LI Hui, LI Li. Research Progress on the Determination of Nitrite in Food[J]. Science and Technology of Food Industry, 2022, 43(23): 429−435. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022010006.
Citation: LI Hui, LI Li. Research Progress on the Determination of Nitrite in Food[J]. Science and Technology of Food Industry, 2022, 43(23): 429−435. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022010006.

Research Progress on the Determination of Nitrite in Food

More Information
  • Received Date: January 03, 2022
  • Available Online: September 25, 2022
  • Nitrite is commonly found in meat products, pickles, vegetables and other foods. Due to its toxicity the excessive ingestion of nitrite will harm to human health. Therefore, it is particularly important to apply a fast and reliable method to detect nitrite in food. This paper summarizes the pretreatment methods of food stuffs before detection, and reviews the common methods and the latest research progress of nitrite detection in food at home and abroad in recent years. The principle, detection limit, advantages and disadvantages of analysis of nitrite in food stuffs by spectrophotometry, chromatography, chemiluminescence methods, electrochemical methods and titration analysis methods are summarized in this paper. This paper aims to provide a reference for the scientific and accurate detection of nitrite in various foods.
  • [1]
    鲁煊. N-亚硝基化合物对人体的危害及防治措施研究[J]. 食品研究与开发,2014,35(2):128−130. [LU Xuan. Research on the harm of N-nitroso compounds to human body and its prevention and control measures[J]. Food Research and Development,2014,35(2):128−130. doi: 10.3969/j.issn.1005-6521.2014.02.033
    [2]
    赵苗苗. 食品添加剂亚硝酸盐的利弊[J]. 食品安全导刊,2017(21):64. [ZHAO Miaomiao. Advantages and disadvantages of food additive nitrite[J]. Food Safety Guide,2017(21):64. doi: 10.3969/j.issn.1674-0270.2017.21.051
    [3]
    ZHANG Shuxin, PENG Rong, JIANG Ran, et al. A high-throughput headspace gas chromatographic technique for the determination of nitrite content in water samples[J]. Journal of Chromatography A,2018,1538:104−107. doi: 10.1016/j.chroma.2018.01.026
    [4]
    YAQOOB M, FOLGADO B B, NABI A, et al. Determination of nitrate and nitrite in freshwaters using flow-injection with luminol chemiluminescence detection[J]. Luminescence,2012,27(5):419−425. doi: 10.1002/bio.1366
    [5]
    洪徐华. 饮用水生产过程中亚硝酸盐浓度的影响因素及其变化规律的研究[D]. 杭州: 浙江大学, 2019.

