Characterization of Key Aroma Compounds of Apple Slices Dried by Hot-air at Different Temperatures by GC-MS and Electronic Nose

LI Jiaxin; WU Xinye; BI Jinfeng; GOU Min

doi:10.13386/j.issn1002-0306.2021120071

Volume 43 Issue 18

Sep. 2022

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Science and Technology of Food Industry > 2022 > 43(18): 272-282. > DOI: 10.13386/j.issn1002-0306.2021120071

LI Jiaxin, WU Xinye, BI Jinfeng, et al. Characterization of Key Aroma Compounds of Apple Slices Dried by Hot-air at Different Temperatures by GC-MS and Electronic Nose[J]. Science and Technology of Food Industry, 2022, 43(18): 272−282. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120071.

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Characterization of Key Aroma Compounds of Apple Slices Dried by Hot-air at Different Temperatures by GC-MS and Electronic Nose

LI Jiaxin^1,,
WU Xinye²,
BI Jinfeng^{1, 2, ,},
GOU Min²

1.
School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
2.
Institute of Food Science and Technology, CAAS, Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193, China

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Received Date: December 06, 2021
Available Online: July 11, 2022

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Abstract

Abstract

To clarify the key aroma compounds of Fuji apples and the influence of drying temperatures, the volatile compounds of Fuji apple slices dried by hot-air at 50, 60, 70 and 80 ℃ were characterized by head space solid-phase micro-extraction gas chromatography-mass spectrometer (HS-SPME-GC-MS) combined with electronic nose. The results showed that 64 volatile compounds were identified in fresh and four kinds of dried apple samples, including 23 esters, 18 alcohols, 8 aldehydes, 2 alkenes, 2 ketones, 3 lactones, 1 acid, 3 sulfur-containing compounds and 4 heterocyclic compounds. The differences between types and contents of volatile compounds in different samples were great. The content of aroma compounds in fresh samples was 397.059 mg/kg. Total volatile compounds contents in four hot-air dried samples from high to low were 64.189 mg/kg at 50 ℃, 57.703 mg/kg at 80 ℃, 32.124 mg/kg at 70 ℃ and 32.020 mg/kg at 60 ℃, respectively. Odor-active value (OAV) analysis showed that there were 8 key aroma compounds both in fresh apple sample and hot-air dried apple samples. They were α-farnesene (12746.11~1597.75), hexyl 2-methylbutyrate (755.62~6.90), hexanol (2988.00~168.54), 1-octene-3-ol (53.12~12.08), nonanal (1534.99~47.36), trans-2-nonenal (1202.98~189.38), linalool (1264.30~212.75) and 6-methy-5-hepten-2-one (11.27~3.90). The key aroma compounds identified in hot-air dried samples included 2-methyl-1-butanol (32.26~7.16), 3-methyl-4-heptanol (14.39~6.90), phenylethanol (11.11~4.67), octanal (211.25~84.36), 3-hydroxy-2-butanone (64.57~21.86), 3-methylthiopropanol (13.52~5.88) and 2-pentylfuran (26.44~14.88). Electronic nose analysis showed that there were differences in aroma profiles between fresh apple and apple slices dried by hot-air at different temperatures. The fresh samples and dried apple slices could be effectively distinguished by principle component analysis (PCA). Considering aroma and energy consumption, 80 ℃ hot air dehydration apple slices had better characteristic aroma than 50 ℃ hot air treatments. Therefore, 80 ℃ hot air drying was the optimal drying condition.

