Effects of Exogenous Trisodium Phosphate on Activities of Key Enzymes of Reactive Oxygen Species and Phenylpropane Metabolism and Accumulation of Phenolic Compounds in Jujube Fruits

WEN Xiaoli

doi:10.13386/j.issn1002-0306.2021120242

Volume 43 Issue 17

Aug. 2022

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Science and Technology of Food Industry > 2022 > 43(17): 381-386. > DOI: 10.13386/j.issn1002-0306.2021120242

WEN Xiaoli. Effects of Exogenous Trisodium Phosphate on Activities of Key Enzymes of Reactive Oxygen Species and Phenylpropane Metabolism and Accumulation of Phenolic Compounds in Jujube Fruits [J]. Science and Technology of Food Industry, 2022, 43(17): 381−386. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120242.

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Effects of Exogenous Trisodium Phosphate on Activities of Key Enzymes of Reactive Oxygen Species and Phenylpropane Metabolism and Accumulation of Phenolic Compounds in Jujube Fruits

WEN Xiaoli

College of Chemistry and Life Science, Anshan Normal University, Anshan 114000, China

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Received Date: December 22, 2021
Available Online: July 03, 2022

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Abstract

Abstract

Jujube fruit (cv. Sanxing) were soaked in 0.5 mg/mL trisodium phosphate solution for 10 min after harvest to study its effects on key enzymes activities and phenolic compounds accumulation in reactive oxygen species metabolism and phenylpropanoid pathway during storage at room temperature. Results showed that trisodium phosphate promoted hydrogen peroxide content during the entire storage time and peaked at 4 d and 10 d. Additionally, trisodium phosphate treatment distinctly increased the activities of ascorbate peroxidase and glutathione reductase on days 4~12 d and 4~8 d of storage (P<0.05). Moreover, trisodium phosphate treatment increased the activities of phenylalanine ammonilyase, 4-coumarate/coenzyme A ligase and peroxidase in the middle and late storage period. A ligase and peroxidase activities in jujube fruit and peaked at 6, 8 and 8 d, respectively, which was 1.18, 1.16 and 1.40 times of those in control (P<0.05). The increase of these enzymes activities significantly accelerated total phenols and flavonoids accumulation in jujube fruit on 6~12 d and peaked on 8 d and 10 d, which was 1.38 and 1.29 times of those in control, respectively (P<0.05). These findings suggest that postharvest trisodium phosphate treatment can activate the activities of enzymes to eliminate reactive oxygen species, and enhance the activities of key enzymes and phenolic compounds accumulation in phenylpropanoid metabolism, thus enhancing disease resistance.
- jujube fruit,
- trisodium phosphate,
- reactive oxygen species,
- phenylpropanoid pathway

