Pharmacodynamic Study of Carbon Quantum Dot on <i>Helicobacter pylori</i> and Prediction of Its Mechanism

ZHANG Xiwu; HU Zhixin; WANG Yahong; DOU Jinjin

doi:10.13386/j.issn1002-0306.2021120307

Volume 43 Issue 20

Oct. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(20): 361-371. > DOI: 10.13386/j.issn1002-0306.2021120307

ZHANG Xiwu, HU Zhixin, WANG Yahong, et al. Pharmacodynamic Study of Carbon Quantum Dot on Helicobacter pylori and Prediction of Its Mechanism[J]. Science and Technology of Food Industry, 2022, 43(20): 361−371. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120307.

Citation:

PDF (11709 KB)

Pharmacodynamic Study of Carbon Quantum Dot on Helicobacter pylori and Prediction of Its Mechanism

1.
Heilongjiang University of Chinese Medicine, Harbin 150040, China
2.
Harbin Chengcheng Institute of Life and Matter, Harbin 150500, China
3.
First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, China

More Information

Received Date: December 28, 2021
Available Online: August 08, 2022

Graphical Abstract

Abstract

Abstract

Objective: To investigate the in vivo inhibitory effects of carbon quantum dot lady wine (lady wine for short) and carbon quantum dot alkaline liquor (alkaline wine for short) on Helicobacter pylori in mice, and speculation of the mechanism of action of wine by network pharmacology. Methods: 70 KM mice were randomly divided into blank control group, model group, positive drug group, high-dose and low-dose group of lady wine, and high and low-dose alkaline alcohol group. Except blank control group, mouse in other groups were gavaged with bacterial suspension to establish Helicobacter pylori infection model. The changes in general physical signs such as body weight, the general morphological changes of mouse stomach, urease intensity and pathological sections were used as evaluation indicators to evaluate the effect of inhibiting Hp. Using network pharmacological analysis to screen the beneficial ingredients in wine through literature, and find its targets on the TCMSP platform, collect Hp disease targets through GeneCards database, take the intersection of the two, and combine the targets Point import uses Cytoscape 3.7.1 software to construct a protein-protein interaction (PPI) network diagram and the “wine-component-target” network diagram, and then perform GO and KEGG enrichment analysis. Results: Compared with the model group, the strong positive rate and total positive rate of Hp in positive control group, lady wine high-dose and low-dose groups and alkaline wine high-dose groups were significantly decreased (P<0.05), it could ignificantly reduce the positive rate of urease detection (P<0.05), bacterial colonization and reduce inflammation, and the efficacy was dose-dependent. Compared with the model group, the inflammatory cells and inflammation degree were significantly reduced in lady wine and alkaline wine low-dose groups. In the high-dose group, there was almost no inflammatory reaction, and the distribution of gastric gland cells was clear and neat. Screened out 21 trace health components in wine, corresponding to 315 targets, 905 disease-related targets and 74 intersection targets were retrieved. Finally, 53 core targets such as CUL4B, ACTB, PARP1, etc. were obtained, involving various physiological processes such as the response to bacteria-derived molecules, regulation of defense responses, lysosomes, and oxidoreductase activity, and mainly regulate IL-17 signaling pathway, TNF signaling pathway, MAPK signaling pathway and PI3K-Akt signaling pathway, etc. Conclusion: Carbon quantum dot wine had a good inhibitory effect on Hp, and the trace components in the wine acted on multiple targets through multiple pathways to exert antibacterial effect, providing theoretical support for the inhibitory effect of wine on Hp.
- KM mice,
- carbon quantum dot lady wine,
- carbon quantum dot alkaline liquor,
- Helicobacter pylori,
- network pharmacology,
- mechanism

FullText(HTML)

References (34)

