AuNPs Enhanced Microwave Coupled Lipase Synthesis of Starch Oleate Ester

WANG Yan; LI Hongjia; CHENG Meijia; XIE Jinhui; LIU Tianjiao; XIN Jiaying; ZHANG Na

doi:10.13386/j.issn1002-0306.2022020183

Volume 43 Issue 23

Nov. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(23): 200-209. > DOI: 10.13386/j.issn1002-0306.2022020183

WANG Yan, LI Hongjia, CHENG Meijia, et al. AuNPs Enhanced Microwave Coupled Lipase Synthesis of Starch Oleate Ester[J]. Science and Technology of Food Industry, 2022, 43(23): 200−209. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022020183.

Citation:

PDF (3082 KB)

AuNPs Enhanced Microwave Coupled Lipase Synthesis of Starch Oleate Ester

Key Laboratory of Food Science and Engineering, Harbin University of Commerce, Harbin 150028, China

More Information

Received Date: February 21, 2022
Available Online: October 08, 2022

Graphical Abstract

Abstract

Abstract

The nano-gold immobilized lipase (CAL@AuNPs) was prepared to synthesize starch oleate ester efficiently by using pre-treated corn starch and oleic acid as raw materials. Optimisation of the preparation conditions of CAL@AuNPs was carried out using oleic acid conversion rate as an indicator. The substitution degree was used as an indicator to investigate the catalytic efficiency of free enzyme, commercial immobilised enzyme and nanogold immobilised lipase under microwave-assisted conditions. The experimental results showed that the immobilized lipase with the strongest catalytic activity could be obtained by using AuNPs of 14 nm as carrier at 40 ℃ for 6 h. The starch oleate ester with the highest substitution degree of 0.0259 could be prepared by using CAL@AuNPs as catalyst under the condition of 400 W, 35 ℃ for 40 min. Compared with free enzyme, and commercial immobilized enzyme , the CAL@AuNPs could shorten reaction time and improve the degree of substitution.

FullText(HTML)

References (29)

