Formulation, Characterization and Pharmacokinetic Studies of <i>Pinus koraiensis</i> Nuts Oil Based Coenzyme Q<sub>10</sub> Loaded Nanoemulsion

WANG Zhongjuan; LI Zihan; ZHANG Xiujuan; LIU Zhiting; CHEN Xiaoqiang; ZHANG Ying

doi:10.13386/j.issn1002-0306.2022020080

Volume 43 Issue 21

Oct. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(21): 225-234. > DOI: 10.13386/j.issn1002-0306.2022020080

WANG Zhongjuan, LI Zihan, ZHANG Xiujuan, et al. Formulation, Characterization and Pharmacokinetic Studies of Pinus koraiensis Nuts Oil Based Coenzyme Q₁₀ Loaded Nanoemulsion[J]. Science and Technology of Food Industry, 2022, 43(21): 225−234. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022020080.

Citation:

PDF (4319 KB)

Formulation, Characterization and Pharmacokinetic Studies of Pinus koraiensis Nuts Oil Based Coenzyme Q₁₀ Loaded Nanoemulsion

WANG Zhongjuan^{1, 2,},
LI Zihan^{1, 2},
ZHANG Xiujuan^{1, 2},
LIU Zhiting^{1, 2},
CHEN Xiaoqiang^{1, 2, 3, 4, 5, 6},
ZHANG Ying^{1, 2, 3, 4, 5, ,}

1.
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China
2.
College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China
3.
Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, China
4.
Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-based Active Substances, Harbin 150040, China
5.
National Engineering Laboratory of BioResource EcoUtilization, Northeast Forestry University, Harbin 150040, China
6.
College of Resources and Environment, Tibet Agriculture & Animal Husbandry University, Nyingchi 860000, China

More Information

Received Date: February 13, 2022
Available Online: August 28, 2022

Graphical Abstract

Abstract

Abstract

Coenzyme Q₁₀ nanoemulsion (CoQ₁₀-NE) was improved and made utilizing Pinus koraiensis nuts oil as the oil phase, soybean lecithin as the surfactant, and ethanol as the cosurfactant in this study. The effects of different factors on the mean particle size and polydispersity index (PDI) of CoQ₁₀-NE were studied, and the quality was evaluated by DLS, TEM, FT-IR, stability, in vitro release behavior and in vivo pharmacokinetic experiments in rats.The results showed that when the mass ratio of CoQ₁₀ to mixed surfactant was 3:40, the mass ratio of CoQ₁₀ to Pinus koraiensis nuts oil was 1:4, the homogenizing pressure was 800 bar, and the number of cycles was 6 times, the CoQ₁₀-NE of mean particle size of 150.30±1.43 nm and PDI of 0.234±0.012 was obtained. The morphology and microstructure showed that the CoQ₁₀-NE was round and non-adhesive at the size of 500 nm and 1 µm. FT-IR results showed that CoQ₁₀ was completely encapsulated in nanoemulsion. The stability experiment showed that there was no significant difference in the mean particle size and PDI (P>0.05). CoQ₁₀-NE had good stability. In vitro release experiments showed that the cumulative dissolution rate of CoQ₁₀-NE was 4.7-fold that of the CoQ₁₀ suspension at 120 min. The dissolution rate was significantly improved. According to the results of pharmacokinetic investigations in rats, the maximum plasma concentration C_max of CoQ₁₀-NE was 2.80-fold that of CoQ₁₀ suspension, the drug concentration of CoQ₁₀-NE in rats was greatly enhanced. The area ${\rm{AUC}}_{0{\text -}\infty}$ was 3.25-fold that of the CoQ₁₀ suspension, demonstrating that CoQ₁₀-NE bioavailability and absorption were greatly increased. The CoQ₁₀-NE developed in this study provides a theoretical foundation for investigating the creation of new CoQ₁₀ formulations and their potential application as a functional ingredient in food.
- coenzyme Q₁₀,
- Pinus koraiensis nuts oil,
- nanoemulsion,
- characterization,
- pharmacokinetics

