Formulation, Characterization and Pharmacokinetic Studies of <i>Pinus koraiensis</i> Nuts Oil Based Coenzyme Q<sub>10</sub> Loaded Nanoemulsion

WANG Zhongjuan; LI Zihan; ZHANG Xiujuan; LIU Zhiting; CHEN Xiaoqiang; ZHANG Ying

doi:10.13386/j.issn1002-0306.2022020080

Volume 43 Issue 21

Oct. 2022

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Science and Technology of Food Industry > 2022 > 43(21): 225-234. > DOI: 10.13386/j.issn1002-0306.2022020080

WANG Zhongjuan, LI Zihan, ZHANG Xiujuan, et al. Formulation, Characterization and Pharmacokinetic Studies of Pinus koraiensis Nuts Oil Based Coenzyme Q₁₀ Loaded Nanoemulsion[J]. Science and Technology of Food Industry, 2022, 43(21): 225−234. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022020080.

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Formulation, Characterization and Pharmacokinetic Studies of Pinus koraiensis Nuts Oil Based Coenzyme Q₁₀ Loaded Nanoemulsion

WANG Zhongjuan^{1, 2,},
LI Zihan^{1, 2},
ZHANG Xiujuan^{1, 2},
LIU Zhiting^{1, 2},
CHEN Xiaoqiang^{1, 2, 3, 4, 5, 6},
ZHANG Ying^{1, 2, 3, 4, 5, ,}

1.
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China
2.
College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China
3.
Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, China
4.
Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-based Active Substances, Harbin 150040, China
5.
National Engineering Laboratory of BioResource EcoUtilization, Northeast Forestry University, Harbin 150040, China
6.
College of Resources and Environment, Tibet Agriculture & Animal Husbandry University, Nyingchi 860000, China

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Received Date: February 13, 2022
Available Online: August 28, 2022

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Abstract

Abstract

Coenzyme Q₁₀ nanoemulsion (CoQ₁₀-NE) was improved and made utilizing Pinus koraiensis nuts oil as the oil phase, soybean lecithin as the surfactant, and ethanol as the cosurfactant in this study. The effects of different factors on the mean particle size and polydispersity index (PDI) of CoQ₁₀-NE were studied, and the quality was evaluated by DLS, TEM, FT-IR, stability, in vitro release behavior and in vivo pharmacokinetic experiments in rats.The results showed that when the mass ratio of CoQ₁₀ to mixed surfactant was 3:40, the mass ratio of CoQ₁₀ to Pinus koraiensis nuts oil was 1:4, the homogenizing pressure was 800 bar, and the number of cycles was 6 times, the CoQ₁₀-NE of mean particle size of 150.30±1.43 nm and PDI of 0.234±0.012 was obtained. The morphology and microstructure showed that the CoQ₁₀-NE was round and non-adhesive at the size of 500 nm and 1 µm. FT-IR results showed that CoQ₁₀ was completely encapsulated in nanoemulsion. The stability experiment showed that there was no significant difference in the mean particle size and PDI (P>0.05). CoQ₁₀-NE had good stability. In vitro release experiments showed that the cumulative dissolution rate of CoQ₁₀-NE was 4.7-fold that of the CoQ₁₀ suspension at 120 min. The dissolution rate was significantly improved. According to the results of pharmacokinetic investigations in rats, the maximum plasma concentration C_max of CoQ₁₀-NE was 2.80-fold that of CoQ₁₀ suspension, the drug concentration of CoQ₁₀-NE in rats was greatly enhanced. The area ${\rm{AUC}}_{0{\text -}\infty}$ was 3.25-fold that of the CoQ₁₀ suspension, demonstrating that CoQ₁₀-NE bioavailability and absorption were greatly increased. The CoQ₁₀-NE developed in this study provides a theoretical foundation for investigating the creation of new CoQ₁₀ formulations and their potential application as a functional ingredient in food.
- coenzyme Q₁₀,
- Pinus koraiensis nuts oil,
- nanoemulsion,
- characterization,
- pharmacokinetics

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References (35)

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