SONG Yu, ZHENG Jian, HUANG Feng, et al. Effects of Different Salting Methods on the Quality Traits, Histomorphology and Protein Structure of Cooked Pork Steaks[J]. Science and Technology of Food Industry, 2022, 43(23): 103−111. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030282.
Citation: SONG Yu, ZHENG Jian, HUANG Feng, et al. Effects of Different Salting Methods on the Quality Traits, Histomorphology and Protein Structure of Cooked Pork Steaks[J]. Science and Technology of Food Industry, 2022, 43(23): 103−111. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030282.

Effects of Different Salting Methods on the Quality Traits, Histomorphology and Protein Structure of Cooked Pork Steaks

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  • Received Date: March 22, 2022
  • Available Online: September 28, 2022
  • The aim of this study was to investigate the effects of different salting methods (wet salting, dry salting, ultrasonic assisted wet salting) on quality traits (color, texture, water retention capacity), histomorphology and protein structure of cooked pork steaks. Longissimus thoracis et lumborum (LTL) muscles of pork were selected as experimental material, and the meat samples were salted by different methods and then cooked. Meat quality, water distribution, histomorphology, protein surface hydrophobicity and protein secondary structure of cooked meat were determined. The results showed that different salting methods had significant influences on the quality traits of cooked pork steaks. In comparison with the other two salting methods, the sample salted by ultrasonic had the lowest cooking loss, the highest redness value, and the highest viscosity and tenderness. The results of water distribution showed that the longitudinal (T1) and transverse (T2) relaxation intensity were the highest in samples of ultrasonic assisted salting. Meanwhile, the transverse relaxation time was the longest in cooked samples of ultrasonic-assisted salting, the proportion of immobilized water was the highest, and the proportion of free water was the lowest. Ultrasonic assisted salting increased muscle fiber swelling, and individual myofibers in the salted tissue could be discerned, perhaps due to the degradation of connective tissue during salting. Additionally, the cooked meat samples of ultrasonic assisted salting had the strongest protein hydrophobicity ability. Protein secondary structure analyses showed that the meat samples in ultrasonic assisted salting group exhibited the lowest content of α-helix and β-turn, and the highest content of β-sheet and random coil, indicating the highest degree of protein unfolding. Moreover, protein structural changes in cooked meat were significantly correlated with meat quality traits.
  • [1]
    孟彬, 王小乔, 张静. 中国肉制品发展趋势[J]. 肉类工业,2011(8):6−8. [MENG Bin, WANG Xiaoqiao, ZHANG Jing. Development trend of meat products in China[J]. Meat Industry,2011(8):6−8. doi: 10.3969/j.issn.1008-5467.2011.08.003
    [2]
    郭昕, 黄峰, 张春江, 等. 静态变压腌制技术对猪肉品质的影响[J]. 中国农业科学,2015,48(11):2229−2240. [GUO Xin, HUANG Feng, ZHANG Chunjiang, et al. Effects of pressure varied static brining on pork quality[J]. Scientia Agricultura Sinica,2015,48(11):2229−2240. doi: 10.3864/j.issn.0578-1752.2015.11.014
    [3]
    王静帆, 黄峰, 沈青山, 等. 低温长时蒸煮对猪肉品质的影响[J]. 中国农业科学,2021,54(3):643−652. [WANG Jingfan, HUANG Feng, ZHANG Chunhui, et al. Effects of low temperature and long time cooking on pork quality[J]. Agricultural Sciences in China,2021,54(3):643−652. doi: 10.3864/j.issn.0578-1752.2021.03.017
    [4]
    康大成. 超声波辅助腌制对牛肉品质的影响及其机理研究[D]. 南京: 南京农业大学, 2017.

    KANG Dacheng. Effects and mechanism of ultrasound-assisted curing on the quality of beef[D]. Nanjing: Nanjing Agricultural University, 2017.
    [5]
    CHEN X, LUO A, WANG Y, et al. Duck breast muscle proteins, free fatty acids and volatile compounds ad affected by curing methods[J]. Food Chemistry,2021,338:128138. doi: 10.1016/j.foodchem.2020.128138
    [6]
    黄瀚, 不同腌制方式对兔肉及其产品加工过程品质的影响[D]. 重庆: 西南大学, 2016.

    HUANG Han. Effects of different curing methods on the quality of rabbit meat and its products during processing[D]. Chongqing: Southwest University, 2016.
    [7]
    郭雅, 卞欢, 江芸等. 不同腌制方式对风干鳊鱼理化指标的影响[J]. 食品工业科技,2020,36(4):228−234. [ZHANG Jingjing, LIU Guiqin, WEI Zixiang, et al. Dynamic change of physicochemical properties of dry-cured donkey ham during processing[J]. Modern Food Technology,2020,36(4):228−234. doi: 10.13386/j.issn1002-0306.2016.14.046
    [8]
    QIN N, ZHANG L T, ZHANG J B, et al. Influence of lightly salting and sugaring on the quality and water distribution of grass carp (Ctenopharyngodon idellus) during super-chilled storage[J]. Journal of Food Engineering,2017,215:104−112. doi: 10.1016/j.jfoodeng.2017.07.011
    [9]
    WANG X J, MUHOZA B, WANG X W, et al. Comparison between microwave and traditional water bath cooking on salting perception, water distribution and microstructure of grass crap meat[J]. Food Research International,2019,125:108521. doi: 10.1016/j.foodres.2019.108521
    [10]
    CAO C N, FENG Y Y, KONG B H, et al. Texture and gel properties of frankfurters as influenced by various k-carrageenan incorporation methods[J]. Meat Science,2021,176:108483. doi: 10.1016/j.meatsci.2021.108483
    [11]
    WANG S Q, LIN R, CHENG S S. Water dynamics changes and protein denaturation in surf clam evaluated by two-dimensional LF-NMR T1-T2 relaxation technique during heating process[J]. Food Chemistry,2020,320:126622. doi: 10.1016/j.foodchem.2020.126622
    [12]
    王策. 含氧气调包装对冷却肉持水性的影响机制[D]. 北京: 中国农业科学院, 2018.

