ZHAO Qiongyu, HU Jian, LI Caiyan, et al. Ultrasound-Assisted Decalcification Process Optimizationof Carapace of Chinese Soft-shelled Turtle and Biochemical Characterization of Collagen[J]. Science and Technology of Food Industry, 2022, 43(22): 39−51. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022010104.
Citation: ZHAO Qiongyu, HU Jian, LI Caiyan, et al. Ultrasound-Assisted Decalcification Process Optimizationof Carapace of Chinese Soft-shelled Turtle and Biochemical Characterization of Collagen[J]. Science and Technology of Food Industry, 2022, 43(22): 39−51. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022010104.

Ultrasound-Assisted Decalcification Process Optimizationof Carapace of Chinese Soft-shelled Turtle and Biochemical Characterization of Collagen

More Information
  • Received Date: January 13, 2022
  • Available Online: September 06, 2022
  • In order to determine the technological conditions for decalcification of the carapace from the Chinese soft-shelled turtle(Pelodiscus sinensis), the decalcification conditions were optimized under ultrasonic assisted treatment with hydrochloric acid as a decalcifying agent. The decalcification rate and collagen migration rate were employed as evaluation indicators to investigate the effects of six factors including hydrochloric acid concentration, material-to-liquid ratio, decalcified time, decalcified temperature, working time of ultrasonic gap and ultrasonic power. In basis of optimized single factors, the Box-Behnken design in the response surface was used to optimize the process conditions of turtle carapace decalcification and establish a mathematical model. The effects of ultrasound-assisted decalcification process on the structural properties of collagen were investigated through biochemical character such as Ultraviolet spectrum scanning, SDS-PAGE spectrum, Fourier infrared scanning, amino acid analysis and Circular dichroism spectrum. The results showed that the best process conditions for ultrasonic-assisted decalcification of the carapace were: 0.8 mol/L of hydrochloric acid concentration, 1:40 (g/mL) of material-to-liquid ratio, 60 min of decalcified time, 145 W of ultrasound and 30 s/30 s of ultrasonic gap. Under these conditions, the decalcification rate was 64.97%±1.03%, and the collagen migration rate was 3.22%±0.84%. The results showed that the subunit composition, triple helix structure and secondary structure of carapace collagen remained unchanged. Therefore, ultrasound-assisted hydrochloric acid exhibited a good effect on the decalcification of the carapace of the Chinese soft-shelled turtle, which would provide a basis for further study concerning extraction of collagen from the carapace of the Chinese soft-shelled turtle.
  • [1]
    武文霞, 于同慧, 朱祎, 等. 骨胶原蛋白的制备及其在食品中应用的研究进展[J]. 食品工业科技,2022,42(13):445−454. [WU W X, YU T H, ZHU Y, et al. Research progress on preparation and application in food industry of bone collagen[J]. Science and Technology of Food Industry,2022,42(13):445−454. doi: 10.13386/j.issn1002-0306.2021070289
    [2]
