WANG Weiya, LIU Yuting, WAN Hankun, et al. Correlation Analysis of Bacterial Community Structure and Biogenic Amines in Sufu[J]. Science and Technology of Food Industry, 2022, 43(22): 181−188. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030013.
Citation: WANG Weiya, LIU Yuting, WAN Hankun, et al. Correlation Analysis of Bacterial Community Structure and Biogenic Amines in Sufu[J]. Science and Technology of Food Industry, 2022, 43(22): 181−188. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030013.

Correlation Analysis of Bacterial Community Structure and Biogenic Amines in Sufu

More Information
  • Received Date: March 01, 2022
  • Available Online: September 01, 2022
  • In order to investigate the correlation of bacterial community structure and biogenic amines in sufu, Illumina Miseq high-throughput sequencing was used to analyze the bacterial community structure of sufu with different biogenic amines content and relevant environmental factors were determined. The results showed that Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes were the absolute dominant phyla and the relative abundance of the four phyla were more than 91% in sufu samples. Bacillus, Weissella, Tetragenococcus, and Pseudomonas were the main dominant genera in sufu. The main different genera between high and low biogenic amines content groups were Bacillus, Weissella, Pseudomonas and Lysinibacillus. Correlation analysis showed that biogenic amines and total acid were the main factors affecting bacterial community composition (P<0.05). The content of biogenic amines was mainly related to environmental factors such as amino acid nitrogen and pH. The content of amino acid nitrogen in sufu samples ranged from 0.62 to 0.86 g/100 g, and pH ranged from 5.62 to 6.37. Weissella, Bacillus and Lysinibacillus had a significant positive correlation with biogenic amines. Acinetobacter, Comamonas and Tetragenococcus had negtive correlation with biogenic amines. The research would elucidate the relationship between microbial community structure of sufu and biogenic amines, and provide a theoretical basis for understanding the formation mechanism of biogenic amines, effectively controlling biogenic amines and optimizing the production process of sufu.
  • [1]
    HAN B Z, CAO C F, ROMBOUTS F M, et al. Microbial changes during the production of Sufu–a Chinese fermented soybean food[J]. Food Control,2004,15(4):265−270. doi: 10.1016/S0956-7135(03)00066-5
    [2]
    ZHAO C J, SCHIEBER A, GAENZLE M G. Formation of taste-active amino acids, amino acid derivatives and peptides in food fermentations−a review[J]. Food Research International,2016,89(1):39−47.
    [3]
    MOY Y S, LU T J, CHOU C C. Volatile components of the enzyme-ripened sufu, a Chinese traditional fermented product of soy bean[J]. Journal of Bioscience & Bioengineering,2012,113(2):196−201.
    [4]
    LORET S, DELOYER P, DANDRIFOSSE G. Levels of biogenic amines as a measure of the quality of the beer fermentation process: Data from Belgian samples[J]. Food Chemistry,2005,89(4):519−525. doi: 10.1016/j.foodchem.2004.03.010
    [5]
    胡鹏, 索化夷, 阚建全, 等. 中国传统发酵豆豉中生物胺含量[J]. 食品科学,2013,34(20):108−112. [HU P, SUO H Y, KAN J Q, et al. Biogenic amine content of traditionally fermented Douchi[J]. Food Science,2013,34(20):108−112.
    [6]
    GU J, LIU T, HOU J, et al. Analysis of bacterial diversity and biogenic amines content during the fermentation processing of stinky tofu[J]. Food Research International,2018,111(3):689−698.
    [7]
    GUAN R F, LIU Z F, ZHANG J J. Investigation of biogenic amines in sufu (furu): A Chinese traditional fermented soybean food product[J]. Food Control,2013,31(2):345−352. doi: 10.1016/j.foodcont.2012.10.033
    [8]
    MAYER H K, GREGOR F. UHPLC analysis of biogenic amines in different cheese varieties[J]. Food Control, 2018, 93(3): 9−16.
    [9]
    KAMIL E, KHALID O A. The determination of some biogenic amines in Turkish fermented sausages consumed in Van[J]. Toxicology Reports,2018,5(4):639−643.
    [10]
    SANG X, MA X X, HAO H S, et al. Evaluation of biogenic amines and microbial composition in the Chinese traditional fermented food grasshopper sub shrimp paste[J]. LWT-Food Science and Technology,2020,134:109979. doi: 10.1016/j.lwt.2020.109979
    [11]
    QIU S, WAND Y, CHENG Y Q, et al. Reduction of biogenic amines in sufu by ethanol addition during ripening stage[J]. Food Chemistry,2018,239:1244−1252. doi: 10.1016/j.foodchem.2017.07.056
    [12]
    YANG B, TAN Y, KAN J Q. Regulation of quality and biogenic amine production during sufu fermentation by pure Mucor strains[J]. LWT-Food Science and Technology,2020,117(1):123−128.
    [13]
    SANTOS M H S. Biogenic amines: Their importance in foods[J]. International Journal of Food Microbiology,1996,29(2−3):213−231. doi: 10.1016/0168-1605(95)00032-1
    [14]
    李大伟, 李丹丹, 梁静静, 等. 高效液相色谱法测定市售腐乳中生物胺的含量[J]. 食品研究与开发, 2018, 39(16): 120−124, 129.

