Extraction Optimization and Its Inoxidizability and Hypoglycemic Properties in Vitro of Polysaccharide from Salvia plebeia R. Br.

GUO Chang; LI Chao; HOU Mingming; BAI Meiyu; ZHOU Congcong; ZHANG Haiyan

doi:10.13386/j.issn1002-0306.2021120280

Volume 43 Issue 20

Oct. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(20): 211-219. > DOI: 10.13386/j.issn1002-0306.2021120280

GUO Chang, LI Chao, HOU Mingming, et al. Extraction Optimization and Its Inoxidizability and Hypoglycemic Properties in Vitro of Polysaccharide from Salvia plebeia R. Br.[J]. Science and Technology of Food Industry, 2022, 43(20): 211−219. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120280.

Citation:

PDF (3066 KB)

Extraction Optimization and Its Inoxidizability and Hypoglycemic Properties in Vitro of Polysaccharide from Salvia plebeia R. Br.

1.
College of Life Sciences, Henan University, Kaifeng 475000, China
2.
Institute of Science and Engineering, Henan Kaifeng Institute of Science and Technology Media College, Kaifeng 475000, China

More Information

Received Date: December 26, 2021
Available Online: August 07, 2022

Graphical Abstract

Abstract

Abstract

Water extraction and alkali extraction were used to optimize the extraction process of polysaccharides Salvia plebeia R. Br.. Antioxidant and hypoglycemic abilities of the two crude polysaccharides were compared in vitro. The results showed that the best process of Salvia plebeia R. Br. polysaccharide by water extraction was as follows: The extraction times were 4 times, the extraction temperature was 100 ℃, the ratio of solid to liquid was 1:20 g/mL, and the yield of polysaccharide was 2.86%. The optimal extraction process of Salvia plebeia R. Br. polysaccharide by alkali extraction method was as follows: Alkali concentration was 0.3 mol/L, extraction temperature was 100 ℃, solid-liquid ratio was 1:15 g/mL, and the yield of polysaccharide was 7.15%. The maximum scavenging rates of the two crude polysaccharides on DPPH free radical were 81.23% and 45.02% respectively, the maximum scavenging rates on ABTS⁺ free radical were 88.70% and 88.07% respectively, and the scavenging rates on superoxide anion ( ${\text{O}}_{\text{2}}^{{-}}$ ) free radical were maintained at about 20% and 25% respectively, the inhibition rate of α-amylase increased in a dose-dependent manner. The scavenging ability of water extracted crude polysaccharide on DPPH free radical and ABTS⁺ free radical was higher than that of alkali extracted crude polysaccharide. Both polysaccharides had certain ${\text{O}}_{\text{2}}^{{-}}$ free radical scavenging activity and α-amylase inhibition. In addition, through the comparison of infrared scanning spectra, it was found that the pyranose and carbonyl groups in the alkali extracted crude polysaccharide were destroyed compared with those in the water extracted crude polysaccharide, which may be the reason for the difference of their biological activities.
- Salvia plebeia R. Br. polysaccharide,
- water extraction,
- alkali extraction,
- inoxidizability,
- hypoglycemic

FullText(HTML)

References (43)

