Optimization of Extraction Process, Isolation and Characterization of Mycosporine-Like Amino Acids from Four Species of Marine Red Macroalgaes

HAN Xiu; QIAN Liangliang; CHENG Tongjie; SHEN Yan; DENG Xiaoqun; ZHU Wenxuan; SUN Yingying

doi:10.13386/j.issn1002-0306.2021110358

Volume 43 Issue 18

Sep. 2022

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Science and Technology of Food Industry > 2022 > 43(18): 208-216. > DOI: 10.13386/j.issn1002-0306.2021110358

HAN Xiu, QIAN Liangliang, CHENG Tongjie, et al. Optimization of Extraction Process, Isolation and Characterization of Mycosporine-Like Amino Acids from Four Species of Marine Red Macroalgaes[J]. Science and Technology of Food Industry, 2022, 43(18): 208−216. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110358.

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Optimization of Extraction Process, Isolation and Characterization of Mycosporine-Like Amino Acids from Four Species of Marine Red Macroalgaes

1.
Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China
2.
Lianyungang Comprehensive Inspection and Testing Center for Quality and Technology, Lianyungang 222006, China

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Received Date: November 29, 2021
Available Online: July 07, 2022

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Abstract

Abstract

Marine macroalgae is one of the good sources of mycosporine-like amino acids (MAAs), especially red macroalgae. As a new type of active substances with commercial development prospect, extraction, isolation and characterization of MAAs are far from enough for the application. To obtain the extraction process of MAAs from four species of red macroalgae (Bangia fusco-purpurea, Gelidium amansii, Gracilaria confervoides, and Gracilaria sp.), a series of single-factor and orthogonal experiments were carried out to analyze the effects of extraction temperature, time, times and solid-liquid ratio on the yields of MAAs. The extraction process of MAAs was optimized, and the extracts were isolated and identified. The optimized extraction processes of MAAs were obtained as follows: 45 ℃, 3 h, 4 times, 1:25 g/mL. 45 ℃, 1 h, 4 times, 1:20 g/mL. 45 ℃, 2 h, 3 times, 1:15 g/mL. 40 ℃, 1 h, 3 times, 1:20 g/mL. Four MAAs extracts of red algae were prepared by using the above extraction process, and the yields were 249.3, 197.9, 146.4 and 449.5 mg/g, respectively. All of them showed UV absorption characteristics and color reaction of MAAs in UV spectrum and thin layer chromatography (TLC). Furthermore, MAAs extracts from Bangia fusco-purpurea (or Gracilaria sp.) were isolated by silica gel column chromatography to obtain one fraction H1 (or two fractions J1 and J2). MAAs composition and proportion of these fractions were determined by UV, HPLC and MS, and comparison with existing literatures. H1 included shinorine, palythine and porphyra-334 (the content of these three MAAs was 95.4% of the content of the fraction H1), and palythenic acid (4.6%). Shinorine, palythine, porphyra-334 (96.3% of the fraction J1), and palythenic acid (3.7%) were in J1. And J2 was palythine.
- mycosporine-like amino acids,
- red macroalgae,
- extraction process,
- isolation,
- characterization

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