    LI D W, LI D D, LIANG J J, et al. Determination of biogenic amines in commercially sufu by high performance liquid chromatography[J]. Food Research and Development, 2018, 39(16): 120−124, 129.
    [15]
    杨熙, 颜婷婷, 殷丽君, 等. 腐乳中生物胺的产生及其控制研究进展[J]. 中国酿造,2021,40(10):1−6. [YANG X, YAN T T, YIN L J, et al. Research progress on production and control of biogenic amines in sufu[J]. China Brewing,2021,40(10):1−6. doi: 10.11882/j.issn.0254-5071.2021.10.001
    [16]
    李璇, 刘琪, 朱蔚姗, 等. 不同发酵豆制品中生物胺调查分析[J]. 食品安全质量检测学报, 2020, 11(1): 298−305.

    LI X, LIU Q, ZHU W S, et al. Investigation and analysis of biogenic amines in different fermented bean products[J]. Journal of Food Safety & Quality, 2020, 11(1): 298−305.
    [17]
    梁静静, 李大伟, 史瑞琴, 等. 腐乳中产生物胺菌株的筛选鉴定及产生物胺能力评价[J]. 河北农业大学学报,2019,42(3):88−93. [LIANG J J, LI D W, SHI R Q, et al. Screening and identification of biogenic amines producing strains from sufu and evaluation of biogenic amines production ability[J]. Journal of Hebei Agricultural University,2019,42(3):88−93. doi: 10.13320/j.cnki.jauh.2019.0061
    [18]
    王维亚, 黄瑞钰, 骆瑜, 等. 江西产腐乳生物胺水平调查及产胺菌的分离与鉴定[J]. 食品工业科技,2022,43(12):246−252. [WANG W Y, HUANG R Y, LUO Y, et al. Investigation of the biogenic amine content in Jiangxi-made sufu and isolation and identification of amine-producing bacteria[J]. Science and Technology of Food Industry,2022,43(12):246−252.
    [19]
    GUO Z, WANG Y R, XIANG F S, et al. Bacterial diversity in pickled cowpea (Vigna unguiculata [Linn.] Walp) as determined by Illumina MiSeq sequencing and culture-dependent methods[J]. Current Microbiology,2021,78(4):1286−1297. doi: 10.1007/s00284-021-02382-3
    [20]
    陶康, 吴凌伟, 金晓芳, 等. 基于高通量基因测序分析腐乳微生物多样性[J]. 食品科学,2021,42(8):143−149. [TAO K, WU L W, JIN X F, et al. Analysis of microbial diversity in sufu using high-throughput sequencing[J]. Food Science,2021,42(8):143−149.
    [21]
    刘振锋. 腐乳和臭干中生物胺的研究[D]. 杭州: 浙江大学, 2011.

    LIU Z F. Studies on biogenic amines in fermented soybean products: Sufu (furu) and stinky tofu (chougan)[D]. Hangzhou: Zhejiang University, 2011.
    [22]
    徐琼, 刘洋, 曲勤凤, 等. 高通量测序分析不同地区红腐乳细菌多样性[J]. 食品科学,2020,41(10):110−116. [XU Q, LIU Y, QU Q F, et al. High-throughput sequencing analysis of bacterial diversity in red sufu from different regions[J]. Food Science,2020,41(10):110−116. doi: 10.7506/spkx1002-6630-20190408-087
    [23]
    樊明涛. 发酵食品工艺学[M]. 北京: 科学出版社, 2017.