    HONG Xuhua. Research on the influencing factors and variation law of nitrite concentration in drinking water production process[D]. Hangzhou: Zhejiang University, 2019.
    [6]
    SUMA B P, PRASHANTH S A, SURESH K K, et al. A new polyoxometalate/rGO/Pani composite modified electrode for electrochemical sensing of nitrite and its application to food and environmental samples[J]. Materials Chemistry and Physics,2019,229:269−278. doi: 10.1016/j.matchemphys.2019.02.087
    [7]
    JING Jia, LU Wenjing, LI Lin, et al. Orange luminescent carbon dots as fluorescent probe for detection of nitrite[J]. Chinese Journal of Analytical Chemistry,2019,47(4):560−566. doi: 10.1016/S1872-2040(19)61155-2
    [8]
    ZHANG Fengyuan, ZHU Xinyue, JIAO Zhijuan, et al. Sensitive naked eye detection and quantification assay for nitrite by a fluorescence probe in various water resources[J]. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2018,200:275−280. doi: 10.1016/j.saa.2018.04.025
    [9]
    ZHANG Min, ZHANG Zhen, YUAN Dongxing, et al. An automatic gas-phase molecular absorption spectrometric system using a UV-LED photodiode based detector for determination of nitrite and total nitrate[J]. Talanta,2011,84(2):443−450. doi: 10.1016/j.talanta.2011.01.048
    [10]
    张小梅. 紫外分光光度法测定食品里的亚硝酸盐含量[J]. 食品安全导刊,2016(27):115. [ZHANG Xiaomei. Nitrite content in food products was determined by UV spectrophotometry[J]. China Food Safety Magazine,2016(27):115. doi: 10.16043/j.cnki.cfs.2016.27.094
    [11]
    朱勇, 应杰, 孙亚米, 等. 紫外分光光度法检测香椿中硝酸盐方法研究[J]. 浙江农业科学,2017,58(1):128−131. [ZHU Yong, YING Jie, SUN Yami, et al. Study on nitrate detection in chinese toon by UV spectrophotometry[J]. Journal of Zhejiang Agricultural Sciences,2017,58(1):128−131.
    [12]
    熊海涛. 催化光度法检测香肠与小青菜中的亚硝酸盐含量[J]. 食品工业,2014,35(8):277−280. [XIONG Haitao. Detection of trace nitrite in sausage and small greens with cat alytic spectrophotometry[J]. Science of Food Industry,2014,35(8):277−280.
    [13]
    王雨蒙. 基于滴定法测定食品中亚硝酸盐的思考与分析[J]. 农家参谋,2020(7):153. [WANG Yumeng. Thinking and analysis of nitrite determination in food based on titration method[J]. The Farmers Consultan,2020(7):153.
    [14]
    FABIANA D B, LUCIANO V, ROSEANE F, et al. Development and validation of a sub-minute capillary zone electrophoresis method for determination of nitrate and nitrite in baby foods[J]. Talanta,2014,122:23−29. doi: 10.1016/j.talanta.2014.01.006
    [15]
    孙凤春. 蔬菜中硝态氮测定方法及累积因素的研究[D]. 长春: 吉林农业大学, 2007.

    SUN Fengchun. Study on the determination method and accumulation factors of nitrate nitrogen in vegetables[D]. Changchun: Jilin Agricultural University, 2007.
    [16]
    BISWAS S, CHOWDHURY B, RAY B C. A novel spectrofluorimetric method for the ultra trace analysis of nitrite and nitrate in aqueous medium and its application to air, water, soil and forensic samples[J]. Talanta,2004,64(2):308−312. doi: 10.1016/j.talanta.2004.02.018
    [17]
    SHU Linglin, HSU J W, FUH M R. Simultaneous determination of nitrate and nitrite in vegetables by poly (vinylimidazole-co-ethylene dimethacrylate) monolithic capillary liquid chromatography with UV detection[J]. Talanta,2019,205:120082. doi: 10.1016/j.talanta.2019.06.082
    [18]
    肖琴, 麦淑文, 巫慧妮, 等. 分光光度法测定纯牛奶中亚硝酸盐含量的研究[J]. 粮油食品科技,2013,21(5):81−83. [XIAO Qin, MAI Shuwen, WU Huini, et al. Determination of the content of nitrite in pure milk by spectrophotometry[J]. Science and Technology of Cereals, Oils and Foods,2013,21(5):81−83. doi: 10.3969/j.issn.1007-7561.2013.05.022
    [19]
    MASIC A, SANTOS A T L, ETTER B, et al. Estimation of nitrite in source-separated nitrified urine with UV spectrophotometry[J]. Water Research,2015,85:244−254. doi: 10.1016/j.watres.2015.08.031
    [20]
    CHEN Yinyin, ZHAO Chenxi, YUE Guizhou, et al. A highly selective chromogenic probe for the detection of nitrite in food samples[J]. Food Chemistry,2020,317:126361. doi: 10.1016/j.foodchem.2020.126361
    [21]
    ALI H R H, HASSAN A I, HASSAN Y F, et al. Nitrite fluorometric nanoprobe based on α-MnO2 nanorods functionalized with a fluorescence reporter dye[J]. Microchemical Journal,2021,164:105982. doi: 10.1016/j.microc.2021.105982
    [22]
    殷紫. 可见分光光度法测定酱菜中亚硝酸钠含量[J]. 海峡科技与产业,2016(10):123−124. [YIN Zi. Determination of sodium nitrite in pickles by visible spectrophotometry[J]. Technology and Industry Across the Straits,2016(10):123−124.
    [23]
    陈翠霞, 林珊, 罗小林, 等. 紫外可见分光光度法检测肉质香肠中亚硝酸质量浓度的研究[J]. 现代盐化工,2019,46(3):22−24. [CHEN Cuixia, LIN Shan, LUO Xiaolin, et al. Study on the determination of nitrous acid concentration in meat sausage by UV-visible spectrophotometry[J]. Modern Salt and Chemical Industry,2019,46(3):22−24. doi: 10.3969/j.issn.1005-880X.2019.03.009
    [24]
    吴兴兴, 唐力, 朱怡君, 等. 分光光度法测定药用辅料中亚硝酸盐的含量[J]. 生物化工,2021,7(2):83−85. [WU Xingxing, TANG Li, ZHU Yijun, et al. Nitrite in pharmaceutical excipients and the development of its analytical method[J]. Biological Chemical Engineering,2021,7(2):83−85. doi: 10.3969/j.issn.2096-0387.2021.02.023
    [25]
    LO H S, LO K W, YEUNG C F, et al. Rapid visual and spectrophotometric nitrite detection by cyclometalated ruthenium complex[J]. Analytica Chimica Acta,2017,990:135−140. doi: 10.1016/j.aca.2017.07.018
    [26]
    ALI A E, BEHZAD R, SIYAVAH N. Simultaneous spectrophotometric determination of nitrite and nitrate by flow injection analysis[J]. Analytica Chimica Acta,2004,110(1):123−128.
    [27]
    霍欢. 催化光度法测定痕量甲醛和亚硝酸根的研究与应用[D]. 湘潭: 湘潭大学, 2007.