FullText(HTML)

References (35)

References

[1]	RAIZNER A E. Coenzyme Q₁₀[J]. Methodist DeBakey Cardiovascular Journal,2019,15(3):185−191. doi: 10.14797/mdcj-15-3-185
[2]	YANG C X, LIU S, MIAO J K, et al. CoQ₁₀ improves meiotic maturation of pig oocytes through enhancing mitochondrial function and suppressing oxidative stress[J]. Theriogenology,2021,159:77−86. doi: 10.1016/j.theriogenology.2020.10.009
[3]	ELSHAZLY S M, ALSEMEH A E, AHMAD E A A, et al. CoQ₁₀ exerts hepatoprotective effect in fructose-induced fatty liver model in rats[J]. Pharmacological Reports,2020,72(4):922−934. doi: 10.1007/s43440-020-00075-5
[4]	AGAMY D, NAGUIB Y M. CoQ₁₀ ameliorates monosodium glutamate-induced alteration in detrusor activity and responsiveness in rats via anti-inflammatory, anti-oxidant and channel inhibiting mechanisms[J]. BMC Urology,2019,19(1):103. doi: 10.1186/s12894-019-0534-9
[5]	ZOZINA V, COVANTEV S, GOROSHKO O, et al. Coenzyme Q₁₀ in cardiovascular and metabolic diseases: Current state of the problem[J]. Current Cardiology Reviews,2018,14(3):164−174. doi: 10.2174/1573403X14666180416115428
[6]	AASETH J, ALEXANDER J, ALEHAGEN U. Coenzyme Q₁₀ supplementation-in ageing and disease[J]. Mechanisms of Ageing and Development,2021,197:111521. doi: 10.1016/j.mad.2021.111521
[7]	TAFAZOLI A. Coenzyme Q₁₀ in breast cancer care[J]. Future Oncology,2017,13(11):1035−1041. doi: 10.2217/fon-2016-0547
[8]	AF MARÍA, EVA T, GLORIA B C. Clinical syndromes associated with coenzyme Q₁₀ deficiency[J]. Essays in Biochemistry,2018,62(3):377−398. doi: 10.1042/EBC20170107
[9]	PRAVST I, AGUILERA J, RODRIGUEZ A, et al. Comparative bioavailability of different coenzyme Q10 formulations in healthy elderly individuals[J]. Nutrients,2020,12(3):784. doi: 10.3390/nu12030784
[10]	CHEN X, ZHANG Y, WANG Z, et al. In vivo antioxidant activity of Pinus koraiensis nut oil obtained by optimised supercritical carbon dioxide extraction[J]. Natural Product Research,2011,25(19):1807−1816. doi: 10.1080/14786419.2010.483229
[11]	SHPATOV A V, POPOV S A, SALNIKOVA O I, et al. Composition and bioactivity of lipophilic metabolites from needles and twigs of Korean and Siberian Pines (Pinus koraiensis Siebold & Zucc. and Pinus sibirica Du Tour)[J]. Chemistry & Biodiversity, 2017, 14(2):e1600203.
[12]	TAKALA R, RAMJI D P, ANDREWS R, et al. Anti-inflammatory and immunoregulatory effects of pinolenic acid in rheumatoid arthritis[J]. Rheumatology (Oxford),2022,61(3):992−1004. doi: 10.1093/rheumatology/keab467
[13]	ZHANG Y, XIN C, QIU J, et al. Essential oil from Pinus koraiensis pinecones inhibits gastric cancer cells via the HIPPO/YAP signaling pathway[J]. Molecules,2019,24(21):3851. doi: 10.3390/molecules24213851