    FAN M T. Fermented food technology[M]. Beijing: Science Press, 2017.
    [24]
    LI Q, LI Y, LUO Y, et al. Shifts in diversity and function of the bacterial community during the manufacture of Fu brick tea[J]. Food Microbiology,2019,80(6):70−76.
    [25]
    李巧玉, 方芳, 堵国成, 等. 魏斯氏菌在发酵食品中的应用[J]. 食品与发酵工业,2017,43(10):241−247. [LI Q Y, FANG F, DU G C, et al. The application of Weissella strains in fermented food[J]. Food and Fermentation Industries,2017,43(10):241−247. doi: 10.13995/j.cnki.11-1802/ts.014934
    [26]
    YONG J W, YOUNG J J, JUNG L H, et al. Functional characterization of bacterial communities responsible for fermentation of doenjang: A traditional Korean fermented soybean paste[J]. Frontiers in Microbiology, 2016, 7: 827.
    [27]
    GUAN L, CHO K H, LEE J H. Analysis of the cultivable bacterial community in jeotgal, a Korean salted and fermented seafood, and identification of its dominant bacteria[J]. Food Microbiology,2011,28(1):101−113. doi: 10.1016/j.fm.2010.09.001
    [28]
    王博, 周朝晖, 李铁桥, 等. 嗜盐四联球菌及其在发酵食品中的应用[J]. 食品与发酵工业,2017,43(8):267−272. [WANG B, ZHOU C H, LI T Q, et al. Tetragenococcus halophilus and its application in fermented foods[J]. Food and Fermentation Industries,2017,43(8):267−272.
    [29]
    HAN B Z, WANG J H, ROMBOUTS F M, et al. Effect of NaCl on textural changes and protein and lipid degradation during the ripening stage of sufu, a Chinese fermented soybean food[J]. Journal of the Science of Food,2003,83(9):899−904. doi: 10.1002/jsfa.1425
    [30]
    李慧, 张建新. 傅立叶变换近红外光谱法测定腐乳中总酸、蛋白质和水分[J]. 分析试验室,2008(4):95−99. [LI H, ZHANG J X. Determination of total acid, protein and moisture contents in preserved bean curd using fourier transform near-infrared spectroscopy (FT-NIS)[J]. Chinese Journal of Analysis Laboratory,2008(4):95−99. doi: 10.3969/j.issn.1000-0720.2008.04.026
    [31]
    TAN Y, ZHANG R F, CHEN G J, et al. Effect of different starter cultures on the control of biogenic amines and quality change of douchi by rapid fermentation[J]. LWT-Food Science and Technology,2019,109:395−405. doi: 10.1016/j.lwt.2019.04.041
    [32]
    RIEBROY S, BENJAKUL S, VISESSANGUAN W, et al. Some characteristics and antioxidant activity of commercial sugars produced in Thailand[J]. Food Chemistry,2004,88(4):527−535. doi: 10.1016/j.foodchem.2004.01.067
    [33]
    李东蕊. 豆瓣酱工业发酵过程中生物胺的生成规律及微生物多样性的研究[D]. 无锡: 江南大学, 2020.

    LI D R. Study on the formation regularity of biogenic amines and microbial diversity during the industrial fermentation of broad bean paste[D]. Wuxi: Jiangnan University, 2020.
    [34]
    SHUKLA S, PARK H K, LEE J S, et al. Reduction of biogenic amines and aflatoxins in Doenjang samples fermented with various Meju as starter cultures[J]. Food Control,2014,42:181−187. doi: 10.1016/j.foodcont.2014.02.006
    [35]
    TAKEBE Y, TAKIZAKI M, TANAKA H, et al. Evaluation of the biogenic amine-production ability of lactic acid bacteria isolated from Tofu-misozuke[J]. Food Science and Technology Research,2016,22(5):673−678. doi: 10.3136/fstr.22.673
    [36]
    JEONG D W, LEE J H. Antibiotic resistance, hemolysis and biogenic amine production assessments of Leuconostoc and Weissella isolates for kimchi starter development[J]. LWT-Food Science and Technology,2015,64(2):1078−1084. doi: 10.1016/j.lwt.2015.07.031
    [37]
    胡翠翠. 黄酒曲中微生物菌群结构分析及生物胺产生菌的分离鉴定[D]. 天津: 天津科技大学, 2017.

