Optimization of Extraction Method of Arabinoxylan from Maize Bran and Analysis of Its Gel Properties

XIAO Yu; ZHANG Tao; CHEN Aijun; XU Xiaoqi; LI Sha; SUN Guilian; LI Kewen; XU Hong

doi:10.13386/j.issn1002-0306.2022020191

Volume 44 Issue 15

Jul. 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(15): 166-174. > DOI: 10.13386/j.issn1002-0306.2022020191

XIAO Yu, ZHANG Tao, CHEN Aijun, et al. Optimization of Extraction Method of Arabinoxylan from Maize Bran and Analysis of Its Gel Properties[J]. Science and Technology of Food Industry, 2023, 44(15): 166−174. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022020191.

Citation:

PDF (3463 KB)

Optimization of Extraction Method of Arabinoxylan from Maize Bran and Analysis of Its Gel Properties

1.
College of Food and Light Industry, Nanjing Tech University, Nanjing 211816, China
2.
College of Food Science and Engineering, Nanjing University of Finance and Economics, Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, China
3.
Baolingbao Biology Co., Ltd., Dezhou 251200, China

More Information

Received Date: February 21, 2022
Available Online: June 05, 2023

Graphical Abstract

Abstract

Abstract

In order to further explore the added value of corn bran and improve the processing and application of AX, this study used the corn bran as raw material, optimized its extraction process by alkaline preparation, and explored its gel characteristics. AX was extracted from bran, a by-product of deep processing of corn, by using Ba(OH)₂. A response surface optimization with three factors and three levels was constructed based on the ratio of material to liquid, extraction temperature and extraction time. The structure of the optimized polysaccharide was characterized by monosaccharide composition, molecular weight and ferulic acid content. On this basis, AX was oxidized and cross-linked by laccase to explore the formation conditions, gel structure and thermal stability of AX gel. The results showed that the optimized extraction conditions of AX were as follows: Solid-liquid ratio 1:44 (g:mL), extraction temperature 69 ℃, extraction time 6.3 h, and the yield of AX was 23.07%±1.15%. The obtained AX was mainly composed of arabinose and xylose. The Ara/Xyl ratio was 2.28±0.11, the weight average molecular weight was 265±6.68 kDa, and the ferulic acid content was 9.6±0.32 mg/100 g. AX could form a stable gel structure under the condition of pH2.0 after laccase oxidation and cross-linking. The microstructure of AX was a dense porous gel structure and had good thermal stability according to scanning electron microscope. Arabinoxylan and its gel prepared in this study had good physical and chemical properties, and had potential application in improving the texture quality of food, embedding and controlled release of functional factors. This paper provides a theoretical basis for the further processing of corn by-products and the application of arabinoxylan in food industry.
- maize bran,
- arabinoxylans,
- optimization of extraction process,
- structure analysis,
- gel properties

FullText(HTML)

References (31)

