Effects of Ultrasound Treatment on Physicochemical Properties and Antioxidant Ability of Kidney Bean Protein

ZHOU Xinyu; ZUO Zhaohang; WANG Ying; YAO Yang; SUN Wei; ZHANG Naidan; PANG Weiqiao

doi:10.13386/j.issn1002-0306.2022010203

Volume 43 Issue 20

Oct. 2022

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Science and Technology of Food Industry > 2022 > 43(20): 107-112. > DOI: 10.13386/j.issn1002-0306.2022010203

ZHOU Xinyu, ZUO Zhaohang, WANG Ying, et al. Effects of Ultrasound Treatment on Physicochemical Properties and Antioxidant Ability of Kidney Bean Protein[J]. Science and Technology of Food Industry, 2022, 43(20): 107−112. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022010203.

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Effects of Ultrasound Treatment on Physicochemical Properties and Antioxidant Ability of Kidney Bean Protein

ZHOU Xinyu^1,,
ZUO Zhaohang¹,
WANG Ying^{1, 2, 3, 4, ,},
YAO Yang^5, ,,
SUN Wei¹,
ZHANG Naidan¹,
PANG Weiqiao¹

1.
College of Food, Heilongjiang Bayi Agricultural University, Daqing 163319, China
2.
National Coarse Cereals Engineering Research Center, Daqing 163319, China
3.
Engineering Research Center of Processing and Utilization of Grain By-products, Ministry of Education, Daqing 163319, China
4.
Key Laboratory of Agro-Products Processing and Quality Safety of Heilongjiang Province, Daqing 163319, China
5.
Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China)

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Received Date: January 23, 2022
Available Online: July 26, 2022

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Abstract

Abstract

In this experiment, degreased kidney bean protein was used as raw material. The effects of different ultrasonic conditions on the physicochemical properties and antioxidant capacity of kidney bean protein were analyzed by solubility, emulsification and total antioxidant capacity. The results showed that compared with the untreated group, when the ultrasonic time was 20 min and the power was 400 W, the hydrolysis degree and solubility of kidney bean protein reached the maximum value, which increased from 3.34% to 20.10% and 56.83% to 79.63%, respectively. Foamability and foamability stability of kidney bean protein were significantly enhanced after ultrasonic treatment (P<0.05), when the ultrasonic time was 10 min and the power was 400 W, the maximum value was 162.81%, 72.94%. Compared with the untreated group, when the ultrasonic time was 10 min and the power was 400 W, the emulsification was the strongest, increasing by 2.82 m²/g. When the ultrasonic power remained unchanged, the time was extended to 20 min, the emulsification stability increased by 18.95%. Ultrasonic treatment significantly increased the content of free sulfhydryl group and decreased the content of disulfide bond in kidney bean protein (P<0.05). The total antioxidant capacity (674.63 U/mL) was the best when the ultrasonic time was 30 min and the power was 160 W. The scavenging ability of DPPH free radical and reducing ability of Fe ion reached the maximum value when ultrasonic time was 10 min and ultrasonic power was 400 W, which were increased by 38.96% and 17.84%, respectively. In summary, ultrasound treatment could significantly improve the physical and chemical properties of kidney bean protein and enhance its antioxidant capacity.
- ultrasound,
- kidney bean protein,
- physicochemical properties,
- antioxidant capacity

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References (31)