    HU C C. Analysis of microbial flora structure, isolation and identification biogenic amines producing bacteria of rice wine qu[D]. Tianjin: Tianjin University of Science and Technology, 2017.
    [38]
    刘爱芳, 谢晶, 钱韻芳. 冷藏金枪鱼优势腐败菌致腐败能力[J]. 食品科学,2018,39(3):7−14. [LIU A F, XIE J, QIAN Y F. Spoilage potential of dominant spoilage bacteria from chilled tuna (Thunnus obesus)[J]. Food Science,2018,39(3):7−14. doi: 10.7506/spkx1002-6630-201803002
    [39]
    JEONG D W, HUR S, LEE J H. Safety assessment of Tetragenococcus halophilus isolates from doenjang, a Korean high-salt-fermented soybean paste[J]. Food Microbiology,2017,62(4):92−98.
    [40]
    KUDA T, IZAWA Y, ISHII S, et al. Suppressive effect of Tetragenococcus halophilus, isolated from fish-nukazuke, on histamine accumulation in salted and fermented fish[J]. Food Chemistry,2012,130(3):569−574. doi: 10.1016/j.foodchem.2011.07.074
    [41]
    LI J, HUANG J, YAO J, et al. Aflatoxin B1 degradation by salt tolerant Tetragenococcus halophilus CGMCC 3792[J]. Food and Chemical Toxicology,2018,121(11):430−436.
    [42]
    王强, 周真江, 曾维友, 等. 生物胺降解乳酸菌的筛选与特性研究[J]. 中国酿造,2021,40(3):115−119. [WANG Q, ZHOU Z J, ZENG W Y, et al. Screening and characteristics of lactic acid bacteria degrading biogenic amines[J]. China Brewing,2021,40(3):115−119. doi: 10.11882/j.issn.0254-5071.2021.03.021
    [43]
    HUANG X N, YU S Z, HAN B Z, et al. Bacterial community succession and metabolite changes during sufu fermentation[J]. LWT-Food Science and Technology,2018,97:537−545. doi: 10.1016/j.lwt.2018.07.041
    [44]
    孙霞, 杨勇, 巩洋, 等. 发酵香肠中产生物胺的微生物及其检测方法研究[J]. 食品工业科技,2015,36(12):379−384. [SUN X, YANG Y, KONG Y, et al. Review on microorganism of producing biogenic amine and detecting methods in fermented sausage[J]. Science and Technology of Food Industry,2015,36(12):379−384.
    [45]
    王新南. 发酵豆制品中生物胺含量研究进展[J]. 中国调味品,2019,44(9):188−190. [WANG X N. Research progress of biogenic amines in fermented soybean products[J]. China Condiment,2019,44(9):188−190. doi: 10.3969/j.issn.1000-9973.2019.09.041
  • Cited by

    Periodical cited type(7)

    1. 陈雪花,陈建平,罗宝浈,李佳睿,李瑞,刘晓菲,宋兵兵,钟赛意. 硫酸软骨素纳米硒的结构表征及其对Hela细胞迁移和侵袭的影响. 食品与发酵工业. 2024(03): 73-79 .
    2. 赵猛,丁子康,李欣悦,王晓梅,胡祖广,张忠山. 低分子量坛紫菜多糖纳米硒的制备、表征及其体外抗氧化活性. 食品工业科技. 2024(23): 170-178 . 本站查看
    3. 向东,朱玉昌,周大寨,李爽. 含硒活性物质研发技术进展. 山东化工. 2023(05): 66-69+77 .
    4. 王鑫,周卓,王峙力,修伟业,罗钰,马永强. 硒化甜玉米芯多糖对非酶糖基化的抑制作用. 食品工业科技. 2023(19): 17-23 . 本站查看
    5. 徐孝楠,马浩迪,续炎,李璇,覃智,权春善,张丽影. 耐硒海洋菌株的筛选、鉴定及其产纳米硒的抗菌活性. 食品工业科技. 2023(24): 152-158 . 本站查看
    6. 陈博文,陈建平,黄文浩,钟赛意,李瑞,宋兵兵,刘晓菲,汪卓. 岩藻多糖纳米硒的制备及其抑制肿瘤细胞增殖的研究. 天然产物研究与开发. 2023(12): 2117-2126 .
    7. 向文杰,殷彩桥,黄慧,陈婷. 硒及硒化合物对食管癌作用机制研究进展. 社区医学杂志. 2022(22): 1295-1300 .

    Other cited types(5)

Catalog

    Article Metrics

    Article views (254) PDF downloads (20) Cited by(12)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return