References

[1]	刘玉春, 孙庆杰. 工业玉米副产品玉米皮精深加工技术进展[J]. 农产品加工,2017(2):72−75. [LIU Yuchun, SUN Qingjie. Progress in processing technology of corn husk-the byproduct of corn starch[J]. Farm Products Processing,2017(2):72−75. doi: 10.16693/j.cnki.1671-9646(X).2017.02.022 LIU Yuchun, SUN Qingjie. Progress in processing technology of corn husk-the byproduct of corn starch[J]. Farm Products Processing, 2017(2): 72-75. doi: 10.16693/j.cnki.1671-9646(X).2017.02.022
[2]	王文侠, 尚庆, 李馥邑, 等. 纤维素酶法提取玉米麸皮多糖的工艺条件优化[J]. 中国食品添加剂,2016,145(3):47−56. [WANG Wenxia, SHANG Qing, LI Fuyi, et al. Optimization of extraction of polysaccharides from corn fiber by celluclast[J]. China Food Additives,2016,145(3):47−56. doi: 10.3969/j.issn.1006-2513.2016.03.005 WANG Wenxia, SHANG Qing, LIFu-yi, et al. Optimization of extraction of polysaccharides from corn fiber by celluclast[J]. China Food Additives, 2016, 145(3): 47-56. doi: 10.3969/j.issn.1006-2513.2016.03.005
[3]	CHANLIAUD E, SAULNIER L, THIBAULT J F. Alkaline extraction and characterisation of heteroxylans from maize bran[J]. Journal of Cereal Science,1995,21(2):195−203. doi: 10.1016/0733-5210(95)90035-7
[4]	ROBERTO C, GIULIA S, ALESSANDRO C, et al. Variations in content and extractability of durum wheat (Triticum turgidum L. var durum) arabinoxylans associated with genetic and environmental factors[J]. International Journal of Molecular Sciences,2011,12(7):4536−4549. doi: 10.3390/ijms12074536
[5]	王立博, 陈复生, 殷丽君. 阿拉伯木聚糖结构特性及生理功能研究进展[J]. 食品工业,2016,37(8):211−215. [WANG Libo, CHEN Fusheng, YIN Lijun. Research progress in structural properties and physiological functions of arabinoxylans[J]. Food Industry,2016,37(8):211−215. WANG Libo, CHEN Fusheng, YIN Lijun. Research progress in structural properties and physiological functions of arabinoxylans[J]. Food Industry, 2016, 37(8): 211-215.
[6]	IZYDORCZYK M S, BILIADERIS C G. Cereal arabinoxylans: Advances in structure and physicochemical properties[J]. Carbohydrate Polymers,1995,28(1):33−48. doi: 10.1016/0144-8617(95)00077-1
[7]	KAUR A, YADAV M P, SINGH B, et al. Isolation and characterization of arabinoxylans from wheat bran and study of their contribution to wheat flour dough rheology[J]. Carbohydrate Polymers,2019,221:166−173. doi: 10.1016/j.carbpol.2019.06.002
[8]	SAULNIER L, GUILLON F, CHATEIGNER-BOUTIN A L. Cell wall deposition and metabolism in wheat grain[J]. Jourmnal of Cereal Science,2012,56(1):91−108. doi: 10.1016/j.jcs.2012.02.010
[9]	BERLANGA-REYES C M, CARVAJAL-MILLAN E, HICKSK B, et al. Protein/arabinoxylans gels: Effect of mass ratio on the rheological, microstructural and diffusional characteristics[J]. International Journal of Molecular Sciences,2014,15(10):19106−19118. doi: 10.3390/ijms151019106
[10]	KHAN M, HAIDER S, RAZA M, et al. Smart and pH-sensitive rGO/Arabinoxylan/chitosan composite for wound dressing: In-vitro drug delivery, antibacterial activity, and biological activities[J]. International Journal of Biological Macromolecules,2021,192(1):820−831.
[11]	刘成龙, 殷丽君, 陈复生, 等. 阿拉伯木聚糖凝胶的制备与应用研究进展[J]. 食品科技,2016,41(11):214−218. [LIU Chenglong, YIN Lijun, CHEN Fusheng, et al. Review on the preparation and application of arabinoxylan gel[J]. Food Science and Technology,2016,41(11):214−218. doi: 10.13684/j.cnki.spkj.2016.11.047 LIU Chenglong, YIN Lijun, CHEN Fusheng, et al. Review on the preparation and application of arabinoxylan gel[J]. Food Science and Technology, 2016, 41(11): 214-218. doi: 10.13684/j.cnki.spkj.2016.11.047
