Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal Experiment

SHAN Qianyi; SHEN Yueming; ZHANG Mingxing; TANG Jiacheng; CHEN Yanjie; BAO Jianqiang

doi:10.13386/j.issn1002-0306.2022030035

Volume 43 Issue 22

Nov. 2022

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Science and Technology of Food Industry > 2022 > 43(22): 256-264. > DOI: 10.13386/j.issn1002-0306.2022030035

SHAN Qianyi, SHEN Yueming, ZHANG Mingxing, et al. Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal Experiment[J]. Science and Technology of Food Industry, 2022, 43(22): 256−264. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030035.

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Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal Experiment

1.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
2.
Shanghai Jing'an District Market Supervision Administration, Shanghai 200072, China
3.
Ningbo Mingfeng Fishery Co., Ltd., Ningbo 315464, China
4.
Shanghai Egineering Research Center of Aquatic Product Processing and Preservation, Shanghai 201306, China
5.
Laboratory of Quality & Safety Risk Assessment for Aquatic Products Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China

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Received Date: March 02, 2022
Available Online: September 05, 2022

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Abstract

Abstract

In this study, the by-products of Chinese softshell turtle processing were used as raw materials, which provided the possibility to improve the extraction efficiency of Chinese softshell turtle oil and make it high-value utilization to provide a new source of profit for production enterprises. The extraction experiment of turtle oil was carried out by using ultrasonic combined with complex enzymatic hydrolysis (The pH of enzymatic hydrolysis was controlled at 7.5~8.5), and the extraction parameters were optimized by single factor experiments combined with orthogonal experiment, and the optimal extraction process conditions of ultrasonic-complex enzymatic hydrolysis method were obtained: The ratio of solid to liquid was 1:1.5 (g/mL), the amount of protease was 2.4%, the ratio of enzyme was 1:1.5 (neutral protease:alkaline protease), the enzymatic hydrolysis time was 2 h, the enzymatic hydrolysis temperature was 65 ℃, the ultrasonic power was 180 W, and the ultrasonic time was 30 min. The extraction rate of Chinese softshell turtle oil was 81.65%±0.62%. After the Chinese softshell turtle oil was refined, its physical and chemical indicators were all in line with the national aquatic industry standard (SC/T 3502-2016) refined fish oil first-class standard. At the same time, fatty acid composition analysis showed that the refined Chinese softshell turtle oil contained a total of 28 kinds of fatty acids, of which saturated fatty acids accounted for 23.81% of the total, monounsaturated fatty acids accounted for 46.94% of the total, polyunsaturated fatty acids accounted for 26.38% of the total, DHA and EPA content accounted for 10.05% of the total.
- by-products of Chinese softshell turtle processing,
- ultrasonic wave,
- compound enzyme,
- oil extraction,
- process optimization,
- fatty acids