    HUO Huan. Study and application of catalytic spectrophotometric determination of trace formaldehyde and nitrite[D]. Xiangtan: Xiangtan University, 2007.
    [28]
    赵桦萍, 陈伟, 李涛. 催化动力学光度法测定肉制品中亚硝酸盐含量[J]. 中国调味品,2016,41(6):139−141. [ZHAO Huaping, CHEN Wei, LI Tao. Catalytic kinetic spectrophotometry determination of nitrite content in meat products[J]. China Condiment,2016,41(6):139−141.
    [29]
    杨兵, 彭传友, 彭斌, 等. 水中亚硝酸盐氮测定条件优化[J]. 光谱实验室,2011,28(6):2952−2956. [YANG Bin, PENG Chuanyou, PENG Bin, et al. Optimization of conditions for the determination of nitrite nitrogen in water[J]. Chinese Journal of Spectroscopy Laboratory,2011,28(6):2952−2956. doi: 10.3969/j.issn.1004-8138.2011.06.046
    [30]
    王秋花. 荧光光度法测定食品中亚硝酸盐的研究与应用[D]. 北京: 中国食品药品检定研究院, 2015.

    WANG Qiuhua. Research and application of fluorescence spectrophotometry for the determination of nitrite in food[D]. Beijing: China Institute for Food and Drug Control, 2015.
    [31]
    张蓓, 何浩, 吴海智, 等. 顶空-气相色谱法测定婴幼儿配方食品和辅助食品中亚硝酸盐[J]. 中国乳品工业,2022,50(2):54−58. [ZHANG Bei, HE Hao, WU Haizhi, et al. Determination of nitrite in formula and complementary foods for infants and young children by headspace-gas chromatography[J]. China Dairy Industry,2022,50(2):54−58. doi: 10.19827/j.issn1001-2230.2022.02.012
    [32]
    MARKUS N, SANDRA L, BERNARD R, et al. Identification of nitrite treated tuna fish meat via the determination of nitrous oxide by head space-gas chromatography/mass spectrometry[J]. F1000Research,2019,8:711. doi: 10.12688/f1000research.19304.1
    [33]
    FOUZIA A, SHINGO S, YUIKO T H, et al. Partition/ion-exclusion chromatographic ion stacking for the analysis of trace anions in water and salt samples by ion chromatography[J]. Analytical Sciences,2018,34(3):369−373. doi: 10.2116/analsci.34.369
    [34]
    HU Jingli, CHRISTISON T, ROHRER J. Determination of dimethylamine and nitrite in pharmaceuticals by ion chromatography to assess the likelihood of nitrosamine formation[J]. Heliyon,2021,7(2):e06179. doi: 10.1016/j.heliyon.2021.e06179
    [35]
    王小芳, 樊继彩, 任韧. 高效液相色谱-荧光检测法测定婴幼儿奶粉中的亚硝酸盐[J]. 中国卫生检验杂志,2021,31(7):808−810. [WANG Xiaofang, FAN Jicai, REN Ren. Rapid determination of nitrite in infant milk powder by high performance liquid chromatography with fluorescence detection[J]. Chinese Journal of Health Laboratory Technology,2021,31(7):808−810.
    [36]
    DESAI P R, MEHTA P J, CHOKSHI A B. Liquid chromatographic method development for quantification of inorganic nitrite and nitrate impurities from nitroglycerin drug substance by using ion-pair reagents with liquid-liquid extraction technique[J]. Journal of Chromatographic Science,2019(1):1.
    [37]
