Optimization Extraction of Pectin from Ganzhou Navel Orange Peel and Investigation of Its Physicochemical Properties and Biological Activities before and after Modification

DU Chao; LIU Jiaci; CAO Manyu; ZUO Feng; ZANG Yanqing

doi:10.13386/j.issn1002-0306.2022020242

Volume 43 Issue 21

Oct. 2022

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Science and Technology of Food Industry > 2022 > 43(21): 235-244. > DOI: 10.13386/j.issn1002-0306.2022020242

DU Chao, LIU Jiaci, CAO Manyu, et al. Optimization Extraction of Pectin from Ganzhou Navel Orange Peel and Investigation of Its Physicochemical Properties and Biological Activities before and after Modification[J]. Science and Technology of Food Industry, 2022, 43(21): 235−244. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022020242.

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Optimization Extraction of Pectin from Ganzhou Navel Orange Peel and Investigation of Its Physicochemical Properties and Biological Activities before and after Modification

College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China

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Received Date: February 27, 2022
Available Online: August 21, 2022

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Abstract

Abstract

In order to realize the resource utilization of Ganzhou navel orange peel, the pectin (GOP) was extracted by ultrasonic-microwave synergistic extraction from Ganzhou navel orange peel. Box-behnken experiment was used to optimize the process parameters. The modified pectin (MGOP) was obtained by pH modification of GOP. The changes of physicochemical properties of GOP and MGOP were evaluated. On this basis, the antioxidant activities and their effects on corn starch gelatinization and in vitro digestion were studied. The results showed that the highest yield of GOP (18.25%±0.31%) was observed under optimal condition (microwave power 550 W, microwave time 30 min, pH2.4, liquid-solid ratio 25.00 mL•g^-1. After modification, the esterification degree of pectin decreased from 74.10%±2.73% to 33.27%±2.47%, the content of galacturonic acid increased from 74.65%±1.11% to 88.19%±2.98%, significantly. GOP and MGOP showed good antioxidant activity. After modification, the antioxidant capacity of pectin was enhanced. When the concentration of MGOP was 8 mg/mL, the scavenging rate of DPPH and ABTS free radicals reached 84.35%±0.28% and 87.33±0.43%, respectively. Adding 6% GOP and 6% MGOP could significantly increase SDS to 32.05%±0.65% and 43.83%±0.48%, and RS to 14.61%±1.40% and 19.84%±2.76%, respectively. In addition, the addition of pectin also increased the proportion of small particles in starch paste, inhibited the swelling of starch particles, and thus inhibited the digestion of starch. Compared with GOP, MGOP had more significant effects. The results of this study provided theoretical basis and technical support for the development and utilization of Ganzhou navel orange peel pectin.
- Ganzhou navel orange peel pectin,
- pH-modification,
- physicochemical properties,
- antioxidant ability,
- in vitro digestion

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