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References

[1]	王丹, 吴反修, 宋丹丹, 等. 2021年中国渔业统计年鉴[M]. 北京: 中国农业出版社, 2020: 24−27 WANG D, WU F X, SONG D D, et al. 2021 China fishery statistical yearbook [M]. Beijing: China Agricultural Press, 2020: 24−27
[2]	MASON R, SHERRATT S. Eicosapentaenoic acid ( EPA) inhibits human low density lipoprotein oxidation in a con-centration and time dependent manner at pharmacologic doses in vitro[J/OL]. Atherosclerosis, 2019, 287: e80. https://www.atherosclero sis-journal.com/article/s0021-9150(19) 30687-2/fulltext.
[3]	孙林, 柏友萍, 林伟其. 脂肪因子Vaspin在运动与甲鱼油对老年肥胖大鼠胰岛素抵抗的作用[J]. 中国应用生理学杂志,2020,36(5):461−464. [SUN L, BO Y P, LIN W Q. Effects of adipokines Vaspin on exercise and turtle oil on insulin resistance in elderly obese rats[J]. Chinese Journal of Applied Physiology,2020,36(5):461−464. doi: 10.12047/j.cjap.5986.2020.098
[4]	RUBIO-RODRIGUEZ N, DE DIEGO S M, BELTRAN S, et al. Supercritical fluid extraction of fish oil from fish by-products: A comparison with other extraction methods[J]. Journal of Food Engineering,2012,109(2):238−248. doi: 10.1016/j.jfoodeng.2011.10.011
[5]	何秋实, 王志兴, 赵城彬. 超声辅助复合酶法制备核桃油工艺研究[J]. 中国油脂,2017,42(8):13−16. [HE Q S, WANG Z X, ZHAO C B. Study on the preparation of walnut oil by ultrasonic-assisted compound enzymatic method[J]. China Oils and Fats,2017,42(8):13−16. doi: 10.3969/j.issn.1003-7969.2017.08.004
[6]	叶茂, 邓毛程, 李静. 超声波辅助水酶法提取木棉籽油的工艺优化及性质研究[J]. 粮食与油脂,2021,34(5):63−67. [YE M, DENG M C, LI J. Study on process optimization and properties of ultrasonic-assisted water enzymatic extraction of kapok seed oil[J]. Grain and Oil,2021,34(5):63−67.
[7]	黄诚, 尹红. 超声波辅助复合溶剂提取柑橘皮油工艺条件优化[J]. 现代农业科技,2018(12):262−264. [HUANG C, YIN H. Optimization of ultrasonic-assisted compound solvent extraction process conditions for citrus peel oil[J]. Modern Agricultural Science and Technology,2018(12):262−264. doi: 10.3969/j.issn.1007-5739.2018.12.155
[8]	吴非, 李钊, 周琪, 等. 超声波辅助水酶法提取米胚油及其成分分析[J]. 食品科学,2020,41(24):233−241. [WU F, LI Z, ZHOU Q, et al. Ultrasonic-assisted water enzymatic extraction of rice germ oil and analysis of its components[J]. Food Science,2020,41(24):233−241. doi: 10.7506/spkx1002-6630-20200426-343
[9]	邓韦丝, 王敏, 吕秋冰. 复合酶法提取辣木籽油及其体外抗氧化活性[J]. 粮食与油脂,2019,32(12):67−72. [DENG W S, WANG M, LÜ Q B. Extraction of Moringa oleifera seed oil by compound enzymatic method and its antioxidant activity in vitro[J]. Food and Oil,2019,32(12):67−72.
[10]	高珊, 郝孟琪. 鲅鱼鱼油提取工艺研究[J]. 现代食品,2021(19):99−101. [GAO S, HAO M Q. Study on the extraction technology of mackerel fish oil[J]. Modern Food,2021(19):99−101. doi: 10.16736/j.cnki.cn41-1434/ts.2021.19.026
[11]	HOU Y, KEXIN M, YANG M, et al. Composition, characteristics and antioxidant activities of fruit oils from Idesia polycarpa using homogenate-circulating ultrasound-assisted aqueous enzymatic extraction[J]. Industrial Crops and Products,2018,138(34):235−237.
[12]	付雪媛, 钟宏, 宋文山, 等. 章鱼内脏鱼油的提取及品质分析[J]. 中国油脂,2020,45(5):17−22. [FU X Y, ZHONG H, SONG W S, et al. Extraction and quality analysis of fish oil from Octopus viscera[J]. China Oils and Fats,2020,45(5):17−22. doi: 10.12166/j.zgyz.1003-7969/2020.05.004
[13]	SÁNCHEZ-MACHADO D I, LÓPEZ-CERVANTES J, NÚÑEZ-GASTÉLUM J A, et al. Effect of the refining process on Moringa oleifera seed oil quality[J]. Food Chemistry,2015,187:53−57. doi: 10.1016/j.foodchem.2015.04.031
[14]	叶丽姿, 李佳, 潘英杰, 等. 鱿鱼加工副产物中油脂的综合利用研究进展[J]. 食品与机械,2021,37(10):215−221. [YE L Z, LI J, PAN Y J, et al. Research progress on comprehensive utilization of oils and fats in by-products of squid processing[J]. Food and Machinery,2021,37(10):215−221. doi: 10.13652/j.issn.1003-5788.2021.10.038
[15]	窦鑫. 大黄鱼鱼肝油的酶法制取与脱腥研究[D]. 上海: 上海海洋大学, 2021. DOU X. Study on the enzymatic preparation and deodorization of large yellow croaker liver oil[D]. Shanghai: Shanghai Ocean University, 2021.
[16]	王倩倩, 吕顺, 陆剑锋, 等. 酶法提取罗非鱼内脏鱼油及脂肪酸组成分析[J]. 中国粮油学报,2015(9):72−78. [WANG Q Q, LÜ S, LU J F, et al. Enzymatic extraction of tilapia visceral fish oil and analysis of fatty acid composition[J]. Chinese Journal of Cereals and Oils,2015(9):72−78. doi: 10.3969/j.issn.1003-0174.2015.09.014
[17]	谢丹, 李磊磊, 谢亮亮. 酶解技术在南极磷虾油提取中的应用研究进展[J]. 中国油脂,2021,46(4):133−138. [XIE D, LI L L, XIE L L. Research progress on the application of enzymolysis technology in the extraction of Antarctic krill oil[J]. China Oils and Fats,2021,46(4):133−138.
