Preparation and Evaluation of Antioxidant Activity of<i> Haematococcus pluvialis</i> Hydrolysate

HE Wanshi; XU Jin; CAO Yong; WANG Zhongming; LIU Xiaojuan

doi:10.13386/j.issn1002-0306.2021120047

Volume 43 Issue 17

Aug. 2022

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Science and Technology of Food Industry > 2022 > 43(17): 248-257. > DOI: 10.13386/j.issn1002-0306.2021120047

HE Wanshi, XU Jin, CAO Yong, et al. Preparation and Evaluation of Antioxidant Activity of Haematococcus pluvialis Hydrolysate[J]. Science and Technology of Food Industry, 2022, 43(17): 248−257. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120047.

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Preparation and Evaluation of Antioxidant Activity of Haematococcus pluvialis Hydrolysate

1.
Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China
2.
College of Food Science, South China Agricultural University, Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, Guangzhou 510642, China

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Received Date: December 05, 2021
Available Online: July 04, 2022

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Abstract

Abstract

In order to make high value use of the algal residue, this paper presented a study on the preparation of antioxidant enzymatic hydrolysate from Haematococcus pluvialis residue. Single factor investigation and the response surface methodology (RSM) were used to optimize the hydrolysis process of H. pluvialis residue, including substrate concentration, dosage of proteases, dosage of proteases, temperature, pH and time. The resulting hydrolysates were comparatively evaluated for their ABTS scavenging capacity and degree of hydrolysis. Subsequently, the antioxidant ability and amino acid composition of the optimal hydrolysate were investigated. The results showed that under the optimal hydrolysis conditions: Substrate concentration of 9% (w:v), dosage of proteases of 0.7% (w:w), temperature of 40 ℃, pH11.5 and time of 3 h, the highest ABTS scavenging activity with IC₅₀ of 481.574 μg/mL and FRAP value of 19.641 μmol FeSO₄·7H₂O /g (concentration of 1000 μg/mL) were obtained. Antioxidant enzymatic hydrolysate with 34.4% essential amino acid not only contained high nutritional value but also involved many hydrophobic amino acids closely related to antioxidant activity. This research provides a reference for high value-added applications of H. pluvialis.
- Haematococcus pluvialis,
- enzymatic hydrolysates,
- antioxidant ability,
- response surface methodology (RSM),
- amino acid composition