References

[1]	研究建议: 多吃植物蛋白有助预防糖尿病[J]. 食品工业, 2017, 38(6): 285 Research suggests eating more plant protein may help prevent diabetes[J]. Food Industry, 2017, 38(6): 285.
[2]	刘晓荻. 用植物蛋白代替红肉有益于心脏健康[J]. 基础医学与临床,2019,39(11):1638. [LIU X D. Substituting plant protein for red meat is heart-healthy[J]. Basic Medicine and Clinical Practice,2019,39(11):1638. doi: 10.16352/j.issn.1001-6325.2019.11.030
[3]	徐克聪. 新疆的食用豆类作物[J]. 新疆农业科学,1993,9(1):12−15. [XU K C. Edible legume crops in Xinjiang[J]. Xinjiang Agricultural Sciences,1993,9(1):12−15.
[4]	马文鹏, 任海伟. 芸豆蛋白的提取及其营养价值评价[J]. 食品科技,2013,38(1):75−79. [MA W P, REN H W. Extraction and nutritional value evaluation of kidney bean protein[J]. Food Science and Technology,2013,38(1):75−79. doi: 10.13684/j.cnki.spkj.2013.01.005
[5]	CHARLES U, IDOLO I, JOB A, et al. Kidney bean protein products as potential antioxidative and antihypertensive alternatives for non-pharmacological inhibition of angiotensin-converting enzymes[J]. Scientific African,2021:11.
[6]	SEYED M, TAGHI G, BRENNAN S. The functional modification of legume proteins by ultrasonication: A review[J]. Trends in Food Science & Technology,2020,98(C):107−116.
[7]	JIAN J, OGADIMMA D O, CHIBUIKE C U, et al. Effects of sonication on the in vitro digestibility and structural properties of buckwheat protein isolates[J]. Ultrasonics Sonochemistry,2020,70:105348−105348.
[8]	XIAO S. Effect of high intensity ultrasound on physicochemical and functional properties of kidney bean protein[J]. Cereals and Oils,2021(34):93−96.
[9]	XIONG T, XIONG W, GE M, et al. Effect of high intensity ultrasound on structure and foaming properties of pea protein isolate[J]. Food Research International,2019(109):260−267.
[10]	LI S Y, XUE Y, ZHANG Y Y, et al. Effects of ultrasound and ultrasound assisted alkaline pretreatments on the enzymolysis and structural characteristics of rice protein[J]. Ultrasonics-Sonochemistry,2016,31:20−28. doi: 10.1016/j.ultsonch.2015.11.019
[11]	WU Q Y, ZHANG X F, JIA J Q, et al. Effect of ultrasonic pretreatment on whey protein hydrolysis by alcalase: Thermodynamic parameters, physicochemical properties and bioactivities[J]. Process Biochemistry,2018,67:46−54. doi: 10.1016/j.procbio.2018.02.007
[12]	TOMOTAKE H, SHIMAOKA I, KAYASHITA J, et al. Physicochemical and functional properties of buckwheat protein product[J]. Journal of Agricultural and Food Chemistry,2002,50(7):2125−2129. doi: 10.1021/jf011248q
[13]	JAMBRAK A R, TIMOTHY J M, VEANA L, et al. Effect of ultrasound treatment on particle size and molecular weight of whey proteins[J]. Journal of Food Engineering,2014,121:15−23. doi: 10.1016/j.jfoodeng.2013.08.012
[14]	JAMBRAK A R, LELAS V, MASON T J, et al. Physical properties of ultrasound treated soy proteins[J]. Journal of Food Engineering,2009,93(4):386−393. doi: 10.1016/j.jfoodeng.2009.02.001
[15]	JIANG J, CHEN J, XIONG Y L. Structural and emulsifying properties of soy protein isolate subjected to acid and alkaline pH-shifting processes[J]. Journal of Agricultural and Food Chemistry,2009,57(16):7576−7583. doi: 10.1021/jf901585n
[16]	WU W, HETTIARACHCHY N S, QI M. Hydrophobicity, solubility and emulsifying properties of soy protein peptides prepared by papain modification and ultrafiltration[J]. Journal of the American Oli Chemists Society,1998,75(7):845−850. doi: 10.1007/s11746-998-0235-0
