Antioxidant and Bacteriostatic Activities of Essential Oil from <i>Ligustrum robustum</i> (Rxob.) Blume

MA Qiuyue; XU Qianda; CHEN Nan; GAO Haoxiang; ZENG Weicai

doi:10.13386/j.issn1002-0306.2022020160

Volume 43 Issue 20

Oct. 2022

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Science and Technology of Food Industry > 2022 > 43(20): 173-181. > DOI: 10.13386/j.issn1002-0306.2022020160

MA Qiuyue, XU Qianda, CHEN Nan, et al. Antioxidant and Bacteriostatic Activities of Essential Oil from Ligustrum robustum (Rxob.) Blume[J]. Science and Technology of Food Industry, 2022, 43(20): 173−181. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022020160.

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Antioxidant and Bacteriostatic Activities of Essential Oil from Ligustrum robustum (Rxob.) Blume

MA Qiuyue^1,,
XU Qianda¹,
CHEN Nan¹,
GAO Haoxiang^{1, 2},
ZENG Weicai^{1, 2, ,}

1.
Department of Food Engineering, Sichuan University, Chengdu 610065, China
2.
The Key Laboratory of Food Science and Technology of Sichuan Province of Education, Sichuan University, Chengdu 610065, China

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Received Date: February 20, 2022
Available Online: August 03, 2022

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Abstract

Abstract

Antioxidant and bacteriostatic effects of the essential oil from Ligustrum robustum (Rxob.) Blume (EOL) were investigated. L. robustum was used as the crude material to prepare EOL by steam distillation method. The volatile components of EOL were identified by gas chromatography mass spectrometry (GC-MS). Then, its antioxidant activity was analyzed by some chemical methods in vitro, and its bacteriostatic activity was evaluated by using bacteria, fungi and yeast. Furthermore, peanut oil and orange juice were selected as food models to determine its antioxidant and bacteriostatic effects during storage period. Results showed that 22 of volatile components were identified from EOL, such as 4-terpined, γ-terpinene, and 4-carene, etc.. In addition, EOL could scavenge ABTS⁺ free radical (EC₅₀: 395.9 μg/mL), DPPH free radical (EC₅₀: 165.45 μg/mL), inhibit lipid peroxidation (IC₅₀: 420.1 μg/mL) and showed strong reduce power. Meanwhile, EOL could effectively slow down the increasing of peroxide value of peanut oil, and inhibit the oxidative deterioration of oil during storage. Moreover, EOL showed the favorable broad-spectrum bacteriostatic activities (MIC: 0.31~5.0 μg/mL, MBC: 1.25~20.0 μg/mL), and it inhibited the growth of all testing microorganisms during logarithmic growth period effectively. Specifically, the inhibition of bacteria was obvious, especially for Escherichia coli (the inhibition zone was 29.85 mm). EOL had remarkable abilities to restrain the spoilage and prolong the shelf life of orange juice during storage. All results suggested that the essential oil from L. robustum showed the high value and potential to be developed and utilized in food industry.
- Ligustrum robustum (Rxob.) Blume,
- essential oil,
- antioxidant activity,
- bacteriostatic,
- food storage