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[1]	SHAH M M R, LIANG Y M, CHENG J J, et al. Astaxanthin-producing green microalga Haematococcus pluvialis: From single cellto high value commercia products[J]. Frontiers in Plant Science,2016,7(531):1−28.
[2]	FANG N, WANG C K, LIU X F, et al. De novo synthesis of astaxanthin: From organisms to genes[J]. Trends in Food Science & Technology,2019,92:162−171.
[3]	刘涵, 张苗, 刘晓娟, 等. 雨生红球藻多糖的分离纯化和免疫活性组分鉴定[J]. 食品科学,2019,40(2):52−58. [LIU H, ZHANG M, LIU X J. Isolation, purification and identification of immunologically active polysaccharides from Haematococcus pluvialis[J]. Food Science,2019,40(2):52−58. doi: 10.7506/spkx1002-6630-20171116-208 LIU H, ZHANG M, LIU X J. Isolation, purification and identification of immunologically active polysaccharides from Haematococcus pluvialis[J]. Food Science, 2019, 40(2): 52-58. doi: 10.7506/spkx1002-6630-20171116-208
[4]	张苗. 雨生红球藻多糖的分离纯化、结构鉴定及增强免疫和延缓衰老活性研究[D]. 广州: 华南农业大学, 2017. ZHANG M. Separation, structural analysis of Haematococcus pluvialis polysaccharide and its function on immune and anti-aging activity[D]. Guangzhou: South China Agricultural University, 2017.
[5]	ZHU Y P, ZHAO X Y, ZHANG X W, et al. Amino acid, structure and antioxidant properties of Haematococcus pluvialis protein hydrolysates produced by different proteases[J]. International Journal of Food Science and Technology,2021,56(1):185−195. doi: 10.1111/ijfs.14618
[6]	ZHU Y P, ZHAO X Y, ZHANG X W, et al. Extraction, structural and functional properties of Haematococcus pluvialis protein after pigment removal[J]. International Journal of Biological Macromolecules,2019,140:1073−1083. doi: 10.1016/j.ijbiomac.2019.08.209
[7]	邱月. 雨生红球藻中藻蛋白制备工艺及其蛋白的结构与功能性研究[D]. 济南: 济南大学, 2020. QIU Y. Study on algae protein preparation process and protein structure and function in Haematococcus pluvialis[D]. Jinan: University of Jinan, 2020.
[8]	CAPORGNO M P, MATHYS A. Trends in microalgae incorporation into innovative food products with potential health benefits[J]. Frontiers in Nutrition,2018,5(58):1−10.
[9]	AMORIM M L, SOARES J, COIMBRA J S D R, et al. Microalgae proteins: Production, separation, isolation, quantification, and application in food and feed[J]. Critical Reviews in Food Science and Nutrition,2021,61(12):1976−2002. doi: 10.1080/10408398.2020.1768046
[10]	BERTSCH P, BOECKER L, MATHYS A, et al. Proteins from microalgae for the stabilization of fluid interfaces, emulsions, and foams[J]. Trends in Food Science & Technology,2021,108:326−342.
[11]	ANDREEVA A, BUDENKOVA E, BABICH O, et al. Production, purification, and study of the amino acid composition of microalgae proteins[J]. Molecules,2021,26:27679.
[12]	NORZAGARAY V C D, VALDEZ O A, SHELTON L M, et al. Residual biomasses and protein hydrolysates of three green microalgae species exhibit antioxidant and anti-aging activity[J]. Journal of Applied Phycology,2017,29(1):189−198. doi: 10.1007/s10811-016-0938-9
[13]	BARKIA I, AL H L, ABDUL H A, et al. Indigenous marine diatoms as novel sources of bioactive peptides with antihypertensive and antioxidant properties[J]. International Journal of Food Science & Technology,2019,54(5):1514−1522.