    PAULY D, ZELLER D. Comments on FAOs state of world fisheries and aquaculture (SOFIA 2016)[J]. Marine Policy,2017,77:176−181. doi: 10.1016/j.marpol.2017.01.006
    [3]
    ZHAO W H, CHI C F, ZHAO Y Q, et al. Preparation, physicochemical and antioxidant properties of acid-and pepsin-soluble collagens from the swim bladders of miiuy croaker (Miichthys miiuy)[J]. Marine drugs,2018,16(5):161. doi: 10.3390/md16050161
    [4]
    何云, 包建强. 关于鱼骨成分分析的研究进展[J]. 上海农业科技,2017,46(4):28−31. [HE Y, BAO J Q. Research Progress on composition analysis of fish bones[J]. Shanghai Agricultural Science and technology,2017,46(4):28−31. doi: 10.3969/j.issn.1001-0106.2017.04.012
    [5]
    刘俐, 何清湖, 唐宇, 等. 龟甲的现代研究进展[J]. 湖南中医杂志,2020,36(7):181−183. [LIU L, HE Q H, TANG Y, et al. Modern research progress of tortoise shell[J]. Hunan Journal of Tradttional Chinese Medicine,2020,36(7):181−183. doi: 10.16808/j.cnki.issn1003-7705.2020.07.068
    [6]
    陈弘培, 赵慧, 董诗婷, 等. 鳄鱼综合加工与高值化利用研究进展[J]. 农产品加工 (下),2017(10):62−67. [CHEN H P, ZHAO H, DONG S T, et al. Research progress on comprehensive processing and high value utilization of crocodiles[J]. Farm Products Processing,2017(10):62−67.
    [7]
    李晓, 王颖, 李红艳, 等. 多棘海盘车体壁脱钙工艺的研究[J]. 食品工业科技,2016,37(10):307−310. [LI X, WANG Y, LI H Y, et al. Study on optimization and changes of bodywall of Asterias amurensis in the process of decalcification[J]. Science and Technology of Food Industry,2016,37(10):307−310. doi: 10.13386/j.issn1002-0306.2016.10.054
    [8]
    熊磊, 陈正平, 李敏, 等. 鳙鱼鱼骨脱钙工艺优化及其模型[J]. 南昌大学学报 ( 工科版 ) ,2018,40(2):131−136. [XIONG L, CHEN Z P, LI M, et al. Process optimization and model for decalcification of bighead carp fish bone[J]. Journal of Nanchang University (Engineering & Technology),2018,40(2):131−136. doi: 10.13764/j.cnki.ncdg.2018.02.005
    [9]
    李文凤, 王标诗, 余石坚, 等. 响应面法优化黄花鱼鱼鳞脱钙工艺[J]. 食品工业科技,2021,42(4):155−160. [LI W F, WANG B S, YU S J, et al. Optimization of decalcification process of yellow croaker scales by response surface methodology[J]. Science and Technology of Food Industry,2021,42(4):155−160. doi: 10.13386/j.issn1002-0306.2019100209
    [10]
    WANG Y, REGENSTEIN J M. Effect of EDTA, HCl, and citric acid on Ca salt removal from Asian (silver) carp scales prior to gelatin extraction[J]. Journal of Food Science,2009,74(6):C426−C431. doi: 10.1111/j.1750-3841.2009.01202.x
    [11]
    吴锁连, 康怀彬, 李冬姣. 鱼鳞有效成分提取技术的研究进展[J]. 安徽农业科学,2017,45(27):110−112. [WU S L, KANG H B, LI D J. Research progress on extraction technology of effective components in fish scale[J]. Journal of Anhui Agri,2017,45(27):110−112. doi: 10.3969/j.issn.0517-6611.2017.27.034
    [12]
    ZOU Y, XU P, LI P, et al. Effect of ultrasound pre-treatment on the characterization and properties of collagen extracted from soft-shelled turtle (Pelodiscus sinensis)[J]. LWT-Food Science and Technology,2017,82:72−81. doi: 10.1016/j.lwt.2017.04.024
    [13]
    李根, 任国艳, 李倩, 等. 超声辅助提取鲵皮胶原蛋白工艺优化及特性分析[J]. 食品工业科技,2021,42(22):160−168. [LI G, REN G Y, LI Q, et al. Optimization of ultrasound-assisted extraction and structural characteristics analysis of collagen from Chinese giant salamander (Andrias davidianus) skin[J]. Science and Technology of Food Industry,2021,42(22):160−168.