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[1]	吕雯雯, 王文庆, 吴华, 等. 脂溶性迷迭香抗氧化剂对油脂的抗氧化作用和生鲜猪肉的保鲜效果[J]. 中国油脂,2021,46(3):142−148. [LÜ W W, WANG W Q, WU H, et al. Effects of fat-soluble rosemary antioxidant on the antioxidant effect of edible oil and preservation of fresh pork[J]. China Oils and Fats,2021,46(3):142−148.
[2]	YU C Y, ZHANG J F, WANG T. Star anise essential oil: Chemical compounds, antifungal and antioxidant activities: A review[J]. Journal of Essential Oil Research, 2021, 33(1): 1−22.
[3]	解久莹, 张翔宇, 吴永强, 等. 煎炸油使用安全及有害成分控制研究进展[J]. 食品工业科技,2019,40(15):333−338. [XIE J Y, ZHANG X Y, WU Y Q, et al. Research progress of safety and harmful component control in frying oil[J]. Science and Technology of Food Industry,2019,40(15):333−338. doi: 10.13386/j.issn1002-0306.2019.15.055
[4]	崔强, 赵治国, 李菁雯, 等. 微生物检验技术研究进展[J]. 食品研究与开发,2018,39(21):211−215. [CUI Q, ZHAO Z G, LI J W, et al. Research progress of microbiological inspection technology[J]. Food Research and Development,2018,39(21):211−215. doi: 10.3969/j.issn.1005-6521.2018.21.035
[5]	尹国利, 赵露, 邹成梅, 等. 超声波辅助提取苦丁茶多酚及其抗氧化与降糖活性研究[J]. 食品研究与开发, 2020, 41(17): 48−55 YIN G L, ZHAO L, ZOU C M, et al. Ultrasound-assisted extraction of polyphenols from llex latifolia Thunb. and its antioxidant and hypoglycemic activities[J]. Food Research and Development 2020, 41(17): 48−55.
[6]	梁恒宇, 高浩祥, 何强, 等. 粗壮女贞苦丁茶提取物对油脂氧化的抑制作用[J]. 食品安全质量检测学报,2021,12(15):6139−6145. [LIANG H Y, GAO H X, HE Q, et al. Inhibition effect of Ligustrum robustum (Rxob.) Blume extract on the oxidation of oil[J]. Food Safety and Quality Detection Technology,2021,12(15):6139−6145.
[7]	YU Z L, ZENG W C. Antioxidant, antibrowning, and cytoprotective activities of Ligustrum robustum (Rxob.)Blume extract[J]. Journal of Food Science,2013,78(9):234−240.
[8]	CORNELIA V, MIHAELA B. Progresses in food packaging, food quality, and safety—controlled-release antioxidant and/or antimicrobial packaging[J]. Molecules,2021,26(5):1263. doi: 10.3390/molecules26051263
[9]	孟雨东, 董颖, 陈鑫沛, 等. 怀菊花精油提取工艺优化[J]. 食品工业科技,2020,41(24):157−161. [MENG Y D, DONG Y, CHEN X P, et al. Optimization of extraction conditions of essential oil from Huai Chrysanthemum[J]. Science and Technology of Food Industry,2020,41(24):157−161.
[10]	曾维才, 石碧. 天然产物抗氧化活性的常见评价方法[J]. 化工进展,2013,32(6):1205−1213. [ZENG W C, SHI B. Common methods of antioxidant activity evaluation for natural products: A review[J]. Chemical Industry and Engineering Progress,2013,32(6):1205−1213.
[11]	高永生, 金斐, 朱丽云, 等. 植物精油及其活性成分的抗菌机理[J]. 中国食品学报,2022,22(1):376−388. [GAO Y S, JIN F, ZHU L Y, et al. Antimicrobial mechanism of plant essential oils and its active ingredients[J]. Journal of Chinese Institute of Food Science and Technology,2022,22(1):376−388.
[12]	黄晓英, 李启明, 吴华星, 等. 传统发酵食品中具有抑菌活性乳酸菌筛选及其代谢产物稳定性分析[J]. 现代食品科技,2021,37(11):68−78. [HUANG X Y, LI Q M, WU H X, et al. Screening of traditional fermented food products for lactic acid bacteria with antibacterial activity and stability analysis of their metabolites[J]. Modern Food Science and Technology,2021,37(11):68−78.
[13]	金虹, 李海帅, 帖金凤, 等. 应用微量稀释法测定消毒剂最小抑菌浓度方法的建立[J]. 中国消毒学杂志,2018,35(11):801−804. [JIN H, LI H S, TIE J F, et al. Establishment of a broth microdilution MIC testing method for disinfectants[J]. Chinese Journal of Disinfection,2018,35(11):801−804. doi: 10.11726/j.issn.1001-7658.2018.11.001
[14]	阚玉红, 谢笔钧, 孙智达. 胭脂红番石榴叶黄酮提取物的抑菌活性及其机理[J]. 中国调味品,2021,46(12):159−166. [KAN Y H, XIE B J, SUN Z D. Antibacterial activity and mechanism of flavonoids extracted from leaves of Psidium guajava Linn doi: 10.3969/j.issn.1000-9973.2021.12.030 J]. China Condiment,2021,46(12):159−166. doi: 10.3969/j.issn.1000-9973.2021.12.030
