The Antibacterial Mechanism of Ozone Water Combined with Lactobacillus paracasei Z21 on E.coli O157:H7 in Mung Bean Sprouts

MA Xinmin; GU Hengmei; YANG Weixing; ZHAO Yu; KANG Yanping; CHEN Ping; ZHANG Biying

doi:10.13386/j.issn1002-0306.2022020083

Volume 43 Issue 21

Oct. 2022

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Science and Technology of Food Industry > 2022 > 43(21): 137-143. > DOI: 10.13386/j.issn1002-0306.2022020083

MA Xinmin, GU Hengmei, YANG Weixing, et al. The Antibacterial Mechanism of Ozone Water Combined with Lactobacillus paracasei Z21 on E.coli O157:H7 in Mung Bean Sprouts[J]. Science and Technology of Food Industry, 2022, 43(21): 137−143. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022020083.

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The Antibacterial Mechanism of Ozone Water Combined with Lactobacillus paracasei Z21 on E.coli O157:H7 in Mung Bean Sprouts

College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China

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Received Date: February 13, 2022
Available Online: August 27, 2022

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Abstract

Abstract

To explore the bactericidal effect, cell structure and biofilm removal mechanism of ozone water combined with Lactobacillus paracasei Z21 fermented supernatant on E. coli O157:H7 in mung bean sprouts, this experiment combined treatment of artificially contaminated E. coli O157:H7 to select the optimal bactericidal conditions. The bactericidal mechanism of ozone water combined with Z21 supernatant was studied by flow cytometry, scanning electron microscopy, Fourier transform infrared spectroscopy (FT-IR) and raman spectroscopy. The effect of ozone water combined with Z21 fermented supernatant on E. coli O157:H7 biofilm was studied by colony counting and extracellular polymer analysis. The results showed that 1.5 mg/L ozone water combined with 10% (V/V) Z21 fermented supernatant had the best bactericidal effect on E. coli O157:H7, and the total number of bacteria decreased by 2.81 lg CFU/g. Compared with the control group, the combined treatment destroyed polysaccharides, lipid and protein structures in the cell walls and membranes of E. coli O157:H7, increased the permeability of cell membrane, and changed the morphology. The combined treatment presented a strong removal effect on biofilm, and the extracellular polymer content of the biofilm was significantly reduced (P<0.05). This study could provide a theoretical basis for the removal of Escherichia coli biofilm and preservation of agricultural products.
- mung bean sprout,
- ozone water,
- Escherichia coli O157:H7,
- Lactobacillus paracasei,
- antibacterial mechanism

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