    [14]
    李明华, 孟秀梅. 超声波辅助柠檬酸法龙虾壳脱钙工艺研究[J]. 食品工业,2017(5):8−10. [LI M H, MENG X M. Process optimization of calcium removal from shrimp shell by ultrasonic-assisted citric acid[J]. Food Industry,2017(5):8−10.
    [15]
    王梅英, 陈慧斌, 吴云辉. 超声波辅助酸法脱除鱼鳞钙工艺研究[J]. 宁德师范学院学报:自然科学版,2013,25(4):383−385. [WANG M Y, CHEN H B, WU Y H. Study on removal of calcium from fish scales by ultrasonic assisted acid method[J]. Journal of Ningde Normal University (Natural Science),2013,25(4):383−385.
    [16]
    SONG K M, JUNG S K, KIM Y H, et al. Development of industrial ultrasound system for mass production of collagen and biochemical characteristics of extracted collagen[J]. Food and Bioproducts Processing,2018,110:96−103. doi: 10.1016/j.fbp.2018.04.001
    [17]
    邹烨, 蔡盼盼, 王立, 等. 超声辅助酶法提取中华鳖裙边胶原蛋白及其热稳定性能[J]. 食品科学,2018,39(2):254−259. [ZOU Y, CAI P P, WANG L, et al. Ultrasonic-assisted enzymatic extraction and thermal stability of collagen from soft-shelled turtle calipash[J]. Food Science,2018,39(2):254−259. doi: 10.7506/spkx1002-6630-201802040
    [18]
    王楠, 王伟, 刘彩琴, 等. 甲鱼蛋白质各组分的营养价值分析[J]. 食品工业科技,2015,36(8):343−347. [WANG N, WANG W, LIU C Q, et al. Fractionation and nutritional properties evaluation of turtle protein[J]. Science and Technology of Food Industry,2015,36(8):343−347. doi: 10.13386/j.issn1002-0306.2015.08.063
    [19]
    孙嘉玲, 文彬, 杨雪梅, 等. 基于 PI3K/AKT/GSK-3β 信号通路探讨鳖甲煎丸调控肝癌细胞 Hep3B 增殖转移的机制[J]. 中华中医药杂志,2021,36(3):1361−1365. [SUN J L, WEN B, YANG X M, et al. Mechanism research of regulation of proliferation and metastasis of hepatoma cells Hep3B by Biejiajian Pill based on PI3K/AKT/GSK-3β signaling pathway[J]. Chinese Journal of traditional Chinese Medicine,2021,36(3):1361−1365.
    [20]
    丁茂鹏, 韦凌霞, 王志旺, 等. 基于网络药理学研究鳖甲煎丸抗肝纤维化的作用机制[J]. 中成药,2021,43(10):2874−2878. [DING M P, WEI L X, WANG Z Y, et al. Study on the mechanism of Biejiajian Pill against liver fibrosis based on network pharmacology[J]. Chinese Traditional Patent Medicine,2021,43(10):2874−2878. doi: 10.3969/j.issn.1001-1528.2021.10.053
    [21]
    王道尊, 汤峥嵘, 谭玉钧. 中华鳖生化组成的分析II—背甲、肌肉中矿物元素的组成[J]. 水生生物学报,1998,22(2):106−111. [WANG D Z, TANG Z R, TAN Y J. Analysis of biochemical composition of Trionyx sinensis II-composition of mineral elements in dorsal shell and muscle[J]. Acta Hydrobiologica Sinica,1998,22(2):106−111. doi: 10.3321/j.issn:1000-3207.1998.02.002
    [22]
    侯风萍, 梁莉, 慕艳红, 等. EDTA 络合滴定法测定脑膜炎球菌多糖疫苗原液中的残留钙离子[J]. 微生物学免疫学进展,2017,45(5):25−28. [HOU F P, LIANG L, MU Y H, et al. Determination of residual content for calcium ion in meningococcal polysaccharide vaccine bulk by compleximetry[J]. Prog in Microbiol Immunol,2017,45(5):25−28.
    [23]
    陆剑锋, 万全, 殷章敏, 等. 中华鳖裙边胶原蛋白的提取及其特征[J]. 水产学报,2010(6):801−808. [LU J F, WAN Q, YIN Z M, et al. Extraction and characteristics of collagen from the skirt of Trionyx sinensis[J]. Journal of Fishers of China,2010(6):801−808.
    [24]
    张强, 黄鑫, 符安卫, 等. 中华鳖裙边胶原蛋白的提取, 鉴定及其理化性质[J]. 食品与发酵工业,2019,45(12):176−182. [ZHANG Q, HUANG X, FU A W, et al. Extraction, identification and physicochemical properties of collagen from the skirt of Trionyx sinensis[J]. Food and Fermentation Industries,2019,45(12):176−182.
