extraction time of 5.2 h, the highest yield of MGP was 5.22%±0.71%, which was basically consistent with the expected results. The electrophoresis pure of purified glycoprotein was determined. The lyophilized MGP was white, loose powder, of which molecular weight was about 29 kDa. The contents of essential amino acids and medicinal amino acids in MGP were 37.21 g/100 g and 33.39 g/100 g, respectively. MGP had a typical glycoprotein structure of UV full wavelength absorption image, containing o-glycopeptide bond, pyranose ring structure and β-glycosidic bond. The secondary structure of MGP was mainly composed of 87% β-folded, which provided a theoretical basis for further research on the biological activity of glycoprotein from the mucus of Chinese giant salamander.
- Chinese giant salamander mucus glycoprotein (MGP),
- extraction process,
- yield,
- amino acid composition,
- characterization analysis

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