[14]	THANH S V, RYU B M, KIM S K. Purification of novel anti-inflammatory peptides from enzymatic hydrolysate of the edible microalgal Spirulina maxima[J]. Journal of Functional Foods,2013,5(3):1336−1346. doi: 10.1016/j.jff.2013.05.001
[15]	WANG X Q, ZHANG X W. Separation, antitumor activities, and encapsulation of polypeptide from Chlorella pyrenoidosa[J]. Biotechnology Progress,2013,29(3):681−687. doi: 10.1002/btpr.1725
[16]	ROJAS V, RIVAS L, CARDENAS C, et al. Cyanobacteria and eukaryotic microalgae as emerging sources of antibacterial peptides[J]. Molecules,2020,25:580424.
[17]	郑淳坚, 郑虹君, 陈容, 等. 酶解制备小球藻多肽及其抗氧化与稳定性研究[J]. 中国食品添加剂,2021,32(6):1−6. [ZHENG C J, ZHENG H J, CHEN R, et al. Preparation of Chlorella peptide by enzymatic hydrolysis and study of its antioxidant activity and stability[J]. China Food Additives,2021,32(6):1−6. doi: 10.19804/j.issn1006-2513.2021.06.001 ZHENG C J, ZHENG H J, CHEN R, et al. Preparation of Chlorella peptide by enzymatic hydrolysis and study of its antioxidant activity and stability[J]. China Food Additives, 2021, 32(6): 1-6. doi: 10.19804/j.issn1006-2513.2021.06.001
[18]	王文强. 小球藻酶解多肽的制备及抗氧化功能研究[D]. 福建: 福建师范大学, 2018. WANG W Q. Study on the preparation and antioxidant function of enzymatic hydrolyzed peptides of Chlorella[D]. Fujian: Fujian Normal University, 2018.
[19]	王爽, 张高帆, 陈欣悦, 等. 小球藻蛋白的制备及其水解肽的抗氧化研究[J]. 中国食品学报,2017,17(2):92−100. [WANG S, ZHANG G F, CHEN X Y, et al. Preparation of Chlorella protein and antioxidant study of its hydrolyzed peptides[J]. Journal of Chinese Institute of Food Science and Technology,2017,17(2):92−100. doi: 10.16429/j.1009-7848.2017.02.013 WANG S, ZHANG G F, CHEN X Y, et al. Preparation of Chlorella protein and antioxidant study of its hydrolyzed peptides[J]. Journal of Chinese Institute of Food Science and Technology, 2017, 17(2): 92-100. doi: 10.16429/j.1009-7848.2017.02.013
[20]	郝晓丽, 张霞, 李磊, 等. 驼乳与牛乳乳清蛋白酶解工艺优化及其产物抗氧化能力分析[J]. 食品工业科技,2020,41(13):187−194. [HAO X L, ZHANG X, LI L, et al. Optimization of enzymatic hydrolysis of camel milk and milk whey protein and analysis of its antioxidant capacity[J]. Science and Technology of Food Industry,2020,41(13):187−194. doi: 10.13386/j.issn1002-0306.2020.13.030 HAO X L, ZHANG X, LI L, et al. Optimization of enzymatic hydrolysis of camel milk and milk whey protein and analysis of its antioxidant capacity[J]. Science and Technology of Food Industry, 2020, 41(13): 187-194. doi: 10.13386/j.issn1002-0306.2020.13.030
[21]	宁诗文, 崔珊珊, 尚宏丽. 响应面法优化大黄花鱼肉蛋白水解工艺及多肽抗氧化性研究[J]. 食品工业科技,2020,41(13):219−226. [NING S W, CUI S S, SHANG H L. Optimization of the protein hydrolysis process by response surface method and the antioxidant properties of polypeptides in large yellow croaker[J]. Science and Technology of Food Industry,2020,41(13):219−226. doi: 10.13386/j.issn1002-0306.2020.13.035 NING S W, CUI S S, SHANG H L. Optimization of the protein hydrolysis process by response surface method and the antioxidant properties of polypeptides in large yellow croaker[J]. Science and Technology of Food Industry, 2020, 41(13): 219-226. doi: 10.13386/j.issn1002-0306.2020.13.035
[22]	NIELSEN P M, PETERSEN D, DAMBMANN C. Improved method for determining food protein degree of hydrolysis[J]. Journal of Food Science,2001,66(5):642−646. doi: 10.1111/j.1365-2621.2001.tb04614.x
