Isolation, Identification and Biological Characteristics of Postharvest Pathogens of Yuluxiang Pear

HOU Yaru; GAO Zhenfeng; YANG Zhiguo; CHEN Tian; ZHANG Yang; GUAN Junfeng; ZHANG Lixin; ZHANG Xiaoyu

doi:10.13386/j.issn1002-0306.2021110364

Volume 43 Issue 18

Sep. 2022

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Science and Technology of Food Industry > 2022 > 43(18): 122-129. > DOI: 10.13386/j.issn1002-0306.2021110364

HOU Yaru, GAO Zhenfeng, YANG Zhiguo, et al. Isolation, Identification and Biological Characteristics of Postharvest Pathogens of Yuluxiang Pear[J]. Science and Technology of Food Industry, 2022, 43(18): 122−129. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110364.

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Isolation, Identification and Biological Characteristics of Postharvest Pathogens of Yuluxiang Pear

1.
College of Food Science and Engineering , Shanxi Agricultural University, Taiyuan 030031, China
2.
Institute of Biotechnology and Food Science, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050051, China

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Received Date: November 29, 2021
Available Online: July 04, 2022

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Abstract

Abstract

The rot caused by fungal infection is the main factor affecting the postharvest storage of Yuluxiang pear. In order to clarify the species and biological characteristics of pathogenic bacteria that cause postharvest rot of Yuluxiang pear, the postharvest pathogenic bacteria were isolated and purified, combining morphological and molecular biological characteristics to determine its species, and to clarify the growth of pathogenic bacteria under different temperature, pH, carbon and nitrogen source conditions. The results showed that: Alternaria alternata, Aspergillus, Penicillium polonicum and Fusarium were the main pathogens causing postharvest rot of Yuluxiang pear. The optimum growth temperature of Alternaria alternata and Fusarium was 25~30 ℃, while the optimum growth temperature of Aspergillus and Penicillium polonicum were 25~35 ℃ and 20~25 ℃, respectively. The optimum growth pH of Alternaria alternata and Aspergillus were 6~10 and 7, respectively, while the optimum growth pH of Penicillium polonicum and Fusarium were both 5~7. The optimum growth carbon sources of Alternaria alternata and Penicillium polonicum are glucose and mannitol, respectively, the optimum growth carbon source of Aspergillus and Fusarium was maltose. The optimum growth nitrogen source of Alternaria alternata, Aspergillus and Fusarium was beef extract, and the optimum growth nitrogen source of Penicillium polonicum was peptone.
- Yuluxiang pear,
- disease,
- pathogenic fungi,
- isolation and identification,
- biological characteristics