References

[1]	胡伏莲. 幽门螺杆菌感染治疗中的问题及其处理策略[J]. 中国中西医结合杂志,2010,30(3):229−232. [HU Fulian. Problems in the treatment of Helicobacter pylori infection and its treatment strategies[J]. Chinese Journal of Integrated Traditional Chinese and Western Medicine,2010,30(3):229−232.
[2]	IRAMI A-F, JOSÉ B-N, MOHANA P L A, et al. Prevalence of Helicobacter pylori infection in advanced gastric carcinoma[J]. Arquivos De Gastroenterologia,2006,43(4):288−292. doi: 10.1590/S0004-28032006000400009
[3]	刘婷婷, 张颖秋, 朱樑. 幽门螺旋杆菌与肝病的研究进展[J]. 第二军医大学学报,2016,37(6):744−749. [LIU Tingting, ZHANG Yingqiu, ZHU Liang. Research progress of Helicobacter pylori and liver disease[J]. Journal of Second Military Medical University,2016,37(6):744−749.
[4]	王佳静, 邵洲杰, 叶鸿雁. 幽门螺杆菌传播和定植机制研究进展[J]. 检验医学,2020,35(3):282−286. [WANG Jiajing, SHAO Zhoujie, YE Hongyan. Research progress on the transmission and colonization mechanism of Helicobacter pylori[J]. Laboratory Medicine,2020,35(3):282−286. doi: 10.3969/j.issn.1673-8640.2020.03.020
[5]	崔璨璨, 李长锋, 张斌. 幽门螺旋杆菌感染治疗方案的研究现状和进展[J]. 吉林大学学报(医学版),2017,43(6):1287−1290. [CUI Cancan, LI Changfeng, ZHANG Bin. Research status and progress in therapeutic schedule of Helicobacter pylori infection[J]. Journal of Jilin University (Medicine Edition),2017,43(6):1287−1290. doi: 10.13481/j.1671-587x.20170642
[6]	刘梦真, 刘群. 根除幽门螺旋杆菌影响因素的研究进展[J]. 医学研究生学报,2019,32(12):1339−1344. [LIU Mengzhen, LIU Qun. Research progress on factors affecting Helicobacter pylori eradication[J]. Journal of Medical Postgraduates,2019,32(12):1339−1344.
[7]	马瑾煜, 刘珊瑚, 焦顺刚, 等. 丁香属植物的化学成分和药理活性研究进展[J]. 中国中药杂志,2020,45(8):1833−1843. [MA Jinyu, LIU Shanhu, JIAO Shungang, et al. Research progress on the chemical constituents and pharmacological activities of Syringa plants[J]. China Journal of Chinese Materia Medica,2020,45(8):1833−1843. doi: 10.19540/j.cnki.cjcmm.20200224.202
[8]	吴玲, 郑琴, 张科楠, 等. 木部类、果部类和草部类药食同源中药安全性评价研究进展[J]. 中草药,2019,50(10):2505−2512. [WU Ling, ZHENG Qin, ZHANG Kenan, et al. Research progress on the safety evaluation of Chinese medicines of arborescens, fruit and herb medicines and foods[J]. Chinese Herbal Medicine,2019,50(10):2505−2512. doi: 10.7501/j.issn.0253-2670.2019.10.035
[9]	唐雪阳, 谢果珍, 周融融, 等. 药食同源的发展与应用概况[J]. 中国现代中药,2020,22(9):1428−1433. [TANG Xueyang, XIE Guozhen, ZHOU Rongrong, et al. Development and application of "One root of medicine and food"[J]. Modern Chinese Medicine,2020,22(9):1428−1433.
[10]	张涛, 郭一民, 李卓扬, 等. 碳量子点诱导人体肝癌细胞凋亡的研究[J]. 郑州大学学报 ( 工学版 ) ,2020,41(5):1−7. [ZHANG Tao, GUO Yimin, LI Zhuoyang, et al. Study on the apoptosis of human hepatocarcinoma cells induced by carbon quantum dots[J]. Journal of Zhengzhou University (Engineering Science Edition),2020,41(5):1−7.
[11]	JUANNI C, HUI P, XIUPING W, et al. Graphene oxide exhibits broad-spectrum antimicrobial activity against bacterial phytopathogens and fungal conidia by intertwining and membrane perturbation [J]. Nanoscale, 2014, 6(3): 1879-1889.
[12]	刘源才, 单义民, 赖富丽, 等. 酒的作用与现代医学应用[J]. 酿酒科技,2014(12):128−131. [LIU Yuancai, SHAN Yimin, LAI Fuli, et al. The role of wine with modern medical applications[J]. Liquor-Making Science & Technology,2014(12):128−131.
[13]	ABRAMSON J L, WILLIAMS S A, KRUMHOLZ H M, et al. Moderate alcohol consumption and risk of heart failure among older persons[J]. JAMA,2001,285(15):1971−1977. doi: 10.1001/jama.285.15.1971
[14]	吴月国, 赵铮蓉, 吴蓓丽, 等. 具有增强免疫作用的铁皮石斛灵芝酒的研制[J]. 食品研究与开发,2019,40(21):115−118. [WU Yueguo, ZHAO Zhengrong, WU Beili, et al. Study on dendrobium officinale Ganoderma lucidum wine with enhanced immunity[J]. Food Research and Development,2019,40(21):115−118.
[15]	中华人民共和国国际卫生和计划生育委员会. GB 16740-2014, 食品安全国家标准保健食品[S]. 2014 National Health and Family Planning Commission of the People's Republic of China. GB 16740-2014 China food safety national standard. Health food[S]. 2014.
[16]	付欢, 陈雪峰, 赵燕妮, 等. 白酒成分分析的研究进展[J]. 食品与发酵工业,2021,47(21):320−327. [FU Huan, CHEN Xuefeng, ZHAO Yanni, et al. Research progress in liquor composition analysis[J]. Food and Fermentation Industries,2021,47(21):320−327.
[17]	DONGRUI Z, JINYUAN S, BAOGUO S, et al. Intracellular antioxidant effect of vanillin, 4-methylguaiacol and 4-ethylguaiacol: Three components in Chinese Baijiu[J]. RSC Advance,2017,7(73):2391−2403.
[18]	黄蕴利, 黄永光, 郭旭. 白酒中的主要生物活性功能成分研究进展[J]. 食品工业科技,2016,37(15):375−379. [HUANG Yunli, HUANG Yongguang, GUO Xu. Research progress of main bioactive functional components in liquor[J]. Science and Technology of Food Industry,2016,37(15):375−379.
[19]	韩艳, 王庆伟, 张琰, 等. 丁香对幽门螺杆菌的体外抑菌作用[J]. 医药导报,2011,30(3):302−303. [HAN Yan, WANG Qingwei, ZHANG Yan, et al. Anti-Helicobacter pylori action of eugenol and essential oil from Eugenia caryophyllata in vitro[J]. Herald of Medicine,2011,30(3):302−303. doi: 10.3870/yydb.2011.03.010