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[1]	赵晓. 枣果主要营养成分分析[D]. 保定: 河北农业大学, 2009 ZHAO X. Analysis on the main nutritional composition of Chinese jujube[D]. Baoding: Hebei Agricultural University, 2019.
[2]	庞丽. 大枣在休眠期内酶活性动态变化[J]. 绿色科技,2015,12:77−79. [PANG L. Dynamic changes of enzyme activity in jujube during dormancy period[J]. Journal of Green Science and Technology,2015,12:77−79. PANG L. Dynamic changes of enzyme activity in jujube during dormancy period[J]. Journal of Green Science and Technology, 2015, 12: 77-79.
[3]	鲁周民, 刘坤, 闫忠心, 等. 枣果实营养成分及保健作用研究进展[J]. 园艺学报,2010,37(12):2017−2024. [LU Z M, LIU K, YAN Z X, et al. Research status of nutrient component and health functions of Ziziphus jujuba Mill[J]. Acta Horticulturae Sinica,2010,37(12):2017−2024. LU Z M, LIU K, YAN Z X, et al. Research status of nutrient component and health functions of Ziziphus jujuba Mill[J]. Acta Horticulturae Sinica, 2010, 37(12): 2017-2024.
[4]	雷逢超, 钟玉, 张有林, 等. 鲜枣采后生理及贮藏保鲜技术研究进展[J]. 陕西农业科学,2011,57(3):153−157. [LEI F C, ZHONG Y, ZHANG Y L, et al. Research progress of postharvest physiology and storage technology of fresh jujube[J]. Shanxi Journal of Agricultural Sciences,2011,57(3):153−157. doi: 10.3969/j.issn.0488-5368.2011.03.057 LEI F C, ZHONG Y, ZHANG Y L, et al. Research progress of postharvest physiology and storage technology of fresh jujube[J]. Shanxi Journal of Agricultural Sciences, 2011, 57(3): 153-157. doi: 10.3969/j.issn.0488-5368.2011.03.057
[5]	张惠然, 张军. 冬枣采后生理及保鲜技术研究进展[J]. 保鲜与加工,2017,17(1):134−138. [ZHANG H R, ZHANG J. Research progress on postharvest physiology and storage technology of winter jujube[J]. Storage and Process,2017,17(1):134−138. ZHANG H R, ZHANG J. Research progress on postharvest physiology and storage technology of winter jujube[J]. Storage and Process, 2017, 17(1): 134-138.
[6]	张新, 张瑞, 李喜宏, 等. 气调保鲜实验箱对采后灵武长枣贮藏品质的影响[J]. 西华大学学报(自然科学版),2018,3(2):46−50. [ZHANG X, ZHANG R, LI X H, et al. The effect of air conditioning preservation experiment box on the storage quality of the Lingwu long jujube[J]. Journal of Xihua University (Natural Science Edition),2018,3(2):46−50. doi: 10.3969/j.issn.1673-159X.2018.02.009 ZHANG X, ZHANG R, LI X H, et al. The effect of air conditioning preservation experiment box on the storage quality of the Lingwu long jujube[J]. Journal of Xihua University (Natural Science Edition), 2018, 3(2): 46-50. doi: 10.3969/j.issn.1673-159X.2018.02.009
[7]	GE Y H, CHEN Y R, LI C Y, et al. Effect of trisodium phosphate dipping treatment on the quality and energy metabolism of apples[J]. Food Chemistry,2019,274:324−329. doi: 10.1016/j.foodchem.2018.08.142
[8]	王修俊, 刘颖, 邱树毅, 等. 复合磷酸盐食品添加剂对鲜切青苹果保鲜效果的研究[J]. 食品工业科技,2008,29(8):258−260. [WANG X J, LIU Y, QIU S Y, et al. Study on effects of food additives named mixed phosphates on the fresh keeping of fresh-cut blue apples[J]. Science and Technology of Food Industry,2008,29(8):258−260. WANG X J, LIU Y, QIU S Y, et al. Study on effects of food additives named mixed phosphates on the fresh keeping of fresh-cut blue apples[J]. Science and Technology of Food Industry, 2008, 29(8): 258-260.
[9]	魏美林, 陈延儒, 李灿婴, 等. 采后磷酸钠处理对苹果果实黑斑病及活性氧和苯丙烷代谢的影响[C]//2017. 中国食品科学技术学会第十四届年会暨第九届中美食品业高层论坛论文摘要集. 2017: 549−550 WEI M L, CHEN Y R, LI C Y, et al. Effect of trisodium phosphate treatment on black spot of apple fruit and the roles of anti-oxidative enzymes[C]//2017. The 14th Annual conference of Chinese Society of Food Science and Technology and the 9th Sino-US Food Industry Forum Abstracts, 2017: 549−550.
[10]	GE Y H, WEI M L, LI C Y, et al. Effect of trisodium phosphate treatment on black spot of apple fruit and the roles of anti-oxidative enzymes[J]. Physiological and Molecular Plant Pathology,2019,106:226−231. doi: 10.1016/j.pmpp.2019.03.003
[11]	ZHANG J H, LI C Y, WEI M L, et al. Effects of trisodium phosphate treatment after harvest on storage quality and sucrose metabolism in jujube fruit[J]. Journal of the Science of Food and Agriculture,2019,99:5526−5532. doi: 10.1002/jsfa.9814
[12]	常璐璐, 张乐乐, 于有伟, 等. 精氨酸处理对采后冬枣黑斑病的抗病作用[J]. 食品工业科技,2021,42(12):321−326. [CHANG L L, ZHANG L L, YU Y W, et al. Effect of arginine treatment on disease resistance to Aternaria rot of postharvest winter jujube[J]. Science and Technology of Food Industry,2021,42(12):321−326. CHANG L L, ZHANG L L, YU Y W, et al. Effect of arginine treatment on disease resistance to Aternaria rot of postharvest winter jujube[J]. Science and Technology of Food Industry, 2021, 42(12): 321-326.