References

[1]	BANG S, HA T K Q, LEE C, et al. Antiviral activities of compounds from aerial parts of Salvia plebeia R. Br.[J]. Journal of Ethnopharmacology,2016,192:398−405. doi: 10.1016/j.jep.2016.09.030
[2]	SEO H W, SUH J H, KYUNG J S, et al. Subacute oral toxicity and bacterial mutagenicity study of a mixture of korean red ginseng (Panax ginseng C. A. Meyer) and Salvia plebeia R. Br. extracts[J]. Toxicological Research,2019,35(3):215−224. doi: 10.5487/TR.2019.35.3.215
[3]	王继锋, 颜娓娓, 徐佳馨, 等. 荔枝草的化学成分及药理作用研究新进展[J]. 湖南中医药大学学报,2018,38(4):482−485. [WANG G F, YAN W, XV J X, et al. Research progress on chemical constituents and pharmacological effects of Salvia plebeia R. Br doi: 10.3969/j.issn.1674-070X.2018.04.030 J]. Journal of Hunan University of Traditional Chinese Medicine,2018,38(4):482−485. doi: 10.3969/j.issn.1674-070X.2018.04.030
[4]	卢柯遐, 郭静. 草药在皮肤病中的应用[J]. 中国民间疗法,2021,29(2):69−71. [LU K X, GUO J. Application of herbal medicine in skin diseases[J]. Chinese Folk Therapy,2021,29(2):69−71.
[5]	王晓明, 于娇, 陈虹, 等. 不同采收期荔枝草中黄酮成分含量的动态变化研究[J]. 中国野生植物资,2010,29(4):34−36,53. [WANG X M, YV J, CHEN H, et al. Dynamic changes of flavonoids in Salvia plebeia R. Br. at different harvest times[J]. Wild Plant Resources in China,2010,29(4):34−36,53.
[6]	NHOEK P, CHAE H S, KIM Y M, et al. Sesquiterpenoids from the aerial parts of Salvia plebeia with inhibitory activities on proprotein convertase subtilisin/kexin type 9 expression[J]. Journal of Natural Products,2021,84(2):220−229. doi: 10.1021/acs.jnatprod.0c00829
[7]	LIANG Y Y, WAN X H, NIU F J, et al. Salvia plebeia R. Br.: An overview about its traditional uses, chemical constituents, pharmacology and modern applications[J]. Biomedicine & Pharmacotherapy,2020,121:109589.
[8]	兰艳素, 王宁宁, 常相雾. 火焰原子吸收法测定荔枝草中金属元素含量[J]. 广州化工,2016,44(22):78−80. [LAN Y S, WANG N N, CHANG X W. Determination of metal elements in Salvia plebeia R. Br. by flame atomic absorption spectroscopy[J]. Guangzhou Chemical Industry,2016,44(22):78−80. doi: 10.3969/j.issn.1001-9677.2016.22.028
[9]	温建辉, 倪付勇, 李明, 等. 荔枝草化学成分的研究[J]. 中成药,2019,41(10):2393−2397. [WEN J J, NI F Y, LI M, et al. Study on chemical constituents of Salvia plebeia R. Br doi: 10.3969/j.issn.1001-1528.2019.10.022 J]. Chinese Patent Medicine,2019,41(10):2393−2397. doi: 10.3969/j.issn.1001-1528.2019.10.022
[10]	亢文佳, 富艳彬, 李达翃, 等. 荔枝草的化学成分研究[J]. 中草药,2015,46(11):1589−1592. [KANG J W, FU Y B, LI D H, et al. Study on chemical constituents of Salvia plebeia R. Br J]. Chinese Herbal Medicine,2015,46(11):1589−1592.
[11]	YUN L, LI D, YANG L, et al. Hot water extraction and artificial simulated gastrointestinal digestion of wheat germ polysaccharide[J]. International Journal of Biological Macromolecules,2019,123:174−181. doi: 10.1016/j.ijbiomac.2018.11.111