    李克宏, 白彦坤, 周巍, 等. 高效毛细管电泳检测植物蛋白食品中的特征蛋白质[J]. 食品科学,2014,35(12):124−127. [LI Kehong, BAI Yankun, ZHOU Wei, et al. Detection of characteristic proteins in vegetable protein foods by high performance capillary electrophoresis[J]. Food Science,2014,35(12):124−127. doi: 10.7506/spkx1002-6630-201412024
    [38]
    闻静, 李薇, 杨涓. 毛细管电泳在氨基酸分析检测中的应用研究[J]. 轻工科技,2020,36(2):111−113. [WEN Jing, LI Wei, YANG Juan. Application of capillary electrophoresis in amino acid analysis and detection[J]. Light Industry Science and Technology,2020,36(2):111−113.
    [39]
    程珂. 水质亚硝酸盐氮的检测方法综述[J]. 河南化工,2020,37(3):13−15. [CHENG Ke. Summary on detection methods of nitrite nitrogen in water quality[J]. Henan Chemical Industry,2020,37(3):13−15. doi: 10.14173/j.cnki.hnhg.2020.03.004
    [40]
    刘丰奎, 汪雨, 钱春燕, 等. 超声辅助提取-气相分子吸收光谱法测定火腿中亚硝酸盐[J]. 分析科学学报,2022,38(1):89−93. [LIU Fengkui, WANG Yu, QIAN Chunyan, et al. Determination of nitrite in ham by ultrasonic-assisted extraction-gas phase molecular absorption spectrometry[J]. Journal of Analytical Science,2022,38(1):89−93. doi: 10.13526/j.issn.1006-6144.2022.01.016
    [41]
    王燕, 刘素美, 任景玲, 等. 化学发光法测定天然水体中低浓度硝酸盐和亚硝酸盐的含量[J]. 海洋科学,2011,35(5):95−99. [WANG Yan, LIU Sumei, REN Jingling, et al. Chemiluminescent measurement of low concentrations of nitrate and nitrite[J]. Marine Sciences,2011,35(5):95−99.
    [42]
    KANDA Y, TAIRA M. Flow-injection analysis method for the determination of nitrite and nitrate in natural water samples using a chemiluminescence NOx monitor[J]. Analytical Sciences,2003,19(5):695−699. doi: 10.2116/analsci.19.695
    [43]
    连欢, 李金涛, 刘凤平, 等. 氧化锌纳米材料修饰电极对香肠中亚硝酸盐的测定[J]. 中国食品添加剂,2021,32(4):86−90. [LIAN Huan, LI Jintao, LIU Fengping, et al. Determination of nitrite in sausage by ZnO nanomaterial modified electrode[J]. China Food Additives,2021,32(4):86−90. doi: 10.19804/j.issn1006-2513.2021.04.014
    [44]
    CHENG Zhenyu, SONG Haiyan, ZHANG Xianfa, et al. Morphology control of ZnO by adjusting the solvent and non-enzymatic nitrite ions electrochemical sensor constructed with stir bar-shaped ZnO/Nafion nanocomposite[J]. Sensors and Actuators B:Chemical,2021,346:130525. doi: 10.1016/j.snb.2021.130525
    [45]
    TURDEAN G L, SZABO G. Nitrite detection in meat products samples by square-wave voltammetry at a new single walled carbon naonotubes-myoglobin modified electrode[J]. Food Che-mistry,2015,179:325−330. doi: 10.1016/j.foodchem.2015.01.106
    [46]
    阙小峰, 方志成, 徐良, 等. 石墨烯修饰电极的制备及对食品中亚硝酸盐的检测[J]. 食品科技,2021,46(8):313−318. [QUE Xiaofeng, FANG Zhicheng, XU Liang, et al. Preparation of graphene modified electrode and detection of nitrite in food[J]. Food Science and Technology,2021,46(8):313−318. doi: 10.13684/j.cnki.spkj.2021.08.048
    [47]
    张怡. 基于氧化碳纤维纸的亚硝酸盐电化学传感器的构建[D]. 咸阳: 西北农林科技大学, 2021.

