Selection of Amylase Producing Strain by Atmospheric and Room Temperature Plasmas and Its Enzymological Properties

TONG Fan; HUANG Jiaqi; FAN Jianqiang; HUANG Shixin; LONG Teng; WU Peng; HE Wei; DENG Xiaohua; CHEN Shanyi; TANG Xu

doi:10.13386/j.issn1002-0306.2021120194

Volume 43 Issue 20

Oct. 2022

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Science and Technology of Food Industry > 2022 > 43(20): 137-143. > DOI: 10.13386/j.issn1002-0306.2021120194

TONG Fan, HUANG Jiaqi, FAN Jianqiang, et al. Selection of Amylase Producing Strain by Atmospheric and Room Temperature Plasmas and Its Enzymological Properties[J]. Science and Technology of Food Industry, 2022, 43(20): 137−143. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120194.

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Selection of Amylase Producing Strain by Atmospheric and Room Temperature Plasmas and Its Enzymological Properties

1.
Technology Center, China Tobacco Fujian Industrial Co., Ltd., Xiamen 361021, China
2.
Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China

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Received Date: December 19, 2021
Available Online: July 26, 2022

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Abstract

Abstract

Objective: The amylase-producing strain screened on the surface of Yunnan Malong C3F-2016 tobacco leaves were used as the starting strain to mutagenically select high amylase-producing strain and to study the enzymatic properties before and after mutagenesis. Methods: The amylase-producing strain were selected by ARTP technique and the amylase activity was determined by the 3,5-dinitrosalicylic acid (DNS) method before and after mutagenesis, and the effects of different temperatures, pH, metal ions and UV light on the amylase activity were investigated. Results: A mutant strain Bacillus koreensis FS-103 was selected by mutagenesis with improved enzyme activity and stable transmission, and its amylase activity reached 9050 U/mL, which was 90% higher than that of the starting strain. The optimum action temperature of amylase produced by the high-yielding mutant strain FS-103 was 60 ℃ and the optimum action pH5.5, with good stability between 40 and 50 ℃ and pH5~6, Ca²⁺ and Mg²⁺ had a promoting effect on amylase activity, and the effect of UV irradiation on amylase activity was weakened. Conclusion: This method of mutagenic selection of amylase-producing strain was feasible and lays the theoretical foundation for the research and development of enzyme preparations by this type of strain.
- amylase,
- Bacillus koreensis,
- atmospheric pressure room temperature plasma (ARTP),
- mutation breeding,
- enzymatic properties

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References

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