[18]	王妍, 崔红花, 綦振峰, 等. 酶解法从深海明太鱼内脏中提取鱼油工艺研究[J]. 延边大学农学学报,2013(2):147−151. [WANG Y, CUI H H, QI Z F, et al. Study on the extraction of fish oil from deep-sea plaice viscera by enzymatic hydrolysis[J]. Journal of Agronomy of Yanbian University,2013(2):147−151. doi: 10.3969/j.issn.1004-7999.2013.02.011
[19]	张丽娟, 郑碧远. 复合酶法提取鱿鱼内脏油工艺研究[J]. 食品工业,2018,39(5):123−126. [ZHANG L J, ZHENG B Y. Study on the extraction of squid visceral oil by compound enzyme method[J]. Food Industry,2018,39(5):123−126.
[20]	王文婷, 苏博, 杜丹丹, 等. 酶解法提取鲫鱼下脚料中鱼油的工艺研究[J]. 兰州文理学院学报(自然科学版),2019,33(3):48−52. [WANG W T, SU B, DU D D, et al. Research on the extraction of fish oil from crucian carp waste by enzymatic hydrolysis[J]. Journal of Lanzhou University of Arts and Sciences (Natural Science Edition),2019,33(3):48−52. doi: 10.13804/j.cnki.2095-6991.2019.03.010
[21]	王青, 王晓东, 程安玮, 等. 酶解预处理对水酶法提取牡丹籽油提油率的影响[J]. 中国食物与营养,2019,25(10):26−29. [WANG Q, WANG X D, CHENG A W, et al. The effect of enzymolysis pretreatment on the oil extraction rate of water-enzymatic extraction of peony seed oil[J]. China Food and Nutrition,2019,25(10):26−29. doi: 10.3969/j.issn.1006-9577.2019.10.005
[22]	夏蕴实, 刘畅, 王梓, 等. 复合酶水酶法提取鹿油及其脂肪酸组成分析[J]. 食品工业,2021,42(12):108−112. [XIA Y S, LIU C, WANG Z, et al. Extraction of deer oil by compound enzyme-water enzymatic method and analysis of its fatty acid composition[J]. Food Industry,2021,42(12):108−112.
[23]	CRAVOTTO G, BOFFA L, MANTEGNA S, et al. Improved extraction of vegetable oils under high-intensity ultrasound and/or microwaves[J]. Ultrasonics Sonochemistry,2008,15(5):898−902. doi: 10.1016/j.ultsonch.2007.10.009
[24]	JACOBS H, DELCOURJ A. Hydrothermal modifications of granular starch, with retention of the granular structure: A review[J]. J Agri Food Chem,1998,46(8):2895−2905. doi: 10.1021/jf980169k
[25]	OLIVEIRA G R, SANTOS A V, LIMA A S, et al. Neural modelling in adsorption column of cholesterol-removal efficiency from milk[J]. LWT-Food Science and Technology,2015,64:632−638. doi: 10.1016/j.lwt.2015.05.051
[26]	赵欣欣, 孔保华, 孙方达, 等. 功率超声对酶催化反应影响因素的研究进展[J]. 食品工业,2016,37(10):208−211. [ZHAO X X, KONG B H, SUN F D, et al. Research progress on factors affecting enzymatic catalysis by power ultrasound[J]. Food Industry,2016,37(10):208−211.
[27]	吕鹏, 庄重, 凌建亚, 等. 超声对酶的影响[J]. 生物技术通讯,2004(5):534−536. [LÜ P, ZHUANG Z, LING J Y, et al. Effect of ultrasound on enzymes[J]. Biotechnology Communications,2004(5):534−536. doi: 10.3969/j.issn.1009-0002.2004.05.032
[28]	李泽民, 何生林, 丁云桥, 等. 五因素混水平正交实验优化鱼鳞胶原提取工艺[J]. 山东化工,2019,48(20):18−22,24. [LI Z M, HE S L, DING Y Q, et al. Five-factor mixed-level orthogonal experiment to optimize the extraction process of fish scale collagen[J]. Shandong Chemical Industry,2019,48(20):18−22,24. doi: 10.3969/j.issn.1008-021X.2019.20.008
[29]	李利华. 正交实验法优选花椒多酚的超声波辅助提取工艺[J]. 中国调味品,2018,43(4):1−3,27. [LI L H. Optimization of ultrasonic-assisted extraction process of Zanthoxylum bungeanum polyphenols by orthogonal experimental method[J]. China Condiments,2018,43(4):1−3,27. doi: 10.3969/j.issn.1000-9973.2018.04.001
[30]	SHARMA A, CHAURASIA S P, DALAI A K. Enzymatic hydrolysis of cod liver oil for the fatty acids production[J]. Catalysis Today,2013,207(30):93−100.
[31]	RUBIO-RODR GUEZ N, DIEGO S D, BELTR N S, et al. Supercritical fluid extraction of the omega-3 rich oil contained in hake (Merluccius capensis-Merluccius paradoxus) by-products: Study of the influence of process parameters on the extraction yield and oil quality[J]. The Journal of Supercritical Fluids,2008,47(2):215−226. doi: 10.1016/j.supflu.2008.07.007
[32]	宋恭帅, 张蒙娜, 马永钧, 等. 大目金枪鱼加工副产物中鱼油提取制备及EPA分离纯化[J]. 核农学报,2019(6):1122−1130. [SONG G S, ZHANG M N, MA Y J, et al. Extraction and preparation of fish oil from by-products of bigeye tuna processing and separation and purification of EPA[J]. Chinese Journal of Nuclear Agriculture,2019(6):1122−1130. doi: 10.11869/j.issn.100-8551.2019.06.1122
[33]	于淑池, 李晨晨, 徐云升, 等. 金鲳鱼骨中鱼油的提取工艺及脂肪酸成分分析[J]. 食品工业,2021,42(3):147−152. [YU S C, LI C C, XU Y S, et al. Extraction process and fatty acid composition analysis of fish oil from golden pomfret bones[J]. Food Industry,2021,42(3):147−152.

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