[12]	LIN X, JI X, WANG M, et al. An alkali-extracted polysaccharide from Zizyphus jujuba cv. Muzao: Structural characterizations and antioxidant activities[J]. International Journal of Biological Macromolecules,2019,136:607−615. doi: 10.1016/j.ijbiomac.2019.06.117
[13]	吴娟, 欧志荣, 李昭蓉, 等. 稀酸提取羊栖菜多糖的结构及其抗氧化特性研究[J]. 福建农业学报,2019,34(7):842−851. [WU J, OU Z R, LI Z R, et al. Study on structure and antioxidant properties of polysaccharide extracted from Sargassum fusiforme with dilute acid[J]. Fujian Agricultural Journal,2019,34(7):842−851.
[14]	MA J S, LIU H, HAN C R, et al. Extraction, characterization and antioxidant activity of polysaccharide from Pouteria campechiana seed[J]. Carbohydrate Polymers,2020,229:115409. doi: 10.1016/j.carbpol.2019.115409
[15]	LUKOVA P K, KARCHEVA-BAHCHEVANSKA D P, BIVOLARSKI V P, et al. Enzymatic hydrolysis of water extractable polysaccharides from leaves of Plantago major L[J]. Folia Medica,2017,59(2):210−216. doi: 10.1515/folmed-2017-0023
[16]	I-LUNG YU, ZER-RAN YU, MALCOLM KOO, et al. A continuous fractionation of ginsenosides and polysaccharides from panax ginseng using supercritical carbon dioxide technology[J]. Journal of Food Processing and Preservation,2016,40(4):743−748. doi: 10.1111/jfpp.12655
[17]	梁焕秋, 苏德华, 吴勇辉, 等. 凉粉草多糖的碱液提取及其粉剂的喷雾干燥制备[J]. 食品科技,2014,39(5):182−185. [LIANG H Q, SU D H, WU Y H, et al. Extraction of lye polysaccharides and preparation of powder by spray drying method[J]. Food Technology,2014,39(5):182−185. doi: 10.13684/j.cnki.spkj.2014.05.042
[18]	莫开菊, 周志, 汪兴平, 等. 碱液和木瓜蛋白酶预处理对葛仙米多糖提取效果的影响[J]. 食品科学,2007,32(11):194−198. [MO K J, ZHOU Z, WANG X P, et al. Effects of pretreatment with alkaline solution and papain on the extraction of polysaccharide from Gexianmi[J]. Food Science,2007,32(11):194−198. doi: 10.3321/j.issn:1002-6630.2007.11.042
[19]	陈程莉, 董全, 周宇, 等. 金线莲多糖提取及功能研究进展[J]. 食品与机械,2018,34(9):196−200. [CHEN C L, DONG Q, ZHOU Y, et al. Research progress on extraction and function of polysaccharides from Anoectochilus roxburghii[J]. Food and Machinery,2018,34(9):196−200. doi: 10.13652/j.issn.1003-5788.2018.09.039
[20]	梁美娜, 吴明江, 陈胜, 等. 羊栖菜多糖的提取纯化及功能活性研究进展[J]. 食品安全质量检测学报,2021,12(21):8305−8312. [LIANG M N, WU M J, CHEN S, et al. Research progress on extraction, purification and functional activity of Sargassum fusiforme polysaccharide[J]. Journal of Food Safety and Quality Inspection,2021,12(21):8305−8312. doi: 10.19812/j.cnki.jfsq11-5956/ts.2021.21.001
[21]	徐青, 李冰晶, 罗丽平, 等. 蓝莓多糖的提取分离及生物活性研究进展[J]. 现代化工,2019,39(5):38−41. [XV Q, LI B L, LUO L P, et al. Research progress on extraction, separation and bioactivity of Blueberry polysaccharides[J]. Modern Chemical Industry,2019,39(5):38−41. doi: 10.16606/j.cnki.issn0253-4320.2019.05.009
[22]	连俊辉. 花脸香蘑菌丝体多糖的提取、抗氧化性及其益生活性研究[D]. 长春: 吉林大学, 2021 LIAN J H. Study on extraction, antioxidant activity and probiotic activity of polysaccharide from mycelium of Pleurotus ostreatus [D]. Changchun: Jilin University, 2021.
