Analysis on the Effects of Grafting on Tea Plant Metabolites Based on Targeted Metabolomics

CHA Fengguan; LIU Ying; GAO Jun; KONG Sheng; ZHAO Yiming; ZHOU Yongchen; ZUO Denghong; LÜ Caiyou; DING Haiqin; ZHENG Wenzhong

doi:10.13386/j.issn1002-0306.2021120126

Volume 43 Issue 21

Oct. 2022

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Science and Technology of Food Industry > 2022 > 43(21): 45-51. > DOI: 10.13386/j.issn1002-0306.2021120126

CHA Fengguan, LIU Ying, GAO Jun, et al. Analysis on the Effects of Grafting on Tea Plant Metabolites Based on Targeted Metabolomics[J]. Science and Technology of Food Industry, 2022, 43(21): 45−51. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120126.

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Analysis on the Effects of Grafting on Tea Plant Metabolites Based on Targeted Metabolomics

CHA Fengguan^{1, 2,},
LIU Ying¹,
GAO Jun^{1, 2, ,},
KONG Sheng^{1, 2},
ZHAO Yiming^{1, 2},
ZHOU Yongchen^{1, 2},
ZUO Denghong²,
LÜ Caiyou¹,
DING Haiqin¹,
ZHENG Wenzhong^{2, 3}

1.
College of Tea, Yunnan Agricultural University, Kunming 650100, China
2.
Cultivation Research Laboratory in Yunnan Modern Agricultural Tea Industry System, Kunming 650201, China
3.
Yunnan Pu'er Tea Tree Seed Farm, Puer 665000, China

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Received Date: December 12, 2021
Available Online: August 29, 2022

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Abstract

Abstract

In order to explore the difference of metabolites before and after tea tree grafting, tea tree grafting was carried out by cutting and grafting method using "Peach-shaped leaf" as the scion and "Short-grafted pekoe" as the rootstock. Substances in tea plants before and after grafting were detected by using broad-targeted metabolomics technology, and multivariate statistical methods such as principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to study the difference of metabolites before and after grafting. The results showed that a total of 804 metabolites were detected in tea samples before and after grafting, of which 105 metabolites were significantly different; Compared with that before grafting, 44 differential metabolites were significantly up-regulated in tea samples after grafting, and 61 differential metabolites were significantly down-regulated, and the number of differential metabolites down-regulated was greater than that up-regulated; In addition, the study on KEGG metabolic pathways revealed that these differential metabolites were mainly distributed in 20 metabolic pathways including purine metabolism, nicotinate and nicotinamide metabolism, glycerol phosphatide metabolism, flavonoid biosynthesis, flavonoid and flavonol biosynthesis, carbon sequestration by photosynthetic organisms, caffeine metabolism, and biosynthesis of secondary metabolites. Among them, there were 24 differential metabolites annotated by KEGG, which were dominated by flavone, nucleotides and their derivatives. In addition, most flavonoids in the pathways were significantly up-regulated, while nucleotides and their derivatives were significantly down-regulated. In conclusion, the content of metabolites of the variety "Peach-shaped leaf" is significantly changed after grafting, which can provide a theoretical reference for the processing and production of grafted tea leaves to a certain extent.
- tea plant grafting,
- broad-target metabolomics,
- differential metabolites,
- metabolic pathways,
- cutting and grafting method