[13]	陈莲, 林钟铨, 林河通, 等. 安喜布处理对台湾青枣果实果皮活性氧代谢和细胞膜透性的影响[J]. 热带作物学报,2013,34(4):704−709. [CHAN L, LIN Z S, LIN H T, et al. AnsiP-S (1-MCP) treatment on the active oxygen metabolism and membrane permeability in the pericarp of harvested Indian jujube fruit[J]. Chinese Journal of Tropical Crops,2013,34(4):704−709. doi: 10.3969/j.issn.1000-2561.2013.04.022 CHAN L, LIN Z S, LIN H T, et al. AnsiP-S (1-MCP) treatment on the active oxygen metabolism and membrane permeability in the pericarp of harvested Indian jujube fruit[J]. Chinese Journal of Tropical Crops, 2013, 34(4): 704-709. doi: 10.3969/j.issn.1000-2561.2013.04.022
[14]	GE Y H, LI X, LI C Y, et al. Effect of sodium nitroprusside on antioxidative enzymes and the phenylpropanoid pathway in blueberry fruit[J]. Food Chemistry,2019,295:607−612. doi: 10.1016/j.foodchem.2019.05.160
[15]	GE Y H, DENG H W, BI Y, et al. Postharvest ASM dipping and DPI pre-treatment regulated reactive oxygen species metabolism in muskmelon (Cucumis melo L.) fruit[J]. Postharvest Biology and Technology,2015,99:160−167. doi: 10.1016/j.postharvbio.2014.09.001
[16]	GE Y H, WEI M L, LI C Y, et al. Reactive oxygen species metabolism and phenylpropanoid pathway involved in disease resistance against Penicillium expansum in apple fruit induced by ԑ-poly-l-lysine[J]. Journal of the Science of Food and Agriculture,2018,98(13):5082−5088. doi: 10.1002/jsfa.9046
[17]	葛永红, 毕阳. 苯丙噻重氮结合枯草芽孢杆菌B1处理对甜瓜采后主要病害的抑制效果[J]. 食品科学,2008,96(6):428−432. [GE Y H, BI Y. Inhibitory effects of benzothiazole and Bacillas subtilis B1 treatment on main diseases of postharvest muskmelon[J]. Food Science,2008,96(6):428−432. doi: 10.3321/j.issn:1002-6630.2008.06.096 GE Y H, BI Y. Inhibitory effects of benzothiazole and Bacillas subtilis B1 treatment on main diseases of postharvest muskmelon[J]. Food Science, 2008, 96(6): 428-432. doi: 10.3321/j.issn:1002-6630.2008.06.096
[18]	LIU Y Y, GE Y H, BI Y, et al. Effect of postharvest acibenzolar-S-methyl dipping on phenylpropanoid pathway metabolism in muskmelon (Cucumis melo L.) fruits[J]. Scientia Horticulturae,2014,168(3):113−119.
[19]	LIU H X, JIANG W B, BI Y, et al. Postharvest BTH treatment induces resistance of peach (Prunus persica L. cv. Jiubao) fruit to infection by Penicillium expansum and enhances activity of fruit defense mechanisms[J]. Postharvest Biology and Technology,2005,35(3):263−269. doi: 10.1016/j.postharvbio.2004.08.006
[20]	REN Y L, WANG Y F, GE Y H, et al. Postharvest BTH treatment induced disease resistance and enhanced reactive oxygen species metabolism in muskmelon (Cucumis melo L.) fruit[J]. European Food Research and Technology,2012,234:963−971. doi: 10.1007/s00217-012-1715-x
[21]	HAN S K, NAN Y Y, QU W, et al. Exogenous gamma-aminobutyric acid treatment that contributes to regulation of malate metabolism and ethylene synthesis in apple fruit during storage[J]. Journal of Agricultural and Food Chemistry,2018,66:13473−13482. doi: 10.1021/acs.jafc.8b04674
[22]	王璐璐. 1-MCP延缓采后台湾青枣果实衰老及其作用机理研究[D]. 福州: 福建农林大学, 2014 WANG L L. Studies on delaying senescence of harvested indian jujube (Ziziphus mauritiana Lamk) fruits by 1-MCP treatment and its action mechanism[D]. Fuzhou: Agriculture and Forestry University, 2014.
[23]	邓惠文, 毕阳, 葛永红, 等. 采后BTH处理及粉红单端孢 (Trichothecium roseum) 挑战接种对厚皮甜瓜果实苯丙烷代谢活性的诱导[J]. 食品工业科技,2013(1):323−326. [DENG H W, BI Y, GE Y H, et al. Induction of phenylpropanoid metabolic activity in muskmelon fruit by postharvest BTH treatment and challenge inoculation with Trichothecium roseum[J]. Science and Technology of Food Industry,2013(1):323−326. DENG H W, BI Y, GE Y H, et al. Induction of phenylpropanoid metabolic activity in muskmelon fruit by postharvest BTH treatment and challenge inoculation with Trichothecium roseum[J]. Science and Technology of Food Industry, 2013(1): 323-326.
[24]	葛永红, 董雪, 魏美林. 壳聚糖复合多聚赖氨酸对苹果果实青霉病及抗氧化酶和苯丙烷代谢的影响[J]. 食品工业科技,2018,39(13):281−286. [GE Y H, DONG X, WEI M L, et al. Effect of chitosan combined with polylysine on blue mould of apple fruit and antioxidant enzymes and phenylpropanoid pathway[J]. Science and Technology of Food Industry,2018,39(13):281−286. GE Y H, DONG X, WEI M L, et al. Effect of chitosan combined with polylysine on blue mould of apple fruit and antioxidant enzymes and phenylpropanoid pathway[J]. Science and Technology of Food Industry, 2018, 39(13): 281-286.
[25]	张静茹, 张乐乐, 常璐璐, 等. 壳聚糖复合硅酸钠对冬枣采后苯丙烷代谢及病程相关蛋白的影响[J]. 食品工业科技,2020,41(15):268−273, 280. [ZHANG J R, ZHANG L L, CHANG L L, et al. Effect of chitosan combined with sodium silicate on phenylpropanoid metabolism and pathogenesis-related proteins of postharvest winter jujube[J]. Science and Technology of Food Industry,2020,41(15):268−273, 280. ZHANG J R, ZHANG L L, CHANG L L, et al. Effect of chitosan combined with sodium silicate on phenylpropanoid metabolism and pathogenesis-related proteins of postharvest winter jujube[J]. Science and Technology of Food Industry, 2020, 41(15): 268-273, 280.
[26]	VOGT T. Phenylpropanoid biosynthesis[J]. Molecular Plant,2010,3:2−20. doi: 10.1093/mp/ssp106

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