    ZHANG Yi. Construction of nitrite electrochemical sensor based on oxidized carbon fiber paper[D]. Xianyang: Northwest A & F University, 2021.
    [48]
    CAO Liping, KANG Zewen, DING Qi, et al. Rapid pyrolysis of Cu2+-polluted eggshell membrane into a functional Cu2+-Cu+/biochar for ultrasensitive electrochemical detection of nitrite in water[J]. Science of the Total Environment,2020,723:138008. doi: 10.1016/j.scitotenv.2020.138008
    [49]
    YANG Y, ZHANG J, LI Y W, et al. Ni nanosheets evenly distributed on MoS2 for selective electrochemical detection of nitrite[J]. Colloids and Surfaces A: Physicochemical and Engineering Aspects,2021,625:126865. doi: 10.1016/j.colsurfa.2021.126865
    [50]
    ZHOU Yang, MA Mingyu, HE Hanping, et al. Highly sensitive nitrite sensor based on AuNPs/RGO nanocomposites modified graphene electrochemical transistors[J]. Biosensors and Bioelectronics,2019,146:111751. doi: 10.1016/j.bios.2019.111751
    [51]
    周石洋, 陈玲. 滴定法测定食品中亚硝酸盐含量的研究[J]. 福建分析测试,2012,21(1):42−45. [ZHOU Shiyang, CHEN Ling. Determining the content of nitrite in food through titration method[J]. Fujian Analysis Testing,2012,21(1):42−45. doi: 10.3969/j.issn.1009-8143.2012.01.012
    [52]
    范美霞. 几种腌(腊)制食品中亚硝酸盐含量的检测[J]. 肉类工业,2014(10):33−36. [FAN Meixia. Determination of nitrite content in several pickled (waxed) foods[J]. Meat Industry,2014(10):33−36. doi: 10.3969/j.issn.1008-5467.2014.10.011
  • Related Articles

