Inhibition Effects of Ethyl Acetate Extracts from <i>Inonotus obliquus</i> on Prion Replication

WANG Lin; YAO Qiang; YANG Wei; CHEN Jia; HU Chao; XIA Ying; CHEN Zhibao; CHEN Cao

doi:10.13386/j.issn1002-0306.2021120094

Volume 43 Issue 22

Nov. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(22): 23-30. > DOI: 10.13386/j.issn1002-0306.2021120094

WANG Lin, YAO Qiang, YANG Wei, et al. Inhibition Effects of Ethyl Acetate Extracts from Inonotus obliquus on Prion Replication[J]. Science and Technology of Food Industry, 2022, 43(22): 23−30. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120094.

Citation:

PDF (2914 KB)

Inhibition Effects of Ethyl Acetate Extracts from Inonotus obliquus on Prion Replication

WANG Lin^{1, 2, 3,},
YAO Qiang⁴,
YANG Wei^{3, 5},
CHEN Jia^{3, 5},
HU Chao^{3, 5},
XIA Ying³,
CHEN Zhibao^{1, 2, ,},
CHEN Cao^{3, 5, 6, 7, ,}

1.
College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163000, China
2.
Shenzhen Research Institute, Guangdong Ocean University, Shenzhen 518108, China
3.
National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
4.
China Animal Disease Provention and Control Center, Beijing 100125, China
5.
State Key Laboratory for Infectious Disease Control and Prevention, Beijing 102206, China
6.
Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China
7.
National Health Commission Key Laboratory for Medical Virology and Viral Diseases, Beijing 102206, China

More Information

Received Date: December 08, 2021
Available Online: September 20, 2022

Graphical Abstract

Abstract

Abstract

To investigate the effect and potential mechanism of ethyl acetate extracts from Inonotus obliquus in inhibiting the replicaton of prion, multiple methods were utilized. Using CCK-8 assay to evaluate the cytotoxicity of dichloromethane extracts, ethyl acetate extracts, n-butanol extracts and obtain the maximal concentrations of various extracts. Prion-infected cell model SMB-S15 were treated with three extracts at safe concentrations (0.1×2⁻¹⁴~0.1×2⁻⁸ mg/mL) and the inhibitory effects of different extracts on prion replication were analyzed by PrP-specific Western blots. The expression of reactive oxygen species, hydrogen peroxide and related antioxidant factors were measured in SMB-S15 cells treated with ethyl acetate extracts. The results showed that the maximum safe concentrations of dichloromethane extracts, ethyl acetate extracts and n-butanol extracts was 0.1×2⁻⁸ mg/mL. Prion replication was significantly inhibited in SMB-S15 cells treated with ethyl acetate extracts at this concentration of 0.1×2⁻⁸ mg/mL for 3 days (P<0.01). While there were no inhibitory effects on prion replication in SMB-S15 cells treated with dichloromethane extracts and n-butanol extracts. In addition, ethyl acetate extracts decreased the levels of reactive oxygen species and hydrogen peroxide, increased the expression level of HO-1, GCLC, total glutathione and enhanced the activity of SOD, with statistical difference. These data suggested that the ethyl acetate extracts from Inonotus obliquus could inhibit prion replication in vitro which might be related to the activation of Nrf2 signaling pathway.
- Inonotus obliquus,
- ethyl acetate extracts,
- prion replication,
- antioxidation,
- Nrf2 signaling pathway

FullText(HTML)

References (33)