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References

[1]	王崧百. 苹果脆片产业发展研究[J]. 食品安全导刊,2021(26):166−168. [WANG S B. Study on the development of apple chips industry[J]. China Food Safety Magazine,2021(26):166−168. doi: 10.16043/j.cnki.cfs.2021.26.111 WANG S B. Study on the development of apple chips industry[J]. China Food Safety Magazine, 2021(26): 166-168. doi: 10.16043/j.cnki.cfs.2021.26.111
[2]	楚倩倩, 任广跃, 段续, 等. 过热蒸汽和热风干燥在食品领域中的应用对比[J/OL]. 食品与发酵工业: 1−12[2022-01-08] CHU Q Q, REN G Y, DUAN X, et al. Application comparison of superheated steam and hot-air drying in the food field[J/OL]. Food and Fermentation Industries: 1−12[2022-01-08].
[3]	莫一凡, 姚凌云, 冯涛, 等. 水果干风味物质及干燥方式的影响研究[J]. 中国果菜,2020,40(6):23−28,40. [MO Y F, YAO L Y, FENG T, et al. Study on flavor substances of dried fruits and the effects of drying methods[J]. China Fruit & Vegetable,2020,40(6):23−28,40. 6): 23-28, 40. MO Y F, YAO L Y, FENG T, et al. Study on flavor substances of dried fruits and the effects of drying methods[J]. China Fruit & Vegetable, 2020, 40(6): 23-28, 40.
[4]	郭卫芸, 李光辉, 张珍珍, 等. 香椿中特征性香气成分定量分析及其在热风干燥过程中的变化规律[J]. 食品研究与开发,2021,42(3):14−19. [GUO W Y, LI G H, ZHANG Z Z, et al. Quantitative analysis of Toona sinensis aroma components and viariation rule during hot air dry process[J]. Food Research and Development,2021,42(3):14−19. GUO W Y, LI G H, ZHANG Z Z, et al. Quantitative analysis of Toona sinensis aroma components and viariation rule during hot air dry process[J]. Food Research and Development, 2021, 42(3): 14-19.
[5]	DIMICK P S, HOSKIN J C, ACREE T E. Review of apple flavor-State of the art[J]. C R C Critical Reviews in Food Science & Nutrition,1983,18:387−409.
[6]	REIS S, ROCHA S, BARROS A, et al. Establishment of the volatile profile of ‘Bravo de Esmolfe’ apple variety and identification of varietal markers[J]. Food Chemistry,2009,113(2):513−521. doi: 10.1016/j.foodchem.2008.07.093
[7]	石芬, 徐军, 姜宗伯, 等. HS-SPME-GC-MS结合多元统计分析初榨椰子油常温储藏过程中挥发性风味成分[J]. 食品工业科技, 2022, 43(10):314-322. SHI F, XU J, JIANG Z B, et al. Analysis of volatile flavor components of virgin coconut oil during normal temperature storage based on HS-SPME-GC-MS and multivariate statistical analysis[J]. Science and Technology of Food Industry, 2022, 43(10):314-322.
[8]	王铁儒, 郭丽, 马曼, 等. SPME-GC-MS与电子鼻结合分析不同酵母混菌发酵猕猴桃酒的挥发性香气物质[J]. 食品工业科技,2021,42(16):119−128. [WANG T R, GUO L, MA M, et al. Analysis of volatile aroma compounds in kiwi wine co-fermentation with different yeasts by SPME-GC-MS combined with electronic nose[J]. Science and Technology of Food Industry,2021,42(16):119−128. doi: 10.13386/j.issn1002-0306.2021010133 WANG T R, GUO L, MA M, et al. Analysis of volatile aroma compounds in kiwi wine co-fermentation with different yeasts by SPME-GC-MS combined with electronic nose[J]. Science and Technology of Food Industry, 2021, 42(16): 119-128. doi: 10.13386/j.issn1002-0306.2021010133
[9]	TIMOUMI S, MIHOUBI D, ZAGROUBA F. Shrinkage, vitamin C degradation and aroma losses during infra-red drying of apple slices[J]. LWT-Food Science and Technology,2007,40:1648−1654. doi: 10.1016/j.lwt.2006.11.008