[23]	LIU C, CUI Y, PI F, et al. Extraction, purification, structural characteristics, biological activities and pharmacological applications of acemannan, a polysaccharide from Aloe vera: A review [J]. Molecules (Basel, Switzerland), 2019, 24(8): 1554.
[24]	YU Y, SHEN M, SONG Q, et al. Biological activities and pharmaceutical applications of polysaccharide from natural resources: A review[J]. Carbohydrate Polymers,2018,183:91−101. doi: 10.1016/j.carbpol.2017.12.009
[25]	王海茹, 王雪飞, 姚晶. 黄柏花粉多糖除蛋白方法比较及其对抗氧化功能的影响[J]. 食品科技, 2015, 40(5): 224−228 WANG H R, WANG X F, YAO J. Comparison of protein removal methods of Phellodendron chinense pollen polysaccharide and its effect on anti oxidative function[J]. Food Science and Technology, 2015, 40(5): 224−228.
[26]	魏绍云, 齐慧玲, 王继伦, 等. 苯酚—硫酸法测定白及多糖[J]. 天津化工,2000,33(3):35−36. [WEI S Y, QI H L, WANG J L, et al. Phenol sulfuric acid method for the determination of white and polysaccharide[J]. Tianjin Chemical Industry,2000,33(3):35−36.
[27]	王蔚新, 张俊, 占剑峰, 等. 福白菊胎菊与朵菊粗多糖体外抗氧化活性分析[J]. 黄冈师范学院学报,2020,40(6):9−13. [WANG W X, ZHANG J, ZHAN J F, et al. In vitro antioxidant activity of crude polysaccharides from Fubai chrysanthemum, Foetal chrysanthemum and chrysanthemum[J]. Journal of Huanggang Normal University,2020,40(6):9−13. doi: 10.3969/j.issn.2096-7020.2020.06.02
[28]	姚佳, 黄希莲, 姚玉仙, 等. 响应面法优化多穗石柯粗多糖提取工艺及抗氧化活性分析[J]. 食品科技,2021,46(9):195−201. [YAO J, HUANG X L, YAO Y X, et al. Optimization of extraction process and antioxidant activity of crude polysaccharide from Lycoris polyphylla by response surface methodology[J]. Food Technology,2021,46(9):195−201.
[29]	严尚隆, 潘创, 杨贤庆, 等. 长松藻多糖降解、结构表征及降血糖活性测定[J]. 食品与发酵工,2021,47(18):119−126. [YAN S L, PAN C, YANG X Q, et al. Degradation, structural characterization and hypoglycemic activity of Trehalose polysaccharide[J]. Food and Fermentation Industry,2021,47(18):119−126.
[30]	胡玲玲, 乌雪燕. 枸杞多糖的水提醇沉法工艺优化[J]. 贵州农业科学,2021,49(7):128−133. [HU L L, WU X Y. Degradation, optimization of water extraction and alcohol precipitation of Lycium barbarum polysaccharide[J]. Guizhou Agricultural Sciences,2021,49(7):128−133. doi: 10.3969/j.issn.1001-3601.2021.07.021
[31]	高石花, 黎国庆, 覃江克, 等. 油茶枯多糖的水提工艺及纯化研究[J]. 食品科技,2013,38(4):191−195. [GAO S H, LI G Q, QIN J K, et al. Study on water extraction and purification of Camellia kudzu polysaccharide[J]. Food Technology,2013,38(4):191−195. doi: 10.13684/j.cnki.spkj.2013.04.023
[32]	李晓洁, 曾百慧, 陈慕兰, 等. 酸性茯苓多糖的提取工艺优化[J]. 中国酿造,2018,37(10):158−161. [LI X J, ZENG B H, CHEN M L, et al. Optimization of extraction process of acidic Poria cocos polysaccharide[J]. China Brewing,2018,37(10):158−161. doi: 10.11882/j.issn.0254-5071.2018.10.031
[33]	高宪军, 段涛, 王承明. 碱提棉籽粕多糖工艺研究[J]. 食品工业科技,2010,31(11):258−261. [GAO X J, DUAN T, WANG C M. Study on alkali extraction of polysaccharide from cottonseed meal[J]. Science and Technology of Food Industry,2010,31(11):258−261.