[20]	韩艳, 张琰, 刘新友, 等. 药用植物提取物20种抗幽门螺杆菌的活性研究[J]. 西北国防医学杂志,2011,32(6):413−415. [HAN Yan, ZHANG Yan, LIU Xinyou, et al. Helicobacter pylori eradication with extractive of 20 species of medicinal plants[J]. Northwest National Defense Medical Journal,2011,32(6):413−415.
[21]	覃慧林, 张永峰, 王心怡, 等. 木瓜乙酸乙酯萃取部位对急性胃溃疡小鼠胃黏膜中miR-423-5p、TFF1和p53表达的影响[J]. 中药药理与临床,2016,32(3):80−82. [QIN Huilin, ZHANG Yongfeng, WANG Xinyi, et al. Effects of extracts of papaya ethyl acetate on the expression of miR-423-5p, TFF1 and p53 in the gastric mucosa of mice with acute gastric ulcer[J]. Pharmacology and Clinics of Chinese Materia Medica,2016,32(3):80−82.
[22]	石孟琼, 贺君宇, 王晓, 等. 木瓜总三萜对幽门螺杆菌诱导小鼠胃炎的保护作用研究[J]. 中国中药杂志,2021,46(18):4782−4792. [SHI Mengqiong, HE Junyu, WANG Xiao, et al. Protective effect of total triterpenoids from Chaenomeles speciosa against Helicobacter pylori-induced gastritis in mice[J]. Chinese Journal of Chinese Materia Medica,2021,46(18):4782−4792.
[23]	HAIBO H, MINLU F, HAIYAN X, et al. Total triterpenoids from the fruits of Chaenomeles speciosa exerted gastroprotective activities on indomethacin-induced gastric damage via modulating microRNA-423-5p-mediated TFF/NAG-1 and apoptotic pathways[J]. Food & Function,2020,11(1):662−679.
[24]	蒋百春, 龚瑶琴. 泛素连接酶复合物骨架蛋白CUL4B功能研究进展[J]. 中国科学:生命科学,2018,48(11):1136−1151. [JIANG Baichun, GONG Yaoqin. Research progress on the function of ubiquitin ligase complex backbone protein CUL4B[J]. Science China:Life Sciences,2018,48(11):1136−1151.
[25]	QI M, JIAO M, LI X, et al. Correction: CUL4B promotes gastric cancer invasion and metastasis-involvement of upregulation of HER2[J]. Oncogene,2021,40(42):1075−1085.
[26]	朱屿倩, 吴凌云. U2AF1基因突变调控FOXO3a-Bim信号通路对SKM-1细胞炎症因子表达的影响[J]. 中国实验血液学杂志,2021,29(6):1858−1863. [ZHU Yuqian, WU Lingyun. Effect of U2AF1 mutation to inflammatory cytokine expression in SKM-1 cells through FOXO3a-Bim signaling pathway[J]. Chinese Journal of Experimental Hematology,2021,29(6):1858−1863.
[27]	IWONA B-M, KATARZYNA N, EWA Z, et al. Whole blood ACTB, B2M and GAPDH expression reflects activity of inflammatory bowel disease, advancement of colorectal cancer, and correlates with circulating inflammatory and angiogenic factors: Relevance for real-time quantitative PCR[J]. Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University,2020,29(5):547−556.
[28]	BERG W B V D, MCINNES I B. Th17 cells and IL-17 A-focus on immunopathogenesis and immunotherapeutics[J]. Seminars in Arthritis and Rheumatism,2013,43(2):158−170. doi: 10.1016/j.semarthrit.2013.04.006
[29]	彭卫斌, 杨元生, 容海鹰, 等. 联合益生菌根除幽门螺杆菌对IL-8、IL-10、IL-17的调节效应[J]. 胃肠病学和肝病学杂志,2020,29(7):789−793. [PENG Weibin, YANG Yuansheng, RONG Haiying, et al. Modulatory effects of Helicobacter pylori eradication combined with probiotics on IL-8, IL-10 and IL-17[J]. Journal of Gastroenterology and Hepatology,2020,29(7):789−793. doi: 10.3969/j.issn.1006-5709.2020.07.013
[30]	ADAMSSON J, OTTSJÖ L S, LUNDIN S B, et al. Gastric expression of IL-17A and IFNγ in Helicobacter pylori infected individuals is related to symptoms[J]. Cytokine,2017,99:30−34. doi: 10.1016/j.cyto.2017.06.013
[31]	罗强, 刘杰, 刘志刚. 酱香型白酒中生物活性物质抗幽门螺杆菌代谢物诱导的人胃上皮细胞炎症作用研究[J]. 食品与发酵科技,2020,56(4):11−17. [LUO Qiang, LIU Jie, LIU Zhigang. Anti-inflammatory effects of bioactive substances in Maotai-flavor liquor on human gastric epithelial cells induced by metabolites of Helicobacter pylori[J]. Food and Fermentation Science & Technology,2020,56(4):11−17.
[32]	YASUHIRO M, YASUSHI S, HIROSHI M, et al. Trastuzumab in combination with docetaxel/cisplatin/S-1 (DCS) for patients with HER2-positive metastatic gastric cancer: Feasibility and preliminary efficacy[J]. Cancer Chemotherapy and Pharmacology,2015,76(2):375−382. doi: 10.1007/s00280-015-2807-7
[33]	田华, 黄毓娟. 蒲公英多糖对幽门螺杆菌相关性胃炎大鼠胃黏膜炎性反应及MAPK/ERK通路的影响[J]. 现代中西医结合杂志,2019,28(35):3877−3880. [TIAN Hua, HUANG Yujuan. Effect of dandelion polysaccharide on gastric mucosal inflammatory reaction and MAPK/ERK pathway in rats with Helicobacter pylori-associated gastritis[J]. Modern Journal of Integrated Traditional Chinese and Western Medicine,2019,28(35):3877−3880. doi: 10.3969/j.issn.1008-8849.2019.35.001
[34]	缪亚军, 杨莉. 紫杉醇联合顺铂调控MAPK/NF-κB信号治疗幽门螺杆菌胃癌机制的研究[J]. 东南大学学报(医学版),2018,37(4):706−710. [MIAO Yajun, YANG Li. Study on the mechanism of paclitaxel combined with cisplatin in regulating MAPK/NF-κB signaling in the treatment of Helicobacter pylori gastric cancer[J]. Journal of Southeast University (Medical Edition),2018,37(4):706−710.