[10]	KROKIDA M K, PHILIPPOPOULOS C. Volatility of apples during air and freeze drying[J]. Journal of Food Engineering,2006,73(2):135−141. doi: 10.1016/j.jfoodeng.2005.01.012
[11]	SONG J, CHEN Q, BI J, et al. GC/MS coupled with MOS e-nose and flash GC e-nose for volatile characterization of Chinese jujubes as affected by different drying methods[J]. Food Chemistry,2020,331:127201. doi: 10.1016/j.foodchem.2020.127201
[12]	曹有芳, 刘丹, 徐俊南, 等. 基于电子鼻和气相色谱-质谱联用技术分析不同品种苹果酒香气物质[J]. 中国酿造,2020,39(2):182−188. [CAO Y F, LIU D, XV J N, et al. Analysis of aroma substances in apple wines brewed with different varieties of apple by electronic nose combined with GC-MS[J]. China Brewing,2020,39(2):182−188. doi: 10.11882/j.issn.0254-5071.2020.02.034 CAO Y F, LIU D, XV J N, et al. Analysis of aroma substances in apple wines brewed with different varieties of apple by electronic nose combined with GC-MS[J]. China Brewing, 2020, 39(2): 182-188. doi: 10.11882/j.issn.0254-5071.2020.02.034
[13]	农业部农产品加工局. NY/T 2779-2015 苹果脆片[S]. 北京: 中国人民共和国农业部, 2015. Agricultural Products Processing Bureau of Ministry of Agriculture. NY/T 2779-2015 Apple crisp chips[S]. Beijing: The Ministry of Agriculture of the People’s Republic of China, 2015.
[14]	曾辉. 不同品种苹果特征香气的表征与识别[D]. 长沙: 湖南农业大学, 2016. ZENG H. Research on representation and recognition of characteristic aroma of different apple cultivars[D]. Changsha: Hunan Agricultural University, 2016.
[15]	于怀龙, 马永昆, 张荣, 等. 不同品种桑椹香气成分的主成分分析[J]. 食品工业科技,2016,37(10):62−66,71. [YU H L, MA Y K, ZHANG R, et al. Principal component analysis of aroma components in mulberry from different varieties[J]. Science and Technology of Food Industry,2016,37(10):62−66,71. doi: 10.13386/j.issn1002-0306.2016.10.003 YU H L, MA Y K, ZHANG R, et al. Principal component analysis of aroma components in mulberry from different varieties[J]. Science and Technology of Food Industry, 2016, 37(10): 62-66, 71. doi: 10.13386/j.issn1002-0306.2016.10.003
[16]	万鹏, 梁国平, 马丽娟, 等. 19个苹果品种果实香气成分的GC-MS分析[J]. 食品工业科技,2019,40(14):227−232. [WAN P, LIANG G P, MA L J, et al. GC-MS analysis of fruit aroma components in 19 apple varieties[J]. Science and Technology of Food Industry,2019,40(14):227−232. WAN P, LIANG G P, MA L J, et al. GC-MS analysis of fruit aroma components in 19 apple varieties[J]. Science and Technology of Food Industry, 2019, 40(14): 227-232.
[17]	ZHU J, CHEN F, WANG L, et al. Characterization of the key aroma volatile compounds in cranberry (Vaccinium macrocarpon Ait. ) using gas chromatography–olfactometry (GC-O) and odor activity value (OAV)[J]. Journal of Agricultural and Food Chemistry,2016,64:4990−4999. doi: 10.1021/acs.jafc.6b01150
[18]	DIXON J, HEWETT E W. Factors affecting apple aroma/flavour volatile concentration: A review[J]. New Zealand Journal of Crop and Horticultural Science,2000,28(3):155−173. doi: 10.1080/01140671.2000.9514136
[19]	GIRARD B, LAU O L. Effect of maturity and storage on quality and volatile production of “Jonagold” apples[J]. Food Research International,1995,28(5):465−471. doi: 10.1016/0963-9969(96)81393-7
[20]	APREA E, COROLLARO M L, BETTA E, et al. Sensory and instrumental profiling of 18 apple cultivars to investigate the relation between perceived quality and odour and flavour[J]. Food Research International,2012,49(2):677−686. doi: 10.1016/j.foodres.2012.09.023