[14]	SINGH Y, MEHER J G, RAVAL K, et al. Nanoemulsion: Concepts, development and applications in drug delivery[J]. Journal of Controlled Release,2017,252:28−49. doi: 10.1016/j.jconrel.2017.03.008
[15]	PANDEY P, GULATI N, MAKHIJA M, et al. Nanoemulsion: A novel drug delivery approach for enhancement of bioavailability[J]. Recent Patents on Nanotechnology,2020,14(4):276−293. doi: 10.2174/1872210514666200604145755
[16]	张平. 复合抗衰老纳米乳液的制备及其应用评估[D]. 南京: 东南大学, 2017 ZHANG Ping. Preparation and application evaluation of compound antiaging nano emulsion[D]. Nanjing: Southeast University, 2017.
[17]	FAN H, LIU G, HUANG Y, et al. Development of a nanostructured lipid carrier formulation for increasing photo-stability and water solubility of phenylethyl resorcinol[J]. Applied Surface Science,2014,288:193−200. doi: 10.1016/j.apsusc.2013.10.006
[18]	BHALEKAR M R, POKHARKAR V, MADGULKAR A, et al. Preparation and evaluation of miconazole nitrate-loaded solid lipid nanoparticles for topical delivery[J]. AAPS Pharm Sci Tech,2009,10(1):289−296. doi: 10.1208/s12249-009-9199-0
[19]	ZHONG Y, WANG J, WANG Y, et al. Preparation and evaluation of liposome-encapsulated codrug LMX[J]. International Journal of Pharmaceutics,2012,438(1-2):240−248. doi: 10.1016/j.ijpharm.2012.08.051
[20]	周佳, 孙燕燕, 温美强, 等. 布洛芬口服纳米乳的制备及处方优化研究[J]. 沈阳药科大学学报,2020,37(10):865−871. [ZHOU Jia, SUN Yanyan, WEN Meiqiang, et al. Preparation and optimization of ibuprofen loaded oral nanoemulsion[J]. Journal of Shenyang Pharmaceutical University,2020,37(10):865−871. doi: 10.14066/j.cnki.cn21-1349/r.2020.10.001
[21]	KHATTAB A, HASSANIN L, ZAKI N. Self-nanoemulsifying drug delivery system of coenzyme Q₁₀ with improved dissolution, bioavailability, and protective efficiency on liver fibrosis[J]. AAPS Pharm Sci Tech,2017,18(5):1657−1672. doi: 10.1208/s12249-016-0632-x
[22]	KACI M, ARAB-TEHRANY E, DOSTERT G, et al. Efficiency of emulsifier-free emulsions and emulsions containing rapeseed lecithin as delivery systems for vectorization and release of coenzyme Q₁₀: Physico-chemical properties and in vitro evaluation[J]. Colloids and Surfaces B: Biointerfaces,2016,147:142−150. doi: 10.1016/j.colsurfb.2016.07.036
[23]	KOMMURU T R, GURLEY B; KHAN M A, et al. Self-emulsifying drug delivery systems (SEDDS) of coenzyme Q₁₀: Formulation development and bioavailability assessment[J]. International Journal of Pharmaceutics,2001,212(2):233−246. doi: 10.1016/S0378-5173(00)00614-1
[24]	HALLOUARD F, DOLLO G, BRANDHONNEUR N, et al. Preparation and characterization of spironolactone-loaded nanoemulsions for extemporaneous applications[J]. International Journal of Pharmaceutics,2015,478(1):193−201. doi: 10.1016/j.ijpharm.2014.11.046
[25]	杨璇. 辅酶Q₁₀纳米制剂制备、表征及其生物效应研究[D]. 哈尔滨: 东北林业大学, 2018 YANG Xuan. Preparation, characterization and biological effects of coenzyme Q₁₀ nanoemulsion[D]. Harbin: Northeast Forestry University, 2018.