[23]	BA F, URSU A V, LAROCHE C, et al. Haematococcus pluvialis soluble proteins: Extraction, characterization, concentration/fractionation and emulsifying properties[J]. Bioresource Technology,2016,200:147−152. doi: 10.1016/j.biortech.2015.10.012
[24]	马梦娇. 中华鳖肉抗氧化肽的制备及其抗衰老功能研究[D]. 无锡: 江南大学, 2020. MA M J. Preparation of antioxidant peptides from Chinese soft-shelled turtle and its anti-aging activity[D]. Wuxi: Jiangnan University, 2020.
[25]	TACIAS P V G, MORELLON S R, SIAR E H, et al. Use of alcalase in the production of bioactive peptides: A review[J]. International Journal of Biological Macromolecules,2020,165:2143−2196. doi: 10.1016/j.ijbiomac.2020.10.060
[26]	马艳芳. 螺旋藻肽的制备及其抗氧化性研究[D]. 泰安: 山东农业大学, 2016. MA Y F. Preparation and functional properties of Spirulina antioxidant peptides[D]. Taian: Shandong Agricultural University, 2016.
[27]	武琼. 裂壶藻蛋白源抗氧化肽的制备分离及稳定性研究[D]. 青岛: 中国海洋大学, 2015. WU Q. Study on the preparation, purification and stability of Schizochytrium limacinum antioxidant peptide[D]. Qingdao: Ocean University of China, 2015.
[28]	KRISTINSSON H G, RASCO B A. Fish protein hydrolysates: Production, biochemical, and functional properties[J]. Critical Reviews in Food Science and Nutrition,2000,40(1):43−81. doi: 10.1080/10408690091189266
[29]	YU H C, TAN F J. Optimization of ultrasonic-assisted enzymatic hydrolysis conditions for the production of antioxidant hydrolysates from porcine liver by using response surface methodology[J]. Asian-Australasian Journal of Animal Sciences,2017,30(11):1612−1619. doi: 10.5713/ajas.16.0807
[30]	蒋海萍. 蓝圆鲹蛋白制备抗氧化肽的研究[D]. 南宁: 广西大学, 2014. JIANG H P. Preparation of antioxidative peptides from round scad protein[D]. Nanning: Guangxi University, 2014.
[31]	兰颖. 鹿血主要功能成分提取及其微胶囊化研究[D]. 长春: 吉林大学, 2015. LAN Y. Extraction of deer blood mian functional component and its microencapsulation[D]. Changchun: Jilin University, 2015.
[32]	吕小京, 操德群, 徐年军. 响应面试验优化酶解法制备海洋微藻微拟球藻抗氧化肽工艺[J]. 食品科学,2018,39(06):183−188. [LYU X J, CAO D Q, XU N J. Optimization of enzymatic preparation of antioxidant peptides from protein hydrolysate of the marine microalgae Nannochloropsis by response surface methodology[J]. Food Science,2018,39(06):183−188. doi: 10.7506/spkx1002-6630-201806029 LV X J, CAO D Q, XU N J. Optimization of enzymatic preparation of antioxidant peptides from protein hydrolysate of the marine microalgae Nannochloropsis by response surface methodology[J]. Food Science, 2018, 39(06): 183-188. doi: 10.7506/spkx1002-6630-201806029
[33]	苏永昌, 刘淑集, 吴成业. 海参多肽的制备工艺优化及其抗氧化测定[J]. 福建水产,2009(2):6−10. [SU Y C, LIU S J, WU C Y. Preparation process optimization of sea cucumber polypeptide and its antioxidant assay[J]. Joural of Fujian Fisheries,2009(2):6−10. doi: 10.3969/j.issn.1006-5601.2009.02.002 SU Y C, LIU S J, WU C Y. Preparation process optimization of sea cucumber polypeptide and its antioxidant assay[J]. Joural of Fujian Fisheries, 2009(2): 6-10. doi: 10.3969/j.issn.1006-5601.2009.02.002
[34]	于慧, 李明艳, 张典, 等. 响应面试验优化裙带菜蛋白酶解工艺及酶解物抗氧化活性[J]. 食品科学,2017,38(6):96−103. [YU H, LI M Y, ZHANG D, et al. Optimization of enzymatic hydrolysis of Undaria pinnatifida protein and antioxidant activity of its hydrolysate[J]. Food Science,2017,38(6):96−103. doi: 10.7506/spkx1002-6630-201706015 YU H, LI M Y, ZHANG D, et al. Optimization of enzymatic hydrolysis of Undaria pinnatifida protein and antioxidant activity of its hydrolysate[J]. Food Science, 2017, 38(6): 96-103. doi: 10.7506/spkx1002-6630-201706015