Supplements (0)

Cited By

Cited by

Periodical cited type(6)

1.	洪梦杰，景奕文，于白音，张朝玉，石海英，常圣鑫. 基于网络药理学和分子对接探讨葛根素抗炎的关键靶点. 韶关学院学报. 2025(02): 41-46 .
2.	鲁森，王瑞，高雄，林慧纯，陈忠正，张媛媛，陈旭洁，黄秋颜，李斌，林晓蓉. 基于高分辨质谱和网络药理学探究南昆山毛叶红茶的抗炎机理. 食品工业科技. 2024(02): 30-39 . 本站查看
3.	李思蒙，田荣，殷明婧，谷巍. 基于网络药理学和分子对接探讨中药芫花治疗原发性痛经的作用机制. 亚热带植物科学. 2024(01): 31-39 .
4.	李镁娟，张军，张云数，李乾伟，张娜，刘梦娇，张人平. 网络药理学结合分子对接技术揭示芹菜籽抑制痛风的潜在分子机制. 食品与机械. 2024(03): 44-51 .
5.	张淼，黄菲，江思思，刘小芬. 闽台“一条根”类青草药次生代谢产物研究进展. 中国民族民间医药. 2023(16): 62-67 .
6.	殷春燕，董占军，陈江魁. 基于网络药理学和分子对接技术研究花生红衣多酚抗动脉粥样硬化的作用机制. 食品与发酵工业. 2023(20): 242-249 .