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References

[1]	杨维时. 茶树嫁接研究进展[J]. 茶叶通讯,1985,12(4):21−24. [YANG Weishi. Research progress of tea grafting[J]. Tea Communication,1985,12(4):21−24.
[2]	宁静, 刘文武, 梁国强, 等. 茶树良种嫁接技术研究[J]. 茶叶通讯,2014,41(3):20−23. [NING Jing, LIU Wenwu, LIANG Guoqiang, et al. Study on grafting technology of improved tea varieties[J]. Tea Communication,2014,41(3):20−23. doi: 10.3969/j.issn.1009-525X.2014.03.005
[3]	冷杨, 尚怀国, 陈勋, 等. 我国低产低效老茶园改造技术措施及工作建议[J]. 中国农技推广,2019,35(8):11−13. [LENG Yang, SHANG Huaiguo, CHENG Xun, et al. Technical measures and work suggestions for the transformation of low yield and low efficiency old tea plantations in China[J]. China's Agricultural Technology Promotion,2019,35(8):11−13. doi: 10.3969/j.issn.1002-381X.2019.08.004
[4]	WANG J, JIANG L B, WU R L. Plant grafting: How genetic exchange promotes vascular reconnection[J]. New Phytologist,2016,214(1):56−65.
[5]	HUANG Y, KONG Q S, CHEN F, et al. The history, current statusand future prospects of vegetable grafting in China[J]. Acta Horticulturae,2015,1086:31−39.
[6]	何文, 潘鹤立, 潘腾飞, 等. 果树砧穗互作研究进展[J]. 园艺学报,2017,44(9):1645−1657. [HE Wen, PAN Heli, PAN Tengfei, et al. Research progress of rootstock spike interaction in fruit trees[J]. Journal of Horticulture,2017,44(9):1645−1657. doi: 10.16420/j.issn.0513-353x.2017-0147
[7]	宁静, 杨阳, 梁国强, 等. 嫁接技术在茶树上的应用研究与展望[J]. 茶叶通讯,2014,41(1):30−33. [NING Jing, YANG Yang, LIANG Guoqiang, et al. Application research and prospect of grafting technology in tea[J]. Tea Communication,2014,41(1):30−33. doi: 10.3969/j.issn.1009-525X.2014.01.009
[8]	卢昱宇, 冯伟民, 陈罡, 等. 蔬菜嫁接技术研究进展及应用[J]. 江苏农业科学,2014,42(7):167−169. [LU Yuyu, FENG Weimin, CHEN Gang, et al. Research progress and application of vegetable grafting technology[J]. Jiangsu Agricultural Science,2014,42(7):167−169. doi: 10.3969/j.issn.1002-1302.2014.07.056
[9]	吴姗, 骆耀平. 嫁接茶树氨基酸含量变化及分析[J]. 茶叶,2000,26(2):3. [WU Shan, LUO Yaoping. Changes and analysis of amino acid content in grafted tea[J]. Tea,2000,26(2):3. doi: 10.3969/j.issn.0577-8921.2000.02.006
[10]	梁月荣, 陆建良, 龚淑英, 等. 嫁接对茶树新梢化学成分的影响[J]. 茶叶,2001,27(1):39−40. [LIANG Yuerong, LU Jianliang, GONG Shuying, et al. Effects of grafting on chemical components of tea shoots[J]. Tea,2001,27(1):39−40. doi: 10.3969/j.issn.0577-8921.2001.01.012
[11]	虞昕磊, 艾于杰, 曲凤凤, 等. 代谢组学在研究茶叶品质形成中的应用[J]. 茶叶科学,2018,38(1):20−32. [YU Xinlei, AI Yujie, QU Fengfeng, et al. Metabolomics application in the study of tea quality formation[J]. Journal of Tea Science,2018,38(1):20−32. doi: 10.3969/j.issn.1000-369X.2018.01.003
[12]	吴文亮, 林勇, 黄浩, 等. 代谢组学在茶叶品质与药理研究中的应用进展[J]. 中国农业科技导报,2018,20(10):44−54. [WU Wenliang, LIN Yong, HUANG Hao, et al. Application progress of metabonomics in the study of tea quality and pharmacology[J]. China Agricultural Science and Technology Guide,2018,20(10):44−54.
