Analysis on the Effects of Grafting on Tea Plant Metabolites Based on Targeted Metabolomics

CHA Fengguan; LIU Ying; GAO Jun; KONG Sheng; ZHAO Yiming; ZHOU Yongchen; ZUO Denghong; LÜ Caiyou; DING Haiqin; ZHENG Wenzhong

doi:10.13386/j.issn1002-0306.2021120126

Volume 43 Issue 21

Oct. 2022

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Science and Technology of Food Industry > 2022 > 43(21): 45-51. > DOI: 10.13386/j.issn1002-0306.2021120126

CHA Fengguan, LIU Ying, GAO Jun, et al. Analysis on the Effects of Grafting on Tea Plant Metabolites Based on Targeted Metabolomics[J]. Science and Technology of Food Industry, 2022, 43(21): 45−51. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120126.

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Analysis on the Effects of Grafting on Tea Plant Metabolites Based on Targeted Metabolomics

CHA Fengguan^{1, 2,},
LIU Ying¹,
GAO Jun^{1, 2, ,},
KONG Sheng^{1, 2},
ZHAO Yiming^{1, 2},
ZHOU Yongchen^{1, 2},
ZUO Denghong²,
LÜ Caiyou¹,
DING Haiqin¹,
ZHENG Wenzhong^{2, 3}

1.
College of Tea, Yunnan Agricultural University, Kunming 650100, China
2.
Cultivation Research Laboratory in Yunnan Modern Agricultural Tea Industry System, Kunming 650201, China
3.
Yunnan Pu'er Tea Tree Seed Farm, Puer 665000, China

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Received Date: December 12, 2021
Available Online: August 29, 2022

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Abstract

Abstract

In order to explore the difference of metabolites before and after tea tree grafting, tea tree grafting was carried out by cutting and grafting method using "Peach-shaped leaf" as the scion and "Short-grafted pekoe" as the rootstock. Substances in tea plants before and after grafting were detected by using broad-targeted metabolomics technology, and multivariate statistical methods such as principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to study the difference of metabolites before and after grafting. The results showed that a total of 804 metabolites were detected in tea samples before and after grafting, of which 105 metabolites were significantly different; Compared with that before grafting, 44 differential metabolites were significantly up-regulated in tea samples after grafting, and 61 differential metabolites were significantly down-regulated, and the number of differential metabolites down-regulated was greater than that up-regulated; In addition, the study on KEGG metabolic pathways revealed that these differential metabolites were mainly distributed in 20 metabolic pathways including purine metabolism, nicotinate and nicotinamide metabolism, glycerol phosphatide metabolism, flavonoid biosynthesis, flavonoid and flavonol biosynthesis, carbon sequestration by photosynthetic organisms, caffeine metabolism, and biosynthesis of secondary metabolites. Among them, there were 24 differential metabolites annotated by KEGG, which were dominated by flavone, nucleotides and their derivatives. In addition, most flavonoids in the pathways were significantly up-regulated, while nucleotides and their derivatives were significantly down-regulated. In conclusion, the content of metabolites of the variety "Peach-shaped leaf" is significantly changed after grafting, which can provide a theoretical reference for the processing and production of grafted tea leaves to a certain extent.
- tea plant grafting,
- broad-target metabolomics,
- differential metabolites,
- metabolic pathways,
- cutting and grafting method

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References

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