[12]	徐启恩. 超声辅助酶法提取麦麸阿拉伯木聚糖的工艺优化[D]. 郑州: 河南工业大学, 2017 XU Qien. Optimization of ultrasound combined with enzymatic extraction of arabinoxylan from wheat bran[D]. Zhengzhou: Henan University of Technology, 2017.
[13]	姜玉莹. 玉米麸皮阿拉伯木聚糖的提取、纯化及抗炎活性研究[D]. 长春: 吉林农业大学, 2019 JIANG Yuying. Extraction, purification and anti-inflammatory activity of corn bran arabinoxylan[D]. Changchun: Jilin Agricultural University, 2019.
[14]	蔡天革, 侯倩倩, 唐凤德. 燕麦麸皮β-葡聚糖的提取优化研究[J]. 辽宁大学学报,2017,44(4):351−356. [CAI Tiange, HOU Qianqian, TANG Fengde. Rearch of extraction and optimization of oat bran β-glucan[J]. Journal of Liaoning University,2017,44(4):351−356. CAI Tiange, HOU Qianqian, TANG Fengde. Rearch of extraction and optimization of oat bran β-glucan[J]. Journal of Liaoning University, 2017, 44(4): 351-356.
[15]	李勤勤, 耿欣. 玉米麸皮不同处理及其成分分析[J]. 食品研究与开发,2012,33(8):5. [LI Qinqin, GENG Xin. Different processes of corn bran and determination of main components[J]. Food Research and Development,2012,33(8):5. doi: 10.3969/j.issn.1005-6521.2012.08.011 LI Qinqin, GENG Xin. Different processes of corn bran and determination of main components[J]. Food Research and Development, 2012, 33(8): 5. doi: 10.3969/j.issn.1005-6521.2012.08.011
[16]	黎芳, 刘佳, 王冉冉, 等. 小麦麸皮水不溶性阿拉伯木聚糖提取及其酶解产物分析[J]. 中国酿造,2019,38(2):122−126. [LI Fang, LIU Jia, WANG Ranran, et al. Extraction of water insoluble arabinoxylan from wheat bran and analysis of its enzymatic hydrolyzed products[J]. China Brewing,2019,38(2):122−126. doi: 10.11882/j.issn.0254-5071.2019.02.023 LI Fang, LIU Jia, WANG Ranran, et al. Extraction of water insoluble arabinoxylan from wheat bran and analysis of its enzymatic hydrolyzed products[J]. China Brewing, 2019, 38(2), 122-126. doi: 10.11882/j.issn.0254-5071.2019.02.023
[17]	ZHANG Xiaowei, CHEN Tingting, LI Jongbin, et al. Acid gelation of soluble laccase-crosslinked corn bran arabinoxylan and possible gel formation mechanism[J]. Food Hydrocolloids,2019,92:1−9. doi: 10.1016/j.foodhyd.2019.01.032
[18]	张晓娜. 小麦麸皮中阿拉伯木聚糖的提取及生理活性研究[D]. 北京: 中国农业大学, 2007 ZHANG Xiaona. Study on the extraction and physiological activity of arabinoxylan from wheat bran[D]. Beijing: China Agricultural University, 2007.
[19]	COURTIN C M, DELCOUR J A. Arabinoxylans and endoxylanases in wheat flour bread-making[J]. Journal of Cereal Science,2002,35(3):225−243. doi: 10.1006/jcrs.2001.0433
[20]	徐中香. 青稞麸皮阿拉伯木聚糖的结构与溶液特性[D]. 上海: 上海交通大学, 2018 [XU Zhongxiang. Structure and solution prop extracts of arabinoxylans from hull-less barley bran[D]. Shanghai: Shanghai Jiao Tong University, 2018.
[21]	王伟旭, 汪丽萍, 于雷, 谭斌. 提取条件对麸皮水不溶性阿拉伯木聚糖得率及性质的影响[J]. 中国食品学报,2018,18(10):163−169. [WANG Weixu, WANG Liping, YU Lei, et al. Effect of extraction conditions on the water-insoluble arabinoxylan from wheat bran yield and properties[J]. Journal of Chinese Institute of Food Science and Technology,2018,18(10):163−169. doi: 10.16429/j.1009-7848.2018.10.021 WANG Weixu, WANG Liping, YU Lei, et al. Effect of extraction conditions on the water-insoluble arabinoxylan from wheat bran yield and properties[J]. Journal of Chinese Institute of Food Science and Technology, 2018, 18(10): 163-169. doi: 10.16429/j.1009-7848.2018.10.021
