Effect of Chlorophyll on the Proliferation of Intestinal Bacteria Based on Flow Cytometry Method

ZHENG Hongli; WANG Xiao; DU Mengxuan; LIU Chang; ZHANG Yan

doi:10.13386/j.issn1002-0306.2021120296

Volume 43 Issue 22

Nov. 2022

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Science and Technology of Food Industry > 2022 > 43(22): 296-302. > DOI: 10.13386/j.issn1002-0306.2021120296

ZHENG Hongli, WANG Xiao, DU Mengxuan, et al. Effect of Chlorophyll on the Proliferation of Intestinal Bacteria Based on Flow Cytometry Method[J]. Science and Technology of Food Industry, 2022, 43(22): 296−302. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120296.

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Effect of Chlorophyll on the Proliferation of Intestinal Bacteria Based on Flow Cytometry Method

1.
College of Food Science and Nutritional Engineering, China Agricultural University, National Engineering Research Centre for Fruit and Vegetable Processing, Beijing 100083
2.
Institute of Microbiology, Chinese Academy of Sciences, State Key Laboratory of Microbial Resources, Beijing 100101

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Received Date: December 27, 2021
Available Online: September 19, 2022

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Abstract

Abstract

In order to explore the effect of chlorophyll on the proliferation of specific intestinal bacteria at the bacterial species level, this study firstly explored the feasibility of flow cytometry (FCM) to count intestinal bacteria and compared it with the dilution plating method. Then, the effects of chlorophyll on the proliferation of Flavonifractor plautii, Bacteroides vulgatus, Bifidobacterium pseudolongum and Lactobacillus murinus were investigated by FCM. The results showed that FCM could detect live bacteria and dead bacteria at the same time. There was a good correlation between the count of live bacteria by FCM and the number of live bacteria determined by the dilution coating method (r>0.8, P<0.001). However, detection efficiency of FCM was higher, and the detection results were closer to the real value. 100 µg/mL chlorophyll highly significantly promoted the proliferation of Flavonifractor plautii (P<0.01), while 300 and 500 µg/mL chlorophyll extremely significantly inhibited the proliferation of Flavonifractor plautii (P<0.001). 100 µg/mL chlorophyll extremely significantly promoted the proliferation of Bacteroides vulgatus (P<0.001), while 300 and 500 µg/mL chlorophyll extremely significantly inhibited the proliferation of Bacteroides vulgatus (P<0.001). Chlorophyll could extremely significantly inhibit the proliferation of Bifidobacterium pseudolongum (P<0.001), and the higher the concentration was, the stronger the inhibitory effect was. Chlorophyll could extremely significantly promote the proliferation of Lactobacillus murinus (P<0.001), and the higher the concentration was, the stronger the promoting effect was. This study would provide important theoretical data for elucidating the effect of dietary chlorophyll on the growth of gut microbiota.
- flow cytometry,
- dilution plating method,
- chlorophyll,
- intestinal bacteria,
- proliferation

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