[35]	薛园园. 豌豆蛋白的酶水解及其产物抗氧化性能研究[D]. 郑州: 河南工业大学, 2011. XUE Y Y. Enzymatic hydrolysis of pea protein and antioxidative activity of the hydrolysates[D]. Zhengzhou: Henan University of Technology, 2011.
[36]	周丽卿. 鹰嘴豆多肽的制备及其改性研究[D]. 咸阳: 西北农林科技大学, 2012. ZHOU L Q. Study on the preparation and modification of chickpea peptide[D]. Xianyang: Northwest A & F University, 2012.
[37]	LI X, DENG J L, SHEN S A, et al. Antioxidant activities and functional properties of enzymatic protein hydrolysates from defatted Camellia oleifera seed cake[J]. Journal of Food Science and Technology-Mysore,2015,52(9):5681−5690. doi: 10.1007/s13197-014-1693-z
[38]	唐蔚, 宁奇, 孙培冬. 南瓜籽抗氧化肽的制备及分离纯化[J]. 中国油脂,2016,41(2):20−24. [TANG W, NING Q, SUN P D. Preparation, isolation and purification of pumpkin seed antioxidant peptides[J]. China Oils and Fats,2016,41(2):20−24. doi: 10.3969/j.issn.1003-7969.2016.02.006 TANG W, NING Q, SUN P D. Preparation, isolation and purification of pumpkin seed antioxidant peptides[J]. China Oils and Fats, 2016, 41(2): 20-24. doi: 10.3969/j.issn.1003-7969.2016.02.006
[39]	蔡苗苗. 舌状蜈蚣藻抗氧化肽的制备、纯化及结构鉴定[D]. 上海: 上海海洋大学, 2020. CAI M M. Preparation, purification, structure identification of antioxidant peptide from Grateloupia livida[D]. Shanghai: Shanghai Ocean University, 2020.
[40]	CIAN R E, ALAIZ M, VIOQUE J, et al. Enzyme proteolysis enhanced extraction of ACE inhibitory and antioxidant compounds (peptides and polyphenols) from Porphyra columbina residual cake[J]. Journal of Applied Phycology,2013,25(4):1197−1206. doi: 10.1007/s10811-012-9913-2
[41]	RODRIGUEZ D J C, KUROZAWA L E, NETTO F M, et al. Optimization of the enzymatic hydrolysis of blue shark skin[J]. Journal of Food Science,2011,76(7):C938−C949. doi: 10.1111/j.1750-3841.2011.02318.x
[42]	程慧莹, 李贵丽, 韩志萍, 等. 链带藻Desmodesmus sp. QL96蛋白质的营养价值评价与生理活性研究[J]. 热带作物学报,2021,42(5):1440−1447. [CHENG H Y, LI G L, HAN Z P, et al. Evaluation of Desmodesmus sp. QL96 protein on nutritional value and its physiological activity[J]. Chinese Journal of Tropical Crops,2021,42(5):1440−1447. doi: 10.3969/j.issn.1000-2561.2021.05.033 CHENG H Y, LI G L, HAN Z P, et al. Evaluation of Desmodesmus sp. QL96 protein on nutritional value and its physiological activity[J]. Chinese Journal of Tropical Crops, 2021, 42(5): 1440-1447. doi: 10.3969/j.issn.1000-2561.2021.05.033
[43]	漆明. 氨基酸类神经递质与智力及癫痫的关系[J]. 医学信息,2019,32(20):33−36. [QI M. Relationship between amino acid neurotransmitters and intelligence and epilepsy[J]. Medical Information,2019,32(20):33−36. doi: 10.3969/j.issn.1006-1959.2019.20.010 QI M. Relationship between amino acid neurotransmitters and intelligence and epilepsy[J]. Medical Information, 2019, 32(20): 33-36. doi: 10.3969/j.issn.1006-1959.2019.20.010
[44]	黎育颖, 尹杰, 王利剑, 等. 谷氨酸和天冬氨酸对断奶仔猪生长性能和肠道功能的影响[J]. 动物营养学报,2017,29(11):3863−3869. [LI Y Y, YING J, WANG L J, et al. Effects of glutamic acid and aspartic acid on growth performance and intestinal function of weaned piglets[J]. Chinese Journal of Animal Nutrition,2017,29(11):3863−3869. doi: 10.3969/j.issn.1006-267x.2017.11.004 LI Y Y, YING J, WANG L J, et al. Effects of glutamic acid and aspartic acid on growth performance and intestinal function of weaned piglets[J]. Chinese Journal of Animal Nutrition, 2017, 29(11): 3863-3869. doi: 10.3969/j.issn.1006-267x.2017.11.004