References

[1]	PRUSINER S B. Nobel lecture: Prions[J]. Proc Natl Acad Sci,1998,95(23):13363−13383. doi: 10.1073/pnas.95.23.13363
[2]	HASIER E, VANESA V, JORGE M, et al. Prion-like disorders and transmissible spongiform encephalopathies: An overview of the mechanistic features that are shared by the various disease-related misfolded proteins[J]. Biochem Bioph Res Co,2017,483(4):1125−1136. doi: 10.1016/j.bbrc.2016.08.166
[3]	HANNAOUI S, SCHATZL H M, GILCH S, et al. Chronic wasting disease: Emerging prions and their potential risk[J]. PLoS Pathog,2017,13(11):e1006619. doi: 10.1371/journal.ppat.1006619
[4]	FORLONI G, ARTUSO V, ROITER I, et al. Therapy in prion diseases[J]. Curr Top Med Chem,2013,13(19):2465−2476. doi: 10.2174/15680266113136660173
[5]	SONG Y N, HUI J, KOU W, et al. Identification of Inonotus obliquus and analysis of antioxidation and antitumor activities of polysaccharides[J]. Curr Microbiol,2008,57(5):454−462. doi: 10.1007/s00284-008-9233-6
[6]	KIM H G, YOON D H, KIM C H, et al. Ethanol extract of Inonotus obliquus inhibits lipopolysaccharide-induced inflammation in RAW 264.7 macrophage cells[J]. J Med Food,2007,10(1):80−89. doi: 10.1089/jmf.2006.156
[7]	BALANDAYKIN M E, ZMITROVICH I V. Review on chaga medicinal mushroom, Inonotus obliquus (higher basidiomycetes): Realm of medicinal applications and approaches on estimating its resource potential[J]. Int J Med Mushrooms,2015,17(2):95−104. doi: 10.1615/IntJMedMushrooms.v17.i2.10
[8]	李敏, 罗益远, 宋世奇, 等. 不同产地桦褐孔菌无机元素的ICP-MS分析[J]. 北方园艺,2019(2):146−153. [LI Min, LUO Yiyuan, SONG Shiqi, et al. ICP-MS analysis of inorganic elements in Foramen betula from different habitats[J]. Northern Horticulture,2019(2):146−153.
[9]	CUI Y, KIM D S, PARK K C. Antioxidant effect of Inonotus obliquus[J]. J Ethnopharmacol,2005,96(1/2):79−85.
[10]	HU Y, TENG C Y, YU S M, et al. Inonotus obliquus polysaccharide regulates gut microbiota of chronic pancreatitis in mice[J]. AMB Express,2017,7(1):39. doi: 10.1186/s13568-017-0341-1
[11]	CHEN Y, HUANG Y, CUI Z, & LIU J. Purification, characterization and biological activity of a novel polysaccharide from Inonotus obliquus[J]. Int J Biol Macromol,2015,79:587−594. doi: 10.1016/j.ijbiomac.2015.05.016
[12]	NGUYET NGUYEN T M, LE H S, LE B V, et al. Anti-allergic effect of inotodiol, a lanostane triterpenoid from Chaga mushroom, via selective inhibition of mast cell function[J]. Int Immunopharmacol,2020,81(C):106244.
[13]	ZAHNG C J, GUO J Y, CHENG H, et al. Spatial structure and anti-fatigue of polysaccharide from Inonotus obliquus[J]. Int J Biol Macromol,2020,151:855−860. doi: 10.1016/j.ijbiomac.2020.02.147
[14]	BAEK J, ROH H S, BAEK K H, et al. Bioactivity-based analysis and chemical characterization of cytotoxic constituents from Chaga mushroom (Inonotus obliquus) that induce apoptosis in human lung adenocarcinoma cells[J]. J Ethnopharmacol,2018,224:63−75. doi: 10.1016/j.jep.2018.05.025
[15]	STANISZEWSKA J, SZYMAŃSKI M, IGNATOWICZ E. Antitumor and immunomodulatory activity of Inonotus obliquus[J]. Herba Polonica,2017,63(2):48−58. doi: 10.1515/hepo-2017-0013
[16]	SONG F Q, LIU Y, KONG X S, et al. Progress on understanding the anticancer mechanisms of medicinal mushroom: Inonotus obliquus[J]. Asian Pacific Journal of Cancer Prevention,2013,14(3):1571−1578. doi: 10.7314/APJCP.2013.14.3.1571
[17]	杨微, 陈志宝, 陈操, 等. 桦褐孔菌乙醇粗提物对朊病毒复制的抑制作用[J]. 现代食品科技,2021,37(7):8−13,7. [YANG Wei, CHEN Zhibao, CHEN Cao, et al. Inhibition on prion replication by ethanol crude extracts from Inonotus obliquus[J]. Modern Food Science and Technology,2021,37(7):8−13,7. doi: 10.13982/j.mfst.1673-9078.2021.7.1126