[17]	张雪春, 廖靖怡, 谢颖欣, 等. 藜麦提取物的抗氧化活性及藜麦红豆复合饮料的研制[J]. 生物加工过程,2018,16(3):91−101. [ZHANG X Y, MIAO J Y, XIE Y X, et al. Antioxidant activity of quinoa extract and preparation of quinoa red bean compound beverage[J]. Chinese Journal of Bioprocess Engineering,2018,16(3):91−101. doi: 10.3969/j.issn.1672-3678.2018.03.013
[18]	杜双奎, 于修烛, 李志西. 超声波预处理对鹰嘴豆蛋白制备抗氧化肽的影响[J]. 中国食品学报,2013,13(1):80−88. [DU S K, YU X Z, LI Z X. Effects of ultrasonic pretreatment on the preparation of antioxidant peptides from chickpea protein[J]. Chinese Journal of Food Science,2013,13(1):80−88.
[19]	苏春燕, 朱科学, 周惠明. 超声波频率对谷朊粉酶解产物抗氧化性的影响[J]. 中国粮油学报,2010,25(9):1−4. [SU C Y, ZHU K X, ZHOU H M. Effect of ultrasonic frequency on antioxidant activity of gluten hydrolysates[J]. Chinese Journal of Cereals and Oils,2010,25(9):1−4.
[20]	DU M, XIE J, GONG B, et al. Extraction, physicochemical characteristics and functional properties of mung bean protein[J]. Food Hydrocolloids,2018,76:131−140. doi: 10.1016/j.foodhyd.2017.01.003
[21]	刁小琴, 关海宁, 乔秀丽, 等. 超声波处理对猪肉肌原纤维蛋白理化及乳化特性的影响[J]. 食品与机械,2019,35(4):26−30, 36. [DIAO X Q, GUAN H N, QIAO X L, et al. Effects of ultrasonic treatment on physicochemical and emulsifying properties of pork myofibrin[J]. Food and Machinery,2019,35(4):26−30, 36.
[22]	ZHONG Z F, XIONG L. Thermosonication-induced structural changes and solution properties of mung bean protein[J]. Ultrasonics Sonochemistry,2019,62(C):104908.
[23]	ARZENI C, MARTINEZ K, ZEMA P, et al. Comparative study of high intensity ultrasound effects on food proteins functionality[J]. Journal of Food Engineering,2012,108(3):463−472. doi: 10.1016/j.jfoodeng.2011.08.018
[24]	HU H, FAN X, ZHOU Z, et al. Acid-induced gelation behavior of soybean protein isolate with high intensity ultrasonic pre-treatments[J]. Ultrasonics Sonochemistry,2013,20(1):187−195. doi: 10.1016/j.ultsonch.2012.07.011
[25]	LEE H, YILDIZ G, JIANG S, et al. Soy protein nano-aggregates with improved functional properties prepared by sequential pH treatment and ultrasonication[J]. Food Hydrocolloids,2016,55:200−209. doi: 10.1016/j.foodhyd.2015.11.022
[26]	王营娟. 超声对鹰嘴豆分离蛋白理化和功能特性的影响[D]. 郑州: 郑州轻工业大学, 2021 WANG Y J. Effects of ultrasound on physicochemical and functional properties of chickpea protein isolate[D]. Zhengzhou: Zhengzhou University of Light Industry, 2021.
[27]	李杨, 田甜, 刘军, 等. 超声对大豆分离蛋白结构及乳化特性影响研究[J]. 食品工业,2019,40(1):184−188. [LI Y, TIAN T, LIU J, et al. Effects of ultrasound on structure and emulsification properties of soybean protein isolate[J]. Food Industry,2019,40(1):184−188.
[28]	仲志峰. 热超声处理对绿豆分离蛋白结构和界面活性及乳化功能的影响[D]. 无锡: 江南大学, 2020 ZHONG Z F. Effects of thermoultrasonic treatment on structure, interfacial activity and emulsification function of mung bean protein isolate[D]. Wuxi: Jiangnan University, 2020.
[29]	望运滔, 王营娟, 田金风, 等. 高强度超声对鹰嘴豆分离蛋白结构和功能特性的影响[J]. 食品与机械,2020,36(8):9−14,71. [WANG Y T, WANG Y J, TIAN J F, et al. Effects of high intensity ultrasound on structural and functional properties of chickpea protein isolate[J]. Food and Machinery,2020,36(8):9−14,71.
[30]	PENTA-RAMOS E A, XIONG Y L. Antioxidative activity of whey protein hydrolysates in a liposomal system[J]. Journal of Dairy Science,2001,84(12):2577−2583. doi: 10.3168/jds.S0022-0302(01)74711-X
[31]	卢阳, 王凤翼, 孔繁东. 大豆蛋白酶水解物抗氧化性的研究[J]. 大连轻工业学院学报,2001,20(4):257−259. [LU Y, WANG F Y, KONG F D. Study on antioxidant activity of soybean protease hydrolysate[J]. Journal of Dalian Institute of Light Industry,2001,20(4):257−259.