[21]	ZHANG J, CAO Z, PEI P, et al. Volatile flavour components and the mechanisms underlying their production in golden pompano (Trachinotus blochii) fillets subjected to different drying methods: A comparative study using an electronic nose, an electronic tongue and SDE-GC-MS[J]. Food Research International,2019,123:217−225. doi: 10.1016/j.foodres.2019.04.069
[22]	张志兵, 连琛, 詹瑞玲, 等. 气相色谱-质谱联用法分析不同品种苹果酿造蒸馏酒中香气成分及特征[J]. 中国酿造,2021,40(9):191−195. [ZHANG Z B, LIAN S, ZHAN R L, et al. Analysis of aroma components and characteristics in apple distillations brewed with different varieties of apple by GC-MS[J]. China Brewing,2021,40(9):191−195. doi: 10.11882/j.issn.0254-5071.2021.09.034 ZHANG Z B, LIAN S, ZHAN R L, et al. Analysis of aroma components and characteristics in apple distillations brewed with different varieties of apple by GC-MS[J]. China Brewing, 2021, 40(9): 191-195. doi: 10.11882/j.issn.0254-5071.2021.09.034
[23]	赵志聪, 白启正, 连琛, 等. 苹果白兰地原酒蒸馏过程中挥发性香气成分的变化[J]. 现代食品,2021(15):80−85. [ZHAO Z C, BAI Q Z, LIAN C, et al. Changes of volatile aroma components during distillation of apple brandy[J]. Modern Food,2021(15):80−85. doi: 10.16736/j.cnki.cn41-1434/ts.2021.15.021 ZHAO Z C, BAI Q Z, LIAN C, et al. Changes of volatile aroma components during distillation of apple brandy[J]. Modern Food, 2021(15): 80-85. doi: 10.16736/j.cnki.cn41-1434/ts.2021.15.021
[24]	裴鹏正, 贠建民, 贾琦, 等. 软儿梨果酒发酵过程中挥发性风味物质变化分析[J]. 生物技术进展,2021,11(6):758−769. [PEI P Z, YUN J M, JIA Q, et al. Analysis on changes of volatile flavor compounds in Zuan’er pear wine during fermentation[J]. Current Biotechnology,2021,11(6):758−769. doi: 10.19586/j.2095-2341.2021.0086 PEI P Z, YUN J M, JIA Q, et al. Analysis on changes of volatile flavor compounds in Zuan’er pear wine during fermentation[J]. Current Biotechnology, 2021, 11(6): 758-769. doi: 10.19586/j.2095-2341.2021.0086
[25]	CHUNG H Y, YUNG I, MA W, et al. Analysis of volatile components in frozen and dried scallops (Patinopecten yessoensis) by gas chromatography/mass spectrometry[J]. Food Research International,2002,35(1):43−53. doi: 10.1016/S0963-9969(01)00107-7
[26]	LIU H, WANG Z, ZHANG D, et al. Characterization of key aroma compounds in Beijing Roasted Duck by gas chromatography-olfactometry-mass spectrometry, odor activity values and aroma recombination experiments[J]. Journal of Agricultural and Food Chemistry, 2019, 67(20):5847−5856.
[27]	TAIRU A O, HOFMANN T, SCHIEBERLE P. Characterization of the key aroma compounds in dried fruits of the West African peppertree Xylopia aethiopica (Dunal) A. Rich (Annonaceae) using aroma extract dilution analysis[J]. Journal of Agricultural & Food Chemistry,1999,47(8):3285−3287.
[28]	JORRY, DHARMAWAN, STEFAN, et al. Evaluation of aroma-active compounds in pontianak orange peel oil (Citrus nobilis Lour. Var. microcarpa Hassk.) by gas chromatography olfactometry, aroma reconstitution, and omission test[J]. Journal of Agricultural and Food Chemistry,2009,57(1):239−244. doi: 10.1021/jf801070r