[26]	GARAVAND F, JALAI-JIVAN M, ASSADPOUR E, et al. Encapsulation of phenolic compounds within nano/microemulsion systems: A review[J]. Food Chemistry,2021,364(4):130376.
[27]	WU Y, PETROCHENKO P, MYUNG J H, et al. Distinguish coexistence of nanoemulsion and liposome in propofol by cryogenic transmission electron microscopy (cryo-TEM)[J]. Microscopy & Microanalysis,2017,23(S1):852−853.
[28]	YULIATI L. Curcumin-loaded nanoemulsion for better cellular permeation[J]. Scientia Pharmaceutica,2020,88(4):44. doi: 10.3390/scipharm88040044
[29]	TUBESHA Z, ABUBAKAR Z, ISMAIL M. Characterization and stability evaluation of thymoquinone nanoemulsions prepared by high-pressure homogenization[J]. Journal of Nanomaterials,2013,2013:1−6.
[30]	SABJAN K B, MUNAWAR S M, RAJENDIRAN D, et al. Nanoemulsion as oral drug delivery-A review[J]. Current Drug Research Reviews,2020,12(1):4−15. doi: 10.2174/2589977511666191024173508
[31]	CHUTIA H, MAHANTA C L. Properties of starch nanoparticle obtained by ultrasonication and high pressure homogenization for developing carotenoids-enriched powder and pickering nano-emulsion[J]. Innovative Food Science and Emerging Technologies,2021,74:102822. doi: 10.1016/j.ifset.2021.102822
[32]	KHAN A W, KOTTA S, ANSARI S H, et al. Self-nanoemulsifying drug delivery system (SNEDDS) of the poorly water-soluble grapefruit flavonoid naringenin: Design, characterization, in vitro and in vivo evaluation[J]. Drug Delivery,2015,22(4):552−561. doi: 10.3109/10717544.2013.878003
[33]	陈家铃, 吴航航, 何益飞, 等. 水杨酸纳米乳的制备、体外释放行为及抑菌活性研究[J]. 精细与专用化学品,2022,30(3):16−21. [CHEN Jialing, WU Hanghang, HE Yifei, et al. Preparation, in vitro release behavior and antibacterial activity of salicylic acid nanoemulsion[J]. Fine and Specialty Chemicals,2022,30(3):16−21. doi: 10.19482/j.cn11-3237.2022.03.04
[34]	CHHABRA G, CHUTTANI K, MISHRA A K, et al. Design and development of nanoemulsion drug delivery system of amlodipine besilate for improvement of oral bioavailability[J]. Drug Development and Industrial Pharmacy,2011,37:907−916. doi: 10.3109/03639045.2010.550050
[35]	BELHAJ N, DUPUIS F, ARAB-TEHRANY E, et al. Formulation, characterization and pharmacokinetic studies of coenzyme Q₁₀ PUFA's nanoemulsions[J]. European Journal of Pharmaceutical Sciences,2012,47(2):305−312. doi: 10.1016/j.ejps.2012.06.008

Supplements (0)

Cited By

Cited by

Periodical cited type(6)

1.	郭利健. 茶多酚与表面活性剂的相互作用及其在食品工业中的应用. 安徽化工. 2025(01): 26-29 .
2.	郝吉明. 基于淀粉作用的大米品质提升策略研究. 粮油与饲料科技. 2024(08): 225-227 .
3.	郭硕，刘景圣，郑明珠. 热处理过程中食品组分与淀粉相互作用研究进展. 食品安全质量检测学报. 2023(01): 17-24 .
4.	华玮，熊丽娜，张永鑫，孙鹏，岳龙，付鸿博. 草莓多酚提取工艺优化及抗氧化性分析. 果树资源学报. 2023(06): 17-22 .
5.	李舒玥，吴昊怡，易阳，孙莹，彭凯迪，江雪玉. 多酚强化莲藕营养粉的制备与食用品质. 武汉轻工大学学报. 2023(05): 1-10 .
6.	柳丝菁，孙红男，马梦梅，木泰华. 淀粉-多酚复合物制备及其消化特性研究进展. 食品安全质量检测学报. 2023(22): 152-161 .