    [1]XU Lan-ying, JIN Li, XU Yin, ZHANG Yi-fan, LIU Si-si, LONG Tao. Adaptability Study of the Complexation and Color Rendering Systems on Determination of Total Flavonoids in Kunlun Coreopsis tinctoria by Spectrophotometry[J]. Science and Technology of Food Industry, 2020, 41(13): 247-252. DOI: 10.13386/j.issn1002-0306.2020.13.039
    [2]ZHU Mao-mao, WANG Gang, FENG Liang. Screen of AGEs Specific Binding Components in Milk Powder by Cell Membrane Immobilized Chromatography[J]. Science and Technology of Food Industry, 2018, 39(22): 257-261,269. DOI: 10.13386/j.issn1002-0306.2018.22.045
    [3]LI Lin, HUANG Ping-ping, LI Hua-min, WANG Ya-ping, NI Wei-wei. Effect of Different Extraction Methods on the Content of EPA and DHA in Fish Oil by Gas Chromatography[J]. Science and Technology of Food Industry, 2018, 39(15): 255-259. DOI: 10.13386/j.issn1002-0306.2018.15.045
    [4]YANG Yu-kun, SONG Jia, QIAO Shen, GUO Yuan-yuan, CHANG Yuan-yuan, WANG Xing-hua. Simultaneous determination of lactic acid and acetic acid in enzyme solution by high performance liquid chromatography[J]. Science and Technology of Food Industry, 2018, 39(10): 246-250. DOI: 10.13386/j.issn1002-0306.2018.10.045
    [5]MAO Shan-qiao, YANG Feng, HUANG Yong-chun. Analyze the influence of hydrodynamic cavitation on sucrose by high performance liquid chromatography[J]. Science and Technology of Food Industry, 2017, (01): 189-192. DOI: 10.13386/j.issn1002-0306.2017.01.028
    [6]HAN Ting-ting, CUI He, DUAN Xiao-juan, SONG Tian, JI Hong-wei, LI Hui-xin, CAI Feng, ZHU Qian-lin. Determination of four anions in alcohol and methanol by ion chromatography[J]. Science and Technology of Food Industry, 2016, (11): 284-288. DOI: 10.13386/j.issn1002-0306.2016.11.050
    [7]HU Xiao-ke, SUN Dan-hong, WEN Jun. Determination of aflatoxins B1, B2, G1, and G2 in foods by high performance liquid chromatography with pre-column derivation[J]. Science and Technology of Food Industry, 2015, (12): 49-52. DOI: 10.13386/j.issn1002-0306.2015.12.001
    [8]GUO Chun-hai, LIU Bao-sheng, MAO Pei-xin. Determination of residues of NVP in PVPP by high performance liquid chromatography[J]. Science and Technology of Food Industry, 2015, (04): 76-78. DOI: 10.13386/j.issn1002-0306.2015.04.007
    [9]TANG Le-pan, ZHOU Yong-yan, YU Ai-nong. Comparison of UV spectrophotometry and HPLC in determination of ascorbic acid in Maillard reaction[J]. Science and Technology of Food Industry, 2014, (10): 79-82. DOI: 10.13386/j.issn1002-0306.2014.10.008
    [10]WU Yan-lei, LIN Xiao-yang, HU Jing, ZHAO Bo, ZHU Yong-hong. Fast determination of 18 synthetic colors in foods by high performance liquid chromatography[J]. Science and Technology of Food Industry, 2013, (24): 49-52. DOI: 10.13386/j.issn1002-0306.2013.24.024
  • Cited by

    Periodical cited type(7)

    1. 孟新涛,许铭强,张婷,古丽米热·祖努纳,牛逍瞳,郭金宝,刘国庆,马燕. 基于GC-IMS技术分析新疆不同品种核桃油挥发性成分的差异. 中国油脂. 2025(03): 102-109 .
    2. 古丽米热·祖努纳,孟新涛,叶朵朵,付慧鑫,乔雪,乔雅洁,张婷. 不同储藏温度下鲜羊肉品质及风味的变化. 现代食品科技. 2025(03): 203-221 .
    3. 乔雪,乔雅洁,付慧鑫,孟新涛,张婷. 低压静电场辅助解冻对牛肉品质的影响. 食品工业科技. 2024(17): 48-56 . 本站查看
    4. 杨秉坤,剧柠,丁雨红,郭蓉,龚绵红. 沙棘酸奶挥发性风味物质的GC-IMS表征. 食品工业科技. 2023(13): 308-315 . 本站查看
    5. 张凡,张宇帆,苏心悦,徐文雅,安焕炯,马倩云,孙剑锋,王颉,王文秀. 基于顶空气相离子迁移谱的干腐病马铃薯挥发性成分分析. 食品科学. 2022(06): 317-323 .
    6. 王福成,米思,李劲松,王雨行,王向红. 基于气相色谱-离子迁移谱技术分析不同包装条件对黄瓜风味的影响. 食品工业科技. 2022(08): 296-304 . 本站查看
    7. 马姗,于文龙,焦英帅,刘卫华,王向红. 不同减菌处理对凡纳对虾贮藏期间品质的影响. 食品科技. 2022(03): 116-124 .

    Other cited types(3)

Catalog

    Article Metrics

    Article views (848) PDF downloads (80) Cited by(10)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return