[29]	VANOLI M, VISAI C, RIZZOLO A. The influence of harvest date on the volatile composition of 'Starkspur Golden' apples[J]. Postharvest Biology and Technology,1995,6(3):225−234.
[30]	VARMING C, ANDERSEN M L. Influence of thermal treatment on black currant (Ribes nigrum L.) juice aroma[J]. Journal of Agricultural & Food Chemistry,2004,52(25):7628−7636.
[31]	BERDAGUE J L, MONTEIL P, MONTEL M C, et al. Talon effects of starter cultures on the formation of flavour compounds in dry sausage[J]. Meat Science,1993,35:275−287. doi: 10.1016/0309-1740(93)90033-E
[32]	BARBIERI G, BOLZONI L, PAROLARI G, et al. Flavor compounds of dry-cured ham[J]. Journal of Agricultural & Food Chemistry,1992,40(12):2389−2394.
[33]	PELLEGRINO, CONTE, GENNARO, et al. Comparing different processing methods in apple slice drying. Part 2 solid-state fast field cycling 1H-NMR relaxation properties, shrinkage and changes in volatile compounds[J]. Biosystems Engineering,2019,188:345−354. doi: 10.1016/j.biosystemseng.2019.10.020
[34]	MISHARINA T A, MUHUTDINOVA S M, ZHARIKOVA G G, et al. Formation of flavor of dry champignons (Agaricus bisporus L.)[J]. Applied Biochemistry & Microbiology,2010,46(1):108−113.
[35]	VALAPPIL Z A, FAN X, ZHANG H Q, et al. Impact of thermal and nonthermal processing technologies on unfermented apple cider aroma volatiles[J]. J Agric Food Chem,2009,57(3):924−929. doi: 10.1021/jf803142d
[36]	MOREIRA N, MENDES F, PEREIRA O, et al. Volatile sulphur compounds in wines related to yeast metabolism and nitrogen composition of grape musts[J]. Analytica Chimica Acta,2002,458(1):157−167. doi: 10.1016/S0003-2670(01)01618-X
[37]	METHVEN L, MARIA. Influence of sulfur amino acids on the volatile and nonvolatile components of cooked salmon (Salmo salar)[J]. Journal of Agricultural and Food Chemistry,2007,55(4):1427−1436. doi: 10.1021/jf0625611
[38]	RODRIGO O S, CONSUELO D M, SOLEDAD P C, et al. Viability of pre-treatment drying methods on mango peel by-products to preserve flavouring active compounds for its revalorisation-ScienceDirect[J]. Journal of Food Engineering,2020,279:109953. doi: 10.1016/j.jfoodeng.2020.109953
[39]	GOKMEN V, MORALES F. Processing contaminants: Hydroxymethylfurfural[J]. Encyclopedia of Food Safety,2014,2:404−408.
[40]	RANNOU C, LAROQUE D, RENAULT E, et al. Mitigation strategies of acrylamide, furans, heterocyclic amines and browning during the maillard reaction in foods[J]. Food Research International,2016,90(PT.3):154−176.
[41]	AMEUR L A, REGA B, GIAMPAOLI P, et al. The fate of furfurals and other volatile markers during the baking process of a model cookie[J]. Food Chemistry,2008,111(3):758−763. doi: 10.1016/j.foodchem.2007.12.062
[42]	里奥·范海默特. 化合物香味阈值汇编[M]. 北京: 科学出版社, 2015. VAN GEMERT J L. Compilations of flavour threshold values in water and other media[M]. Beijing: Science Press, 2015.
[43]	VCF online by BeWiDo BV Inc. Volatile compounds in food[DB/OL]. [2021-11-30].https://www.vcf-online.nl/VcfHome.cfm.
[44]	Gas chromatography-olfactometry (GCO) of natural products sponsored by DATU Inc. Flavornet and human odor space[DB/OL]. [2021-11-30]. http://www.flavornet.org/flavornet.html.

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