[22]	周启静. 小米糠阿拉伯木聚糖的制备及其功能性质的研究[D]. 南京: 南京农业大学, 2017 ZHOU Qijing. Sturdy on preparation and functional properties of arabinoxylan of milley bran[D]. Nanjing: Nanjing Agricultural University, 2017.
[23]	田贝贝, 陈洁, 王远辉. 小麦淀粉加工废水中阿拉伯木聚糖的理化性质及抗氧化活性研究[J]. 食品工业科技,2017,15:40−44. [TIAN Beibei, CHEN Jie, WANG Yuanhui. Study on physicochemical properties and antioxidant activity of arabinoxylan from wheat starch wastewater[J]. Science and Technology of Food Industry,2017,15:40−44. doi: 10.13386/j.issn1002-0306.2017.15.009 TIAN Beibei, CHEN Jie, WANG Yuanhui. Study on physicochemical properties and antioxidant activity of arabinoxylan from wheat starch wastewater [J]. Science and Technology of Food Industry, 2017, 15: 40-44. doi: 10.13386/j.issn1002-0306.2017.15.009
[24]	CARVAJAL-MILLAN E, STÉPHANE G, MARIE-HÉLÈNE M, et al. Impact of the structure of arabinoxylan gels on their rheological and protein transport properties[J]. Carbohydrate Polymers,2005,60(4):431−438. doi: 10.1016/j.carbpol.2005.02.014
[25]	王立博, 陈复生, 殷丽君, 等. 小麦阿拉伯木聚糖水凝胶结构及流变特性[J]. 食品科学,2017,38(13):41−46. [WANG Libo, CHEN Fusheng, YIN Lijun, et al. Structure and rheological properties of wheat bran arabinoxylan hydrogel[J]. Food Science,2017,38(13):41−46. doi: 10.7506/spkx1002-6630-201713007 WANG Libo, CHEN Fusheng, YIN Lijun, et al. Structure and rheological properties of wheat bran arabinoxylan hydrogel[J]. Food Science, 2017, 38(13): 41-46. doi: 10.7506/spkx1002-6630-201713007
[26]	黄曹兴, 何娟, 闵斗勇, 等. 毛竹竹青和竹黄半纤维素的提取与结构表征[J]. 林产化学与工业,2015,35(5):29−36. [HUANG Caoxing, HE Juan, MIN Douyong, et al. Isolation and characterization of hemicellulose from moso bamboo green and bamboo yellow[J]. Chemistry and Industry of Forest Products,2015,35(5):29−36. doi: 10.3969/j.issn.0253-2417.2015.05.006 HUANG Caoxing, HE Juan, MIN Douyong, et al. Isolation and characterization of hemicellulose from moso bamboo green and bamboo yellow [J]. Chemistry and Industry of Forest Products, 2015, 35(5): 29-36. doi: 10.3969/j.issn.0253-2417.2015.05.006
[27]	GAO C D, REN J L, ZHAO C, et al. Xylan-based temperature/pH sensitive hydrogels for drug controlled release[J]. Carbohydrate Polymers,2016,151:189−197. doi: 10.1016/j.carbpol.2016.05.075
[28]	ROSE D J, PATTERSON J A, HAMAKER B R. Structural differences among alkali-soluble arabinoxylans from maize (Zea mays), rice (Oryza sativa), and wheat (Triticum aestivum) brans influence human fecal fermentation profiles[J]. Journal of Agricultural & Food Chemistry,2010,58(1):493−499.
[29]	杨桔. 多孔P(NIPAm-CO-AAm)水凝胶对蛋白质控制释放的研究[J]. 功能材料,2013,44(21):4. [YANG Ju. Study on drug releasing properties of porous P(NIPAm-CO-AAm)hydrogels[J]. Journal of Functional Materials,2013,44(21):4. YANG Ju. Study on drug releasing properties of porous P(NIPAm-CO-AAm)hydrogels. Journal of Functional Materials, 2013, 44(21): 4.
[30]	COZIC C, PICTON L, GARDA M R, et al. Analysis of arabic gum: Study of degradation and water desorption processes[J]. Food Hydrocolloids,2009,23(7):1930−1934. doi: 10.1016/j.foodhyd.2009.02.009
[31]	MOTHÉ C G, RAO M A. Thermal behavior of gum arabic in comparison with cashew gum[J]. Thermochimica Acta,2000,357:9−13.