    WANG Ce. The effect of different oxygen concentrations modified atmosphere packaging on water holding capacity of chilled meat[D]. Beijing: Chinese Academy of Agricultural Sciences, 2018.
    [13]
    JIANG Q Q, JIA R, NAKAZAWA N, et al. Changes in protein properties and tissue histology of tuna meat as affected by salting and subsequent freezing[J]. Food Chemistry,2019,271:550−560. doi: 10.1016/j.foodchem.2018.07.219
    [14]
    MITRA B, RINNANA, RUIZ-CARRASCAL J, et al. Tracking hydrophobicity state, aggregation behavior and structural modifications of pork proteins under the influence of associated heat treatments[J]. Food Research International,2017,101:266−273. doi: 10.1016/j.foodres.2017.09.027
    [15]
    戴研. 欧姆加热对猪肉蛋白质降解、氧化以及凝胶特性的影响[D]. 北京: 中国农业大学, 2014.

    DAI Yan. Effects of ohmic heating on protein degradation, oxidation and gel properties of pork [D]. Beijing: China Agricultural University, 2014.
    [16]
    HUGHES J, CLARKE F, LI Y, et al. Differences in light scattering between pale and dark beef Longissimus thoracis muscles are primarily caused by differences in the myofilament lattice, myofibril and muscle fiber transverse spacings[J]. Meat Science,2019,149:96−106. doi: 10.1016/j.meatsci.2018.11.006
    [17]
    BECKER A, BOULAABE A, PINGEN S, et al. Low temperature cooking of pork meat-physicochemical and sensory aspects[J]. Meat Science,2016,118:82−88. doi: 10.1016/j.meatsci.2016.03.026
    [18]
    刘成花, 李顺, 张雅伟, 等. 低钠干腌肉加工过程中肌肉结缔组织特性[J]. 食品科学,2018,39(1):91−98. [LIU Chenghua, LI Shun, ZHANG Yawei, et al. Characteristics of muscle connective tissue during low-sodium dry-cured meat processing[J]. Food Science,2018,39(1):91−98.
    [19]
    潘琼. 超声辅助干腌对低钠盐培根品质的影响及机理研究[D]. 合肥: 合肥工业大学, 2020.

    PAN Qiong. Effect and mechanism of ultrasound-assisted dry curing on the quality of low sodium bacon[D]. Hefei: Hefei University of Technology, 2020.
    [20]
    SIRO I, VENN C, BALLA C, et al. Application of an ultrasonic assisted curing technique for improving the diffusion of sodium chloride in porcine meat[J]. Journal of Food Engineering,2009,91:353−362. doi: 10.1016/j.jfoodeng.2008.09.015
    [21]
    BERTRAM H C, PURSLOW P P, ANDERSEN H J. Relationship between meat structure, water mobility, and distribution: A low-field nuclear magnetic resonance study[J]. Journal of Agricultural and Food Chemistry,2002,50:824−829. doi: 10.1021/jf010738f
    [22]
    SHAO J H, DENG Y M, JIA N. Low-field NMR determination of water distribution in meat batters with NaCl and polyphosphate addition[J]. Food Chemistry,2016,200:308−314. doi: 10.1016/j.foodchem.2016.01.013
    [23]
    MCDONNELLC K, ALLEN P, MORIN C, et al. The effect of ultrasonic salting on protein and water-protein interactions in meat[J]. Food Chemistry,2014,147:245−251. doi: 10.1016/j.foodchem.2013.09.125
    [24]
    SUN Y, MA L, FU Y. The improvement of gel and physicochemical properties of porcine myosin under low salt concentrations by pulsed ultrasound treatment and its mechanism[J]. Food Research International,2021,141:110056. doi: 10.1016/j.foodres.2020.110056
    [25]
    PAN J J, LI C L, LIU X J, et al. A multivariate insight into the organoleptic properties of porcine muscle by ultrasound-assisted brining: Protein oxidation, water state and microstructure[J]. LWT-Food Science and Technology,2022,159:113136. doi: 10.1016/j.lwt.2022.113136
    [26]
    CONTRERAS M, BENEDITO J, QUILES A, et al. Correction of defective textures in packaged dry-cured pork ham by applying conventional and ultrasonically-assisted mild thermal treatments[J]. LWT-Food Science and Technology,2020,126:109283. doi: 10.1016/j.lwt.2020.109283
    [27]
    BOUHRARA M, CLERJON S, DAMEZ J L, et al. Dynamic MRI and thermal simulation to interpret deformation and water transfer in meat during heating[J]. Journal of Agricultural and Food Chemistry,2011,59:1229−1235. doi: 10.1021/jf103384d
    [28]
    WU Z Y, BERTRAM H C, KOHLER A. Influence of aging and salting on protein secondary structures and water distribution in uncooked and cooked pork. A combined FT-IR micro spectroscopy and 1H NMR relaxometry study[J]. Journal of Agricultural and Food Chemistry,2006,54:8589−8597. doi: 10.1021/jf061576w
    [29]
    HAN Z Y, ZHANG J L, ZHENG J Y. The study of protein conformation and hydration characteristics of meat batters at various phase transition temperatures combined with low-field nuclear magnetic resonance and Fourier transform infrared spectroscopy[J]. Food Chemistry,2019,280:263−269. doi: 10.1016/j.foodchem.2018.12.071
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