Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal Experiment

SHAN Qianyi; SHEN Yueming; ZHANG Mingxing; TANG Jiacheng; CHEN Yanjie; BAO Jianqiang

doi:10.13386/j.issn1002-0306.2022030035

Volume 43 Issue 22

Nov. 2022

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Science and Technology of Food Industry > 2022 > 43(22): 256-264. > DOI: 10.13386/j.issn1002-0306.2022030035

SHAN Qianyi, SHEN Yueming, ZHANG Mingxing, et al. Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal Experiment[J]. Science and Technology of Food Industry, 2022, 43(22): 256−264. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030035.

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Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal Experiment

1.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
2.
Shanghai Jing'an District Market Supervision Administration, Shanghai 200072, China
3.
Ningbo Mingfeng Fishery Co., Ltd., Ningbo 315464, China
4.
Shanghai Egineering Research Center of Aquatic Product Processing and Preservation, Shanghai 201306, China
5.
Laboratory of Quality & Safety Risk Assessment for Aquatic Products Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China

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Received Date: March 02, 2022
Available Online: September 05, 2022

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Abstract

Abstract

In this study, the by-products of Chinese softshell turtle processing were used as raw materials, which provided the possibility to improve the extraction efficiency of Chinese softshell turtle oil and make it high-value utilization to provide a new source of profit for production enterprises. The extraction experiment of turtle oil was carried out by using ultrasonic combined with complex enzymatic hydrolysis (The pH of enzymatic hydrolysis was controlled at 7.5~8.5), and the extraction parameters were optimized by single factor experiments combined with orthogonal experiment, and the optimal extraction process conditions of ultrasonic-complex enzymatic hydrolysis method were obtained: The ratio of solid to liquid was 1:1.5 (g/mL), the amount of protease was 2.4%, the ratio of enzyme was 1:1.5 (neutral protease:alkaline protease), the enzymatic hydrolysis time was 2 h, the enzymatic hydrolysis temperature was 65 ℃, the ultrasonic power was 180 W, and the ultrasonic time was 30 min. The extraction rate of Chinese softshell turtle oil was 81.65%±0.62%. After the Chinese softshell turtle oil was refined, its physical and chemical indicators were all in line with the national aquatic industry standard (SC/T 3502-2016) refined fish oil first-class standard. At the same time, fatty acid composition analysis showed that the refined Chinese softshell turtle oil contained a total of 28 kinds of fatty acids, of which saturated fatty acids accounted for 23.81% of the total, monounsaturated fatty acids accounted for 46.94% of the total, polyunsaturated fatty acids accounted for 26.38% of the total, DHA and EPA content accounted for 10.05% of the total.
- by-products of Chinese softshell turtle processing,
- ultrasonic wave,
- compound enzyme,
- oil extraction,
- process optimization,
- fatty acids

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References (33)

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