References

[1]	MASINA N, CHOONARA Y E, KUMAR P, et al. A review of the chemical modification techniques of starch[J]. Carbohydrate Polymers,2016,157:1−11.
[2]	OTACHE M A, DURU U R, ACHUGASIM O, et al. Advances in the modification of starch via esterification for enhanced properties[J]. Journal of Polymers and the Environment,2021,29(5):1365−1379. doi: 10.1007/s10924-020-02006-0
[3]	OLTRAMARI K, MADRONA G S, ANDRÉ MONGE NETO, et al. Citrate esterified cassava starch: Preparation, physicochemical characterisation, and application in dairy beverages[J]. Starch-Stärke,2017,69(11-12):1−26.
[4]	YAN H, LU Q. Physicochemical properties of starch-wheat germ oil complex and its effects on water distribution and hardness of noodles[J]. LWT,2020(135):1−7.
[5]	ASHOGBON A O. Dual modification of various starches: Synthesis, properties and applications[J]. Food Chemistry,2020,342(11):1−38.
[6]	AMARAWEERA S M, GUNATHILAKE C, GUNAWARDENE O, et al. Development of starch-based materials using current modification techniques and their applications: A review[J]. Molecules (Basel, Switzerland),2021,26(22):6880. doi: 10.3390/molecules26226880
[7]	聂卉, 闵玉涛, 李玉玲, 等. 阿魏酸淀粉酯制备与表征及其对馒头预发酵冷冻面团物化性质的影响研究[J]. 中国食品添加剂,2021,32(11):123−128. [NIE H, MIN Y T, LI L Y, et al. Effect of starch ferulate on physicochemical properties of prefermented frozen dough and steamed bread[J]. China Food Additives,2021,32(11):123−128. doi: 10.19804/j.issn1006-2513.2021.11.017
[8]	ZHANG K, CHENG F, ZHANG K, et al. Synthesis of long-chain fatty acid starch esters in aqueous medium and its characterization[J]. European Polymer Journal,2019,119:136−147. doi: 10.1016/j.eurpolymj.2019.07.021
[9]	WINKLER H, VORWERG W, WETZEL H. Synthesis and properties of fatty acid starch esters[J]. Carbohydr Polym,2013,98(1):208−216. doi: 10.1016/j.carbpol.2013.05.086
[10]	SN A, RV A, AD B, et al. Ultrasound-assisted enzymatic synthesis of xylitol fatty acid esters in solvent-free conditions[J]. Ultrasonics Sonochemistry,2021,7(75):1−8.
[11]	ALLANA C N, GOMES Q C, LÚCIO C M, et al. Enzymatic kinetics of cetyl palmitate synthesis in a solvent-free system[J]. Biochemical Engineering Journal,2018,137:116−124. doi: 10.1016/j.bej.2018.05.021
[12]	HORCHANI H, CHAÂBOUNI M, GARGOURI Y, et al. Solvent-free lipase-catalyzed synthesis of long-chain starch esters using microwave heating: Optimization by response surface methodology[J]. Carbohydrate Polymers,2010,79(2):466−474. doi: 10.1016/j.carbpol.2009.09.003
[13]	王艳, 高鹏, 辛嘉英, 等. 纳米金辅助微波耦合脂肪酶催化阿魏酸淀粉酯的合成[J]. 食品研究与开发,2018,39(24):1−6. [WANG Y, GAO P, XING J Y, et al. Gold nanoparticles assisted microwave coupling lipase catalyzed synthesis of ferulic acid starch ester[J]. Food Research and Development,2018,39(24):1−6. doi: 10.3969/j.issn.1005-6521.2018.24.001
[14]	石佳. 微波辅助脂肪酶催化合成油酸淀粉脂的研究[D]. 哈尔滨: 哈尔滨商业大学, 2014. SHI J. Study on lipase-catalyzed esterification of starch using oleic acid under microwave[D]. Harbin: Harbin University of Commerce, 2014.
[15]	王艳, 赵宁, 王悦, 等. Mb耦合脂肪酶生物传感器差分脉冲伏安法对Cu~(2+)的检测[J/OL]. 食品科学, 1−12 [2022-04-19]. http://kns.cnki.net/kcms/detail/11.2206.TS.20210913.1057.020.html WANG Y, ZHAO N, WANG Y, et al. Detection of Cu²⁺by mb-coupled lipase biosensor with differential pulse voltammetry[J/OL]. Food Science: 1−12 [2022-04-19]. http://kns.cnki.net/kcms/detail/11.2206.TS.20210913.1057.020.html
[16]	王艳. 中长链脂肪酸淀粉酯的酶法合成及其性质研究[D]. 哈尔滨: 哈尔滨商业大学, 2013. WANG Y. Study on enzymatic synthesis and properties of middle long chain fatty acid starch ester[D]. Harbin: Harbin University of Commerce, 2013.
[17]	王艳, 辛嘉英, 石佳, 等. 微波辅助酶促月桂酸淀粉酯的合成[J]. 分子催化,2014,28(1):67−74. [WANG Y, XIN J Y, SHI J, et al. Lipase-catalyzed esterification of starch using lauric acid under microwave[J]. Journal of Molecular Catalysis (China),2014,28(1):67−74. doi: 10.16084/j.cnki.issn1001-3555.2014.01.010
[18]	CABELLO G, DAVOGLIO G A, LUIS G. The role of small nanoparticles on the formation of hot spots under microwave-assisted hydrothermal heating[J]. Inorganic Chemistry: A Research Journal that Includes Bioinorganic, Catalytic, Organometallic, Solid-State, and Synthetic Chemistry and Reaction Dynamics,2018,57(12):7252−7258.
[19]	张宏迪. 纳米金杂化脂肪酶催化拆分反应研究[D]. 哈尔滨: 哈尔滨商业大学, 2020. ZHANG H D. Study on the lipase-gold nanoparticles hybridase-catalyzed resolution reaction[D]. Harbin: Harbin University of Commerce, 2020.
[20]	王致禹, 陈晓倩, 孟庆凤, 等. 有机溶剂和混合油脂对脂肪酶活性恢复的影响[J]. 中国食品学报,2021,21(2):55−62. [WANG Z Y, CHEN X Q, MENG Q F, et al. Effect of organic solvents and mixed oils on the recovery of lipase avity[J]. Journal of Chinese Institute of Food Science and Technology,2021,21(2):55−62. doi: 10.16429/j.1009-7848.2021.02.007
[21]	敖敦格日乐, 杨体强, 包斯琴高娃, 等. 电场对脂肪酶二级结构及其活性的影响[J]. 食品与生物技术学报,2015,34(12):1256−1261. [AO D G R L, YANG T Q, BAO S Q G W, et al. Study on the effect of electric field on the secondary structure and activity of lipase[J]. Journal of Food Science and Biotechnology,2015,34(12):1256−1261. doi: 10.3969/j.issn.1673-1689.2015.12.004
[22]	UPPENBERG J, HANSEN M T, PATKAR S, et al. The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica[J]. Structure,1994,2(4):293−308. doi: 10.1016/S0969-2126(00)00031-9
[23]	LUKASIEWICZ M, KOWALSKI S. Low power microwave-assisted enzymatic esterification of starch[J]. Starch Stä rke,2011,64(3):188−197.
[24]	REJASSE B, BESSONEN T, LEGOY M D, et al. Influence of microwave radiation on free Candida antarctica lipase B activity and stability[J]. Organic & Biomolecular Chemistry,2006,4(19):3703−3707.
[25]	SRI KAJA B, LUMOR S, BESONG S, et al. Investigating enzyme activity of immobilizedCandida rugosa lipase[J]. Journal of Food Quality,2018,4(19):1−9.
[26]	孙纯锐. 硬脂酸淀粉酯的制备及其性质研究[D]. 济南: 齐鲁工业大学, 2016. SUN C Y. The study of preparation and roperties of starch stearate[D]. Jinan: Qilu University of Technology, 2016.
[27]	高鹏. 好食脉孢菌发酵麸皮制备游离阿魏酸及其改性研究[D]. 哈尔滨: 哈尔滨商业大学, 2020. GAO P. Study on the preparation and modification of free ferulic acid from fermentation bran of neurospora fastidious[D]. Harbin: Harbin University of Commerce, 2020.
[28]	金子. 毕赤酵母细胞展示的CALB脂肪酶的表征及非水相催化特性研究[D]. 广州: 华南理工大学, 2013. JIN Z. Characterization and catalytic properties of Candida antarctica lipase B- displaying Pichia pastoris cells in non-aqueous phase[D]. Guangzhou: South China University of Technology, 2013.
[29]	KAPUSNIAK J, SIEMION P. Thermal reactions of starch with long-chain unsaturated fatty acids. Part 2. linoleic acid[J]. Journal of Food Engineering,2007,78(1):323−332. doi: 10.1016/j.jfoodeng.2005.09.028