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[1]	马风丽, 杜艳民, 王阳, 等. 1-MCP 对‘玉露香’梨采后果实品质和叶绿素保持的影响[J]. 园艺学报,2019,46(12):2299−2308. [MA F L, DU Y M, WANG Y, et al. Effects of 1-Methylcyciopropene (1-MCP) on quality and chlorophyll maintenance of postharvest 'Yuluxiang' pear[J]. Acta Horticulturae Sinica,2019,46(12):2299−2308. MA F L, DU Y M, WANG Y, et al. Effects of 1-Methylcyciopropene (1-MCP) on quality and chlorophyll maintenance of postharvest 'Yuluxiang' pear[J]. Acta Horticulturae Sinica, 2019, 46(12): 2299-2308.
[2]	STEFAN P, CHRISTIAN J S, SASKIA D M P, et al. Infection strategies deployed by Botrytis cinerea, Fusarium acuminatum, and Rhizopus stolonifer as a function of tomato fruit ripening stage[J]. Frontiers in Plant Science,2019,10:223. doi: 10.3389/fpls.2019.00223
[3]	STEFAN S, DANIJELA R, ZELJKO S, et al. Penicillium and talaromyces species as postharvest pathogens of pear fruit (Pyrus communis L.) in Serbia[J]. Plant Disease, 2021.
[4]	LEGRAND N N G, QIAN X, YANG Q Y, et al. Securing fruit production: Opportunities from the elucidation of the molecular mechanisms of postharvest fungal infections[J]. Comprehensive Reviews in Food Science and Food Safety, 2021.
[5]	ANTONIOS Z, IOANNIS G, ATHANASIOS T, et al. Metagenomics analysis of fungal communities associated with postharvest diseases in pear fruits under the effect of management practices[J]. Archives of Microbiology, 2020.
[6]	ABDULLAH Q, MAHMOUD A, AL-HARETHI A, et al. A peer-reviewed journal PSM microbiology isolation and identification of fungal post-harvest rot of some fruits in yemen[J]. Plant Pathology & Quarantine,2019,6(1):19−29.
[7]	AL-HINDI R R, AL-NAJADA A R, MOHAMED S A. Isolation and identification of some fruit spoilage fungi: Screening of plant cell wall degrading enzymes[J]. African Journal of Microbiology Research,2011,5(4):443−448.
[8]	YARADUA S S, YUSUF M, KANKARA S L. Isolation and identification of fungi from some selected vegetables in Kankara Local Governmnet Area, Katsina State[J]. UJMR,2018,3:76−80.
[9]	BORECKA H. Fungi of the genus penicillium on apples and pears during the storage period[J]. Acta Agrobotanica,2015,30(2):213−227. doi: 10.5586/aa.1977.016
[10]	VOLSCHENK Q, DU PLESSIS E M, DUVENAGE F J, et al. Effect of postharvest practices on the culturable filamentous fungi and yeast microbiota associated with the pear carpoplane[J]. Postharvest Biology and Technology,2016:118.
[11]	BARKAI-GOLAN R, PASTER N. Mouldy fruits and vegetables as a source of mycotoxins: Part 1[J]. World Mycotoxin Journal,2008,1(2):147−159. doi: 10.3920/WMJ2008.x018
[12]	牛佳佳, 左倩倩, 梁慎, 等. 酥梨贮藏病害病原菌的分离鉴定及防治效果分析[J]. 保鲜与加工,2018,18(6):7−12. [NIU J, ZUO Q Q, LIANG S, et al. Isolation and identification of disease pathogenic bacteria of 'Suli' pear in storage period and analysis on the prevention effeciency[J]. Storage and Process,2018,18(6):7−12. doi: 10.3969/j.issn.1009-6221.2018.06.002 NIU J, ZUO Q Q, LIANG S, et al. Isolation and identification of disease pathogenic bacteria of ‘Suli’ pear in storage period and analysis on the prevention effeciency[J]. Storage and Process, 2018, 18(6): 7-12. doi: 10.3969/j.issn.1009-6221.2018.06.002