[15]	王娟, 杜静怡, 贾雪颖, 等. 花椒精油及其水提物的香气活性成分分析[J]. 食品工业科技,2021,42(20):229−241. [WANG J, DU J Y, JIA X Y, et al. Characterization of the aroma-active compounds in essential oil and water extract of Zanthoxylum bungeanum pericarp[J]. Science and Technology of Food Industry,2021,42(20):229−241.
[16]	甘芝霖, 倪元颖. γ-萜品烯的体内外抗氧化性研究[J]. 食品工业科技,2019,40(6):100−105. [GAN Z L, NI Y Y. Antioxidant capacity of γ-Terpinene in vitro andin vivo[J]. Science and Technology of Food Industry,2019,40(6):100−105. doi: 10.13386/j.issn1002-0306.2019.06.017
[17]	YU Z L, ZHANG Z, ZENG W C. Investigation of antibrowning activity of pine needle (Cedrus deodara) extract with fresh-cut apple slice model and identification of the primary active components[J]. European Food Research and Technology,2014,239(4):669−678. doi: 10.1007/s00217-014-2263-3
[18]	陈潘, 席斌, 高雅琴, 等. 4种精油组成成分及总抗氧化活性分析研究[J]. 中国调味品,2020,45(11):60−66. [CHEN P, XI B, GAO Y Q, et al. Analysis of composition and total antioxidant activity of four essential oils[J]. China Condiment,2020,45(11):60−66. doi: 10.3969/j.issn.1000-9973.2020.11.013
[19]	CHEN X, LIANG L, HAN C. Borate suppresses the scavenging activity of gallic acid and plant polyphenol extracts on DPPH radical: A potential interference to DPPH assay[J]. LWT-Food Science and Technology,2020,131(9):1−7.
[20]	王晓宇, 杜国荣, 李华. 抗氧化能力的体外测定方法研究进展[J]. 食品与生物技术学报,2012,31(3):247−252. [WANG X Y, DU G R, LI H. Research progress of plant-derived antimicrobial agents[J]. Journal of Food Science and Biotechnology,2012,31(3):247−252. doi: 10.3969/j.issn.1673-1689.2012.03.004
[21]	SENANAYAKE S N. Green tea extract: Chemistry, antioxidant properties and food applications: A review[J]. Journal of Functional Foods,2013,5(4):1529−1541. doi: 10.1016/j.jff.2013.08.011
[22]	高浩祥, 陈南, 徐乾达, 等. 油炸过程中茶多酚对油脂品质的影响[J]. 食品科学,2021,42(4):1−7. [GAO H X, CHEN N, XU Q D, et al. Effect of tea polyphenols on the quality and storage stability of potato chips during frying[J]. Food Chemistry,2021,42(4):1−7. doi: 10.7506/spkx1002-6630-20191010-075
[23]	曹君, 李红艳, 邓泽元. 植物油氧化稳定性的研究进展[J]. 食品工业科技,2013,34(7):378−382. [CAO J, LI H Y, DENG Z Y. Research progress in oxidative stability of vegetable oil[J]. Science and Technology of Food Industry,2013,34(7):378−382.
[24]	WANG Z M, HE Z F, MARA A, et al. Effects of malondialdehyde as a byproduct of lipid oxidation on protein oxidation in rabbit meat[J]. Food Chemistry,2019,288:405−412. doi: 10.1016/j.foodchem.2019.02.126
[25]	王赛楠, 郭立净, 智文莉, 等. 食用油、油脂及其制品的质量安全风险分析与监管对策研究[J]. 中国油脂,2020,45(9):38−42. [WANG S N, GUO L J, ZHI W L, et al. Risk analysis and supervision countermeasure on quality safety of oils, oils and fats and their products[J]. China Oils and Fats,2020,45(9):38−42.
[26]	CHEMAT F, GRONDIN I, COSTES P, et al. High power ultrasound effects on lipid oxidation of refined sunflower oil[J]. Ultrasonics Sonochemistry,2004,11(5):281−285. doi: 10.1016/j.ultsonch.2003.07.004
[27]	YIN J, ZHANG W J, RICHARDS M P. Attributes of lipid oxidation due to bovine myoglobin, hemoglobin and hemolysate[J]. Food Chemistry,2017,234:335−341.
[28]	朱丹实, 吴逗逗, 沈雨思, 等. 多菌种混合发酵果蔬汁的研究进展[J]. 中国食品学报,2021,21(10):343−352. [ZHU D S, WU D D, SHEN Y S, et al. Research progress on mixed fermentation of multiple strains for fruits and vegetables juice[J]. Journal of Chinese Institute of Food Science and Technology,2021,21(10):343−352.
[29]	YUE H Y, HAN Y Y, YIN B R, et al. Comparison of the antipathogenic effect toward Staphylococcus aureus of N-linked and free oligosaccharides derived from human, bovine, and goat milk[J]. Journal of Food Science,2020,85(8):2713−2721.
[30]	陈志迪, 王新宇, 李晴雯, 等. 植物源抑菌剂的研究进展[J]. 食品安全质量检测学报,2021,12(18):7433−7439. [CHEN Z D, WANG X Y, LI Q W, et al. Research progress of plant-derived antimicrobial agents[J]. Food Safety and Quality Detection Technology,2021,12(18):7433−7439.

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