[34]	FENG S, LUAN D, NING K, et al. Ultrafiltration isolation, hypoglycemic activity analysis and structural characterization of polysaccharides from Brasenia schreberi[J]. International Journal of Biological Macromolecules,2019,135:141−151. doi: 10.1016/j.ijbiomac.2019.05.129
[35]	赵利霞. 碎米荠硒多糖的提取、分离纯化、结构鉴定及生物活性研究[D]. 武汉: 华中农业大学, 2006 ZHAO L X. Extraction, purification, structure identification and bioactivity of selenium polysaccharide from shepherd's purse[D]. Wuhan: Huazhong Agricultural University, 2006.
[36]	SONG Y, ZHU M, HAO H, et al. Structure characterization of a novel polysaccharide from Chinese wild fruits (Passiflora foetida) and its immune-enhancing activity[J]. International Journal of Biological Macromolecules,2019,136:324−331. doi: 10.1016/j.ijbiomac.2019.06.090
[37]	尹艳. 两种水溶性大豆多糖的红外光谱分析[J]. 食品研究与开发,2013,34(8):67−68. [YIN Y. Infrared spectrum analysis of two water-soluble soybean polysaccharides[J]. Food Research and Development,2013,34(8):67−68.
[38]	SHIMADA K, KAMPS J A, REGTS J, et al. Biodistribution of liposomes containing synthetic galactose-terminated diacylglyceryl-poly(ethyleneglycol)s[J]. Biochim Biophys Acta,1997,1326(2):329−341. doi: 10.1016/S0005-2736(97)00036-9
[39]	周涛. 当归多糖分离分析、结构鉴定及生物活性的研究[D]. 武汉: 华中科技大学, 2018 ZHOU T. Isolation, analysis, structure identification and bioactivity of Angelica polysaccharide[D]. Wuhan: Huazhong University of Science and Technology, 2018.
[40]	连朋丽. 黑莓籽多糖抗血栓作用机制研究和禹白芷多糖生物活性研究[D]. 开封: 河南大学, 2016 LIAN P L. Study on antithrombotic mechanism of blackberry seed polysaccharide and bioactivity of Yubaizhi polysaccharide[D]. Kaifeng: Henan University, 2016.
[41]	韩丹. 滑子菇多糖提取、结构及体外抗氧化活性研究[D]. 天津: 天津科技大学, 2018 HAN D. Study on extraction, structure and antioxidant activity of Pleurotus ostreatus polysaccharide in vitro[D]. Tianjin: Tianjin University of Science and Technology, 2018.
[42]	YANG Y, JIU-LIANG Z, LU-HONG S, et al. Inhibition mechanism of diacylated anthocyanins from purple sweet potato (Ipomoea batatas L.) against α-amylase and α-glucosidase[J]. Food Chemistry, 2021, 359: 129934.
[43]	张金苹. 探讨不同基线糖化血红蛋白下阿卡波糖和二甲双胍对中国新诊二型糖尿病患者疗效的影响[D]. 北京: 北京协和医学院, 2016 ZHANG J P. Effect of acarbose and metformin on the efficacy of different types of baseline glycosylated hemoglobin in newly diagnosed type two diabetic patients in China [J]. Beijing: Peking Union Medical College, 2016.

Supplements (0)

Cited By

Cited by

Periodical cited type(6)

1.	郭玉龙，邵高耸，史轻舟，许焯，胡定煜，符式瑜. 纳米材料在食品检验鉴定中的应用研究进展. 山东化工. 2024(01): 91-94 .
2.	左海根，黄芷诺，李毛英，袁小珍，杜永琴，刘小玉，陈雨. 核酸适配体在雌二醇分析中的研究进展. 理化检验-化学分册. 2023(07): 862-868 .
3.	唐春花，杨洁，卢晓玲，陈美仑，魏铮，余鹏，赵佳. 甾体激素核酸适配体的筛选与应用. 生物化学与生物物理进展. 2023(09): 2146-2161 .
4.	于开宁，王润忠，刘丹丹. 水环境中新污染物快速检测技术研究进展. 岩矿测试. 2023(06): 1063-1077 .
5.	常嵘，叶巧燕，刘慧敏，郝欣雨，郭洪侠，郑楠. 牛奶中激素检测方法的研究进展. 食品安全质量检测学报. 2022(16): 5235-5243 .
6.	史学丽，高辉，周永红，赵伟. 一种基于适配体传感器的17β-雌二醇定量分析方法. 河北工业科技. 2021(05): 431-437 .