[18]	师苑, 朱红薇, 张运洋, 等. 桦褐孔菌不同提取物提取工艺及生物活性研究[J]. 广州化工,2018,46(7):67−69. [SHI Yuan, ZHU Hongwei, ZAHNG Yunyang, et al. Study on extraction technology and biological activity of different extracts from Inonotus obliquus[J]. Guangdong Chemical Industry,2018,46(7):67−69. doi: 10.3969/j.issn.1001-9677.2018.07.022
[19]	HUANG K K, RAMNARAYANAN K, ZHU F, et al. Genomic and epigenomic profiling of high-risk intestinal metaplasia reveals molecular determinants of progression to gastric cancer[J]. Cancer Cell,2018,33(1):137−150. doi: 10.1016/j.ccell.2017.11.018
[20]	ZHOU D H, WANG J, XIAO K, et al. Stilbene compounds inhibit the replications of various strains of prions in the levels of cell culture, PMCA and RT-QuIC possibly via molecular binding[J]. ACS Chemical Neuroscience,2020,11(14):2117−2128. doi: 10.1021/acschemneuro.0c00218
[21]	YIN Y, WANG Z, CHENG D, et al. The ATP-binding protein FgArb1 is essential for penetration, infectious and normal growth of Fusarium graminearum[J]. New Phytologist,2018,219(4):1447−1466. doi: 10.1111/nph.15261
[22]	LUO T W, LIU G, LONG M F, et al. Treatment of cadmium-induced renal oxidative damage in rats by administration of alpha-lipoic acid[J]. Environ Sci Pollut Res Int,2017,24(2):1832−1844. doi: 10.1007/s11356-016-7953-x
[23]	JIA Y, WANG HD, WANG Q, et al. GSH depletion and consequent AKT inhibition contribute tothe Nrf2 knockdown-induced decrease in proliferation in glioblastoma U251 cells[J]. Oncology Reports,2017,37(4):2252−2260. doi: 10.3892/or.2017.5467
[24]	BROCKES J P. Topics in prion cell biology[J]. Curr Opin Neurobiol,1999,9(5):571−577. doi: 10.1016/S0959-4388(99)00016-1
[25]	YOKOYAMA T, MOHRI S. Prion diseases and emerging prion diseases[J]. Current Medicinal Chemistry,2008,15(9):912−916. doi: 10.2174/092986708783955437
[26]	刘向辉, 谢人明, 陈琳, 等. 桦褐孔菌不同提取物小鼠急性毒性实验研究[J]. 中国药物警戒,2013,10(7):390−392,395. [LIU Xianghui, XIE Renming, CHEN Lin, et al. Experimental study on acute toxicity of different extracts of Inonotus obliquus in mice[J]. Chinese Journal of Pharmacovigilance,2013,10(7):390−392,395. doi: 10.3969/j.issn.1672-8629.2013.07.002
[27]	阿达莱提·买买提明, 武月章, 谢姆斯耶·吾拉音, 等. 线粒体靶向抗氧化剂Mitoquinone和积雪草酸对朊病毒感染的神经细胞的治疗作用研究[J]. 病毒学报,2019,35(2):247−254. [MAIMAITIMING Adalaiti, WU Yuezhang, WULAYIN Xiemusiye, et al. The therapeutic role of mitochondria-targeted antioxidants mitoquinone and asiatic acid in scrapie infected cell line SMB-S15[J]. Chinese Journal of Virology,2019,35(2):247−254. doi: 10.13242/j.cnki.bingduxuebao.003522
[28]	HAN Y Q, NAN S J, FAN J, et al. Inonotus obliquus polysaccharides protect against Alzheimer's disease by regulating Nrf2 signaling and exerting antioxidative and antiapoptotic effects[J]. Int J Biol Macromol,2019,131:769−778. doi: 10.1016/j.ijbiomac.2019.03.033
[29]	YUN J S, PAHK J W, LEE J S, et al. Inonotus obliquus protects against oxidative stress-induced apoptosis and premature senescence[J]. Mol Cells,2011,31(5):423−429. doi: 10.1007/s10059-011-0256-7
[30]	CASHMAN N R, CAUGHEY B. Prion diseases-close to effective therapy?[J]. Nat Rev Drug Discov,2004,3(10):874−884. doi: 10.1038/nrd1525
[31]	AGUZZI A, LAKKARAJU A K K, FRONTZEK K. Toward therapy of human prion diseases[J]. Annu Rev Pharmacol Toxicol,2018,58:331−351. doi: 10.1146/annurev-pharmtox-010617-052745
[32]	LI Y, ZHOU Y, WU J, et al. Phelligridin D from Inonotus obliquus attenuates oxidative stress and accumulation of ECM in mesangial cells under high glucose via activating Nrf2[J]. Chin J Nat Medicines,2021,75(4):1021−1029.
[33]	XU J, GAN S, LI J, et al. Garcinia xanthochymus extract protects PC12 cells from H₂O₂-induced apoptosis through modulation of PI3K/AKT and NRF2/HO-1 pathways[J]. Chin J Nat Medicines,2017,15(11):825−833. doi: 10.1016/S1875-5364(18)30016-5