[13]	周琼琼, 孙威江. 代谢组学技术及其在茶叶研究中的应用[J]. 天然产物研究与开发,2015,27(10):1821−1826. [ZHOU Qiongqiong, SUN Weijiang. Metabonomics technology and its application in tea research[J]. Natural Product Research and Development,2015,27(10):1821−1826. doi: 10.16333/j.1001-6880.2015.10.024
[14]	马成英, 吕美玲, 周珊, 等. 应用UHPLC-QTOF/MS平台结合代谢组学技术研究嫁接对茶叶次生代谢产物的影响[C]. 中国化学会第30届学术年会-第四十三分会: 质谱分析. 2016 MA Chengying, LÜ Meiling, ZHOU Shan, et al. UHPLC-QTOF/MS platform combined with metabonomics technology was used to study the effect of grafting on secondary metabolites of tea[C]. The 30th Annual Meeting of the Chinese Chemical Society-Chapter 43: Mass Spectrometry. 2016.
[15]	邓威威, 范妍冰, 顾辰辰, 等. 油茶砧和茶穗嫁接后苗期叶片形态和次级代谢物含量的变化[J]. 热带亚热带植物学报,2017,25(1):35−42. [DENG Weiwei, FAN Yanbing, GU Chenchen, et al. Changes of leaf morphology and secondary metabolite content of Camellia oleifera after rootstock and tea ear grafting at seedling stage[J]. Journal of Tropical and Subtropical Plants,2017,25(1):35−42. doi: 10.11926/jtsb.3641
[16]	杨凤玲. 低产茶园茶树嫁接试验研究[J]. 云南农业科技,2009(2):6−9. [YANG Fengling. Experimental study on tea grafting in low yield tea garden[J]. Yunnan Agricultural Science and Technology,2009(2):6−9. doi: 10.3969/j.issn.1000-0488.2009.02.001
[17]	陶仕科, 郑新强, 梁月荣. 云南大叶种茶树老桩套袋嫁接及管理技术[J]. 茶叶,2021,47(3):165−168. [TAO Shike, ZHENG Xinqiang, LIANG Yuerong. Bagging grafting and management technology of old piles of Yunnan large leaf tea[J]. Tea,2021,47(3):165−168. doi: 10.3969/j.issn.0577-8921.2021.03.009
[18]	韦持章. 云南大叶茶群体种嫁接乌龙茶品种研究[D]. 南宁: 广西大学, 2012 WEI Zhizhang. Study on grafting oolong tea varieties with Yunnan Daye tea population species[D]. Nanning: Guangxi University, 2012.
[19]	KARUNAKARAN R, LLANGO R. Grafting influence on productivity and drought tolerance of tea clones[J]. The Journal of Agricultural Science,2019,157(3):217−225. doi: 10.1017/S0021859619000480
[20]	TUWEI G, KAPTICH F K K, LANGAT M C, et al. Effects of grafting on tea 2. Drought tolerance[J]. Experimental Agriculture,2008,44(4):537−546. doi: 10.1017/S0014479708006947
[21]	NAGARAJAH S, SOLOMON H R. Grafting for drought resis-tance in clonal tea[J]. Tea Quarterly,1981,50(4):172−174.
[22]	张丽芬, 刘建平. 白化茶树新品种‘景白1号’选育报告[J]. 茶叶,2018,44(3):125−129. [ZHANG Lifen, LIU Jianping. Breeding report of a new albino tea variety 'Jingbai 1'[J]. Tea,2018,44(3):125−129. doi: 10.3969/j.issn.0577-8921.2018.03.002
[23]	FENG L, GAO M J, HOU R Y, et al. Determination of quality constituents in the young leaves of albino tea cultivars[J]. Food Chemistry,2014,155(11):98−104.
[24]	吴姗, 骆耀平. 嫁接两年生茶树新梢主要生化成分的变化[J]. 茶叶,2001,27(3):5. [WU Shan, LUO Yaoping. Changes of main biochemical components in grafted two-year-old tea shoots[J]. Tea,2001,27(3):5. doi: 10.3969/j.issn.0577-8921.2001.03.007
[25]	王文建. 不同砧木嫁接铁观音与茶叶品质关系研究初报[J]. 茶叶科学技术,2007(4):6−8. [WANG Wenjian. Preliminary study on the relationship between Tieguanyin grafted on different rootstocks and tea quality[J]. Tea Science and Technology,2007(4):6−8.

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