[13]	张秀伟, 张鹏, 李用奇. 金刺梨黑斑病病原菌的分离及鉴定[J]. 中国南方果树,2019,48(2):106−109. [ZHANG X W, ZHANG P, LI Y Q. Isolation and identification of the pathogen of black spot of golden thorn pear[J]. South China Fruit Tree,2019,48(2):106−109. ZHANG X W, ZHANG P, LI Y Q. Isolation and identification of the pathogen of black spot of golden thorn pear[J]. South China Fruit Tree, 2019, 48(2): 106-109.
[14]	任美佳, 张华平, 钟聪慧, 等. 库尔勒香梨黑斑病病原鉴定[J]. 石河子大学学报(自然科学版),2020,38(5):574−579. [REN M J, ZHANG H P, ZHONG C H, et al. Identification of the pathogens causing Korla pear black spot in alear[J]. Journal of Shihezi University Natural Science,2020,38(5):574−579. doi: 10.13880/j.cnki.65-1174/n.2020.23.021 REN M J, ZHANG H P, ZHONG C H, et al. Identification of the pathogens causing Korla pear black spot in alear [J]. Journal of Shihezi University Natural Science, 2020, 38(5): 574-579. doi: 10.13880/j.cnki.65-1174/n.2020.23.021
[15]	田艳丽, 胡旭东, 赵玉强, 等. 内蒙古马铃薯黑胫病病原菌的分离和鉴定[J]. 植物病理学报,2018,48(6):721−727. [TIAN N Y L, HU X D, ZHAO Y Q, et al. Isolation and identification of the pathogen causing potato blackleg in Inner Mongolia[J]. Acta Phytopathologyca Sinica,2018,48(6):721−727. TIAN N Y L, HU X D, ZHAO Y Q, et al. Isolation and identification of the pathogen causing potato blackleg in Inner Mongolia[J]. Acta Phytopathologyca Sinica, 2018, 48(6): 721-727.
[16]	LI L, PAN H, CHEN M Y, et al. Isolation and identification of pathogenic fungi causing postharvest fruit rot of kiwifruit (Actinidia chinensis) in China[J]. Journal of Phytopathology, 2017, 165(11−12).
[17]	高振峰, 冯志宏, 赵佳. 红灯樱桃采后主要病原真菌的鉴定及Bacillus velezensis G-1天然产物广谱抑菌效果[J/OL]. 果树学报: 1−17 [2021-09-26]. https://doi.org/10.13925/j.cnki.gsxb.20210128. GAO Z F, FENG Z H, ZHAO J. Identification of main postharvest pathogenic fungi in Hongdeng cherry and the antifungal spectrum of Bacillus velezensis G-1 natural product [J/OL]. Journal of Fruit Science: 1−17[2021-09-26]. https://doi.org/10.13925/j.cnki.gsxb.20210128.
[18]	魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社, 1979 WEI J C. Handbook of fungi identification[M]. Shanghai: Shanghai Science and Technology Press, 1979.
[19]	葛起新, 陈育新, 徐同. 中国真菌志-拟盘多毛孢属[M]. 北京: 科学出版社, 2009 GE Q X, CHEN Y X, XU T. Chinese Fungi Chronicles-Phytochaetium[M]. Beijing: Science Press, 2009.
[20]	李焕宇, 付婷婷, 张云, 等. 5种方法提取真菌基因组DNA作为PCR模板效果的比较[J]. 中国农学通报,2017,33(16):28−35. [LI H Y, FU T T, ZHANG Y, et al. Effect comparison of five methods to extract fungal genomic DNA as PCR templates[J]. Chinese Agricultural Science Bulletin,2017,33(16):28−35. doi: 10.11924/j.issn.1000-6850.casb16060084 LI H Y, FU T T, ZHANG Y, et al. Effect comparison of five methods to extract fungal genomic DNA as PCR templates[J]. Chinese Agricultural Science Bulletin, 2017, 33(16): 28-35. doi: 10.11924/j.issn.1000-6850.casb16060084
[21]	张建航. 花生茎腐病病原菌鉴定与生物学特性及AP2/ERF的生物信息学分析[D]. 郑州: 河南农业大学, 2018 ZHANG J H. Pathogen identification and biological characteristics of peanut collar rot, bioinformatics analysis of AP2/ERF[D]. Zhengzhou: Henan Agricultural University, 2018.