    [25]
    LEBON M, REICHE I, GALLET X, et al. Rapid quantification of bone collagen content by ATR-FTIR spectroscopy[J]. Radiocarbon,2016,58(1):131−145. doi: 10.1017/RDC.2015.11
    [26]
    刘闪, 刘良忠, 杨永生, 等. 白鲢鱼骨脱钙工艺优化[J]. 食品与机械,2013,29(5):164−168. [LIU S, LIU L Z, YANG Y S, et al. Process optimization of calcium removal of silver carp fish bone[J]. Food and Machinery,2013,29(5):164−168. doi: 10.3969/j.issn.1003-5788.2013.05.044
    [27]
    周如意, 刘明华. 草鱼鳞盐酸脱钙工艺研究[J]. 农产品加工 ( 上 ) ,2017(4):22−25. [ZHOU R Y, LIU M H. Study on decalcification condition of grass carp scales by hydrochloric acid[J]. Farm Products Processing,2017(4):22−25.
    [28]
    杨晓东, 张杨, 张寿, 等. 胶原蛋白肽的提取及应用研究进展[J]. 食品工业科技,2022,43(9):469−476. [YANG X D, ZHANG Y, ZHANG S, et al. Progress in the extraction and application of collagen peptides[J]. Science and Technology of Food Industry,2022,43(9):469−476.
    [29]
    严秋萍, 邬应龙, 李月娥, 等. 齐口裂腹鱼骨胶原蛋白超声波辅助提取工艺及其特性研究[J]. 食品与机械,2017,33(12):154−159. [YAN Q P, WU Y L, LI Y E, et al. Ultrasonic-assisted extraction process and characteristics analysis of collagen from Schizothorax prenanti bone[J]. Food & Machinery,2017,33(12):154−159. doi: 10.13652/j.issn.1003-5788.2017.12.031
    [30]
    白艳, 刘青梅, 杨性民. 响应面法优化鳗鱼骨可溶性钙提取的研究[J]. 上海海洋大学学报,2011,20(4):613−618. [BAI Y, LIU Q M, YANG X M. Optimization of soluble calcium extraction from eel bone by response surface methodology[J]. Journal of Shanghai Ocean University,2011,20(4):613−618.
    [31]
    赵然, 曹敏杰, 王晶, 等. 水产动物源胶原蛋白的提取及应用研究进展[J]. 食品安全质量检测学报,2020,11(22):8157−8165. [ZHAO R, CAO M J, WANG J, et al. Advances in the extraction and application of aquatic animal collagen[J]. Journal of Food Safety and Quality,2020,11(22):8157−8165. doi: 10.19812/j.cnki.jfsq11-5956/ts.2020.22.003
    [32]
    崔帅, 颜冰, 楼盈凯, 等. 果蔬汁的超声波杀菌与品质变化研究[J]. 饮料工业,2019,22(6):15−20. [CUI S, YAN B, LOU Y K, et al. Ultrasound sterilization on fruit and vegetable juice and quality change[J]. Beverage Industry,2019,22(6):15−20. doi: 10.3969/j.issn.1007-7871.2019.06.006
    [33]
    TIWARI B K. Ultrasound: A clean, green extraction technology[J]. TrAC Trends in Analytical Chemistry,2015,71:100−109. doi: 10.1016/j.trac.2015.04.013
    [34]
    周瑞, 李若男, 周丽莎, 等. 暗纹东方鲀鱼皮胶原蛋白的提取及其特性[J]. 水产学报,2020,44(8):1349−1359. [ZHOU R, LI R N, ZHOU L S, et al. Extraction and characteristics of collagens from the skin of puffer fish (Tetrodontiformes fasciatus)[J]. Journal of Fisheries of China,2020,44(8):1349−1359.
    [35]
    DRZEWIECKI K E, GRISHAM D R, PARMAR A S, et al. Circular dichroism spectroscopy of collagen fibrillogenesis: A new use for an old technique[J]. Biophysical Journal,2016,111(11):2377−2386. doi: 10.1016/j.bpj.2016.10.023
  • Cited by

    Periodical cited type(1)

    1. 高子琪,刘秀嶶,李泽林,范方宇,王翰墨,田浩,牛之瑞. 咖啡风味研究动态文献计量学可视化分析. 食品工业科技. 2024(22): 225-235 . 本站查看

    Other cited types(2)

Catalog

    Article Metrics

    Article views (180) PDF downloads (14) Cited by(3)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return