[45]	程勇杰, 陈小伟, 张沙沙, 等. 柘树植物酵素中氨基酸分析及抗氧化性能研究[J]. 食品工业科技,2018,39(6):1−7. [CHENG Y J, CHEN X W, ZHAGN S S, et al. Analysis of amino acids andin vitro antioxidant activity of Cudrania tricuspidata Jiaosu[J]. Science and Technology of Food Industry,2018,39(6):1−7. CHENG Y J, CHEN X W, ZHAGN S S, et al. Analysis of amino acids and in vitro antioxidant activity of Cudrania tricuspidata Jiaosu[J]. Science and Technology of Food Industry, 2018, 39(6): 1-7.
[46]	PAVLICEVIC M, MAESTRI E, MARMIROLI M. Marine bioactive peptides-an overview of generation, structure and application with a focus on food sources[J]. Marine Drugs,2020,18(4248):1−21.
[47]	王瑞雪, 孙洋, 钱方. 抗氧化肽及其研究进展[J]. 食品科技,2011,36(5):83−86. [WANG R X, SUN Y, QIAN F. Antioxidant peptides and its research advance[J]. Food Science and Technology,2011,36(5):83−86. WANG R X, SUN Y, QIAN F. Antioxidant peptides and its research advance[J]. Food Science and Technology, 2011, 36(5): 83-86.
[48]	张强, 李伟华. 抗氧化肽的研究现状[J]. 食品与发酵工业,2021,47(2):298−304. [ZHANG Q, LI W H. Research status of antioxidant peptides[J]. Food and Fermentation Industries,2021,47(2):298−304. doi: 10.13995/j.cnki.11-1802/ts.024999 ZHANG Q, LI W H. Research status of antioxidant peptides[J]. Food and Fermentation Industries, 2021, 47(2): 298-304. doi: 10.13995/j.cnki.11-1802/ts.024999
[49]	王乐, 李享, 刘文营, 等. 清酱肉体外模拟消化后粗肽的抗氧化活性和氨基酸分析[J]. 中国食品学报,2021,21(2):319−326. [WANG L, LI X, LIU W Y, et al. Antioxidant activity and amino acid analysis of crude peptides after simulated digestion of clear soy sauce in vitro[J]. Journal of Chinese Institute of Food Science and Technology,2021,21(2):319−326. WANG L, LI X, LIU W Y, et al. Antioxidant activity and amino acid analysis of crude peptides after simulated digestion of clear soy sauce in vitro[J]. Journal of Chinese Institute of Food Science and Technology, 2021, 21(2): 319-326.
[50]	葛晓鸣, 顾伟, 徐永健. 海马水解蛋白的氨基酸组成与抗氧化能力的关系[J]. 核农学报,2019,33(2):322−329. [GE X M, GU W, XU Y J. The relationship between amino acid composition and antioxidant capacity of hippocampal hydrolyzed protein[J]. Journal of Nuclear Agricultural Sciences,2019,33(2):322−329. doi: 10.11869/j.issn.100-8551.2019.02.0322 GE X M, GU W, XU Y J. The relationship between amino acid composition and antioxidant capacity of hippocampal hydrolyzed protein[J]. Journal of Nuclear Agricultural Sciences, 2019, 33(2): 322-329. doi: 10.11869/j.issn.100-8551.2019.02.0322
[51]	SATHEESHKUMAR K, MUGESH G. Synthesis and antioxidant activity of peptide-based ebselen analogues[J]. Chemistry-A European Journal,2011,17(17):4849−4857. doi: 10.1002/chem.201003417
[52]	张晖, 唐文婷, 王立, 等. 抗氧化肽的构效关系研究进展[J]. 食品与生物技术学报,2013,32(7):673−679. [ZHANG H, TANG W T, WANG L, et al. Review on structure-activity relationship of antioxidative peptides[J]. Journal of Food Science and Biotechnology,2013,32(7):673−679. doi: 10.3969/j.issn.1673-1689.2013.07.001 ZHANG H, TANG W T, WANG L, et al. Review on structure-activity relationship of antioxidative peptides[J]. Journal of Food Science and Biotechnology, 2013, 32(7): 673-679. doi: 10.3969/j.issn.1673-1689.2013.07.001

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