[22]	周倩, 冯肖, 纪淑娟, 等. 蓝莓果实常温贮藏过程中表面病原真菌的分离与鉴定[J]. 中国食品学报,2020,20(2):271−279. [ZHOU Q, FENG X, JI S J, et al. Isolation and identification of surface pathogenic fungi for blueberry during storage at room temperature[J]. Journal Chinese Institute of Food Science and Technology,2020,20(2):271−279. doi: 10.16429/j.1009-7848.2020.02.033 ZHOU Q, FENG X, JI S J, et al. Isolation and identification of surface pathogenic fungi for blueberry during storage at room temperature[J]. Journal Chinese Institute of Food Science and Technology, 2020, 20(2): 271-279. doi: 10.16429/j.1009-7848.2020.02.033
[23]	AL-NAJADA A R, GHERBAWY Y A. Molecular identification of spoilage fungi isolated from fruit and vegetables and their control with chitosan[J]. Food Biotechnology,2015,29(2):166−184. doi: 10.1080/08905436.2015.1027222
[24]	刘行风, 刘东, 陶磊, 等. 黑龙江省黄瓜链格孢叶斑病病原鉴定及生物学特性研究[J]. 中国蔬菜,2021(8):80−86. [LIU X F, LIU D, TAO L, et al. Pathogen identification and biological characteristics of cucumber alternaria leaf spot of in Heilongjiang Province[J]. Chinese Vegetables,2021(8):80−86. doi: 10.19928/j.cnki.1000-6346.2021.1034 LIU X F, LIU D, TAO L, et al. Pathogen identification and biological characteristics of cucumber alternaria leaf spot of in Heilongjiang Province[J]. Chinese Vegetables, 2021(8): 80-86. doi: 10.19928/j.cnki.1000-6346.2021.1034
[25]	张驰成. 热带牧草真菌病害调查、病原鉴定及基础生物学特性研究[D]. 海口: 海南大学, 2016 ZHANG C C. Survey, identification and biological characteristics determination of tropical forage fungal diseases [D]. Haikou: Hainan University, 2016.
[26]	王雪, 尹桥秀, 李冬雪, 等. 茶褐枯病病原菌(Colletotrichum camelliae)生物学特性研究[J]. 中国植保导刊,2019,39(6):5−11. [WANG X, YIN Q X, LI D X, et al. Biological characteristics of Colletotrichum camelliae causing tea brown blight disease[J]. China Plant Protection,2019,39(6):5−11. doi: 10.3969/j.issn.1672-6820.2019.06.001 WANG X, YIN Q X, LI D X, et al. Biological characteristics of Colletotrichum camelliae causing tea brown blight disease[J]. China Plant Protection, 2019, 39(6): 5-11. doi: 10.3969/j.issn.1672-6820.2019.06.001
[27]	马文娟, 梁向东, 刘月廉. 广西玉林西番莲果腐病病原菌的分离与鉴定[J]. 黑龙江农业科学,2021(10):43−45, 54. [MA W J, LIANG X D, LIU Y L. Isolation and identification of the pathogen of passion fruit rot in Yulin, Guangxi[J]. Heilongjiang Agricultural Sciences,2021(10):43−45, 54. MA W J, LIANG X D, LIU Y L. Isolation and identification of the pathogen of passion fruit rot in Yulin, Guangxi[J]. Heilongjiang Agricultural Sciences, 2021(10): 43-45, 54.
[28]	周英. 奉新猕猴桃3种真菌病害病原鉴定、生物学特性及药剂筛选研究[D]. 南昌: 江西农业大学, 2019 ZHOU Y. Identification, biological characteristics and fungicide screening of 3 fungal pathogens from kiwifruit in Fengxi County [D]. Nanchang: Jiangxi Agricultural University, 2019.
[29]	朱从一. 柑橘采后青霉属病原菌生物学特性及其系统发育[D]. 武汉: 华中农业大学, 2011 [ZHU C Y, The biological characteristics and molecular phylogeny analyses of Penicillium spp. that causing the mold fruit of citrus. [D]. Wuhan: Huazhong Agricultural University, 2011.
[30]	伍晓丽, 王钰, 刘飞, 等. 黄连根腐病镰刀菌属病原真菌鉴定[J]. 中国中药杂志,2020,45(6):1323−1328. [WU X L, WANG Y, LIU F, et al. Identification of the pythium pathogen responsible for root rot of Coptis chinensis[J]. China Journal of Chinese Materia Medica,2020,45(6):1323−1328. doi: 10.19540/j.cnki.cjcmm.20200112.102 [WU X L, WANG Y, LIU F, et al. Identification of the pythium pathogen responsible for root rot of Coptis chinensis[J]. China Journal of Chinese Materia Medica, 2020, 45(6): 1323- doi: 10.19540/j.cnki.cjcmm.20200112.102

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