Supplements (0)

Cited By

Cited by

Periodical cited type(18)

1.	蒯振彧，吴凌燕，曾宣，朱正飞，孟艳秋. 基于PTK靶点的黄芪中黄酮化合物抗肿瘤筛选及活性研究. 沈阳药科大学学报. 2025(02): 162-169 .
2.	崔瑞芳，李仁廷，纪长隆，高孔雀，张亚茹. 沙参麦冬汤治疗肺癌临床及基础研究进展. 辽宁中医药大学学报. 2024(04): 211-216 .
3.	李兴强，晁旭，冯雪松. 槲皮素对肝细胞性肝癌的增效减毒效应研究进展. 世界科学技术-中医药现代化. 2024(02): 287-293 .
4.	周锋，沙德胜，卢瑗瑗，陈维. 槲皮素提高人结直肠癌细胞SW480和SW620对顺铂的敏感性. 西安交通大学学报(医学版). 2024(06): 902-908 .
5.	朱陆姣，王海波，姜宁祖，王文安，庄家圆，周珩. 槲皮素对胃癌的抑制作用及免疫调节机理研究. 西部中医药. 2024(11): 6-12 .
6.	李丹，汪鸣霄，周洵. 基于网络药理学探讨加减消瘰丸治疗肺结节病的作用机制. 贵州科学. 2024(06): 27-32 .
7.	邢文雅，刘锦梅，尹鑫东，周源子，寇世禄，黄麟琅，曹仕兵. 基于数据挖掘和网络药理学探讨中药治疗肠痈的用药规律及作用机制. 湖南中医杂志. 2024(12): 34-43 .
8.	张立，周继红，谢嘉嘉，焦小强，李珏卉，何逸龙. 淫羊藿-巴戟天治疗新型冠状病毒感染肺肾两虚型的网络药理学分析. 现代医药卫生. 2023(03): 404-410+414 .
9.	李雪，李俐，王小龙，郝利民，刘永奇，刘可可，张雪梅，鲁吉珂，伊娟娟. 多酚类化合物基于非编码RNA调控发挥抗肿瘤及辐射增敏作用研究进展. 食品科学. 2023(05): 222-230 .
10.	潘晔，符诚，王欣，张明利. 基于数据挖掘和网络药理学探究新型冠状病毒感染治疗方的组方规律及作用机制. 中医临床研究. 2023(14): 10-20 .
11.	程菲儿，要子妍，云少君，曹瑾玲，程艳芬，冯翠萍. 荞麦槲皮素改善脂代谢异常肝原代细胞的作用机制. 食品研究与开发. 2023(15): 70-75 .
12.	Can Huang，Ling Yuan，Yang Niu，Ya-Ting Yang，Yi-Fan Yang，Yi Nan，Hong-Li Dou，Joanna Japhet. Using Network Pharmacology and Molecular Docking Tools to Investigate the Potential Mechanism of Ephedra-Gypsum in the Treatment of Respiratory Diseases. World Journal of Traditional Chinese Medicine. 2023(02): 150-159 .
13.	林丽彬，谢群，邱新悦，吴梦羽，徐丽，张静霞. 槲皮素经抑制己糖激酶活性介导人肝癌Huh-7细胞的增殖、侵袭和转移. 中华实验外科杂志. 2023(10): 1951-1954 .
14.	张欣，刘峥，张颖，郭永胜，李建章，高强. 环氧化槲皮素合成及其对豆胶性能的影响. 林业工程学报. 2022(05): 87-92 .
15.	董敬，彭小芸，付西，任益锋，张龙飞，李林炯，祝捷，由凤鸣. 基于数据挖掘和网络药理学的中医药治疗肺结节用药规律及作用机制分析. 中草药. 2022(20): 6544-6557 .
16.	王博，覃富强，邓凤莹，罗惠格，陈祥飞，成果，白扬，黄小云，韩佳宇，曹雄军，白先进. ‘阳光玫瑰’葡萄一年两收果实类黄酮组分及含量差异分析. 中国农业科学. 2022(22): 4473-4486 .
17.	李旭东，吴静澜，田孟斌，聂琴. 高粱泡叶化学成分的UHPLC-Q Exactive Focus MS/MS分析. 湖北民族大学学报(医学版). 2022(04): 18-22 .
18.	邢智芹. 槲皮素生物学功能及其对动物生产与经济效益的影响研究进展. 饲料研究. 2022(24): 155-159 .